CRM1 Antibody

Images

 
Western Blot: CRM1 Antibody [NB100-79802] - Detection of Human and Mouse CRM1 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: ...read more
Immunohistochemistry: CRM1 Antibody [NB100-79802] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: CRM1 Antibody [NB100-79802] - A) Whole cell lysate from HeLa (5, 15 and 50 mcg), Ramos (50 mcg) and mouse NIH3T3 (50 mcg) cells. B) Whole cell lysate (1 mg/IP; 1/4 of reaction loaded/lane) from HeLa cells. ...read more
Immunohistochemistry: CRM1 Antibody [NB100-79802] - FFPE section of mouse squamous cell carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802] - Human metastatic lymph node. Antibody used at a dilution of 1:5,000 (0.2ug/ml).
Immunohistochemistry: CRM1 Antibody [NB100-79802] - Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Simple Western: CRM1 Antibody [NB100-79802] - Simple Western lane view shows a specific band for CRM1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more
Proximity Ligation Assay: CRM1 Antibody [NB100-79802] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human ATM (NB100-271) and rabbit anti-human CRM1 (NB100-79802). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: CRM1 Antibody [NB100-79802] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human CHK1 (NB100-274) and rabbit anti-human CRM1 (NB100-79802). Image merged from DAPI (2ms) and Texas ...read more
Proximity Ligation Assay: CRM1 Antibody [NB100-79802] - Secondary-conjugate Duolink In Situ PLA in Hela cells. goat anti-human CHK1 (NB100-311) and rabbit anti-human CRM1 (NB100-79802). Image merged from DAPI (2MS), ...read more
Proximity Ligation Assay: CRM1 Antibody [NB100-79802] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human RCC1 (NB110-40590) and rabbit anti-human CRM1 (NB100-79802). Image merged from DAPI (2ms) and ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

CRM1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1025 and the C-terminus (residue 1071) of human Chromosome Region Maintenance 1 (Exportin 1) (NP_003391.1).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
XPO1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:15000
  • Simple Western 1:500
  • Immunocytochemistry/Immunofluorescence 1:1000 to 1:10000
  • Immunohistochemistry 1:2000 to 1:10000
  • Immunohistochemistry-Paraffin 1:2000 to 1:10000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Proximity Ligation Assay 1:1000-1:10000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
123 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-79802 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CRM1 Antibody

  • Chromosome region maintenance 1 protein homolog
  • DKFZp686B1823
  • emb
  • exp1
  • exportin 1 (CRM1 homolog, yeast)
  • exportin 1 (CRM1, yeast, homolog)
  • Exportin-1 (required for chromosome region maintenance)
  • exportin-1
  • yeast, homolog

Background

CRM1 mediates the nuclear export of cellular proteins (cargoes) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, CRM1 binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The direction of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. CRM1 also mediates the nuclear export of the Rev protein of HIV-1 and Rex protein of HTLV-1. It is involved in Rex dimerization, U3 snoRNA transport from Cajal bodies to nucleoli. CRM1 is also involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. CRM1 is also involved in the regulation NFAT and AP-1. Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Active transport of molecules through the NPC is a signal mediated process that is not well understood. Proteins to be exported from the nucleus to cytoplasm contain leucine-rich nuclear export signals (NESs). NESs are recognized by transport receptors belonging to the importin-beta family of proteins. It has been shown that NES export is mediated by CRM 1 (Chromosome Region Maintenance 1), an importin-beta family member that binds NESs, Ran:GTP, and Ran binding protein (RanBP). CRM 1 mediates the nuclear exportation of numerous proteins, including histone deacetylases (HDACs), viral proteins, immunophilins, and STAT 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-79802
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP, PLA

Publications for CRM1 Antibody (NB100-79802)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for CRM1 Antibody (NB100-79802)

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Blogs on CRM1.

Survivin Acetylation: Affecting Apoptosis and Cancer
Survivin (BRIC5) is an inhibitor of apoptosis that also promotes cellular adaptation under stressful conditions and helps to regulate cell division. Recently, an antibody study by Dr. H Wang et al. at Brown University [PMID: 20826784] found that Survi...  Read full blog post.

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Bioinformatics

Gene Symbol XPO1
Entrez
Uniprot