CRM1 Antibody

Images

 
Biological Strategies: Western Blot: CRM1 Antibody [NB100-79802] - Detection of Human and Mouse CRM1 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, and ...read more
Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802] - Mouse teratoma. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Independent Antibodies: Western Blot: CRM1 Antibody [NB100-79802] - A) Whole cell lysate from HeLa (5, 15 and 50 mcg), Ramos (50 mcg) and mouse NIH3T3 (50 mcg) cells. B) Whole cell lysate (1 mg/IP; 1/4 of ...read more
Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802] - Human lung cancer. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Simple Western: CRM1 Antibody [NB100-79802] - Simple Western lane view shows a specific band for CRM1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

CRM1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1025 and the C-terminus (residue 1071) of human Chromosome Region Maintenance 1 (Exportin 1) (NP_003391.1).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
XPO1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:15000
  • Simple Western 1:500
  • Immunocytochemistry/Immunofluorescence 1:1000 to 1:10000
  • Immunohistochemistry 1:2000 to 1:10000
  • Immunohistochemistry-Paraffin 1:2000 to 1:10000
  • Immunoprecipitation 2-10 ug/mg lysate
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Theoretical MW
123 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB100-79802 in the following applications:

  • 3 publications
  • WB
    4 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CRM1 Antibody

  • Chromosome region maintenance 1 protein homolog
  • DKFZp686B1823
  • emb
  • exp1
  • exportin 1 (CRM1 homolog, yeast)
  • exportin 1 (CRM1, yeast, homolog)
  • Exportin-1 (required for chromosome region maintenance)
  • exportin-1
  • yeast, homolog

Background

CRM1 mediates the nuclear export of cellular proteins (cargoes) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, CRM1 binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The direction of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. CRM1 also mediates the nuclear export of the Rev protein of HIV-1 and Rex protein of HTLV-1. It is involved in Rex dimerization, U3 snoRNA transport from Cajal bodies to nucleoli. CRM1 is also involved in the control of several cellular processes by controlling the localization of cyclin B, MPAK, and MAPKAP kinase 2. CRM1 is also involved in the regulation NFAT and AP-1. Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC's. NPC's contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Active transport of molecules through the NPC is a signal mediated process that is not well understood. Proteins to be exported from the nucleus to cytoplasm contain leucine-rich nuclear export signals (NESs). NESs are recognized by transport receptors belonging to the importin-beta family of proteins. It has been shown that NES export is mediated by CRM 1 (Chromosome Region Maintenance 1), an importin-beta family member that binds NESs, Ran:GTP, and Ran binding protein (RanBP). CRM 1 mediates the nuclear exportation of numerous proteins, including histone deacetylases (HDACs), viral proteins, immunophilins, and STAT 1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP

Publications for CRM1 Antibody (NB100-79802)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, WB.


Filter By Application
ICC/IF
(3)
WB
(4)
All Applications
Filter By Species
Human
(3)
Mouse
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-79802 Applications Species
Garcia-Aguirre I, Alamillo-Iniesta A, Rodriguez-Perez R et al. Enhanced nuclear protein export in premature aging and rescue of the progeria phenotype by modulation of CRM1 activity Aging Cell Jul 15 2019 [PMID: 31305018] (WB, ICC/IF, Human) WB, ICC/IF Human
Shakhparonov MI, Antipova NV, Shender VO. Expression and Intracellular Localization of Paraoxonase 2 in Different Types of Malignancies. Acta naturae. [PMID: 30397533] (ICC/IF, Human) ICC/IF Human
Velez-Aguilera G, de Dios Gomez-Lopez J, Jimenez-Gutierrez GE et al. Control of nuclear beta-dystroglycan content is crucial for the maintenance of nuclear envelope integrity and function Biochim. Biophys. Acta 2017 Nov 21 [PMID: 29175376] (WB, Mouse) WB Mouse
Vercruysse T, De Bie J, Neggers J et al. The second-generation exportin-1 inhibitor KPT-8602 demonstrates potent activity against acute lymphoblastic leukemia. Clin. Cancer Res. Oct 25 2016 [PMID: 27780859]
Oka M, Mura S, Yamada K et al. Chromatin-prebound Crm1 recruits Nup98-HoxA9 fusion to induce aberrant expression of Hox cluster genes. Elife 2016 Jan 07 [PMID: 26740045] (WB) WB
Oka M, Asally M, Yasuda Y et al. The mobile FG nucleoporin Nup98 is a cofactor for Crm1-dependent protein export. Mol Biol Cell 2010 Jun 1 [PMID: 20375145] (ICC/IF, WB, Mouse, Human) ICC/IF, WB Mouse, Human
von Knethen A, Tzieply N, Jennewein C et al. Casein-kinase-II-dependent phosphorylation of PPARgamma provokes CRM1-mediated shuttling of PPARgamma from the nucleus to the cytosol. J Cell Sci 2010 Jan [PMID: 20026644]

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CRM1 Antibody (NB100-79802) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-79802AF350
Alexa Fluor 405 NB100-79802AF405
Alexa Fluor 488 NB100-79802AF488
Alexa Fluor 532 NB100-79802AF532
Alexa Fluor 594 NB100-79802AF594
Alexa Fluor 647 NB100-79802AF647
Alexa Fluor 700 NB100-79802AF700
Alexa Fluor 750 NB100-79802AF750
Biotin NB100-79802B
DyLight 350 NB100-79802UV
DyLight 405 NB100-79802V
DyLight 488 NB100-79802G
DyLight 550 NB100-79802R
DyLight 594 NB100-79802DL594
DyLight 650 NB100-79802C
DyLight 680 NB100-79802FR
DyLight 755 NB100-79802IR
FITC NB100-79802F
HRP NB100-79802H
Janelia Fluor 549 NB100-79802JF549
Janelia Fluor 646 NB100-79802JF646

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Blogs on CRM1.

Survivin Acetylation: Affecting Apoptosis and Cancer
Survivin (BRIC5) is an inhibitor of apoptosis that also promotes cellular adaptation under stressful conditions and helps to regulate cell division. Recently, an antibody study by Dr. H Wang et al. at Brown University [PMID: 20826784] found that Survi...  Read full blog post.

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Bioinformatics

Gene Symbol XPO1
Entrez
Uniprot
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