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RanBP1 Antibody

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 (2 Reviews)
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Western Blot: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP1 antibody NB100-79814 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Biological Strategies: Western Blot: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. ...read more
Independent Antibodies: Western Blot: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 on HeLa whole cell lysate using NB100-79814. RanBP1 was also immunoprecipitated using rabbit anti-RanBP1 antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

  

Biological Strategies

      

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NB100-79814AF405
Alexa Fluor 488
NB100-79814AF488
Alexa Fluor 647
NB100-79814AF647
Alexa Fluor 700
NB100-79814AF700
Biotin
NB100-79814B
DyLight 350
NB100-79814UV
DyLight 405
NB100-79814V
DyLight 488
NB100-79814G
DyLight 550
NB100-79814R
DyLight 650
NB100-79814C
DyLight 680
NB100-79814FR
DyLight 755
NB100-79814IR
FITC
NB100-79814F
HRP
NB100-79814H
Janelia Fluor 549
NB100-79814JF549
Janelia Fluor 646
NB100-79814JF646
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RanBP1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human RAN Binding Protein 1 using the numbering given in entry NP_002873.1 (GeneID 5902).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RANBP1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunohistochemistry 1:100-1:2000
  • Immunohistochemistry-Paraffin 1:100-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
IHC-P, IHC application reported in customer review.
Control
RanBP1 Lysate (NBL1-15139)
RanBP1 Lysate (NBP2-65579)
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-79814 in the following applications:
Publications
Read Publications using
NB100-79814 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28821644).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RanBP1 Antibody

  • HTF9A
  • MGC88701
  • RAN binding protein 1
  • Ran-binding protein 1
  • RanBP1
  • ran-specific GTPase-activating protein

Background

Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of HepG2 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemistry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of paraffin-embedded stomach cancer tissues using RAN mouse mAb with DAB staining.
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC
RCC1 Antibody
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Western Blot: RCC1 Antibody [NBP1-85638] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/Immunofluorescence: RCC1 Antibody [NBP1-85638] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: RCC1 Antibody [NBP1-85638] - Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
(1 Publication)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
RanBP2 Antibody
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Western Blot: RanBP2 Antibody [NB100-93337] - Whole cell lysate (50 ug) from NIH3T3 and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP2 antibodyused for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: RanBP2 Antibody [NB100-93337] - Detection of Human RanBP2 on HeLa whole cell lysate using NB100-93337. RanBP2 was also immunoprecipitated by rabbit anti-RanBP2 antibody NB100-93338.
Western Blot: RanBP2 Antibody [NB100-93337] - Detection of Human RanBP2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP2 antibody NB100-93337 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
(1 Publication)
Species: Hu, Mu
Applications: WB, IP
CRM1 Antibody
NB100-79802
Western Blot: CRM1 Antibody [NB100-79802] - Detection of Human and Mouse CRM1 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CRM1 antibody NB100-79802 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: CRM1 Antibody [NB100-79802] - Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: CRM1 Antibody [NB100-79802] - A) Whole cell lysate from HeLa (5, 15 and 50 mcg), Ramos (50 mcg) and mouse NIH3T3 (50 mcg) cells. B) Whole cell lysate (1 mg/IP; 1/4 of reaction loaded/lane) from HeLa cells. NB100-79802 used at 0.1 mcg/ml for WB (A and B) and at 3 mcg/mg lysate for IP. CRM1 was also less efficiently immunoprecipitated by NB100-79811, which recognize upstream epitopes on CRM1.
(4 Publications)
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
RanGAP1 Antibody
(OTI1B4)
NBP2-02623
Western Blot: RanGAP1 Antibody (1B4) [NBP2-02623] Analysis of extracts (35ug) from 9 different cell lines by using anti-RanGAP1 monoclonal antibody.
Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody (1B4) [NBP2-02623] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RanGAP1.
Immunohistochemistry-Paraffin: RanGAP1 Antibody (1B4) [NBP2-02623] - Staining of paraffin-embedded Human prostate tissue using anti-RanGAP1 mouse monoclonal antibody.
Species: Hu, Rt, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P
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AF6260
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and 293T human embryonic kidney cell line. PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human SOS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6260) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOS2 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
Applications: WB
SOS1 Antibody
(SOS-01)
NB110-68800
Western Blot: SOS1 Antibody (SOS-01) [NB110-68800] - Analysis (reducing conditions) of human Sos using anti-human Sos (SOS-1). Lane 1: K562 human Caucasian chronic myeloid leukemia cell line Lane 2: RAJI human Burkitt lymphoma cell line
Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in murine transformed fibroblasts using anti-Sos (SOS-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).
Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in human primary fibroblasts using anti-Sos (SOS-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Species: Hu, Mu
Applications: WB, ICC/IF
RASGRF1 Antibody
NBP1-85727
Immunocytochemistry/Immunofluorescence: RASGRF1 Antibody [NBP1-85727] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RASGRF1 Antibody [NBP1-85727] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
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(196908)
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Nestin was detected in immersion fixed human fetal neural progenitor cells using 10 µg/mL Human Nestin Monoclonal Antibody (Catalog # MAB1259) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
(4 Reviews)
(53 Publications)
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Niemann-Pick C1
Antibody
NB400-148
Knockout Validated: Niemann-Pick C1 Antibody [NB400-148] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NPC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human NPC1 Polyclonal Antibody (Catalog # NB400-148) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NPC1 at approximately 240-260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: Niemann-Pick C1 Antibody [NB400-148] - Hela lysate, BHK lysate, A431 lysate tested at 1:1000. Image provided by verified customer review.
Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody [NB400-148] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Niemann-Pick C1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
(3 Reviews)
(33 Publications)
Species: Hu, Mu, Rt, Po, ChHa, Pm
Applications: WB, EM, ICC/IF, IHC, IHC-P, KO
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Western Blot: AGFG2 Antibody [NBP1-83213] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
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Immunohistochemistry-Paraffin: AGFG2 Antibody [NBP1-83213] - Staining of human cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells.
Species: Hu
Applications: WB, IHC, IHC-P
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Western Blot: NTF2 Antibody [NBP1-82520] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: NTF2 Antibody [NBP1-82520] - Immunofluorescent staining of human cell line U-2 OS shows localization to microtubules.
Immunohistochemistry-Paraffin: NTF2 Antibody [NBP1-82520] - Staining in human testis and pancreas tissues using anti-NUTF2 antibody. Corresponding NUTF2 RNA-seq data are presented for the same tissues.
Species: Hu, Mu, Rt
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p120-catenin
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Western Blot: p120-catenin Antibody [NBP1-85383] - Analysis in human cell lines A-431 and HEK293 using Anti-CTNND1 antibody. Corresponding CTNND1 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Immunocytochemistry/Immunofluorescence: p120-catenin Antibody [NBP1-85383] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: p120-catenin Antibody [NBP1-85383] - Staining of human small intestine shows cytoplasmic and membranous positivity in glandular cells.
(1 Publication)
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NUP214 Antibody
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Western Blot: NUP214 Antibody [NB100-2244] - Detection of Human NUP214 on HeLa whole cell lysate using NB100-2244. NUP214 was also immunoprecipitated using rabbit anti-NUP214 antibody BL2244 NB100-2245.
Immunocytochemistry/Immunofluorescence: NUP214 Antibody [NB100-2244] - Affinity purified rabbit anti-NUP214 (NB100-2244) used at a dilution of 1:100 (2ug/ml). Detection: Green fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody FITC conjugated used at a dilution of 1:100.
(1 Publication)
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Applications: WB, ICC/IF, IHC, IHC-P, IP, ICC, IF
S100A10 Antibody
[Unconjugated]
AF2377
Western blot shows lysates of mouse lung and MEF mouse embryonic feeder cells. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100A10 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
S100A10 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Simple Western lane view shows lysates of mouse lung tissue and MEF mouse embryonic feeder cells, loaded at 0.2 mg/mL. A specific band was detected for S100A10 at approximately 11 kDa (as indicated) using 1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
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Applications: WB, Simple Western, IHC
Retinoblastoma
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Western Blot: Retinoblastoma binding protein 1 Antibody [NBP1-26616] - Whole cell lysate from HeLa cells. ARID4A was also immunoprecipitated by rabbit anti-ARID4A antibody NBP1-26617.
Species: Hu
Applications: WB, IP
EXOSC3 Antibody
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Western Blot: EXOSC3 Antibody [NBP2-22261] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22261 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: EXOSC3 Antibody [NBP2-22261] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22261 used for IP at 6 ug/mg lysate. EXOSC3 was also immunoprecipitated by another rabbit anti-EXOSC3 antibody. For blotting immunoprecipitated EXOSC3, NBP2-22261 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
(1 Review)
(1 Publication)
Species: Hu, Mu, Ch
Applications: WB, IP
RanBP1 Antibody
NB100-79814
RanBP1 Antibody
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP

Publications for RanBP1 Antibody (NB100-79814)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


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Reviews for RanBP1 Antibody (NB100-79814) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-79814:
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(1)
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Images Ratings Applications Species Date Details
Immunofluorescence RanBP1 NB100-79814
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reviewed by:
Anonymous
 IF Human 04/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedHEK293
SpeciesHuman
LotA-2

Blocking

Blocking Details3% BSA in PBS; 1h at room temperature

Primary Anitbody

Dilution RatioDilution: 1:400; Buffer: 3% BSA in PBS; 1h at room temperature

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG AlexaFluor 633
Secondary Manufacturer Cat#A-21070
Secondary Concentration4 ug/mL

Details

Detection NotesConfocal microscopy; Zeiss LSM 710 inverted confocal laser scanning microsope
Fixation DetailsFixation: 4% Paraformaldehyde in PBS 15 min; Permeabilization: 0,1% Triton X-100 in PBS 5 min
Wash Description3 x 5 min with PBS
Immunohistochemistry-Paraffin RanBP1 NB100-79814
Enlarge
reviewed by:
Maja Stalekar
 IHC-P Mouse 10/09/2013
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse spinal cord
SpeciesMouse
LotA-2
Commentsheat induced epitope retrieval with Tris-EDTA, pH 9, 20 min. Permeabilization: 0,2% triton X-100 in PBS, 45 min

Blocking

Blocking Details3% BSA in PBS, 1 h, RT

Primary Anitbody

Dilution Ratio1:800 in blocking solution, overnight, 4C

Secondary Antibody

Secondary Descriptiongoat anti-rabbit Alexa Fluor 555
Secondary Manufacturer Cat#Invitrogen, A21428
Secondary Concentration1:1000

Details

Detection Notesconfocal microscopy
Fixation Details4% paraformaldehyde perfusion and postfixation in 10% phosphate-buffered formalin, paraffin embedding, rehydration, antigen retr
Wash DescriptionPBS, 3x5 min

Comments

Commentsheat induced epitope retrieval with Tris-EDTA, pH 9, 20 min. Permeabilization: 0,2% triton X-100 in PBS, 45 min

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for RanBP1 Antibody (NB100-79814) (0)

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Control Lysate(s)

RanBP1 Overexpression Lysate (Native)
RanBP1 Knockout 293T Cell Lysate

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Immunofluorescence RanBP1 NB100-79814
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 Anonymous
04/16/2015
Application: IF
Species: Human
read this review
Immunohistochemistry-Paraffin RanBP1 NB100-79814
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 Maja Stalekar
10/09/2013
Application: IHC-P
Species: Mouse
read this review

Bioinformatics

Gene Symbol RANBP1
Entrez
Uniprot