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RanBP1 Antibody

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Western Blot: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP1 antibody NB100-79814 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Biological Strategies: Western Blot: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. ...read more
Independent Antibodies: Western Blot: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 on HeLa whole cell lysate using NB100-79814. RanBP1 was also immunoprecipitated using rabbit anti-RanBP1 antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

  

Biological Strategies

      

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-79814AF350
Alexa Fluor 405
NB100-79814AF405
Alexa Fluor 488
NB100-79814AF488
Alexa Fluor 532
NB100-79814AF532
Alexa Fluor 594
NB100-79814AF594
Alexa Fluor 647
NB100-79814AF647
Alexa Fluor 700
NB100-79814AF700
Alexa Fluor 750
NB100-79814AF750
Biotin
NB100-79814B
DyLight 350
NB100-79814UV
DyLight 405
NB100-79814V
DyLight 488
NB100-79814G
DyLight 550
NB100-79814R
DyLight 594
NB100-79814DL594
DyLight 650
NB100-79814C
DyLight 680
NB100-79814FR
DyLight 755
NB100-79814IR
FITC
NB100-79814F
HRP
NB100-79814H
Janelia Fluor 549
NB100-79814JF549
Janelia Fluor 646
NB100-79814JF646
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RanBP1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human RAN Binding Protein 1 using the numbering given in entry NP_002873.1 (GeneID 5902).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RANBP1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunohistochemistry 1:100-1:2000
  • Immunohistochemistry-Paraffin 1:100-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
IHC-P, IHC application reported in customer review.
Control
RanBP1 Overexpression Lysate
RanBP1 Knockout 293T Cell Lysate
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-79814 in the following applications:
Publications
Read Publications using
NB100-79814 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28821644).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RanBP1 Antibody

  • HTF9A
  • MGC88701
  • RAN binding protein 1
  • Ran-binding protein 1
  • RanBP1
  • ran-specific GTPase-activating protein

Background

Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of HepG2 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of paraffin-embedded stomach cancer tissues using RAN mouse mAb with DAB staining.
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
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Western Blot: RCC1 Antibody [NBP1-85638] - Analysis using Anti-RCC1 antibody NBP1-85638 (A) shows similar pattern to independent antibody NBP1-85637 (B).
Immunocytochemistry/Immunofluorescence: RCC1 Antibody [NBP1-85638] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: RCC1 Antibody [NBP1-85638] - Staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
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Species: Hu, Mu, Rt
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Western Blot: RanBP2 Antibody [NB120-2938] - Effect of hypoxia on the interaction between HIF1alpha and BICD1. Co-immunoprecipitation of HIF1alpha, Dynein IC, Importin alpha3, RanBP2 with IgG and BICD1 antibodies were shown in left panel. Total protein expressions in lysate were shown in right panel. n = 3. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41418-018-0241-1) licensed under a CC-BY licence.
Immunohistochemistry: RanBP2 Antibody [NB120-2938] - Figure 1 illustrates immunohistochemical staining of RanBP2 in bovine retina using NB120-2938. Figure 2 illustrates Western blot detection of RanBP2 from human retinal extracts using NB120-2938.
Western Blot: RanBP2 Antibody [NB120-2938] - Analysis of human retinal extracts.
(2 Publications)
Species: Hu, Mu, Bv
Applications: WB, IHC, IHC-P
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NB100-79802
Western Blot: CRM1 Antibody [NB100-79802] - Detection of Human and Mouse CRM1 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CRM1 antibody NB100-79802 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry/Immunofluorescence: CRM1 Antibody [NB100-79802] - Association between Nup98-HoxA9 and CRM1 is critical for the Hox Gene activation mediated by Nup98-HoxA9. (B) The effect of LMB treatment on the cellular localization of Nup98-HoxA9. Nup98-HoxA9 ES cells were cultured either in the presence or absence of 5 nM LMB for 2 hr, fixed and stained with antibodies against FLAG (M2) and CRM1. Merged images of FLAG (green) and CRM1 (red) are shown. Nuclei were stained with DAPI. Bar, 10um. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/09540) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802] - Mouse teratoma. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
(8 Publications)
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
RanGAP1 Antibody
(OTI1B4)
NBP2-02623
Western Blot: RanGAP1 Antibody (1B4) [NBP2-02623] Analysis of extracts (35ug) from 9 different cell lines by using anti-RanGAP1 monoclonal antibody.
Immunocytochemistry/Immunofluorescence: RanGAP1 Antibody (1B4) [NBP2-02623] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY RanGAP1.
Immunohistochemistry-Paraffin: RanGAP1 Antibody (1B4) [NBP2-02623] - Staining of paraffin-embedded Human prostate tissue using anti-RanGAP1 mouse monoclonal antibody.
Species: Hu, Rt, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P
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Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and 293T human embryonic kidney cell line. PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human SOS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6260) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOS2 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
Applications: WB
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(SOS-01)
NB110-68800
Western Blot: SOS1 Antibody (SOS-01) [NB110-68800] - Analysis (reducing conditions) of human Sos using anti-human Sos (SOS-1). Lane 1: K562 human Caucasian chronic myeloid leukemia cell line Lane 2: RAJI human Burkitt lymphoma cell line
Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in murine transformed fibroblasts using anti-Sos (SOS-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).
Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in human primary fibroblasts using anti-Sos (SOS-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Species: Hu, Mu
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Western Blot: RASGRF1 Antibody [NBP2-95233] - Analysis of extracts of mouse brain, using RASGRF1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure tim
Immunocytochemistry/Immunofluorescence: RASGRF1 Antibody [NBP2-95233] - Analysis of U2OS cells using RASGRF1. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: RASGRF1 Antibody [NBP2-95233] - Paraffin-embedded human stomach using RASGRF1.
Species: Hu, Mu
Applications: WB, ICC/IF, IHC
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(196908)
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(5 Reviews)
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Niemann-Pick C1
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NB400-148
Knockout Validated: Niemann-Pick C1 Antibody [NB400-148] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NPC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human NPC1 Polyclonal Antibody (Catalog # NB400-148) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NPC1 at approximately 240-260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
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(5 Reviews)
(41 Publications)
Species: Hu, Mu, Rt, Po, ChHa, Ha, Pm
Applications: WB, EM, ICC/IF, IHC, IHC-P, IP, KD, KO
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Western Blot: AGFG2 Antibody [NBP1-83213] - Analysis in control (vector only transfected HEK293T lysate) and AGFG2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
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Species: Hu
Applications: WB, IHC, IHC-P
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Applications: ICC/IF, IHC, IHC-P
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(1 Publication)
Species: Hu, Mu, Rt
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Western Blot: NUP214 Antibody [NB100-2244] - Whole cell lysate from HeLa (5, 15 and 50 ug), and HEK293T (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUP214 antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30seconds.
Immunohistochemistry-Paraffin: NUP214 Antibody [NB100-2244] - Section of ovarian carcinoma. Antibody: Affinity purified rabbit anti- NUP214 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: NUP214 Antibody [NB100-2244] - Section of ovarian carcinoma. Antibody: Affinity purified rabbit anti- NUP214 used at a dilution of 1:200 (1ug/ml). Detection: DAB
(1 Publication)
Species: Hu
Applications: WB, IHC, IHC-P, IP
S100A10 Antibody
[Unconjugated]
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Western blot shows lysates of mouse lung and MEF mouse embryonic feeder cells. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100A10 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
S100A10 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Simple Western lane view shows lysates of mouse lung tissue and MEF mouse embryonic feeder cells, loaded at 0.2 mg/mL. A specific band was detected for S100A10 at approximately 11 kDa (as indicated) using 1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
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Retinoblastoma
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Western Blot: Retinoblastoma binding protein 1 Antibody [NBP1-26616] - Detection of human ARID4A by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID4A antibody NBP1-26616 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: Retinoblastoma binding protein 1 Antibody [NBP1-26616] - Detection of human ARID4A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID4A antibody NBP1-26616 used for IP at 3 ug per reaction. ARID4A was also immunoprecipitated by another rabbit anti-ARID4A antibody. For blotting immunoprecipitated ARID4A, NBP1-26616 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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Western Blot: EXOSC3 Antibody [NBP1-91874] - Analysis in control (vector only transfected HEK293T lysate) and EXOSC3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: EXOSC3 Antibody [NBP1-91874] - Staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: EXOSC3 Antibody [NBP1-91874] - Staining of human tonsil shows moderate to strong nuclear positivity in non-germinal and germinal center cells.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
RanBP1 Antibody
NB100-79814
RanBP1 Antibody
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP

Publications for RanBP1 Antibody (NB100-79814)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


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Reviews for RanBP1 Antibody (NB100-79814) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-79814:
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(1)
IHC-P
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Images Ratings Applications Species Date Details
Immunofluorescence RanBP1 NB100-79814
Enlarge 		5
reviewed by:
Anonymous
 IF Human 04/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedHEK293
SpeciesHuman
LotA-2

Blocking

Blocking Details3% BSA in PBS; 1h at room temperature

Primary Anitbody

Dilution RatioDilution: 1:400; Buffer: 3% BSA in PBS; 1h at room temperature

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG AlexaFluor 633
Secondary Manufacturer Cat#A-21070
Secondary Concentration4 ug/mL

Details

Detection NotesConfocal microscopy; Zeiss LSM 710 inverted confocal laser scanning microsope
Fixation DetailsFixation: 4% Paraformaldehyde in PBS 15 min; Permeabilization: 0,1% Triton X-100 in PBS 5 min
Wash Description3 x 5 min with PBS
Immunohistochemistry-Paraffin RanBP1 NB100-79814
Enlarge 		5
reviewed by:
Maja Stalekar
 IHC-P Mouse 10/09/2013
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse spinal cord
SpeciesMouse
LotA-2
Commentsheat induced epitope retrieval with Tris-EDTA, pH 9, 20 min. Permeabilization: 0,2% triton X-100 in PBS, 45 min

Blocking

Blocking Details3% BSA in PBS, 1 h, RT

Primary Anitbody

Dilution Ratio1:800 in blocking solution, overnight, 4C

Secondary Antibody

Secondary Descriptiongoat anti-rabbit Alexa Fluor 555
Secondary Manufacturer Cat#Invitrogen, A21428
Secondary Concentration1:1000

Details

Detection Notesconfocal microscopy
Fixation Details4% paraformaldehyde perfusion and postfixation in 10% phosphate-buffered formalin, paraffin embedding, rehydration, antigen retr
Wash DescriptionPBS, 3x5 min

Comments

Commentsheat induced epitope retrieval with Tris-EDTA, pH 9, 20 min. Permeabilization: 0,2% triton X-100 in PBS, 45 min

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RanBP1 Antibody (NB100-79814) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

RanBP1 Overexpression Lysate
RanBP1 Knockout 293T Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-79814AF350
Alexa Fluor 405 NB100-79814AF405
Alexa Fluor 488 NB100-79814AF488
Alexa Fluor 532 NB100-79814AF532
Alexa Fluor 594 NB100-79814AF594
Alexa Fluor 647 NB100-79814AF647
Alexa Fluor 700 NB100-79814AF700
Alexa Fluor 750 NB100-79814AF750
Biotin NB100-79814B
DyLight 350 NB100-79814UV
DyLight 405 NB100-79814V
DyLight 488 NB100-79814G
DyLight 550 NB100-79814R
DyLight 594 NB100-79814DL594
DyLight 650 NB100-79814C
DyLight 680 NB100-79814FR
DyLight 755 NB100-79814IR
FITC NB100-79814F
HRP NB100-79814H
Janelia Fluor 549 NB100-79814JF549
Janelia Fluor 646 NB100-79814JF646
Learn about Custom Antibody Labeling

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Recent Reviews

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5
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1
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Immunofluorescence RanBP1 NB100-79814
Enlarge
 Anonymous
04/16/2015
Application: IF
Species: Human
read this review
Immunohistochemistry-Paraffin RanBP1 NB100-79814
Enlarge
 Maja Stalekar
10/09/2013
Application: IHC-P
Species: Mouse
read this review

Bioinformatics

Gene Symbol RANBP1
Entrez
Uniprot