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RanBP1 Antibody

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Western Blot: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP1 antibody NB100-79814 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Biological Strategies: Western Blot: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. ...read more
Independent Antibodies: Western Blot: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 on HeLa whole cell lysate using NB100-79814. RanBP1 was also immunoprecipitated using rabbit anti-RanBP1 antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

  

Biological Strategies

      

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-79814AF350
Alexa Fluor 405
NB100-79814AF405
Alexa Fluor 488
NB100-79814AF488
Alexa Fluor 532
NB100-79814AF532
Alexa Fluor 594
NB100-79814AF594
Alexa Fluor 647
NB100-79814AF647
Alexa Fluor 700
NB100-79814AF700
Alexa Fluor 750
NB100-79814AF750
Biotin
NB100-79814B
DyLight 350
NB100-79814UV
DyLight 405
NB100-79814V
DyLight 488
NB100-79814G
DyLight 550
NB100-79814R
DyLight 594
NB100-79814DL594
DyLight 650
NB100-79814C
DyLight 680
NB100-79814FR
DyLight 755
NB100-79814IR
FITC
NB100-79814F
HRP
NB100-79814H
Janelia Fluor 549
NB100-79814JF549
Janelia Fluor 646
NB100-79814JF646
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RanBP1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human RAN Binding Protein 1 using the numbering given in entry NP_002873.1 (GeneID 5902).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RANBP1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:2000
  • Immunohistochemistry-Paraffin 1:100-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:1000-1:5000
Application Notes
IHC-P, IHC application reported in customer review.
Control
RanBP1 Overexpression Lysate
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-79814 in the following applications:
Publications
Read Publications using
NB100-79814 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28821644).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RanBP1 Antibody

  • HTF9A
  • MGC88701
  • RAN binding protein 1
  • Ran-binding protein 1
  • RanBP1
  • ran-specific GTPase-activating protein

Background

Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ELISA: Ran Antibody (8D1A6) [NBP2-61832] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Flow Cytometry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of Hela cells using RAN mouse mAb (green) and negative control (red).
Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of GC-7901 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
RCC1 Antibody
NBP1-85637
Immunocytochemistry/Immunofluorescence: RCC1 Antibody [NBP1-85637] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear membrane. Antibody staining is shown in green.
Western Blot: RCC1 Antibody [NBP1-85637] - Analysis using Anti-RCC1 antibody NBP1-85637 (A) shows similar pattern to independent antibody NBP1-85638 (B).
Immunohistochemistry-Paraffin: RCC1 Antibody [NBP1-85637] - Staining of human testis shows strong nuclear positivity in spermatocytes and spermatids.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
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NB120-2938
Western Blot: RanBP2 Antibody [NB120-2938] - Effect of hypoxia on the interaction between HIF1alpha and BICD1. Co-immunoprecipitation of HIF1alpha, Dynein IC, Importin alpha3, RanBP2 with IgG and BICD1 antibodies were shown in left panel. Total protein expressions in lysate were shown in right panel. n = 3. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41418-018-0241-1) licensed under a CC-BY licence.
Immunohistochemistry: RanBP2 Antibody [NB120-2938] - Figure 1 illustrates immunohistochemical staining of RanBP2 in bovine retina using NB120-2938. Figure 2 illustrates Western blot detection of RanBP2 from human retinal extracts using NB120-2938.
Western Blot: RanBP2 Antibody [NB120-2938] - Analysis of human retinal extracts.
(2 Publications)
Species: Bv, Hu, Mu
Applications: IHC, IHC-P, WB
CRM1 Antibody
NB100-79802
Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802] - Human lung cancer. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802] - Mouse teratoma. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunocytochemistry/Immunofluorescence: CRM1 Antibody [NB100-79802] - Association between Nup98-HoxA9 and CRM1 is critical for the Hox Gene activation mediated by Nup98-HoxA9. Top panel: Nup98-HoxA9 interacts and sequesters CRM1 onto Nup98-HoxA9 dots. HeLa cells were transfected with the EGFP-Nup98-HoxA9 expressing plasmid. After 24 hr, cells were fixed and stained with an anti-CRM1 antibody. Arrows indicate the cells transfected. Bottom panel: Nup98-HoxA9 ES cells were fixed and co-stained with anti-FLAG (M2) and anti-CRM1 antibodies. Merged image of FLAG (green) and CRM1 (red) is shown. Bar, 5um. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/09540) licensed under a CC-BY licence.
(11 Publications)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, IP, Simple Western, WB
RanGAP1 Antibody
(OTI1B4)
NBP2-02623
Flow Cytometry: RanGAP1 Antibody (1B4) [NBP2-02623] - Analysis of Hela cells, using anti-RanGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: RanGAP1 Antibody (1B4) [NBP2-02623] - Analysis of Jurkat cells, using anti-RanGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: RanGAP1 Antibody (1B4) [NBP2-02623] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RanGAP1 antibody, and then analyzed by flow cytometry.
Species: Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
SOS2 Antibody
[Unconjugated]
AF6260
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and 293T human embryonic kidney cell line. PVDF Membrane was probed with 2 µg/mL of Goat Anti-Human SOS2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6260) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SOS2 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
Applications: WB
SOS1 Antibody
(SOS-01) - BSA Free
NB110-68800
Western Blot: SOS1 Antibody (SOS-01) [NB110-68800] - Analysis (reducing conditions) of human Sos using anti-human Sos (SOS-1). Lane 1: K562 human Caucasian chronic myeloid leukemia cell line Lane 2: RAJI human Burkitt lymphoma cell line
Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in human primary fibroblasts using anti-Sos (SOS-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Immunocytochemistry/Immunofluorescence: SOS1 Antibody (SOS-01) [NB110-68800] - Staining of Sos in murine transformed fibroblasts using anti-Sos (SOS-01; red). Actin cytoskeleton was decorated by phalloidin (green) and cell nuclei stained with DAPI (blue).
Species: Hu, Mu
Applications: ICC/IF, WB
RASGRF1 Antibody
NBP1-85727
Immunohistochemistry-Paraffin: RASGRF1 Antibody [NBP1-85727] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunocytochemistry/Immunofluorescence: RASGRF1 Antibody [NBP1-85727] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & the Golgi apparatus. Antibody staining is shown in green.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Nestin Antibody
(196908)
MAB1259
Nestin was detected in immersion fixed human fetal neural progenitor cells using 10 µg/mL Human Nestin Monoclonal Antibody (Catalog # MAB1259) for 3 hours at room temperature. Cells were stained (green) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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(3 Reviews)
(97 Publications)
Species: Hu
Applications: CyTOF-reported, ICC, ICFlow
Niemann-Pick C1
Antibody - BSA Free
NB400-148
Knockout Validated: Niemann-Pick C1 Antibody [NB400-148] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NPC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human NPC1 Polyclonal Antibody (Catalog # NB400-148) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NPC1 at approximately 240-260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.
Western Blot: Niemann-Pick C1 Antibody [NB400-148] - Protein expression analysis of normal and NPC-deficient cells after HIV-1 infection. Cells were uninfected or infected with VSVG-HIV-1 and harvested 96 h post-infection. NPC2, NPC1, and beta-actin protein expression was detected via Western blotting in uninfected and infected cells. Gag expression was also detected in the infected cells. Image collected and cropped by CiteAb from the following publication (http://virologyj.biomedcentral.com/articles/10.1186/1743-422X-9-31), licensed under a CC-BY licence.
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(47 Publications)
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NBP1-83213
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Immunohistochemistry-Paraffin: AGFG2 Antibody [NBP1-83213] - Staining of human skeletal muscle shows low expression as expected.
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Species: Hu
Applications: IHC, IHC-P, WB
NTF2 Antibody
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Immunohistochemistry: p120-catenin Antibody (2E8) [NBP2-45646] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: p120-catenin Antibody (2E8) [NBP2-45646] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: p120-catenin Antibody (2E8) [NBP2-45646] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Species: Ca, Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NUP214 Antibody
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Western Blot: NUP214 Antibody [NB100-2244] - Whole cell lysate from HeLa (5, 15 and 50 ug), and HEK293T (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUP214 antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30seconds.
Immunohistochemistry-Paraffin: NUP214 Antibody [NB100-2244] - Section of ovarian carcinoma. Antibody: Affinity purified rabbit anti- NUP214 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: NUP214 Antibody [NB100-2244] - Section of ovarian carcinoma. Antibody: Affinity purified rabbit anti- NUP214 used at a dilution of 1:200 (1ug/ml). Detection: DAB
(1 Publication)
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Applications: IHC, IHC-P, IP, WB
S100A10 Antibody
[Unconjugated]
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Western blot shows lysates of mouse lung and MEF mouse embryonic feeder cells. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100A10 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
S100A10 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Simple Western lane view shows lysates of mouse lung tissue and MEF mouse embryonic feeder cells, loaded at 0.2 mg/mL. A specific band was detected for S100A10 at approximately 11 kDa (as indicated) using 1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
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Applications: IHC, Simple Western, WB
Retinoblastoma
binding protein 1 Ant ...
NBP1-26616
Western Blot: Retinoblastoma binding protein 1 Antibody [NBP1-26616] - Detection of human ARID4A by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ARID4A antibody NBP1-26616 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: Retinoblastoma binding protein 1 Antibody [NBP1-26616] - Detection of human ARID4A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ARID4A antibody NBP1-26616 used for IP at 3 ug per reaction. ARID4A was also immunoprecipitated by another rabbit anti-ARID4A antibody. For blotting immunoprecipitated ARID4A, NBP1-26616 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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Applications: IP, WB
EXOSC3 Antibody
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Western Blot: EXOSC3 Antibody [NBP2-22261] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. NBP2-22261 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: EXOSC3 Antibody [NBP2-22261] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22261 used for IP at 6 ug/mg lysate. EXOSC3 was also immunoprecipitated by another rabbit anti-EXOSC3 antibody. For blotting immunoprecipitated EXOSC3, NBP2-22261 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
(1 Review)
(2 Publications)
Species: Ch, Hu, Mu
Applications: IP, WB
RanBP1 Antibody
NB100-79814
RanBP1 Antibody
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP

Publications for RanBP1 Antibody (NB100-79814)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


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Reviews for RanBP1 Antibody (NB100-79814) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-79814:
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(1)
IHC-P
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Images Ratings Applications Species Date Details
Immunofluorescence RanBP1 NB100-79814
Enlarge 		5
reviewed by:
Simona Darovic
 IF Human 04/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedHEK293
SpeciesHuman
LotA-2
Immunohistochemistry-Paraffin RanBP1 NB100-79814
Enlarge 		5
reviewed by:
Verified Customer
 IHC-P Mouse 10/09/2013
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse spinal cord
SpeciesMouse
LotA-2

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FAQs for RanBP1 Antibody (NB100-79814) (0)

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Control Lysate(s)

RanBP1 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Immunofluorescence RanBP1 NB100-79814
Enlarge
 Simona Darovic
04/16/2015
Application: IF
Species: Human
read this review
Immunohistochemistry-Paraffin RanBP1 NB100-79814
Enlarge
 Verified Customer
10/09/2013
Application: IHC-P
Species: Mouse
read this review

Bioinformatics

Gene Symbol RANBP1
Entrez
Uniprot