RanBP1 Antibody - BSA Free

Western Blot: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP1 antibody NB100-79814 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 on HeLa whole cell lysate using NB100-79814. RanBP1 was also immunoprecipitated using rabbit anti-RanBP1 antibody BL1968 NB100-79815 at 5 mcg/0.5 mg lysate.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

RanBP1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit RanBP1 Antibody - BSA Free (NB100-79814) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-RanBP1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human RAN Binding Protein 1 using the numbering given in entry NP_002873.1 (GeneID 5902).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RANBP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:2000
  • Immunohistochemistry-Paraffin 1:100-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:1000-1:5000
• Application Notes: IHC-P, IHC application reported in customer review.
• Control:

  RanBP1 Overexpression Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28821644).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RanBP1 Antibody - BSA Free

• HTF9A
• MGC88701
• RAN binding protein 1
• Ran-binding protein 1
• RanBP1
• ran-specific GTPase-activating protein

Background

Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RanBP1 Antibody (NB100-79814)(3)

Publications using NB100-79814

• Sugiyama K, Aida T, Nomura M et al. Calpain-dependent degradation of nucleoporins contributes to motor neuron death in a mouse model of chronic excitotoxicity J. Neurosci. 2017-08-16 [PMID: 28821644] (Mouse)
• Yamashita T, Aizawa H, Teramoto S et al. Calpain-dependent disruption of nucleo-cytoplasmic transport in ALS motor neurons Sci Rep 2017-01-03 [PMID: 28045133] (Human)
• Stalekar M, Yin X, Rebolj K et al. Proteomic analyses reveal that loss of TDP-43 affects RNA processing and intracellular transport Neuroscience 2015-05-07 [PMID: 25743254] (WB, Human)

Reviews for RanBP1 Antibody (NB100-79814) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Mouse.

reviewed by: Verified Customer IF Human 04/16/2015

Summary

• Application: Immunofluorescence
• Sample Tested: HEK293
• Species: Human
• Lot: A-2

reviewed by: Maja Stalekar IHC-P Mouse 10/09/2013

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: mouse spinal cord
• Species: Mouse
• Lot: A-2

Bioinformatics

• Gene Symbol: RANBP1
• Entrez:
  • 5902(Human)
  • 19385(Mouse)
• Uniprot:
  • NP_002873.1(Human)