RanBP1 Antibody

Images

 
Biological Strategies: Western Blot: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. ...read more
Independent Antibodies: Western Blot: RanBP1 Antibody [NB100-79814] - Detection of Human RanBP1 on HeLa whole cell lysate using NB100-79814. RanBP1 was also immunoprecipitated using rabbit anti-RanBP1 antibody ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

RanBP1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human RAN Binding Protein 1 using the numbering given in entry NP_002873.1 (GeneID 5902).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RANBP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • Immunohistochemistry 1:100-1:2000
  • Immunohistochemistry-Paraffin 1:100-1:2000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
IHC-P, IHC application reported in customer review.
Control
RanBP1 Overexpression Lysate
RanBP1 Knockout 293T Cell Lysate
Reviewed Applications
Read 2 Reviews rated 5
using
NB100-79814 in the following applications:

Publications
Read Publications using
NB100-79814 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28821644).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for RanBP1 Antibody

  • HTF9A
  • MGC88701
  • RAN binding protein 1
  • Ran-binding protein 1
  • RanBP1
  • ran-specific GTPase-activating protein

Background

Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92% similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for RanBP1 Antibody (NB100-79814)(3)

Reviews for RanBP1 Antibody (NB100-79814) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.

Reviews using NB100-79814:
Filter by Applications
IF
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence RanBP1 NB100-79814
Enlarge
5
reviewed by:
Anonymous
IF Human 04/16/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedHEK293
SpeciesHuman
LotA-2

Blocking

Blocking Details3% BSA in PBS; 1h at room temperature

Primary Anitbody

Dilution RatioDilution: 1:400; Buffer: 3% BSA in PBS; 1h at room temperature

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG AlexaFluor 633
Secondary Manufacturer Cat#A-21070
Secondary Concentration4 ug/mL

Details

Detection NotesConfocal microscopy; Zeiss LSM 710 inverted confocal laser scanning microsope
Fixation DetailsFixation: 4% Paraformaldehyde in PBS 15 min; Permeabilization: 0,1% Triton X-100 in PBS 5 min
Wash Description3 x 5 min with PBS
Immunohistochemistry-Paraffin RanBP1 NB100-79814
Enlarge
5
reviewed by:
Maja Stalekar
IHC-P Mouse 10/09/2013
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample Testedmouse spinal cord
SpeciesMouse
LotA-2
Commentsheat induced epitope retrieval with Tris-EDTA, pH 9, 20 min. Permeabilization: 0,2% triton X-100 in PBS, 45 min

Blocking

Blocking Details3% BSA in PBS, 1 h, RT

Primary Anitbody

Dilution Ratio1:800 in blocking solution, overnight, 4C

Secondary Antibody

Secondary Descriptiongoat anti-rabbit Alexa Fluor 555
Secondary Manufacturer Cat#Invitrogen, A21428
Secondary Concentration1:1000

Details

Detection Notesconfocal microscopy
Fixation Details4% paraformaldehyde perfusion and postfixation in 10% phosphate-buffered formalin, paraffin embedding, rehydration, antigen retr
Wash DescriptionPBS, 3x5 min

Comments

Commentsheat induced epitope retrieval with Tris-EDTA, pH 9, 20 min. Permeabilization: 0,2% triton X-100 in PBS, 45 min

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for RanBP1 Antibody (NB100-79814) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-79814AF350
Alexa Fluor 405 NB100-79814AF405
Alexa Fluor 488 NB100-79814AF488
Alexa Fluor 532 NB100-79814AF532
Alexa Fluor 594 NB100-79814AF594
Alexa Fluor 647 NB100-79814AF647
Alexa Fluor 700 NB100-79814AF700
Alexa Fluor 750 NB100-79814AF750
Biotin NB100-79814B
DyLight 350 NB100-79814UV
DyLight 405 NB100-79814V
DyLight 488 NB100-79814G
DyLight 550 NB100-79814R
DyLight 594 NB100-79814DL594
DyLight 650 NB100-79814C
DyLight 680 NB100-79814FR
DyLight 755 NB100-79814IR
FITC NB100-79814F
HRP NB100-79814H
Janelia Fluor 549 NB100-79814JF549
Janelia Fluor 646 NB100-79814JF646

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Contact Information

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
04/16/2015
Application: IF
Species: Human

Maja Stalekar
10/09/2013
Application: IHC-P
Species: Mouse

Bioinformatics

Gene Symbol RANBP1
Entrez
Uniprot