NUP214 Antibody

Western Blot: NUP214 Antibody [NB100-2244] - Whole cell lysate from HeLa (5, 15 and 50 ug), and HEK293T (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUP214 antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30seconds.

Immunohistochemistry-Paraffin: NUP214 Antibody [NB100-2244] - Section of ovarian carcinoma. Antibody: Affinity purified rabbit anti- NUP214 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Immunohistochemistry-Paraffin: NUP214 Antibody [NB100-2244] - Section of ovarian carcinoma. Antibody: Affinity purified rabbit anti- NUP214 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Immunoprecipitation: NUP214 Antibody [NB100-2244] - Detection of human NUP214 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NUP214 antibody NB100-2244 (lot 2) used for IP at 6 ug per reaction. NUP214 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-NUP214 antibody NB100-2245. For blotting immunoprecipitated NUP214, NB100-2244 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

NUP214 Antibody Summary

• Description: Novus Biologicals Rabbit NUP214 Antibody (NB100-2244) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-NUP214 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1250 and 1300 of human Nuceloporin 214 using the numbering given in entry NP_005076.3 (GeneID 8021).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NUP214
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100- 1:500
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NUP214 Antibody

• 214 kDa nucleoporin
• CAINputative oncogene
• D9S46Enuclear pore complex protein Nup214
• KIAA0023
• MGC104525
• N214
• nucleoporin 214kD (CAIN)
• nucleoporin 214kDa
• Nucleoporin Nup214
• p250
• Protein CAN

Background

The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This gene is a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for proper cell cycle progression and nucleocytoplasmic transport. The 3' portion of this gene forms a fusion gene with the DEK gene on chromosome 6 in a t(6,9) translocation associated with acute myeloid leukemia and myelodysplastic syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NUP214 Antibody (NB100-2244)(1)

Publication using NB100-2244

• Yu SH, Boyce M, Wands AM et al. Metabolic labeling enables selective photocrosslinking of O-GlcNAc-modified proteins to their binding partners. Proc Natl Acad Sci U S A 2012-03-01 [PMID: 22411826]

Bioinformatics

• Gene Symbol: NUP214
• Entrez:
  • 8021(Human)
• Uniprot:
  • NP_005076.3(Human)