Home » G protein alpha 12 » G protein alpha 12 Research Products » G protein alpha 12 Antibody

G protein alpha 12 Antibody

 Submit a Review

Images

 
(
Enlarge)
Immunohistochemistry-Paraffin: G protein alpha 12 Antibody [NBP3-03329] - Analysis of extracts of various cell lines, using G protein alpha 12 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry-Paraffin: G protein alpha 12 Antibody [NBP3-03329] - Analysis of extracts of various cell lines, using G protein alpha 12 antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

100% Guarantee on every product

G protein alpha 12 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human G protein alpha 12 (NP_031379.2).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GNA12
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Immunohistochemistry 1:50-1:200

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for G protein alpha 12 Antibody

  • G alpha-12
  • gep
  • G-protein subunit alpha-12
  • guanine nucleotide binding protein (G protein) alpha 12
  • guanine nucleotide-binding protein subunit alpha-12
  • MGC104623
  • MGC99644
  • NNX3
  • RMP
  • WUGSC:H_GS165O14.2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Somatostatin
Antibody (7G5)
NBP2-37447
Western Blot: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis using SST mAb against human SST recombinant protein. (Expected MW is 38.2 kDa)
Immunohistochemistry-Paraffin: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis of lung cancer tissues using SST mouse mAb with DAB staining.
Flow Cytometry: Somatostatin Antibody (7G5) [NBP2-37447] - Analysis of HepG2 cells using SST mouse mAb (green) and negative control (red).
(1 Review)
(1 Publication)
Species: Hu
Applications: WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready, IF
CRM1 Antibody
NB100-79802
Western Blot: CRM1 Antibody [NB100-79802] - Detection of Human and Mouse CRM1 by Western Blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-CRM1 antibody NB100-79802 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunocytochemistry/Immunofluorescence: CRM1 Antibody [NB100-79802] - Association between Nup98-HoxA9 and CRM1 is critical for the Hox Gene activation mediated by Nup98-HoxA9. (B) The effect of LMB treatment on the cellular localization of Nup98-HoxA9. Nup98-HoxA9 ES cells were cultured either in the presence or absence of 5 nM LMB for 2 hr, fixed and stained with antibodies against FLAG (M2) and CRM1. Merged images of FLAG (green) and CRM1 (red) are shown. Nuclei were stained with DAPI. Bar, 10um. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/09540) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802] - Mouse teratoma. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
(9 Publications)
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
Embigin homolog
Antibody
NBP2-24689
Western Blot: Embigin homolog Antibody [NBP2-24689] - Analysis of Embigin in MCF7 lysate in the 1) absence and 2) presence of immunizing peptide and 3) RAW lysate using Embigin antibody at 0.25 ug/ml.
Species: Hu, Mu
Applications: WB
Progranulin/PGRN
Antibody [Unconjugate ...
AF2557
Western blot shows lysates of mouse uterus tissue and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Progranulin/PGRN at approximately 85‑90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Progranulin/PGRN was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Progranulin was detected in immersion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Progranulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
(3 Reviews)
(21 Publications)
Species: Mu
Applications: WB, IHC, ICC
p19ARF/CDKN2A
Antibody
NB200-106
Western Blot: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of p16INK4a/CDKN2A in (A) NIH/3T3 and (B) MEF cells.
Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody [NB200-106] - Antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
Immunohistochemistry: p19ARF/CDKN2A Antibody [NB200-106] - Adult mouse kidney (glomerulus). Image from verified customer review.
(1 Review)
(42 Publications)
Species: Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
Aminopeptidase
A/ENPEP Antibody (6C3
NBP1-43281
Flow Cytometry: Aminopeptidase A/ENPEP Antibody (6C3) [NBP1-43281] - Detection of Aminopeptidase A/ENPEP in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with PE-conjugated Rat Anti-Mouse CD11b (Catalog # FAB1124P) and Rat Anti-Mouse Aminopeptidase A/ENPEP Monoclonal Antibody (Catalog # NBP1-43281, top panel) or isotype control antibody (Catalog # MAB006, bottom panel), followed by APC-conjugated Goat Anti-Rat IgG (Catalog # F0113).
Flow Cytometry: Aminopeptidase A/ENPEP Antibody (6C3) [NBP1-43281] - Staining of BALB/c bone marrow cells with 0.5 ug of Rat IgG2a K Isotype Control Purified (open histogram) or 0.5 ug of Anti-Mouse BP-1 Purified (filled histogram) followed by Anti-Rat IgG Biotin and Streptavidin PE. Cells in the lymphocyte gate were used
Species: Mu
Applications: Flow, IHC, IHC-P, IP
Catalase Antibody
NBP2-24916
Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.
Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.
(1 Review)
(6 Publications)
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
Insulin Antibody
(E2-E3 (same as INS04 ...
NBP2-34260
Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)
(5 Reviews)
(1 Publication)
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Pancreatic
Polypeptide/PP Antibo
NB100-1793
Immunohistochemistry-Paraffin: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Staining of paraffin embedded Human Intestine with antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunohistochemistry-Frozen: Pancreatic Polypeptide/PP Antibody [NB100-1793] - Analysis of Pancreatic Polypeptide in mouse adult pancreas tissue. IHC-Fr image submitted by a verified customer review.
(3 Reviews)
(14 Publications)
Species: Hu, Mu, Rt, Pm
Applications: Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA
VEGF [HRP]
DVE00
(26 Reviews)
(514 Publications)
Species: Hu
ARF1 Antibody (1D9)
NB300-505
Western Blot: ARF1 Antibody (1D9) [NB300-505] - Analysis of canine heart extract.
Immunocytochemistry/Immunofluorescence: ARF1 Antibody (1D9) [NB300-505] - Analysis of ADP-Ribosylation Factor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.
Immunohistochemistry-Paraffin: ARF1 Antibody (1D9) [NB300-505] - Both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues.
(13 Publications)
Species: Hu, Mu, Rt, Bv, Ca
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
Chromogranin A
Antibody
NB120-15160
Immunohistochemistry-Paraffin: Chromogranin A Antibody [NB120-15160] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse pancreas using Chromogranin A antibody at a dilution of 1:500. The signal was developed using HRP-conjugated anti-rabbit secondary and DAB reagent which followed counterstaining of the section with hematoxylin. The antibody generated a strong staining in Islets of Langerhans. Also, in the periphery of the cells, the pattern of staining was punctate representing the vesicles with Chromogranin A. Cells of lobules and acini showed a weak cytoplasmic and nuclear staining which is potentially the secreted pool of Chromogranin A.
Immunohistochemistry: Chromogranin A Antibody [NB120-15160] - IHC-P analysis fo human pancreas tissue showing specific staining of Chromogranin A in the Islets of Langerhans (dark brown staining).
Immunocytochemistry/Immunofluorescence: Chromogranin A Antibody [NB120-15160] - Neuro2a cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Chromogranin A Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
(2 Reviews)
(18 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
ABCA4 Antibody
(3F4)
NBP1-30032
Western Blot: ABCA4 Antibody (3F4) [NBP1-30032] - Characterization of ABCA4 mRNA expression and western blot analyses of ABCA4 protein levels in the canine retina. Western blot analyses of ABCA4 (above), GAPDH (middle), and RHO (below) protein levels in retinal tissue of dogs with the three different genotypes. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pgen.1007873), licensed under a CC-BY licence.
Immunohistochemistry-Frozen: ABCA4 Antibody (3F4) [NBP1-30032] - IHC-Fr of normal feline retina with antibodies ABCA4 3F4 (red) and HCAR (green). ABCA4 antibody (1:1000) labels outer segments of photoreceptors and HCAR antibody labels cones. IHC-Fr image submitted by a verified customer review.
Immunohistochemistry: ABCA4 Antibody (3F4) [NBP1-30032] - staining of adult mouse retina showing specific immunolabeling of the ABCA4 protein. Photo courtesy of Mary Raven, University of California, Santa Barbara, CA.
(1 Review)
(5 Publications)
Species: Hu, Mu, Bv, Ca, Fe, Xp
Applications: WB, ICC/IF, IHC, IHC-Fr
Inhibin alpha
Antibody
NBP2-26096
Immunohistochemistry-Paraffin: Inhibin alpha Antibody [NBP2-26096] - 60X image of Hu Testis stained with Inhibin alpha antibody at 1:50 shows questionable punctate or vesicular staining, both cytoplasmic and somewhat nuclear, in fluorescent green 488. Did not correspond completely with multiple other INHA antibodies tested. IHC staining with citrate pH 6 antigen retrieval; detected staining with a 488-conjugated anti-goat secondary antibody. IHC image submitted by a verified customer review.
(1 Review)
(1 Publication)
Species: Hu, Bv
Applications: WB, IHC, IHC-P, PEP-ELISA
Carbonic Anhydrase
I/CA1 Antibody
NBP1-88191
Western Blot: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Comparative proteome analysis of 3xTg-AD and control samples at different stages of AD. Immunoblot analysis of most regulated hits. Soluble fractions of brain proteins were analyzed from four 2-month-old control and 3xTg-AD mice animals, respectively. Hebp1 and Glo1 levels were consistently elevated in the transgenic animals as compared to wild type controls. Ca1 levels were reduced in transgenic animals. Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/47498), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Staining of human rectum shows strong cytoplasmic and nuclear positivity in glandular cells.
Western Blot: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Analysis in human cell line HELA.
(3 Publications)
Species: Hu, Rt
Applications: WB, IHC, IHC-P
Recombinant Human
Gastrin GST (N-Term) ...
H00002520-P01
SDS-Page: Recombinant Human Gastrin peptide Protein [H00002520-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.
(2 Publications)
Species: Hu
URI Antibody
NBP1-79413
Western Blot: URI Antibody [NBP1-79413] - MCF7, Antibody Dilution: 1.0 ug/ml There is BioGPS gene expression data showing that URI1 is expressed in MCF7.
Western Blot: URI Antibody [NBP1-79413] - Human Muscle lysate, concentration 0.2-1 ug/ml.
Western Blot: URI Antibody [NBP1-79413] - Hela, Antibody Dilution: 1.0 ug/ml There is BioGPS gene expression data showing that URI1 is expressed in HeLa.
Species: Hu
Applications: WB
ARF6 Antibody
NBP1-58310
Western Blot: ARF6 Antibody [NBP1-58310] - Titration: 0.2-1ug/ml, Positive Control: MCF7 cell Lysate
Immunohistochemistry-Paraffin: ARF6 Antibody [NBP1-58310] - Human Brain Tissue (Cerebellum), antibody concentration 5 ug/ml.
Western Blot: ARF6 Antibody [NBP1-58310] - Titration: 2 ug/ml Positive Control: Human NT-2 cells and Mouse WT brain.
Species: Hu, Mu
Applications: WB, IHC, IHC-P
Cytohesin-1
Antibody (6G11)
NBP2-22513
Immunocytochemistry/Immunofluorescence: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Analysis of Cytohesin 1,2 in NIH-3T3 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cytohesin 1,2 monoclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 1,2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.
Immunohistochemistry: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Normal biopsies of deparaffinized human placenta tissue.
Immunocytochemistry/Immunofluorescence: Cytohesin-1 Antibody (6G11) [NBP2-22513] - Analysis of Cytohesin 1,2 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Cytohesin 1,2 monoclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Cytohesin 1,2 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, IP
G protein alpha 12
Antibody
NBP3-03329
G protein alpha 12 Antibody
Species: Mu, Rt
Applications: WB, IHC

Publications for G protein alpha 12 Antibody (NBP3-03329) (0)

There are no publications for G protein alpha 12 Antibody (NBP3-03329).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for G protein alpha 12 Antibody (NBP3-03329) (0)

There are no reviews for G protein alpha 12 Antibody (NBP3-03329). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for G protein alpha 12 Antibody (NBP3-03329) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

Additional G protein alpha 12 Products

Bioinformatics Tool for G protein alpha 12 Antibody (NBP3-03329)

Discover related pathways, diseases and genes to G protein alpha 12 Antibody (NBP3-03329). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for G protein alpha 12 Antibody (NBP3-03329)

Discover more about diseases related to G protein alpha 12 Antibody (NBP3-03329).
 

Pathways for G protein alpha 12 Antibody (NBP3-03329)

View related products by pathway.

PTMs for G protein alpha 12 Antibody (NBP3-03329)

Learn more about PTMs related to G protein alpha 12 Antibody (NBP3-03329).
 

Research Areas for G protein alpha 12 Antibody (NBP3-03329)

Find related products by research area.

Blogs on G protein alpha 12

There are no specific blogs for G protein alpha 12, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our G protein alpha 12 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol GNA12