Glyoxalase I Antibody (Glo1a)

Images

 
Western Blot: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - HeLa whole cell extracts.
Immunocytochemistry/ Immunofluorescence: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Immunostaining of human retinal endothelial cells. Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells ...read more
Simple Western: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Simple Western lane view shows a specific band for GLO1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the ...read more
Simple Western: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Simple Western lane view shows a specific band detected for GLO1 in HeLa lysate. This experiment was performed under reducing conditions using the Wes or ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ELISA, ICC/IF, IP
Clone
Glo1a
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Glyoxalase I Antibody (Glo1a) Summary

Immunogen
Full length human GLO1 protein [Swiss-Prot #Q04760].
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GLO1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1.0 - 2.0 ug/ml
  • Simple Western 1:50
  • ELISA 0.15 ng / 1 ug protein
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Immunoprecipitation
Application Notes
This GLO1 antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen approx. 21 kDa. Immunoprecipitation was reported in scientific literature.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Glyoxalase I Lysate (NBL1-11117)
HeLa Lysate (NB800-PC1)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-19015 in the following applications:

Publications
Read Publications using
NBP1-19015 in the following applications:

  • IP
    1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Glyoxalase I Antibody (Glo1a)

  • Aldoketomutase
  • EC 4.4.1.5
  • GLO1
  • GLOD1
  • Glx I
  • GLYI
  • glyoxalase domain containing 1
  • Glyoxalase I
  • glyoxalase Ialdoketomutase
  • Ketone-aldehyde mutase
  • lactoyl glutathione lyase
  • lactoylglutathione lyase
  • Methylglyoxalase
  • S-D-lactoylglutathione methylglyoxal lyase

Background

Glyoxalase 1 (GLO1) is the main enzyme in glyoxalase system, a metabolic pathway responsible for detoxifying alpha-oxoaldehydes, particularly methylglyoxal; and is known for converting reactive dicarbonyls into non-toxic intermediates ultimately protecting living cells from producing and accumulating advanced glycation end products, AGEs. GLO1 has also been studied with reference to anxiety, and a murine model showed that this gene's overexpression increases anxiety-related behavior by reducing the GABAA receptor agonist MG. Moreover, an association analysis in a human population with anxiety disorder subtypes suggested a role for GLO1's implication in this phenotype. GLO1 plays an important role in tumour growth and partly in chemotherapy survival as evidenced by a number of studies suggesting that some tumours rely on GLO1 to increase their viability and resistance to multiple chemotherapeutic agents. Furthermore, genetic studies have implicated GLO1 polymorphisms in panic disorder and restless legs syndrome (RLS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glyoxalase I Antibody (NBP1-19015)(2)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IP, WB.


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IP
(1)
WB
(2)
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Review for Glyoxalase I Antibody (NBP1-19015) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-19015:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Glyoxalase I NBP1-19015
Enlarge
reviewed by:
Giulia Ribelli
WB Human 03/20/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman osteosarcoma cells – MG63,Human liposarcoma cells- 402-91
SpeciesHuman
LotA-3

Product General Protocols

View specific protocols for Glyoxalase I Antibody (NBP1-19015): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Glyoxalase I Antibody (NBP1-19015) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-19015AF405
Alexa Fluor 488 Labeled NBP1-19015AF488
Alexa Fluor 647 Labeled NBP1-19015AF647
Alexa Fluor 700 Labeled NBP1-19015AF700
Biotin Labeled NBP1-19015B
DyLight 350 Labeled NBP1-19015UV
DyLight 405 Labeled NBP1-19015V
DyLight 488 Labeled NBP1-19015G
DyLight 550 Labeled NBP1-19015R
DyLight 650 Labeled NBP1-19015C
DyLight 680 Labeled NBP1-19015FR
DyLight 755 Labeled NBP1-19015IR
FITC Labeled NBP1-19015F
HRP Labeled NBP1-19015H

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Calculators

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Giulia Ribelli
03/20/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol GLO1
Entrez