Glyoxalase I Antibody (Glo1a)

Images

 
Western Blot: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Detection of GLO-1 in human cell lines. Lane 1,2 and 3: MG-63 osteosarcoma cell line (positive control), 402-91 liposarcoma cell line and 402-91 liposarcoma ...read more
Immunocytochemistry/ Immunofluorescence: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Immunostaining of human retinal endothelial cells. Immunoreactivity for hGlx-1 was detected in human retinal endothelial cells ...read more
Western Blot: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - HeLa whole cell extracts.
Simple Western: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Simple Western lane view shows a specific band for GLO1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the ...read more
Simple Western: Glyoxalase I Antibody (Glo1a) [NBP1-19015] - Simple Western lane view shows a specific band detected for GLO1 in HeLa lysate. This experiment was performed under reducing conditions using the Wes or ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ELISA, ICC/IF, IHC, IP
Clone
Glo1a
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Glyoxalase I Antibody (Glo1a) Summary

Immunogen
Full length human GLO1 protein [Swiss-Prot #Q04760].
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
GLO1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 0.15 ng / 1 ug protein
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry-Paraffin reported in scientific literature (10.3892/ol.2021.12808)
  • Immunoprecipitation reported in scientific literature
  • Simple Western 1:50
  • Western Blot
Application Notes
This GLO1 antibody is useful for ELISA, Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen approx. 21 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Glyoxalase I Overexpression Lysate
HeLa Whole Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
NBP1-19015 in the following applications:

Publications
Read Publications using
NBP1-19015 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    6 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Glyoxalase I Antibody (Glo1a)

  • Aldoketomutase
  • EC 4.4.1.5
  • GLO1
  • GLOD1
  • Glx I
  • GLYI
  • glyoxalase domain containing 1
  • Glyoxalase I
  • glyoxalase Ialdoketomutase
  • Ketone-aldehyde mutase
  • lactoyl glutathione lyase
  • lactoylglutathione lyase
  • Methylglyoxalase
  • S-D-lactoylglutathione methylglyoxal lyase

Background

Glyoxalase 1 (GLO1) is the main enzyme in glyoxalase system, a metabolic pathway responsible for detoxifying alpha-oxoaldehydes, particularly methylglyoxal; and is known for converting reactive dicarbonyls into non-toxic intermediates ultimately protecting living cells from producing and accumulating advanced glycation end products, AGEs. GLO1 has also been studied with reference to anxiety, and a murine model showed that this gene's overexpression increases anxiety-related behavior by reducing the GABAA receptor agonist MG. Moreover, an association analysis in a human population with anxiety disorder subtypes suggested a role for GLO1's implication in this phenotype. GLO1 plays an important role in tumour growth and partly in chemotherapy survival as evidenced by a number of studies suggesting that some tumours rely on GLO1 to increase their viability and resistance to multiple chemotherapeutic agents. Furthermore, genetic studies have implicated GLO1 polymorphisms in panic disorder and restless legs syndrome (RLS).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glyoxalase I Antibody (NBP1-19015)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC-P, IP, WB.


Filter By Application
IHC-P
(1)
IP
(1)
WB
(6)
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Human
(3)
Mouse
(3)
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Showing Publications 1 - 8 of 8.
Publications using NBP1-19015 Applications Species
Inoue M, Nakagawa Y, Azuma M et al. The PKM2 inhibitor shikonin enhances piceatannol-induced apoptosis of glyoxalase I-dependent cancer cells Genes to cells : devoted to molecular & cellular mechanisms 2023-11-14 [PMID: 37963646] (WB, Human) WB Human
Suh EH, Geraldes CFGC, Chirayil S et al. Detection of glucose-derived D- and L-lactate in cancer cells by the use of a chiral NMR shift reagent Cancer & Metabolism 2021-12-01 [PMID: 34742347] (WB) WB
Motomura H, Ozaki A, Tamori S et al. Glyoxalase 1 and protein kinase Clambda as potential therapeutic targets for late stage breast cancer Oncology Letters 2021-05-24 [PMID: 34093768] (IHC-P, Human) IHC-P Human
Punzalan LL, Jiang L, Mao D et al. Chemoproteomic Profiling of a Pharmacophore-Focused Chemical Library Cell Chem Biol 2020-04-28 [PMID: 32402240] (WB, Human) WB Human
Zeng S, Zhang QY, Huang J et al. Opposing roles of RAGE and Myd88 signaling in extensive liver resection. The FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology. 2011-11-10 [PMID: 22075646] (WB, Mouse) WB Mouse
Mailankot M et al. Glyoxalase I activity and immunoreactivity in the aging human lens. Biogerontology. 2009-02-24 [PMID: 19238574] (IP, WB, Mouse) IP, WB Mouse
Halbert D, Domenyuk V, Spetzler D et al. Aptamers and uses thereof United States Patent Application US 9958448 B2 2018-01-01
Luengo A, Abbott KL, Davidson SM, et al. Reactive metabolite production is a targetable liability of glycolytic metabolism in lung cancer Nat Commun 2019-12-06 [PMID: 31811141] (WB, Mouse) WB Mouse

Review for Glyoxalase I Antibody (NBP1-19015) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-19015:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Glyoxalase I NBP1-19015
Enlarge
5
reviewed by:
Giulia Ribelli
WB Human 03/20/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman liposarcoma cells- 402-91,Human osteosarcoma cells – MG63
SpeciesHuman
LotA-3

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Glyoxalase I Antibody (NBP1-19015) (0)

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mFluor Violet Conjugated Antibodies

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Recent Reviews

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Giulia Ribelli
03/20/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol GLO1