FACA/FANCA Antibody

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Western Blot: FACA/FANCA Antibody [NB100-2564] - Detection of FANC A in transfected COS1 cell lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

FACA/FANCA Antibody Summary

Immunogen
A synthetic peptide within residues 1200-1300 of the human FANCA protein. [Swiss-Prot# O15360]
Localization
The major form is nuclear. The minor form is cytoplasmic.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FANCA
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
This FANCA antibody is useful for Western Blot, where a band is seen at approximately 170 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
170 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NB100-2564.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
No Preservative
Purity
Unpurified

Alternate Names for FACA/FANCA Antibody

  • FA
  • FA1
  • FAA
  • FAAMGC75158
  • FACA
  • FACAFanconi anemia group A protein
  • FAH
  • FA-H
  • FANCA
  • FANCH
  • Fanconi anemia, complementation group A
  • Fanconi anemia, complementation group H
  • Fanconi anemia, type 1
  • Protein FACA

Background

FANCA (Fanconi anemia group A protein) is a DNA repair protein which plays a key role in post-replication repair, cell cycle checkpoint function, interstrand DNA cross-link repair and maintenance of chromosome stability. FANCA belongs to multisubunit FA complex (FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM) which is absent in FA patients. When complexed with FANCF, FANCG and FANCL (but not with FANCC, nor FANCE), FANCA interacts with HES1 and this interaction is crucial for the stability as well as nuclear localization of FA core complex proteins. FANCA-FANCC/FANCG complex may include EIF2AK2 and HSP70 also and it directly interacts with FAAP20/C1orf86. Localized mainly in the nucleus and cytoplasm, it gets phosphorylated upon DNA damage through ATM/ATR. FANCA is a most frequently mutated gene in FA (representing 60-70% of the cases) and over 350 unique mutations have been reported which includes large genomic deletions mediated by unusually high density of ALU repetitions found in its genomic sequence. FANCA sequence alterations or its altered expression has been associated with ovarian cancer and leukemia, and homozygous mutations of FANCA have been linked to esophageal cancer. Defects in FANCA are a cause of Fanconi anemia (FA), a rare disorder characterized by physical abnormalities, bone marrow failure (BMF), increased risk of malignancies, and cellular hypersensitivity to DNA cross-linking agents.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol FANCA
Entrez
Uniprot