Complement Factor D/Adipsin Antibody

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Western blot shows lysates of mouse plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Complement Factor D/Adipsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5430) followed by ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Conjugate Size Price
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Complement Factor D/Adipsin Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Complement Factor D/Adipsin
Ile26-Ser259
Accession # P03953
Specificity
Detects mouse Complement Factor D/Adipsin in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Complement Factor D is observed and less than 5% cross-reactivity with rhGranzyme A is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CFD
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF5430 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Factor D/Adipsin Antibody

  • Adipsin
  • ADN
  • ADNcomplement factor D
  • AMBP-1
  • C3 convertase activator
  • CFD
  • complement factor D (adipsin)
  • complement factor D preproprotein
  • Complement Factor D
  • D component of complement (adipsin)
  • DF
  • EC 3.4.21
  • EC 3.4.21.46
  • PFD
  • Properdin factor DADIPSIN

Background

Complement Factor D (also Adipsin, C3 convertase activator and Properdin factor D) is a 37‑43 kDa member of the S1 peptidase family of enzymes. In mouse, it is secreted by fat cells and helps form C3 convertase, a complex of C3b, Factor B and Factor D. This complex cleaves C3 into C3b and C3a, the latter of which is converted into ASP, a molecule that binds the adipocyte receptor C5L2 and induces triglyceride synthesis. Mature mouse Factor D is 234 amino acids (aa) in length (aa 26‑259). In contains one peptidase S1 domain that cleaves an Arg-Lys bond (aa 26‑254). There are at least two potential alternative splice forms. One shows a two aa substitution for aa 1‑71, and a second shows a 15 aa substitution for aa 1‑107. Mature mouse factor D shares 85% and 68% aa identity with rat and human Factor D, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Complement Factor D/Adipsin Antibody (AF5430)(13)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 2 applications: IHC, Western Blot.


Filter By Application
IHC
(1)
Western Blot
(8)
All Applications
Filter By Species
Mouse
(8)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using AF5430 Applications Species
He, Y;Zhang, R;Yu, L;Zahr, T;Li, X;Kim, TW;Qiang, L; PPAR gamma Acetylation in Adipocytes Exacerbates BAT Whitening and Worsens Age-Associated Metabolic Dysfunction Cells 5/18/2023 [PMID: 37408258]
Nicole Aaron, Tarik Zahr, Ying He, Lexiang Yu, Brent Mayfield, Utpal B. Pajvani et al. Acetylation of PPAR gamma in macrophages promotes visceral fat degeneration in obesity Life Metabolism 2023-05-22 [PMID: 37213714]
Wan, Q;Calhoun, C;Zahr, T;Qiang, L; Uncoupling Lipid Synthesis from Adipocyte Development Biomedicines 2023-04-09 [PMID: 37189751] (IHC, Western Blot, Mouse) IHC, Western Blot Mouse
Q Wan, B Huang, T Li, Y Xiao, Y He, W Du, BZ Wang, GF Dakin, M Rosenbaum, MD Goncalves, S Chen, KW Leong, L Qiang Selective targeting of visceral adiposity by polycation nanomedicine Nature nanotechnology, 2022-12-01;0(0):. 2022-12-01 [PMID: 36456644] (Western Blot, Mouse) Western Blot Mouse
Ying He, Alana B'nai Taub, Lexiang Yu, Yifan Yao, Ruotong Zhang, Tarik Zahr et al. PPAR gamma Acetylation Orchestrates Adipose Plasticity and Metabolic Rhythms Advanced Science 2023-01-01 [PMID: 36394167]
Chan Hee J Choi, William Barr, Samir Zaman, Corey Model, Annsea Park, Mascha Koenen et al. LRG1 is an adipokine that promotes insulin sensitivity and suppresses inflammation eLife 2022-11-08 [PMID: 36346018]
M Mizuno, B Khaledian, M Maeda, T Hayashi, S Mizuno, E Munetsuna, T Watanabe, S Kono, S Okada, M Suzuki, S Takao, H Minami, N Asai, F Sugiyama, S Takahashi, Y Shimono Adipsin-Dependent Secretion of Hepatocyte Growth Factor Regulates the Adipocyte-Cancer Stem Cell Interaction Cancers, 2021-08-23;13(16):. 2021-08-23 [PMID: 34439392] (Western Blot, Mouse) Western Blot Mouse
N Aaron, MJ Kraakman, Q Zhou, Q Liu, S Costa, J Yang, L Liu, L Yu, L Wang, Y He, L Fan, H Hirakawa, L Ding, J Lo, W Wang, B Zhao, E Guo, L Sun, CJ Rosen, L Qiang Adipsin promotes bone marrow adiposity by priming mesenchymal stem cells Elife, 2021-06-22;10(0):. 2021-06-22 [PMID: 34155972] (Western Blot, Mouse) Western Blot Mouse
D Pauly, D Agarwal, N Dana, N Schäfer, J Biber, KA Wunderlich, Y Jabri, T Straub, NR Zhang, AK Gautam, BHF Weber, SM Hauck, M Kim, CA Curcio, D Stambolian, M Li, A Grosche Cell-Type-Specific Complement Expression in the Healthy and Diseased Retina Cell Rep, 2019-11-26;29(9):2835-2848.e4. 2019-11-26 [PMID: 31775049] (Western Blot, Mouse) Western Blot Mouse
X Wu, I Hutson, AM Akk, S Mascharak, CTN Pham, DE Hourcade, R Brown, JP Atkinson, CA Harris Contribution of Adipose-Derived Factor D/Adipsin to Complement Alternative Pathway Activation: Lessons from Lipodystrophy J. Immunol., 2018-03-12;0(0):. 2018-03-12 [PMID: 29531168] (Western Blot, Mouse) Western Blot Mouse
Show All 13 Publications.

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Bioinformatics

Gene Symbol CFD
Uniprot