Complement Factor D/Adipsin Antibody

Western blot shows lysates of mouse plasma. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Complement Factor D/Adipsin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5430) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Complement Factor D/Adipsin at approximately 45kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Mu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Complement Factor D/Adipsin Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Complement Factor D/Adipsin
  Ile26-Ser259
  Accession # P03953
• Specificity: Detects mouse Complement Factor D/Adipsin in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Complement Factor D is observed and less than 5% cross-reactivity with rhGranzyme A is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CFD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Immunoprecipitation 25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Factor D/Adipsin Antibody

• Adipsin
• ADN
• ADNcomplement factor D
• AMBP-1
• C3 convertase activator
• CFD
• complement factor D (adipsin)
• complement factor D preproprotein
• Complement Factor D
• D component of complement (adipsin)
• DF
• EC 3.4.21
• EC 3.4.21.46
• PFD
• Properdin factor DADIPSIN

Background

Complement Factor D (also Adipsin, C3 convertase activator and Properdin factor D) is a 37‑43 kDa member of the S1 peptidase family of enzymes. In mouse, it is secreted by fat cells and helps form C3 convertase, a complex of C3b, Factor B and Factor D. This complex cleaves C3 into C3b and C3a, the latter of which is converted into ASP, a molecule that binds the adipocyte receptor C5L2 and induces triglyceride synthesis. Mature mouse Factor D is 234 amino acids (aa) in length (aa 26‑259). In contains one peptidase S1 domain that cleaves an Arg-Lys bond (aa 26‑254). There are at least two potential alternative splice forms. One shows a two aa substitution for aa 1‑71, and a second shows a 15 aa substitution for aa 1‑107. Mature mouse factor D shares 85% and 68% aa identity with rat and human Factor D, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Complement Factor D/Adipsin Antibody (AF5430)(4)

Publications using AF5430

• D Pauly, D Agarwal, N Dana, N Schäfer, J Biber, KA Wunderlich, Y Jabri, T Straub, NR Zhang, AK Gautam, BHF Weber, SM Hauck, M Kim, CA Curcio, D Stambolian, M Li, A Grosche Cell-Type-Specific Complement Expression in the Healthy and Diseased Retina Cell Rep, 2019;29(9):2835-2848.e4. 2019 [PMID: 31775049] (Western Blot, Mouse)
• X Wu, I Hutson, AM Akk, S Mascharak, CTN Pham, DE Hourcade, R Brown, JP Atkinson, CA Harris Contribution of Adipose-Derived Factor D/Adipsin to Complement Alternative Pathway Activation: Lessons from Lipodystrophy J. Immunol., 2018;0(0):. 2018 [PMID: 29531168] (Western Blot, Mouse)
• Regulation of C3 Activation by the Alternative Complement Pathway in the Mouse Retina PLoS ONE, 2016;11(8):e0161898. 2016 [PMID: 27564415] (Western Blot, Mouse)
• Zou, Lin, Feng, Yan, Li, Yan, Zhang, Ming, Chen, Chan, Cai, Jiayan, Gong, Yu, Wang, Larry, Thurman, Joshua M, Wu, Xiaobo, Atkinson, John P, Chao, Wei Complement factor B is the downstream effector of TLRs and plays an important role in a mouse model of severe sepsis. J Immunol, 2013;191(11):5625-35. 2013 [PMID: 24154627] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: CFD
• Uniprot:
  • P03953()