Complement Component C3d Antibody [Biotin]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Complement Component C3d Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse Complement Component C3d
  His1002-Arg1303 (Cys1010Ser)
  Accession # P01027
• Specificity: Detects mouse Complement Component C3d in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse Complement Component C5a is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Component C3d Antibody [Biotin]

• AHUS5;ARMD9;ASP;C3a;C3b;Complement C3;CPAMD1;HEL-S-62p
• Complement Component C3d

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Complement Component C3d Antibody (BAF2655)(9)

Publications using BAF2655

• Salarian M, Ghim M, Toczek J et al. Homeostatic, Non-Canonical Role of Macrophage Elastase in Vascular Integrity Circulation research 2023-01-24 [PMID: 36691905]
  
  Details:
  Mouse plasma
• Heather Kerr, Andrew P. Herbert, Elisavet Makou, Dariusz Abramczyk, Talat H. Malik, Hannah Lomax-Browne et al. Murine Factor H Co-Produced in Yeast With Protein Disulfide Isomerase Ameliorated C3 Dysregulation in Factor H-Deficient Mice Frontiers in Immunology 2021-05-12 [PMID: 34054871]
• Talat M, Daniel G, Deborah L et al. Gain-of-function factor H-related 5 protein impairs glomerular complement regulation resulting in kidney damage. Proc Natl Acad Sci U S A. 2021-03-30 [PMID: 33753502]
• Alyssa C. Gilmore, Yuchun Zhang, H. Terence Cook, Deborah P. Lavin, Suresh Katti, Yi Wang et al. Complement activity is regulated in C3 glomerulopathy by IgG–factor H fusion proteins with and without properdin targeting domains Kidney International 2021-02-01 [PMID: 33129896] (Immunohistochemistry, Mouse)
• Xiaoxu Wang, Menno Van Lookeren Campagne, Kenneth J. Katschke, Damodar Gullipalli, Takashi Miwa, Yoshiyasu Ueda et al. Prevention of Fatal C3 Glomerulopathy by Recombinant Complement Receptor of the Ig Superfamily Journal of the American Society of Nephrology 2018-08-01 [PMID: 29895552]
• Nichols E, Barbour T, Pappworth I, Wong E, Palmer J, Sheerin N, Pickering M, Marchbank K An extended mini-complement factor H molecule ameliorates experimental C3 glomerulopathy. Kidney Int, 2015-07-29;88(6):1314-1322. 2015-07-29 [PMID: 26221753] (IHC, Mouse)
• Xu Z, Qiu Q, Tian J, Smith J, Conenello G, Morita T, Byrnes A Coagulation factor X shields adenovirus type 5 from attack by natural antibodies and complement. Nat Med, 2013-03-24;19(4):452-7. 2013-03-24 [PMID: 23524342] (Western Blot, Mouse)
• Chen M, Forrester JV, Xu H Dysregulation in Retinal Para-Inflammation and Age-Related Retinal Degeneration in CCL2 or CCR2 Deficient Mice. PLoS ONE, 2011-08-05;6(8):e22818. 2011-08-05 [PMID: 21850237] (IHC-Fr, Mouse)
• Leung VW, Yun S, Botto M, Mason JC, Malik TH, Song W, Paixao-Cavalcante D, Pickering MC, Boyle JJ, Haskard DO Decay-accelerating factor suppresses complement C3 activation and retards atherosclerosis in low-density lipoprotein receptor-deficient mice. Am. J. Pathol., 2009-09-03;175(4):1757-67. 2009-09-03 [PMID: 19729477] (ELISA Development, IHC-Fr, Mouse)

Bioinformatics

• Uniprot:
  • P01027()