Complement Component C3d Antibody [Unconjugated]

Complement Component C3d was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse Complement Component C3d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2655) overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to basement membrane. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Complement Component C3d was detected in perfusion fixed paraffin-embedded sections of rat kidney using Goat Anti-Mouse Complement Component C3d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2655) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

The activation of mTORC1 in KCs enhances complement alternative system.a Expression heatmap of genes of neutrophils chemotaxis or complement activation analyzed by RNA-seq from Tsc1+/+ and Tsc1-/- BMMs (n = 3 each). b Western blotting result was shown the expression of CFB protein in hepatic tissues from mice. c Left, representative co-immunofluorescent staining images for F4/80 with CFB. Scale bar = 50 μm. Right, quantitative determination of F4/80+ and CFB+ cells among groups as indicated, n = 3. d Western blotting assay showing the abundance for TSC1, CFB, and p-S6 in BMMs. e qRT-PCR analysis showing the CFB mRNA abundance in BMMs, n = 3. f Western blotting assay showing the abundance for TSC1, CFB, and p-S6 in KCs. g qRT-PCR analysis showing the CFB mRNA abundance in KCs, n = 3. h Representative immunofluorescent staining images for C3d. Scale bar = 50 μm. i Representative immunostaining images for C5b-9. Scale bar = 50 μm. j Western blotting assay showing the abundance for Rheb and CFB in BMMs. k Western blotting assay showing the abundance for Rheb and CFB in KCs. l Representative co-immunofluorescent staining images for F4/80 with CFB (white arrows). Scale bar = 100 μm. m Quantitative determination of F4/80+ and CFB+ cells among groups as indicated, n = 3. n Representative immunofluorescent staining images for C3d. Scale bar = 100 μm. o Representative immunostaining images for C5b-9 among groups as indicated. Scale bar = 100 μm. *p < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36494334), licensed under a CC-BY license. Not internally tested by R&D Systems.

The activation of mTORC1 in KCs enhances complement alternative system.a Expression heatmap of genes of neutrophils chemotaxis or complement activation analyzed by RNA-seq from Tsc1+/+ and Tsc1-/- BMMs (n = 3 each). b Western blotting result was shown the expression of CFB protein in hepatic tissues from mice. c Left, representative co-immunofluorescent staining images for F4/80 with CFB. Scale bar = 50 μm. Right, quantitative determination of F4/80+ and CFB+ cells among groups as indicated, n = 3. d Western blotting assay showing the abundance for TSC1, CFB, and p-S6 in BMMs. e qRT-PCR analysis showing the CFB mRNA abundance in BMMs, n = 3. f Western blotting assay showing the abundance for TSC1, CFB, and p-S6 in KCs. g qRT-PCR analysis showing the CFB mRNA abundance in KCs, n = 3. h Representative immunofluorescent staining images for C3d. Scale bar = 50 μm. i Representative immunostaining images for C5b-9. Scale bar = 50 μm. j Western blotting assay showing the abundance for Rheb and CFB in BMMs. k Western blotting assay showing the abundance for Rheb and CFB in KCs. l Representative co-immunofluorescent staining images for F4/80 with CFB (white arrows). Scale bar = 100 μm. m Quantitative determination of F4/80+ and CFB+ cells among groups as indicated, n = 3. n Representative immunofluorescent staining images for C3d. Scale bar = 100 μm. o Representative immunostaining images for C5b-9 among groups as indicated. Scale bar = 100 μm. *p < 0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36494334), licensed under a CC-BY license. Not internally tested by R&D Systems.

Down regulation of CFB in liver protects against Con-A induced liver injury.a Western blotting assay showing CFB expression in mouse livers after shRNA-CFB injection. b Representative co-immunofluorescent staining images for F4/80 with CFB (white arrows). Scale bar = 100 μm. c The strategy for establishing a mouse model of injection of shRNA-CFB and Con-A administration. d The ALT levels in serum of mice exposed to Con-A for 8 h, n = 6. e Representative HE-stained mouse livers. Scale bar = 100 μm. f Liver sections of were immunofluorescent stained with TUNEL. Scale bar = 200 μm. g Left, representative immunofluorescent staining images for ly6b. Scale bar = 100 μm. Right, quantitative determination of ly6b+ cells among groups as indicated, n = 4. h Left, representative immunofluorescent staining images for C3d. Scale bar = 100 μm. Right, quantitative determination of C3d area in a field of vision, n = 4. i Representative immunostaining images for C5b-9. Scale bar = 100 μm. j Left, representative immunofluorescent staining images for F4/80 (white arrows). Scale bar = 100 μm. Right, quantitative determination of F4/80+ cells among groups as indicated, n = 4. *p < 0.05. k Schematic working model on the role of mTORC1 activation in hepatocytes and KCs in the pathogenesis of ALD. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36494334), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Complement Component C3d Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse Complement Component C3d
  His1002-Arg1303 (Cys1010Ser)
  Accession # P01027
• Specificity: Detects mouse Complement Component C3d in direct ELISAs and Western blots. Detects mouse and rat Complement Component C3d in immunohistochemistry.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Component C3d Antibody [Unconjugated]

• AHUS5;ARMD9;ASP;C3a;C3b;Complement C3;CPAMD1;HEL-S-62p
• Complement Component C3d

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Complement Component C3d Antibody (AF2655)(63)

Publications using AF2655

• Oizumi H, Miyamoto Y, Seiwa C et al. Lethal Adulthood Myelin Breakdown by Oligodendrocyte-specific Ddx54 Knockout iScience 7/1/2023 [PMID: 37720086]
• Jing‐Yi He, Dai‐Di Li, Qian Wen, Ting‐Yang Qin, Hong Long, Shi‐Bin Zhang et al. Synergistic effects of lipopolysaccharide and rotenone on dopamine neuronal damage in rats CNS Neuroscience & Therapeutics 8/1/2023 [PMID: 36942519]
• Zhu X, Schrader JM, Irizarry BA et al. Impact of A beta 40 and A beta 42 Fibrils on the Transcriptome of Primary Astrocytes and Microglia Biomedicines 11/19/2022 [PMID: 36428550]
  
  Details:
  Fifteen-month-old rTgF344-AD rat brain sections, dilution used 1:200
• Kofoed RH, Noseworthy K, Wu K et al. The engineered AAV2-HBKO promotes non-invasive gene delivery to large brain regions beyond ultrasound targeted sites Molecular Therapy - Methods & Clinical Development 12/8/2022 [PMID: 36284767]
• Zhang W, Xiao D, Li X et al. SIRT1 inactivation switches reactive astrocytes to an antiinflammatory phenotype in CNS autoimmunity Journal of Clinical Investigation 11/15/2022 [PMID: 36136587]
• Qi Zhang, Chang Liu, Rubing Shi, Shiyi Zhou, Huimin Shan, Lidong Deng et al. Blocking C3d+/GFAP+ A1 Astrocyte Conversion with Semaglutide Attenuates Blood-Brain Barrier Disruption in Mice after Ischemic Stroke Aging and disease 6/1/2022 [PMID: 35656116]
• T Wan, W Zhu, Y Zhao, X Zhang, R Ye, M Zuo, P Xu, Z Huang, C Zhang, Y Xie, X Liu Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice Nature Communications, 2022-03-03;13(1):1134. 2022-03-03 [PMID: 35241660] (ICC, Mouse)
• S Reuter, D Kentrup, A Grabner, G Köhler, K Buscher, B Edemir C4d Deposition after Allogeneic Renal Transplantation in Rats Is Involved in Initial Apoptotic Cell Clearance Cells, 2021-12-10;10(12):. 2021-12-10 [PMID: 34944007] (IHC, Rat)
• Kofoed R, Heinen S, Silburt J Et al. Transgene distribution and immune response after ultrasound delivery of rAAV9 and PHP.B to the brain in a mouse model of amyloidosis Molecular Therapy - Methods & Clinical Development 12/1/2021 [PMID: 34761053]
• Platt, JL;Zhao, C;Chicca, J;Pianko, MJ;Han, J;The, S;Rao, A;Keller, ET;Garcia de Mattos Barbosa, M;Naing, L;Pasieka-Axenov, T;Axenov, L;Schaefer, S;Farkash, E;Cascalho, M; Complement C3d enables cell-mediated immunity capable of distinguishing spontaneously transformed from nontransformed cells Proceedings of the National Academy of Sciences of the United States of America 2024-12-24 [PMID: 39693340] (Immunocytochemistry, Mouse)
• Yuan, Z;Lu, B;Zhang, M;Lu, Y;Wang, Z;Zhang, W;Cheng, H;Wu, Z;Ji, Q; Effect of NLRP3 inflammasome induced astrocyte phenotype alteration in morphine tolerance Frontiers in pharmacology 2024-11-12 [PMID: 39600361] (Immunohistochemistry, Western Blot, Rat)
• Platt, JL;Zhao, C;Chicca, J;Pianko, MJ;Han, J;The, S;Rao, A;Keller, E;de Mattos Barbosa, MG;Naing, L;Pasieka-Axenov, T;Axenov, L;Schaefer, S;Farkash, E;Cascalho, M; Complement C3d enables protective immunity capable of distinguishing spontaneously transformed from non-transformed cells bioRxiv : the preprint server for biology 2024-07-31 [PMID: 39211250] (Immunocytochemistry, Mouse)
• Jiao, M;Wang, C;Tang, X;Dai, C;Zhang, N;Fan, A;Qian, Z;Liu, S;Zhang, F;Li, B;Xu, Y;Tan, Z;Gong, F;Lu, Y;Zheng, F; Active secretion of IL-33 from astrocytes is dependent on TMED10 and promotes central nervous system homeostasis Brain, behavior, and immunity 2024-04-24 [PMID: 38663774] (Western Blot, Mouse, Transgenic Mouse)
• Feng, S;Wu, C;Zou, P;Deng, Q;Chen, Z;Li, M;Zhu, L;Li, F;Liu, TC;Duan, R;Yang, L; High-intensity interval training ameliorates Alzheimer's disease-like pathology by regulating astrocyte phenotype-associated AQP4 polarization Theranostics 2023-06-04 [PMID: 37351177] (IHC, Rat, Transgenic Mouse)
• X Sun, Y Ni, Q Lu, Y Liang, M Gu, X Xue, C Dai Mechanistic target of rapamycin complex 1 orchestrates the interplay between hepatocytes and Kupffer cells to determine the outcome of immune-mediated hepatitis Cell Death & Disease, 2022-12-09;13(12):1031. 2022-12-09 [PMID: 36494334] (IHC, Mouse)
• Kofoed RH, Noseworthy K, Wu K et al. The engineered AAV2-HBKO promotes non-invasive gene delivery to large brain regions beyond ultrasound targeted sites Molecular Therapy - Methods & Clinical Development 2022-12-08 [PMID: 36284767]
• Zhang W, Xiao D, Li X et al. SIRT1 inactivation switches reactive astrocytes to an antiinflammatory phenotype in CNS autoimmunity Journal of Clinical Investigation 2022-11-15 [PMID: 36136587]
• M Van Zeller, AM Sebastião, CA Valente Microglia Depletion from Primary Glial Cultures Enables to Accurately Address the Immune Response of Astrocytes Biomolecules, 2022-05-04;12(5):. 2022-05-04 [PMID: 35625594] (Western Blot, Mouse)
• AK Bongoni, IB Vikstrom, JL McRae, EJ Salvaris, N Fisicaro, MJ Pearse, S Wymann, T Rowe, AB Morelli, MP Hardy, PJ Cowan A potent truncated form of human soluble CR1 is protective in a mouse model of renal ischemia-reperfusion injury Scientific Reports, 2021-11-08;11(1):21873. 2021-11-08 [PMID: 34750424] (IHC, Mouse)
• F Shen, M Liu, F Pei, L Yu, X Yang Role of uromodulin and complement activation in the progression of kidney disease Oncology Letters, 2021-10-13;22(6):829. 2021-10-13 [PMID: 34691256] (IF, Mouse)
• Q Lu, Q Hou, K Cao, X Sun, Y Liang, M Gu, X Xue, AZ Zhao, C Dai Complement factor B in high glucose-induced podocyte injury and diabetic kidney disease JCI Insight, 2021-10-08;6(19):. 2021-10-08 [PMID: 34622800] (IHC, Mouse)
• HY Li, ZM Tang, Z Wang, JM Lv, XL Liu, YL Liang, B Cheng, N Gao, SR Ji, Y Wu C-reactive protein protects against acetaminophen-induced liver injury by preventing complement overactivation Cellular and Molecular Gastroenterology and Hepatology, 2021-09-16;0(0):. 2021-09-16 [PMID: 34536564] (ELISA Capture, Mouse)
• Anne Enzbrenner, Rahel Zulliger, Josef Biber, Ana Maria Quintela Pousa, Nicole Schäfer, Corinne Stucki et al. Sodium Iodate-Induced Degeneration Results in Local Complement Changes and Inflammatory Processes in Murine Retina International Journal of Molecular Sciences 8/26/2021 [PMID: 34502128]
• Garcia De Mattos Barbosa M, Lefferts Ar, Huynh D Et Al. TNFRSF13B genotypes control immune-mediated pathology by regulating the functions of innate B cells JCI insight 7/20/2021 [PMID: 34283811]
• Wen Q. Qiu, Shaopeiwen Luo, Stefanie A. Ma, Priyanka Saminathan, Herman Li, Jenny M. Gunnersen et al. The Sez6 Family Inhibits Complement by Facilitating Factor I Cleavage of C3b and Accelerating the Decay of C3 Convertases Frontiers in Immunology 4/15/2021 [PMID: 33936031]
• Ha Neui Kim, Erin M. Triplet, Maja Radulovic, Samantha Bouchal, Laurel S. Kleppe, Whitney L. Simon et al. The thrombin receptor modulates astroglia‐neuron trophic coupling and neural repair after spinal cord injury Glia 9/1/2021 [PMID: 33887067]
• N Hijaze, M Ledersnaid, E Simanovich, S Kassem, MA Rahat Inducing regulated necrosis and shifting macrophage polarization with anti-EMMPRIN antibody (161-pAb) and complement factors J Leukoc Biol, 2020-11-17;0(0):. 2020-11-17 [PMID: 33205451] (IHC, Mouse)
• Y Jabri, J Biber, N Diaz-Lezam, A Grosche, D Pauly Cell-Type-Specific Complement Profiling in the ABCA4-/- Mouse Model of Stargardt Disease Int J Mol Sci, 2020-11-11;21(22):. 2020-11-11 [PMID: 33187113] (IHC, Western Blot, Mouse)
• Y Lu, GR Sareddy, J Wang, Q Zhang, FL Tang, UP Pratap, RR Tekmal, RK Vadlamudi, DW Brann Neuron-Derived Estrogen is Critical for Astrocyte Activation and Neuroprotection of the Ischemic Brain J. Neurosci., 2020-08-19;0(0):. 2020-08-19 [PMID: 32817249] (IHC, Western Blot, Mouse)
• B Hou, Y Zhang, P Liang, Y He, B Peng, W Liu, S Han, J Yin, X He Inhibition of the NLRP3-inflammasome prevents cognitive deficits in experimental autoimmune encephalomyelitis mice via the alteration of astrocyte phenotype Cell Death Dis, 2020-05-15;11(5):377. 2020-05-15 [PMID: 32415059] (IHC, Western Blot, Mouse)
• Ali M. Alawieh, E. Farris Langley, Wuwei Feng, Alejandro M. Spiotta, Stephen Tomlinson Complement-Dependent Synaptic Uptake and Cognitive Decline after Stroke and Reperfusion Therapy The Journal of Neuroscience 5/13/2020 [PMID: 32291326]
• Jennetta W. Hammond, Matthew J. Bellizzi, Caroline Ware, Wen Q. Qiu, Priyanka Saminathan, Herman Li et al. Complement-dependent synapse loss and microgliosis in a mouse model of multiple sclerosis Brain, Behavior, and Immunity 7/1/2020 [PMID: 32151684]
• Heather L. Smith, Oliver J. Freeman, Adrian J. Butcher, Staffan Holmqvist, Ibrahim Humoud, Tobias Schätzl et al. Astrocyte Unfolded Protein Response Induces a Specific Reactivity State that Causes Non-Cell-Autonomous Neuronal Degeneration Neuron 3/4/2020 [PMID: 31924446]
• H Yoon, CI Choi, EM Triplet, MR Langley, LS Kleppe, HN Kim, WL Simon, IA Scarisbric Blocking the Thrombin Receptor Promotes Repair of Demyelinated Lesions in the Adult Brain J. Neurosci., 2020-01-07;0(0):. 1/7/2020 [PMID: 31911460]
• Yan Guo, Zara Mehrabian, Mary A. Johnson, David S. Albers, Cadmus C. Rich, Rudolf A. Baumgartner et al. Topical Trabodenoson Is Neuroprotective in a Rodent Model of Anterior Ischemic Optic Neuropathy (rNAION) Translational Vision Science & Technology 12/20/2019 [PMID: 31879569]
• Campbell SC, MuNoz-Ballester C, Chaunsali L, et al. Potassium and glutamate transport is impaired in scar-forming tumor-associated astrocytes Neurochem. Int. 12/9/2019 [PMID: 31825815]
• Chun-Cheih Chao, Cristina Gutiérrez-Vázquez, Veit Rothhammer, Lior Mayo, Michael A. Wheeler, Emily C. Tjon et al. Metabolic Control of Astrocyte Pathogenic Activity via cPLA2-MAVS Cell 12/1/2019 [PMID: 31813625]
• R Itto, Y Oe, K Imaruoka, E Sato, A Sekimoto, S Yamakage, S Kumakura, H Sato, S Ito, N Takahashi Glomerular Injury Is Exacerbated in Lupus-Prone MRL/lpr Mice Treated with a Protease-Activated Receptor 2 Antagonist Tohoku J. Exp. Med., 2019-10-01;249(2):127-133. 2019-10-01 [PMID: 31666446] (IHC, Mouse)
• Estefania Ugarte-Berzal, Lise Boon, Erik Martens, Vasily Rybakin, Daniel Blockmans, Jennifer Vandooren et al. MMP-9/Gelatinase B Degrades Immune Complexes in Systemic Lupus Erythematosus Frontiers in Immunology 3/22/2019 [PMID: 30967870]
• C Hu, L Li, P Ding, L Li, X Ge, L Zheng, X Wang, J Wang, W Zhang, N Wang, H Gu, F Zhong, M Xu, R Rong, T Zhu, W Hu Complement Inhibitor CRIg/FH Ameliorates Renal Ischemia Reperfusion Injury via Activation of PI3K/AKT Signaling J. Immunol., 2018-11-14;0(0):. 2018-11-14 [PMID: 30429287] (IHC-P, Mouse)
• Jennetta W. Hammond, Wen Q. Qiu, Daniel F. Marker, Jeffrey M. Chamberlain, Will Greaves-Tunnell, Matthew J. Bellizzi et al. HIV Tat causes synapse loss in a mouse model of HIV-associated neurocognitive disorder that is independent of the classical complement cascade component C1q. Glia 2018-12-01 [PMID: 30325063] (Western Blot, Mouse)
• Jennetta W. Hammond, Wen Q. Qiu, Daniel F. Marker, Jeffrey M. Chamberlain, Will Greaves-Tunnell, Matthew J. Bellizzi et al. HIV Tat causes synapse loss in a mouse model of HIV-associated neurocognitive disorder that is independent of the classical complement cascade component C1q. Glia 12/1/2018 [PMID: 30325063]
• Riccardo Natoli, Elizabeth Mason, Haihan Jiao, Aaron Chuah, Hardip Patel, Nilisha Fernando et al. Dynamic Interplay of Innate and Adaptive Immunity During Sterile Retinal Inflammation: Insights From the Transcriptome Frontiers in Immunology 7/18/2018 [PMID: 30073000]
• Riccardo Natoli, Elizabeth Mason, Haihan Jiao, Aaron Chuah, Hardip Patel, Nilisha Fernando et al. Dynamic Interplay of Innate and Adaptive Immunity During Sterile Retinal Inflammation: Insights From the Transcriptome Frontiers in Immunology 2018-07-18 [PMID: 30073000] (Western Blot, Mouse)
• A Alawieh, M Andersen, DL Adkins, S Tomlinson Acute Complement Inhibition Potentiates Neurorehabilitation and Enhances tPA-Mediated Neuroprotection J. Neurosci., 2018-06-19;38(29):6527-6545. 2018-06-19 [PMID: 29921716] (IHC, Mouse)
• A Alawieh, EF Langley, S Weber, D Adkins, S Tomlinson Identifying the role of complement in triggering neuroinflammation after traumatic brain injury J. Neurosci., 2018-02-06;0(0):. 2018-02-06 [PMID: 29437855] (IHC, Mouse)
• Tao Liao, Youqiu Xue, Daqiang Zhao, Siwen Li, Mingyu Liu, Jingrong Chen et al. In Vivo Attenuation of Antibody-Mediated Acute Renal Allograft Rejection by Ex Vivo TGF-beta -Induced CD4+Foxp3+ Regulatory T Cells Frontiers in Immunology 10/16/2017 [PMID: 29085374]
• Tao Liao, Youqiu Xue, Daqiang Zhao, Siwen Li, Mingyu Liu, Jingrong Chen et al. In Vivo Attenuation of Antibody-Mediated Acute Renal Allograft Rejection by Ex Vivo TGF-beta -Induced CD4+Foxp3+ Regulatory T Cells Frontiers in Immunology 2017-10-16 [PMID: 29085374] (Immunohistochemistry, Mouse)
• DJ Royer, MM Carr, HR Gurung, WP Halford, DJJ Carr The Neonatal Fc Receptor and Complement Fixation Facilitate Prophylactic Vaccine-Mediated Humoral Protection against Viral Infection in the Ocular Mucosa J. Immunol., 2017-07-31;0(0):. 7/31/2017 [PMID: 28760885]
• JL Platt, I Silva, SJ Balin, AR Lefferts, E Farkash, TM Ross, MC Carroll, M Cascalho C3d regulates immune checkpoint blockade and enhances antitumor immunity JCI Insight, 2017-05-04;2(9):. 2017-05-04 [PMID: 28469081] (IHC-Fr, Western Blot, Mouse)
• Ninette Genster, Elisabeth Præstekjær Cramer, Anne Rosbjerg, Katrine Pilely, Jack Bernard Cowland, Peter Garred Ficolins Promote Fungal Clearance in vivo and Modulate the Inflammatory Cytokine Response in Host Defense against <b><i>Aspergillus fumigatus</i></b> Journal of Innate Immunity 5/2/2016 [PMID: 27467404]
• Li Y, Song D, Song Y, Zhao L, Wolkow N, Tobias J, Song W, Dunaief J Iron-induced Local Complement Component 3 (C3) Up-regulation via Non-canonical Transforming Growth Factor (TGF)-beta Signaling in the Retinal Pigment Epithelium. J Biol Chem, 2015-03-23;290(19):11918-34. 2015-03-23 [PMID: 25802332] (IHC-P, Human)
• Abu-Humaidan A, Ananthoju N, Mohanty T, Sonesson A, Alberius P, Schmidtchen A, Garred P, Sorensen O The epidermal growth factor receptor is a regulator of epidermal complement component expression and complement activation. J Immunol, 2014-03-03;192(7):3355-64. 2014-03-03 [PMID: 24591374] (ICC, Mouse)
• Mikkel G. Terp, Kristina A. Olesen, Eva C. Arnspang, Rikke R. Lund, B. Christoffer Lagerholm, Henrik J. Ditzel et al. Anti-Human CD73 Monoclonal Antibody Inhibits Metastasis Formation in Human Breast Cancer by Inducing Clustering and Internalization of CD73 Expressed on the Surface of Cancer Cells The Journal of Immunology 10/15/2013 [PMID: 24043904]
• Zhang Y, Nester C, Holanda D, Marsh H, Hammond R, Thomas L, Meyer N, Hunsicker L, Sethi S, Smith R Soluble CR1 therapy improves complement regulation in C3 glomerulopathy. J Am Soc Nephrol, 2013-08-01;24(11):1820-9. 2013-08-01 [PMID: 23907509] (IHC, Mouse)
• Khan M, Maasch C, Vater A, Klussmann S, Morser J, Leung L, Atkinson C, Tomlinson S, Heeger P, Nicolls M Targeting complement component 5a promotes vascular integrity and limits airway remodeling. Proc Natl Acad Sci U S A, 2013-03-25;110(15):6061-6. 2013-03-25 [PMID: 23530212] (IHC, Mouse)
• Atkinson , Carl, Floerchinger , Bernhard, Qiao , Fei, Casey , Sarah, Williamson , Tucker, Moseley , Ellen, Stoica , Serban, Goddard , Martin, Ge , Xupeng, Tullius , Stefan G, Tomlinson , Stephen Donor brain death exacerbates complement-dependent ischemia/reperfusion injury in transplanted hearts. Circulation, 2013-02-26;127(12):1290-9. 2013-02-26 [PMID: 23443736] (IHC-P, Mouse)
• Elvington , Andrew, Atkinson , Carl, Zhu , Hong, Yu , Jin, Takahashi , Kazue, Stahl , Gregory, Kindy , Mark S, Tomlinson , Stephen The alternative complement pathway propagates inflammation and injury in murine ischemic stroke. J Immunol, 2012-10-01;189(9):4640-7. 2012-10-01 [PMID: 23028050] (IHC-P, Mouse)
• Elvington A, Atkinson C, Kulik L Pathogenic natural antibodies propagate cerebral injury following ischemic stroke in mice. J. Immunol., 2011-12-23;188(3):1460-8. 2011-12-23 [PMID: 22198950] (IHC-Fr, IHC-P, Mouse)
• Eileen M. Bauer, Han Zheng, Suzy Comhair, Serpil Erzurum, Timothy R. Billiar, Philip M. Bauer Complement C3 Deficiency Attenuates Chronic Hypoxia-Induced Pulmonary Hypertension in Mice PLoS ONE 12/14/2011 [PMID: 22194859]
• Zhao Z, Chen Y, Wang J Age-related retinopathy in NRF2-deficient mice. PLoS ONE, 2011-04-29;6(4):e19456. 2011-04-29 [PMID: 21559389] (IHC-Fr, Mouse)
• Leung VW, Yun S, Botto M, Mason JC, Malik TH, Song W, Paixao-Cavalcante D, Pickering MC, Boyle JJ, Haskard DO Decay-accelerating factor suppresses complement C3 activation and retards atherosclerosis in low-density lipoprotein receptor-deficient mice. Am. J. Pathol., 2009-09-03;175(4):1757-67. 2009-09-03 [PMID: 19729477] (ELISA Development, Mouse)

Bioinformatics

• Uniprot:
  • P01027()