Coilin Antibody (HL2384)

HeLa whole cell and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Coilin antibody [HL2384] (NBP3-48679) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Coilin antibody [HL2384] detects Coilin protein at Cajal bodies by immunofluorescent analysis.Sample: A431 cells were fixed in ice-cold MeOH for 5 min.Green: Coilin stained by Coilin antibody [HL2384] (NBP3-48679) diluted at 1:500.Blue: Fluoroshield with DAPI .

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: HL2384
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Coilin Antibody (HL2384) Summary

• Description: Novus Biologicals Rabbit Coilin Antibody (HL2384) (NBP3-48679) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human Coilin.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: COIL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for Coilin Antibody (HL2384)

• CLN80p80-coilin
• coilin p80
• coilin
• p80

Background

Coilin is encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: COIL