ARHGEF5 Antibody

Western Blot: ARHGEF5 Antibody [NB100-2559] - (0.1ug/ml) staining of HeLa Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: ARHGEF5 Antibody [NB100-2559] - (3.75ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ARHGEF5 Antibody Summary

• Immunogen: Peptide with sequence C-DKEIDQNSQQEE corresponding to internal region according to NP_005426.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARHGEF5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 170 kDa band observed in human HeLa lysates (calculated MW of 177 kDa band according to NP_005426.2).
  IHC: Paraffin embedded Human Placenta.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARHGEF5 Antibody

• ephexin-3
• GEF5
• Guanine nucleotide regulatory protein TIM
• Oncogene TIM
• p60 TIM
• P60
• Rho guanine nucleotide exchange factor (GEF) 5
• rho guanine nucleotide exchange factor 5
• TIM1
• TIMDKFZp686N1969
• Transforming immortalized mammary oncogene

Background

Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARHGEF5 Antibody (NB100-2559)(1)

Publication using NB100-2559

• Debily MA, Camarca A, Ciullo M et al. Expression and molecular characterization of alternative transcripts of the ARHGEF5/TIM oncogene specific for human breast cancer. Hum Mol Genet 2004-02-01 [PMID: 14662653]

Bioinformatics

• Gene Symbol: ARHGEF5
• Entrez:
  • 7984(Human)
• Uniprot:
  • NP_005426.2(Human)