Ku80/XRCC5 Antibody - BSA Free

Images

 
Western Blot: Ku80/XRCC5 Antibody [NB100-508] - MDA-MB-231, MCF-7, SUM149, SUM159, T47D, HCC1954, 4T1, B16-F10, Hep3b and 293T cells lysates (20 ug/lane). 10% SDS-PAGE. KU80 (NB100-508) primary antibody at 1:2000 ...read more
Western Blot: Ku80/XRCC5 Antibody [NB100-508] - Analysis of Ku80 using NB100-508 at 1:500 dilution on 50 ug of total lysates of A431 (lane 1) and AR42J (lane 2) cells.
Immunoprecipitation: Ku80/XRCC5 Antibody [NB100-508] - Detection of Ku80 in either CHO or A549 nuclear extracts (NE) or cytoplasmic lysates (cyto) using NB 100-508. A Ku80 deficient cell lysate, XRS, was used as a ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, HaSpecies Glossary
Applications WB, ChIP, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Ku80/XRCC5 Antibody - BSA Free Summary

Immunogen
A synthetic peptide corresponding to residues 419-440 LVYVQLPFMEDLRQYMFSSLKN] of the 80 kDa Ku80 protein.
Specificity
Reacts with residues 419-440 [LVYVQLPFMEDLRQYMFSSLKN] of the 80 kDa Ku80 protein. Sequence is 100% conserved between human, mouse, rat and hamster.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
XRCC5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation
  • Immunoprecipitation
  • Western Blot 1:500
Application Notes
Use in Chromatin Immunoprecipitation reported in scientific literature (PMID:35031618) This antibody has only been tested in Western blot.
Control Peptide
Ku80/XRCC5 Peptide (NB100-508PEP)
Reviewed Applications
Read 1 Review rated 5
using
NB100-508 in the following applications:

Publications
Read Publications using
NB100-508 in the following applications:

  • IP
    2 publications
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Ku80/XRCC5 Antibody - BSA Free

  • 80kDa
  • ATP-dependent DNA helicase 2 subunit 2
  • ATP-dependent DNA helicase II 80 kDa subunit
  • CTC85
  • CTCBF
  • EC 3.6.4.-
  • FLJ39089
  • G22P2
  • KARP1
  • KARP-1
  • Ku80
  • Ku86 autoantigen related protein 1,86 kDa subunit of Ku antigen
  • Ku86
  • Ku86DNA repair protein XRCC5
  • KUB2
  • NFIV
  • Thyroid-lupus autoantigen
  • TLAA
  • X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining)CTC box-binding factor 85 kDa subunit
  • X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining; Ku autoantigen, 80kD)
  • X-ray repair cross-complementing protein 5
  • X-ray repair, complementing defective, repair in Chinese hamster
  • XRCC5

Background

Telomere length maintenance, an activity essential for chromosome stability and genome integrity, is regulated by telomerase- and telomere-associated factors. The DNA repair protein Ku (a heterodimer of Ku70 and Ku80 subunits) associates with mammalian telomeres and contributes to telomere maintenance. The Ku heterodimer functions at two kinds of DNA ends: telomeres and double-strand breaks. The role that Ku plays at these two classes of termini must be distinct, because Ku is required for accurate and efficient joining of double-strand breaks while similar DNA repair events are normally prohibited at chromosome ends.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ku80/XRCC5 Antibody (NB100-508)(3)

Review for Ku80/XRCC5 Antibody (NB100-508) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human and Mouse.

Reviews using NB100-508:
Filter by Applications
WB
(1)
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Filter by Species
Human and Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot Ku80/XRCC5 NB100-508
Enlarge
5
reviewed by:
Yongkang Yang
WB Human and Mouse 10/10/2020
View

Summary

ApplicationWestern Blot
Sample Tested20 ug whole cell lysate,cell line, whole cell lysate
SpeciesHuman and Mouse
LotR6141

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Secondary Antibodies

 

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Bioinformatics Tool for Ku80/XRCC5 Antibody (NB100-508)

Discover related pathways, diseases and genes to Ku80/XRCC5 Antibody (NB100-508). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Yongkang Yang
10/10/2020
Application: WB
Species: Human and Mouse

Bioinformatics

Gene Symbol XRCC5
Entrez