KRAS Antibody (3B10-2F2)

Images

 
Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - KRAS monoclonal antibody (M01), clone 3B10-2F2 Analysis of KRAS expression in HeLa.
Immunocytochemistry/ Immunofluorescence: KRAS Antibody (3B10-2F2) [H00003845-M01] - Analysis of monoclonal antibody to KRAS on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western blot analysis of KRAS expression in lysates from human lung cancer cell lines: H23, H358, A549, and H441. Image from verified customer review.
Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Analysis of KRAS expression in transfected 293T cell line by KRAS monoclonal antibody (M01), clone 3B10-2F2.Lane 1: KRAS transfected lysate(21 KDa).Lane 2: ...read more
ELISA: KRAS Antibody (3B10-2F2) [H00003845-M01] - Detection limit for recombinant GST tagged KRAS is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
3B10-2F2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

KRAS Antibody (3B10-2F2) Summary

Immunogen
KRAS (AAH13572, 1 a.a. - 188 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGHEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM
Specificity
KRAS - v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRAS
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunoprecipitation
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for IF and ELISA. IP usage was reported in scientific literature. Customer feedback on frozen sections has been negative.
Positive Control
KRAS Lysate (NBP2-64728)
Reviewed Applications
Read 1 Review rated 4
using
H00003845-M01 in the following applications:

Publications
Read Publications using
H00003845-M01 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for KRAS Antibody (3B10-2F2)

  • cellular c-Ki-ras2 proto-oncogene
  • c-Ki-ras
  • C-K-RAS
  • GTPase KRas
  • KI-RAS
  • Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog
  • K-Ras 2
  • K-ras p21 protein
  • KRAS1
  • K-RAS2A
  • K-RAS2B
  • KRAS2NS3
  • K-RAS4A
  • K-RAS4B
  • NS
  • oncogene KRAS2
  • PR310 c-K-ras oncogene
  • RASK2c-Kirsten-ras protein
  • transforming protein p21
  • v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog
  • v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Background

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00003845-M01
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Publications for KRAS Antibody (H00003845-M01)(10)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IP, WB.


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(1)
IP
(1)
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Showing Publications 1 - 10 of 10.
Publications using H00003845-M01 Applications Species
Garcia-Berrocoso T, Llombart V, Colas-Campas L et al. Single Cell Immuno-laser Microdissection Coupled to Label-free Proteomics to Reveal the Proteotypes of Human Brain Cells After Ischemia Mol. Cell Proteomics. 2017 Nov 13 [PMID: 29133510] (ICC/IF, Human) ICC/IF Human
Kuracha MR, Thomas P, Loggie BW, Govindarajan V et al. Bilateral blockade of MEK- and PI3K-mediated pathways downstream of mutant KRAS as a treatment approach for peritoneal mucinous malignancies. PLoS One 2017 Jun 22 [PMID: 28640835]
Forzati F, De Martino M, Esposito F et al. miR-155 is positively regulated by CBX7 in mouse embryonic fibroblasts and colon carcinomas, and targets the KRAS oncogene. BMC Cancer 2017 Mar 4 [PMID: 28259135]
Madureira PA, Bharadwaj AG, Bydoun M et al. Cell surface protease activation during RAS transformation: Critical role of the plasminogen receptor, S100A10. Oncotarget 2016 Jun 24 [PMID: 27351226]
Ambrosini G, Khanin R, Carvajal RD, Schwartz GK. Overexpression of DDX43 mediates MEK-Inhibitor Resistance through RAS up-regulation in Uveal Melanoma Cells. Mol Cancer Ther. 2014 Jun 04 [PMID: 24899684]
King S, Bray S, Galbraith S et al. Evidence for aldosterone-dependent growth of renal cell carcinoma. Int J Exp Pathol. 2014 May 07 [PMID: 24802662]
Subramani A, Alsidawi S, Jagannathan S et al. The brain microenvironment negatively regulates miRNA-768-3p to promote K-ras expression and lung cancer metastasis. Sci Rep. 2013 Aug 9 [PMID: 23928793] (WB, Human) WB Human
Valtorta E, Misale S, Sartore-Bianchi A et al. KRAS gene amplification in colorectal cancer and impact on response to EGFR-targeted therapy. Int J Cancer. 2013 Jan 21 [PMID: 23404247]
Hsu SP, Lee WS. Progesterone receptor activation of extranuclear signaling pathways in regulating p53 expression in vascular endothelial cells Mol Endocrinol 2011 Mar [PMID: 21239614] (IP, WB, Human) IP, WB Human
Fuentes-Calvo I, Blazquez-Medela AM, Santos E et al. Analysis of k-ras nuclear expression in fibroblasts and mesangial cells. PLoS One 5(1):e8703. 2010 Jan 14. [PMID: 20090846]

Review for KRAS Antibody (H00003845-M01) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00003845-M01:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot KRAS H00003845-M01
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reviewed by:
Anon ymous
WB Human 03/14/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from the following human lung cancer lines: H23, H358, A549, H441.
SpeciesHuman
CommentsBlocking with 5% nonfat milk reconstituted in TBST works well as an alternative, although it resulted in slightly elevated nonspecific background levels in our hands.

Blocking

Blocking Details100mL Tris buffered saline, 5g BSA, 0.1mL Tween 20. Incubate 2h at room temperature.

Primary Anitbody

Dilution RatioDilute 1:500 in TBST; Incubate overnight at 4 C with gentle agitation.

Secondary Antibody

Secondary DescriptionChicken Anti-mouse HRP
Secondary Manufacturer Cat#NBP1-75102
Secondary Concentration1:1000 in TBST

Details

Detection NotesAmersham ECL, Hyperfilm ECL, 1 minute exposure. Positive control (GAPDH) exposed for 15 seconds.

Comments

CommentsBlocking with 5% nonfat milk reconstituted in TBST works well as an alternative, although it resulted in slightly elevated nonspecific background levels in our hands.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for KRAS Antibody (H00003845-M01) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for KRAS Antibody (H00003845-M01)

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Autophagy: Pro or Anti-tumorigenic? And the role of epigenetics in this debate
By Christina Towers, PhDAutophagy is an evolutionarily conserved process that cells use to break down damaged cytoplasmic constituents in order to fuel cellular metabolism, particularly in instances of stress. This process has been heavily...  Read full blog post.

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Anon ymous
03/14/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol KRAS
Entrez
OMIM
Uniprot