KRAS Antibody (3B10-2F2)

Images

 
Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - KRAS monoclonal antibody (M01), clone 3B10-2F2 Analysis of KRAS expression in HeLa.
Immunocytochemistry/ Immunofluorescence: KRAS Antibody (3B10-2F2) [H00003845-M01] - Analysis of monoclonal antibody to KRAS on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Western blot analysis of KRAS expression in lysates from human lung cancer cell lines: H23, H358, A549, and H441. Image from verified customer review.
Western Blot: KRAS Antibody (3B10-2F2) [H00003845-M01] - Analysis of KRAS expression in transfected 293T cell line by KRAS monoclonal antibody (M01), clone 3B10-2F2.Lane 1: KRAS transfected lysate(21 KDa).Lane 2: ...read more
Sandwich ELISA: KRAS Antibody (3B10-2F2) [H00003845-M01] - Detection limit for recombinant GST tagged KRAS is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IP
Clone
3B10-2F2
Clonality
Monoclonal
Host
Mouse

Order Details

KRAS Antibody (3B10-2F2) Summary

Immunogen
KRAS (AAH13572, 1 a.a. ~ 188a.a) full length recombinant protein with GST tag.MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGHEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKCVIM
Specificity
KRAS - v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRAS
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunoprecipitation 1:10-1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA. Immunoprecipitation was reported in scientific literature. Customer feedback on frozen sections has been negative.
Reviewed Applications
Read 1 Review rated 4
using
H00003845-M01 in the following applications:

Publications
Read Publications using
H00003845-M01 in the following applications:

  • IP
    1 publication
  • WB
    2 publications

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for KRAS Antibody (3B10-2F2)

  • cellular c-Ki-ras2 proto-oncogene
  • c-Ki-ras
  • C-K-RAS
  • GTPase KRas
  • KI-RAS
  • Kirsten rat sarcoma-2 viral (v-Ki-ras2) oncogene homolog
  • K-Ras 2
  • K-ras p21 protein
  • KRAS1
  • K-RAS2A
  • K-RAS2B
  • KRAS2NS3
  • K-RAS4A
  • K-RAS4B
  • NS
  • oncogene KRAS2
  • PR310 c-K-ras oncogene
  • RASK2c-Kirsten-ras protein
  • transforming protein p21
  • v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog
  • v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog

Background

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for KRAS Antibody (H00003845-M01)(6)

Reviews for KRAS Antibody (H00003845-M01) (1) 41

Average Rating: 4
(Based on 1 reviews)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using H00003845-M01:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot KRAS H00003845-M01
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reviewed by:
Anon ymous
WB Human 03/14/2014
View

Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from the following human lung cancer lines: H23, H358, A549, H441.
SpeciesHuman
CommentsBlocking with 5% nonfat milk reconstituted in TBST works well as an alternative, although it resulted in slightly elevated nonspecific background levels in our hands.

Blocking

Blocking Details100mL Tris buffered saline, 5g BSA, 0.1mL Tween 20. Incubate 2h at room temperature.

Primary Anitbody

Dilution RatioDilute 1:500 in TBST; Incubate overnight at 4 C with gentle agitation.

Secondary Antibody

Secondary DescriptionChicken Anti-mouse HRP
Secondary Manufacturer Cat#NBP1-75102
Secondary Concentration1:1000 in TBST

Details

Detection NotesAmersham ECL, Hyperfilm ECL, 1 minute exposure. Positive control (GAPDH) exposed for 15 seconds.

Comments

CommentsBlocking with 5% nonfat milk reconstituted in TBST works well as an alternative, although it resulted in slightly elevated nonspecific background levels in our hands.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for KRAS Antibody (H00003845-M01) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Blogs on KRAS

There are no specific blogs for KRAS, but you can read our latest blog posts.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol KRAS
Entrez
OMIM
Uniprot

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