IFIT3 Antibody (OTI1G1)

Western Blot: IFIT3 Antibody (OTI1G1) [NBP2-02148] - Analysis of extracts (35ug) from 9 different cell lines by using anti-IFIT3 monoclonal antibody at 1:200 dilution. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human)

Immunocytochemistry/Immunofluorescence: IFIT3 Antibody (OTI1G1) [NBP2-02148] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IFIT3.

Flow Cytometry: IFIT3 Antibody (1G1) [NBP2-02148] - Analysis of Jurkat cells, using anti-IFIT3 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Western Blot: IFIT3 Antibody (1G1) [NBP2-02148] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFIT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFIT3.

Immunocytochemistry/Immunofluorescence: IFIT3 Antibody (1G1) [NBP2-02148] - Immunofluorescent staining of HT29 cells using anti-IFIT3 mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF
• Clone: OTI1G1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

IFIT3 Antibody (OTI1G1) Summary

• Description: Novus Biologicals Mouse IFIT3 Antibody (OTI1G1) (NBP2-02148) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. Anti-IFIT3 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human IFIT3 (NP_001540) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IFIT3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:2000
• Theoretical MW: 56 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IFIT3 Antibody (OTI1G1)

• CIG-49
• GARG-49
• IFI60Retinoic acid-induced gene G protein
• IFIT-3
• IFIT-4
• IFIT4IFI-60K
• interferon-induced protein with tetratricopeptide repeats 3
• Interferon-induced protein with tetratricopeptide repeats 4Interferon-induced 60 kDa protein
• IRG2
• ISG60
• ISG-60
• RIG-GCIG49

Background

IFIT3, also known as IFIT4, IFI60, and RIGG, has been identified as a key element in the IFN-alpha anti-viral pathway. Additionally, IFIT3 binding negatively regulates the apoptotic effects of ISG54. IFIT3 is also a key target of STAT1, which is important in regulating IFN responses. One study has shown that expression of RIGG in a human monocytoma line led to growth arrest at the G1/S transition.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IFIT3 Antibody (NBP2-02148)(2)

Publications using NBP2-02148

• Boukhaled GM, Gadalla R, Elsaesser HJ et al. Pre-encoded responsiveness to type I interferon in the peripheral immune system defines outcome of PD1 blockade therapy Nature immunology 2022-07-14 [PMID: 35835962] (FLOW, Human)
  
  Details:
  Flow Dilution 1:100
• Hagner PR Man HW Fontanillo C et al. et al. CC-122, a pleiotropic pathway modifier, mimics an interferon response and has antitumor activity in DLBCL. Blood. 2015-08-06 [PMID: 26002965] (WB, Human)

Bioinformatics

• Gene Symbol: IFIT3
• Entrez:
  • 3437(Human)