Coactosin-like Protein 1/CotL1 Antibody

Images

 
Western Blot: Coactosin-like Protein 1/CotL1 Antibody [NBP1-54851] - Analysis of HeLa cell lysate (30ug) using anti-COTL1 antibody. Image from verified customer review.
Western Blot: Coactosin-like Protein 1/CotL1 Antibody [NBP1-54851] - Human Thymus lysate, concentration 0.2-1 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Eq, GP, Rb, ZeSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Coactosin-like Protein 1/CotL1 Antibody Summary

Immunogen
Synthetic peptides corresponding to COTL1(coactosin-like 1 (Dictyostelium)) The peptide sequence was selected from the N terminal of COTL1. Peptide sequence MATKIDKEACRAAYNLVRDDGSAVIWVTFKYDGSTIVPGEQGAEYQHFIQ.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
COTL1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
Application Notes
This is a rabbit polyclonal antibody against COTL1 and was validated on Western blot.
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-54851 in the following application:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 2% Sucrose
Preservative
0.09% Sodium Azide
Purity
Immunogen affinity purified

Notes

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Alternate Names for Coactosin-like Protein 1/CotL1 Antibody

  • CLP
  • CLPFLJ43657
  • Coactosin like Protein 1
  • coactosin-like 1 (Dictyostelium)
  • Coactosin-like Protein 1
  • coactosin-like protein
  • COTL1
  • MGC19733

Background

This gene encodes one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis. Although this gene has

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Coactosin-like Protein 1/CotL1 Antibody (NBP1-54851) (0)

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Review for Coactosin-like Protein 1/CotL1 Antibody (NBP1-54851) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-54851:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot Coactosin-like Protein 1/CotL1 NBP1-54851
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reviewed by:
Anonymous
WB Human 10/07/2014
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Summary

ApplicationWestern Blot
Sample TestedHela Cell Lysate
SpeciesHuman

Blocking

Blocking DetailsBSA (5%) in PBS buffer

Primary Anitbody

Dilution Ratio1:800 diluted in PBS buffer

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP conjugated secondary antibody (Biopioneer)
Secondary Manufacturer Cat#GAR21002-1
Secondary Concentration1:15000

Details

Detection NotesDeveloped using FemtoLucent Plus HRP Chemiluminescent Kit with a 1 min exposure

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Video Protocols

WB Video Protocol

FAQs for Coactosin-like Protein 1/CotL1 Antibody (NBP1-54851) (0)

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Secondary Antibodies

 

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
10/07/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol COTL1
Uniprot