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Complement Component C5aR1 Antibody (347214) [Unconjugated]

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Recombinant Human Complement Component C5a (Catalog # 2037-C5) induces N-acetyl-beta -D-glucosa­minidase release in the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a dose-dependent manner (orange line). N-Acetyl-beta -D-Glucosaminidase Release elicited by Recombinant Human Complement Component C5a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti‑Human Complement Component C5a R1 Monoclonal Antibody (Catalog # MAB3648). The ND50 is typically 2‑10 µg/mL in the presence of cytochalasin‑B.
Recombinant Human Complement Component C5a (Catalog # 2037-C5) induces N-acetyl-beta -D-glucosa­minidase release in the dibutyryl cyclic-AMP differentiated U937 human histiocytic lymphoma cell line in a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, B/N, CyTOF-ready
Clone
347214
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB3648U-100UG
Alexa Fluor 405
FAB3648V-100UG
Alexa Fluor 488
FAB3648G-100UG
Alexa Fluor 594
FAB3648T-100UG
Alexa Fluor 647
FAB3648R-100UG
Alexa Fluor 700
FAB3648N-100UG
Alexa Fluor 750
FAB3648S-100UG
Allophycocyanin
FAB3648A
Fluorescein
FAB3648F
Phycoerythrin
FAB3648P
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
FAB3648F
Carrier Free
FAB3648P
Carrier Free
MAB3648-SP
Carrier Free
FAB3648A
Carrier Free
FAB3648V-100UG
Carrier Free
FAB3648U-100UG
Carrier Free
FAB3648T-100UG
Carrier Free
FAB3648S-100UG
Carrier Free
FAB3648R-100UG
Carrier Free
FAB3648N-100UG
Carrier Free
FAB3648G-100UG
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Complement Component C5aR1 Antibody (347214) [Unconjugated] Summary

Immunogen
NS0 mouse myeloma cell line transfected with human Complement Component C5a R1
Met1-Val350
Accession # P21730
Specificity
Detects human Complement Component C5a R1. Stains human Complement Component C5a R1 transfected cells but not irrelevant transfectants.
Details of Functionality
ActivityAssaywCitation not found None None
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
C5AR1
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready
  • Neutralization 2-10 ug/mL
Publications
Read Publication using
MAB3648 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Complement Component C5aR1 Antibody (347214) [Unconjugated]

  • C5a anaphylatoxin chemotactic receptor
  • C5A
  • C5aR
  • C5a-R
  • C5AR1
  • C5ARC5a anaphylatoxin receptor
  • C5R1C5a ligand
  • CD88 antigen
  • CD88
  • complement component 5 receptor 1 (C5a ligand)
  • complement component 5 receptor 1
  • complement component 5a receptor 1
  • Complement Component C5a R1
  • Complement Component C5aR1

Background

Complement Component C5a R1 (C5aR1), also known as C5a ligand and CD88, is a 7TM protein expressed on myeloid, endothelial, epithelial, and smooth muscle cells. C5aR binds the activated complement anaphylatoxin C5a. In established allergic environments, this triggers neutrophil and eosinophil chemotaxis and the release of proinflammatory mediators. In contrast, C5aR1/C5a interactions are protective during allergen sensitization. Human C5aR1 shares 66% amino acid sequence identity with mouse and rat C5aR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow (Intracellular): Complement C3 Antibody (11H9) [NB200-540] - An intracellular stain was performed on RAW 246.7 cells with Complement C3 (11H9-3-2) antibody NB200-540 (blue) and a matched isotype control NBP2-31382 (orange). Cells were either treated with 3uM Monensin for 3 hours to block the secretion of Complement C3 (B) or grown in normal media (A). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) PE-conjugated secondary antibody (F0102B, R&amp;D Systems).
Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (11H9) [NB200-540] - C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Staining with antibody 11H9. Glomerular staining pattern. Fixation in 4% paraformaldehyde in PBS pH 7.4. Vibratome sections of 4 um. Pretreated with 3% hydrogen peroxide for 20 min to quench endogenous peroxidases. Microwaved in antigen unmasking solution for 2-5 minutes as antigen retrieval.
Immunohistochemistry-Paraffin: Complement C3 Antibody (11H9) [NB200-540] - Complement C3 protein in a FFPE tissue section of mouse liver using 1:100 dilution of Complement C3 antibody (clone 11H9) NB200-540. Weak but distinct membrane-cytoplasmic immunopositivity was observed in hepatocytes and few cells developed punctate membrane staining.
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TNF-alpha
[Unconjugated]
210-TA
1 µg/lane of Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a band at 17 kDa.
Recombinant Human TNF-alpha (Catalog # 210‑TA) induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 25‑100&amp;nbsp;pg/mL.
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Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
Flow (Intracellular): NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on THP-1 cells with NOD2 (2D9) antibody NB100-524AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes.
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7268-CT
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NBP2-45041
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CXCL8/IL-8
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Recombinant Human IL-8/CXCL8 (Catalog&amp;nbsp;# 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog&amp;nbsp;# AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20&amp;nbsp;ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog&amp;nbsp;# MAB208). The ND50 is typically0.08-0.4&amp;nbsp;µg/mL.
IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) at 10&amp;nbsp;µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights&amp;#8482; 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog&amp;nbsp;# NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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IL-6 [Unconjugated]
7270-IL/CF
IL-6 [Unconjugated]
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FPR1 Antibody
NB100-56473
Western Blot: FPR1 Antibody [NB100-56473] - Analysis of FPR1 in the 1) absence and 2) presence of immunizing peptide in human liver lysate using this antibody at 1 ug/ml.
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Flow Cytometry: CD19 Antibody (CB19) [NBP2-25196] - A surface stain was performed on Ramos cells with CD19 Antibody (CB19) NBP2-26646 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.
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Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2&amp;nbsp;mg/mL. A specific band was detected for Myeloperoxidase/MPO at approximately 67&amp;nbsp;kDa (as indicated) using 5&amp;nbsp;µg/mL of Goat Anti-Human/Mouse Myeloperoxidase/MPO Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3667) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog&amp;nbsp;# HAF109). This experiment was conducted under reducing&amp;nbsp;conditions and using the12-230 kDa separation system.
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Publications for Complement Component C5aR1 Antibody (MAB3648)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB3648U
Alexa Fluor 405 FAB3648V
Alexa Fluor 488 FAB3648G
Alexa Fluor 594 FAB3648T
Alexa Fluor 647 FAB3648R
Alexa Fluor 700 FAB3648N
Alexa Fluor 750 FAB3648S
Allophycocyanin FAB3648A
Fluorescein FAB3648F
Phycoerythrin FAB3648P
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol C5AR1
Uniprot