Claspin Antibody - BSA Free

Western Blot: Claspin Antibody [NB100-248] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Affinity purified rabbit anti-Claspin antibody NB100-248 (lot number 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. Claspin was also immunoprecipitated by a previous lot (lot number 1) of this antibody. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Claspin Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Claspin Antibody - BSA Free (NB100-248) is a polyclonal antibody validated for use in WB, ICC/IF and IP. Anti-Claspin Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residues 900 and 950 of human Claspin using the numbering given in entry AAG24515.1 (GeneID 63967).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CLSPN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation 2-10 ug/mg of lysate
  • Western Blot 1:1000-1:10000
• Application Notes: Use in ICC/IF reported in scientific literature (PMID:31825846).
• Theoretical MW: 180 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:31825846).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Claspin Antibody - BSA Free

• claspin homolog (Xenopus laevis)
• claspin homolog
• Claspin
• CLSPN
• hClaspin
• MGC131612
• MGC131613
• MGC131615

Background

Claspin is a cell cycle regulated protein. It plays a critical role in replication checkpoint control and interacts with the checkpoint proteins ATR and Rad9. It also associates with Chk1, but only following replication stress or other types of DNA damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Claspin Antibody (NB100-248)(5)

Publications using NB100-248

• Giuliani V, Miller MA, Liu CY Et al. PRMT1-dependent regulation of RNA metabolism and DNA damage response sustains pancreatic ductal adenocarcinoma Nature communications 2021-07-30 [PMID: 34330913] (WB, Human)
• Rai R, Gu P, Broton C The Replisome Mediates A-NHEJ Repair of Telomeres Lacking POT1-TPP1 Independently of MRN Function Cell Rep 2019-12-10 [PMID: 31825846] (ICC/IF, WB, Mouse, Human)
• Benevolo M, Musio A, Vocaturo A et al. Claspin as a biomarker of human papillomavirus-related high grade lesions of uterine cervix. J Transl Med 2012-06-01 [PMID: 22731782]
• Gao D, Inuzuka H, Korenjak M et al. Cdh1 regulates cell cycle through modulating the claspin/Chk1 and the Rb/E2F1 pathways. Mol Biol Cell 2009-07-01 [PMID: 19477924]
• Jiang et al. J Biol Chem. 2003-01-01

Bioinformatics

• Gene Symbol: CLSPN
• Entrez:
  • 63967(Human)
• Uniprot:
  • AAG24515.1(Human)