MCM7 Antibody (6C2)

Images

 
Western Blot: MCM7 Antibody (6C2) [H00004176-M01] - MCM7 monoclonal antibody (M01), clone 6C2 Analysis of MCM7 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: MCM7 Antibody (6C2) [H00004176-M01] - Analysis of monoclonal antibody to MCM7 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: MCM7 Antibody (6C2) [H00004176-M01] - Analysis of monoclonal antibody to MCM7 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. Antibody concentration 3 ug/ml.
Proximity Ligation Assay: MCM7 Antibody (6C2) [H00004176-M01] - Analysis of protein-protein interactions between CDK7 and MCM7. HeLa cells were stained with anti-CDK7 rabbit purified polyclonal 1:1200 and anti-MCM7 ...read more
Proximity Ligation Assay: MCM7 Antibody (6C2) [H00004176-M01] - Analysis of protein-protein interactions between CDC6 and MCM7. Huh7 cells were stained with anti-CDC6 rabbit purified polyclonal 1:600 and anti-MCM7 mouse ...read more
Sandwich ELISA: MCM7 Antibody (6C2) [H00004176-M01] - Detection limit for recombinant GST tagged MCM7 is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC-P, PLA
Clone
6C2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

MCM7 Antibody (6C2) Summary

Immunogen
MCM7 (AAH09398, 1 a.a. - 389 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MALKDYALEKEKVKKFLQEFYQDDELGKKQFKYGNQLVRLAHREQVALYVDLDDVAEDDPELVDSICENARRYAKLFADAVQELLPQYKEREVVNKDVLDVYIEHRLMMEQRSRDPGMVRSPQNQYPAELMRRFELYFQGPSSSKPRVIREVRADSVGKLVTVRGIVTRVSEVKPKMVVATYTCDQCGAETYQPIQSPTFMPLIMCPSQECQTNRSGGRLYLQTRGSRFIKFQEMKMQEHSDQVPVGNIPRSITVLVEGENTRIAQPGDHVSVTGIFLPILRTGFRQVVQGLLSETYLEAHRIVKMNKSEDDESGAGELTREELRQIADVIFATVRELVSGGRSVRFSEAEQRCVSRGFTPAQFQAALDEYEELNVWQVNASRTRITFV
Specificity
MCM7 - MCM7 minichromosome maintenance deficient 7 (S. cerevisiae)
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MCM7
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Paraffin
  • Proximity Ligation Assay
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.
Publications
Read Publications using H00004176-M01.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for MCM7 Antibody (6C2)

  • CDC47 homolog
  • CDC47
  • CDC47P85MCM
  • DNA replication licensing factor MCM7
  • EC 3.6.4.12
  • homolog of S. cerevisiae Cdc47
  • MCM2
  • MCM2PNAS146
  • MCM7 minichromosome maintenance deficient 7 (S. cerevisiae)
  • MCM7
  • minichromosome maintenance complex component 7
  • minichromosome maintenance deficient (S. cerevisiae) 7
  • minichromosome maintenance deficient 7
  • P1.1-MCM3
  • P1CDC47
  • P85MCM
  • PNAS146
  • PPP1R104
  • Protein Phosphatase 1

Background

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MCM7 Antibody (H00004176-M01)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol MCM7
Entrez
OMIM
Uniprot