MMR/CD206/Mannose Receptor Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse liver tissue. Gels were loaded with 12 µg, 6.5 µg, and 3 µg of tissue lysate. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified ...read more
MMR/CD206 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 5 µg/mL overnight at 4 °C. Tissue was ...read more
MMR/CD206 was detected in perfusion fixed frozen sections of mouse lung using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 25 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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MMR/CD206/Mannose Receptor Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MMR/CD206
Leu19-Ala1388
Accession # Q2HZ94
Specificity
Detects mouse MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs, less than 45% cross-reactivity with recombinant human MMR is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MRC1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF2535 in the following applications:

Publications
Read Publications using
AF2535 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMR/CD206/Mannose Receptor Antibody [Unconjugated]

  • CD206
  • CLEC13D
  • CLEC13Dmacrophage mannose receptor 1
  • C-type lectin domain family 13 member D
  • mannose receptor, C type 1
  • MMR
  • MMRCD206 antigen
  • MRC1

Background

The mouse Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 175 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The mouse MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains a 19 aa signal sequence, a 1369 aa extracellular region, a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (4). Its extracellular region is composed of an N-terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3‑5). Mouse to human, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N-acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (6). Several of the CRDs participate in the Ca2+-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (7). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor recycles to the cell surface (3, 8). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

  1. East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364. 
  2. Chieppa, M. et al. (2003) J. Immunol. 171:4552. 
  3. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77. 
  4. Harris, N. et al. (1992) Blood 80:2363.
  5. Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
  6. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
  7. Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
  8. Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMR/CD206/Mannose Receptor Antibody (AF2535)(55)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 7 applications: Flow Cytometry, ICC, IF, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
Flow Cytometry
(4)
ICC
(8)
IF
(1)
IHC
(24)
IHC-Fr
(10)
IHC-P
(3)
Western Blot
(5)
All Applications
Filter By Species
Human
(1)
Mouse
(50)
Rat
(4)
All Species
Showing Publications 1 - 10 of 55. Show All 55 Publications.
Publications using AF2535 Applications Species
Y Geng, J Yang, X Cheng, Y Han, F Yan, C Wang, X Jiang, X Meng, M Fan, M Zhao, L Zhu A bioactive gypenoside (GP-14) alleviates neuroinflammation and blood brain barrier (BBB) disruption by inhibiting the NF-kappaB signaling pathway in a mouse high-altitude cerebral edema (HACE) model International immunopharmacology, 2022;107(0):108675. 2022 [PMID: 35299003] (IHC, Mouse) IHC Mouse
J Zhang, J Ma, RT Trinh, CJ Heijnen, A Kavelaars An HDAC6 inhibitor reverses chemotherapy-induced mechanical hypersensitivity via an IL-10 and macrophage dependent pathway Brain, Behavior, and Immunity, 2021;100(0):287-296. 2021 [PMID: 34915156] (IHC, Mouse) IHC Mouse
M Seitz, C Köster, M Dzietko, H Sabir, M Serdar, U Felderhoff, I Bendix, J Herz Hypothermia modulates myeloid cell polarization in neonatal hypoxic-ischemic brain injury Journal of Neuroinflammation, 2021;18(1):266. 2021 [PMID: 34772426] (IHC, Mouse) IHC Mouse
X Ma, C Qin, M Chen, HH Yu, YH Chu, TJ Chen, DB Bosco, LJ Wu, BT Bu, W Wang, DS Tian Regulatory T cells protect against brain damage by alleviating inflammatory response in neuromyelitis optica spectrum disorder Journal of Neuroinflammation, 2021;18(1):201. 2021 [PMID: 34526069] (Western Blot, Mouse) Western Blot Mouse
X Zhou, YN Zhang, FF Li, Z Zhang, LY Cui, HY He, X Yan, WB He, HS Sun, ZP Feng, SF Chu, NH Chen Neuronal chemokine-like-factor 1 (CKLF1) up-regulation promotes M1 polarization of microglia in rat brain after stroke Acta pharmacologica Sinica, 2021;0(0):. 2021 [PMID: 34385606] (IHC, Rat) IHC Rat
M Fremder, SW Kim, A Khamaysi, L Shimshilas, H Eini-Rider, IS Park, U Hadad, JH Cheon, E Ohana A transepithelial pathway delivers succinate to macrophages, thus perpetuating their pro-inflammatory metabolic state Cell Reports, 2021;36(6):109521. 2021 [PMID: 34380041] (Western Blot, Mouse) Western Blot Mouse
L Zhang, J Chen, L Yan, Q He, H Xie, M Chen Resveratrol Ameliorates Cardiac Remodeling in a Murine Model of Heart Failure With Preserved Ejection Fraction Frontiers in Pharmacology, 2021;12(0):646240. 2021 [PMID: 34177571] (IHC, Mouse) IHC Mouse
X Xu, W Gao, L Li, J Hao, B Yang, T Wang, L Li, X Bai, F Li, H Ren, M Zhang, L Zhang, J Wang, D Wang, J Zhang, L Jiao Annexin A1 protects against cerebral ischemia-reperfusion injury by modulating microglia/macrophage polarization via FPR2/ALX-dependent AMPK-mTOR pathway Journal of Neuroinflammation, 2021;18(1):119. 2021 [PMID: 34022892] (ICC, Mouse) ICC Mouse
Z Germana, G Simona, L Marialucia, M Biagina, V Christine, F Paola, C Matteo, C Pasquale, M Davide, T Mario, M Massimilia, G Carlo, S Gaia, M Fabio Hypoxia-induced miR-210 modulates the inflammatory response and fibrosis upon acute ischemia Cell Death & Disease, 2021;12(5):435. 2021 [PMID: 33934122] (IHC, Mouse) IHC Mouse
F Garello, M Boido, M Miglietti, V Bitonto, M Zenzola, M Filippi, F Arena, L Consolino, M Ghibaudi, E Terreno Imaging of Inflammation in Spinal Cord Injury: Novel Insights on the Usage of PFC-Based Contrast Agents Biomedicines, 2021;9(4):. 2021 [PMID: 33916774] (ICC, Mouse) ICC Mouse
Show All 55 Publications.

Reviews for MMR/CD206/Mannose Receptor Antibody (AF2535) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Mouse and Rat.

Reviews using AF2535:
Filter by Applications
IHC-P
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Mouse
(1)
Mouse and Rat
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin MMR/CD206/Mannose Receptor AF2535
Enlarge
4
reviewed by:
Imke Jansen
IHC-P Mouse and Rat 03/16/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedTestis
SpeciesMouse and Rat
LotWFT0118011

Comments

CommentsMouse and Rat Testis: 4% Neutral buffered Formalin fixated for 3 days and subsequently dehydrated in alcohol steps (2x 70%, 2x 96%, 2x 100%) xyleen and paraffin embedded.

Primary antibodies:
1/100 dilution     MMR/CD206 Goat polyclonal IgG      AF2535
Lot#WFT0118011
1/500 dilution     Goat IgG isotype 10 ug/ml     02-6202
Lot#TA262200
both working concentration: 10 ug/mL

Since rat species have not been tested for MMR/CD206 antibody (AF2535), we decided to take part in the Innovator’s Reward™ program.
MMR/CD206 was already detected in perfusion fixed frozen sections of mouse testis with specific staining localized to spermatocytes in testis. Therefore, we included mouse testis (positive control) and rat testis tissue, that is formalin fixated and paraffin embedded.
  4
reviewed by:
Verified Customer
IHC-Fr Mouse 01/07/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23942366
SpeciesMouse

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Recent Reviews

4
5
0
4
2
3
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2
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1
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Imke Jansen
03/16/2018
Application: IHC-P
Species: Mouse and Rat

 
Verified Customer
01/07/2015
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol MRC1
Uniprot