MMR/CD206/Mannose Receptor Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse liver tissue. Gels were loaded with 12 µg, 6.5 µg, and 3 µg of tissue lysate. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified ...read more
MMR/CD206 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 5 µg/mL overnight at 4 °C. Tissue was ...read more
MMR/CD206 was detected in perfusion fixed frozen sections of mouse lung using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 25 µg/mL overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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View Available Formulations

MMR/CD206/Mannose Receptor Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse MMR/CD206
Leu19-Ala1388
Accession # Q2HZ94
Specificity
Detects mouse MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs, less than 45% cross-reactivity with recombinant human MMR is observed.
Details of Functionality
ActivityAssaywCitation not found None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
MRC1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
Reviewed Applications
Read 2 Reviews rated 4
using
AF2535 in the following applications:

Publications
Read Publications using
AF2535 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMR/CD206/Mannose Receptor Antibody [Unconjugated]

  • CD206
  • CLEC13D
  • CLEC13Dmacrophage mannose receptor 1
  • C-type lectin domain family 13 member D
  • mannose receptor, C type 1
  • MMR
  • MMRCD206 antigen
  • MRC1

Background

The mouse Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 175 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The mouse MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains a 19 aa signal sequence, a 1369 aa extracellular region, a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (4). Its extracellular region is composed of an N-terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3‑5). Mouse to human, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N-acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (6). Several of the CRDs participate in the Ca2+-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (7). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor recycles to the cell surface (3, 8). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

  1. East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364. 
  2. Chieppa, M. et al. (2003) J. Immunol. 171:4552. 
  3. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77. 
  4. Harris, N. et al. (1992) Blood 80:2363.
  5. Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
  6. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
  7. Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
  8. Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MMR/CD206/Mannose Receptor Antibody (AF2535)(25)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 6 applications: Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Western Blot.


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Flow Cytometry
(3)
ICC
(3)
IHC
(8)
IHC-Fr
(7)
IHC-P
(2)
Western Blot
(2)
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Filter By Species
Mouse
(23)
Rat
(2)
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Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using AF2535 Applications Species
T Koizumi, K Taguchi, I Mizuta, H Toba, M Ohigashi, O Onishi, K Ikoma, S Miyata, T Nakata, M Tanaka, S Foulquier, HWM Steinbusch, T Mizuno Transiently proliferating perivascular microglia harbor M1 type and precede cerebrovascular changes in a chronic hypertension model J Neuroinflammation, 2019;16(1):79. 2019 [PMID: 30971251] (IHC, Rat) IHC Rat
S Morita-Tak, K Nakahara, S Hasegawa-I, A Isonishi, K Tatsumi, H Okuda, T Tanaka, M Kitabatake, T Ito, A Wanaka Responses of perivascular macrophages to circulating lipopolysaccharides in the subfornical organ with special reference to endotoxin tolerance J Neuroinflammation, 2019;16(1):39. 2019 [PMID: 30764851] (IHC-Fr, Mouse) IHC-Fr Mouse
V Bellver-La, F Bretheau, B Mailhot, N Vallières, M Lessard, ME Janelle, N Vernoux, MÈ Tremblay, T Fuehrmann, MS Shoichet, S Lacroix Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury Nat Commun, 2019;10(1):518. 2019 [PMID: 30705270] (IHC, Mouse) IHC Mouse
C Qin, Q Liu, ZW Hu, LQ Zhou, K Shang, DB Bosco, LJ Wu, DS Tian, W Wang Microglial TLR4-dependent autophagy induces ischemic white matter damage via STAT1/6 pathway Theranostics, 2018;8(19):5434-5451. 2018 [PMID: 30555556] (ICC, Mouse) ICC Mouse
J Ye, Y Huang, B Que, C Chang, W Liu, H Hu, L Liu, Y Shi, Y Wang, M Wang, T Zeng, W Zhen, Y Xu, L Shi, J Liu, H Jiang, D Ye, Y Lin, J Wan, Q Ji Interleukin-12p35 Knock Out Aggravates Doxorubicin-Induced Cardiac Injury and Dysfunction by Aggravating the Inflammatory Response, Oxidative Stress, Apoptosis and Autophagy in Mice EBioMedicine, 2018;35(0):29-39. 2018 [PMID: 30228093] (IHC, Mouse) IHC Mouse
K Hashimoto, M Nakashima, A Hamano, M Gotoh, H Ikeshima-K, K Murakami-M, Y Miyamoto 2-carba cyclic phosphatidic acid suppresses inflammation via regulation of microglial polarisation in the stab-wounded mouse cerebral cortex Sci Rep, 2018;8(1):9715. 2018 [PMID: 29946114] (ICC, Mouse) ICC Mouse
JA Lindborg, JP Niemi, MA Howarth, KW Liu, CZ Moore, D Mahajan, RE Zigmond Molecular and cellular identification of the immune response in peripheral ganglia following nerve injury J Neuroinflammation, 2018;15(1):192. 2018 [PMID: 29945607] (IHC-Fr, Mouse) IHC-Fr Mouse
G Liu, P Lu, L Chen, W Zhang, M Wang, D Li, X Zhang B-cell leukemia/lymphoma 10 promotes angiogenesis in an experimental corneal neovascularization model Eye (Lond), 2018;0(0):. 2018 [PMID: 29515217] (Flow Cytometry, Mouse) Flow Cytometry Mouse
S Kageyama, K Nakamura, B Ke, RW Busuttil, JW Kupiec-Weg Serelaxin Induces Notch1 Signaling and Alleviates Hepatocellular Damage in Orthotopic Liver Transplantation Am. J. Transplant., 2018;0(0):. 2018 [PMID: 29464890] (Western Blot, Mouse) Western Blot Mouse
X Liu, S Wen, F Yan, K Liu, L Liu, L Wang, S Zhao, X Ji Salidroside provides neuroprotection by modulating microglial polarization after cerebral ischemia J Neuroinflammation, 2018;15(1):39. 2018 [PMID: 29426336] (ICC, Mouse) ICC Mouse
Show All 25 Publications.

Reviews for MMR/CD206/Mannose Receptor Antibody (AF2535) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat and Mouse.

Reviews using AF2535:
Filter by Applications
IHC-P
(1)
IHC-Fr
(1)
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Mouse
(1)
Rat and Mouse
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin MMR/CD206/Mannose Receptor AF2535
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reviewed by:
Imke Jansen
IHC-P Rat and Mouse 03/16/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedTestis
SpeciesRat and Mouse
LotWFT0118011

Comments

CommentsMouse and Rat Testis: 4% Neutral buffered Formalin fixated for 3 days and subsequently dehydrated in alcohol steps (2x 70%, 2x 96%, 2x 100%) xyleen and paraffin embedded.

Primary antibodies:
1/100 dilution     MMR/CD206 Goat polyclonal IgG      AF2535
Lot#WFT0118011
1/500 dilution     Goat IgG isotype 10 ug/ml     02-6202
Lot#TA262200
both working concentration: 10 ug/mL

Since rat species have not been tested for MMR/CD206 antibody (AF2535), we decided to take part in the Innovator’s Reward™ program.
MMR/CD206 was already detected in perfusion fixed frozen sections of mouse testis with specific staining localized to spermatocytes in testis. Therefore, we included mouse testis (positive control) and rat testis tissue, that is formalin fixated and paraffin embedded.
 
reviewed by:
Anonymous
IHC-Fr Mouse 01/07/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 23942366
SpeciesMouse

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FAQs for MMR/CD206/Mannose Receptor Antibody (AF2535) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 FAB2535G
Allophycocyanin FAB2535A
Biotin BAF2535
Phycoerythrin FAB2535P

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Imke Jansen
03/16/2018
Application: IHC-P
Species: Rat and Mouse

 
Anonymous
01/07/2015
Application: IHC-Fr
Species: Mouse

Bioinformatics

Gene Symbol MRC1
Uniprot