MMR/CD206/Mannose Receptor Antibody [Unconjugated]

Western blot shows lysates of mouse liver tissue. Gels were loaded with 12 µg, 6.5 µg, and 3 µg of tissue lysate. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MMR/CD206 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MMR/CD206 was detected in perfusion fixed frozen sections of mouse testis using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to spermatocytes in testis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

MMR/CD206 was detected in perfusion fixed frozen sections of mouse lung using Goat Anti-Mouse MMR/CD206 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2535) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of macrophages. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Cells of human meninges co-express LLEC markers. a–c DAB-IHC with single antibodies detects VEGFR3 (a), LYVE1 (b), and MRC1 (c) in the meninges of human post mortem brain showing no signs of neuropathology. These images are taken from a 38 year old male (sample P17/07, Table 1), and confirmed in n = 2 additional samples. P parenchyma. Scale = 150 µm (a); 40 µm (b); and 20 µm (c). d–f DAB-IHC with single antibodies detects VEGFR3 (b), LYVE1 (c), and MRC1 (d) in elderly human meninges (age: 89–92) with evidence of neuropathology and confirmed in n = 3 brains (Table 1). P, parenchyma. Scale = 20 µm. g–p IHC with fluorescent antibodies detects human meningeal cells that co-express MRC1 (h, m, yellow), LYVE1 (i, n, white), and VEGFR3 (j, o, green). Nuclei/RNA are labelled with DAPI (g, l, blue) and images are merged in (k, p). Scale = 10 µm Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by R&D Systems.

M1 and M2 phenotype in spinal cord after intraplantar IL-1 beta . Wild-type (WT) and LysM-G protein–coupled receptor kinase (GRK)2+/− mice received an intraplantar injection of 1 ng IL-1 beta . At 15 hours after injection, spinal cord was collected, and frozen sections of (A) lumbar spinal cord (L2 to L5) and as control (B) thoracic spinal cord (T6 to T10) were stained for M1 (CD16/32) and M2 (CD206 and arginase-I) phenotypic markers. A representative example of M1 and M2 staining in the dorsal horn of one of the four mice per group is displayed. Scale bar indicates 20 μm. (C) Quantification of microglia/macrophages expressing M1 and M2 phenotypic markers in spinal cord from WT and LysM-GRK2+/− mice. Expression was quantified in approximately 10 to 15 dorsal horns of spinal cords per group (4 mice per group). The level of expression in the lumbar or thoracic area from control WT mice was set at 100%. Data are expressed as means ± SEM. **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22731384), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunofluorescent staining for macrophage marker F4/80 in Acomys and Mus.(A–C) Bone-marrow-derived cells isolated from Acomys and stained for F4/80 (green). (A) unstimulated cells, (B) cells stimulated with IFN gamma and LPS, (C) cells stimulated with IL-4. (D–F) Bone-marrow-derived cells isolated from Mus and stained for F4/80 (green). (D) unstimulated cells, (E) cells stimulated with IFN gamma and LPS, and (F) cells stimulated with IL-4. Scale bar = 50 μm. (G) Acomys ear tissue at D15 after injury stained for F4/80 (green), CD206 (red) and DAPI (grey). (H) Mus ear tissue at D7 after injury stained for F4/80 (green), CD206 (red) and DAPI (grey). Scale bar = 50 μm.DOI://dx.doi.org/10.7554/eLife.24623.012 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28508748), licensed under a CC-BY license. Not internally tested by R&D Systems.

Characterization of M2 BV2 cells induced by IL-4 and identification of sEVs derived from M2 BV2 cells. (A, B) Representative images of BV2 cells immunostained for Iba-1 (green), CD206 (red), and arginase (red). Cultured systems were treated with 0 or 20 ng/µL IL-4. Cell nuclei were counterstained with DAPI. Scale bar = 50 µm. (C) Western blotting analysis of CD206 and arginase expression in BV2 cells after 0 or 20 ng/µL IL-4 treatment. (D) Representative electron microscopy images showing the phenotype of M2-sEVs. Left image scale bar = 100 nm, right image scale bar = 50 nm. (F) NTA of M2-sEVs isolated by ultracentrifugation from M2 BV2 cells. Data represent the average size distribution profile of three samples and each purification normalized to the total nanoparticle concentrations. Data for each sample was derived from three different videos and analyses. (G) Western blotting analysis of TSG101 and CD63 levels in M2 BV2 cells and M2-sEVs. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33391532), licensed under a CC-BY license. Not internally tested by R&D Systems.

Cells of human meninges co-express LLEC markers. a–c DAB-IHC with single antibodies detects VEGFR3 (a), LYVE1 (b), and MRC1 (c) in the meninges of human post mortem brain showing no signs of neuropathology. These images are taken from a 38 year old male (sample P17/07, Table 1), and confirmed in n = 2 additional samples. P parenchyma. Scale = 150 µm (a); 40 µm (b); and 20 µm (c). d–f DAB-IHC with single antibodies detects VEGFR3 (b), LYVE1 (c), and MRC1 (d) in elderly human meninges (age: 89–92) with evidence of neuropathology and confirmed in n = 3 brains (Table 1). P, parenchyma. Scale = 20 µm. g–p IHC with fluorescent antibodies detects human meningeal cells that co-express MRC1 (h, m, yellow), LYVE1 (i, n, white), and VEGFR3 (j, o, green). Nuclei/RNA are labelled with DAPI (g, l, blue) and images are merged in (k, p). Scale = 10 µm Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by R&D Systems.

Mouse LLECs take up A beta 1-40. a Schematic showing the site of dye and A beta 1-40 perfusion into the CSF via the cisterna magna (arrow) of a 2-month old mouse. The dotted line indicates the plane of section. A anterior, P posterior, D dorsal, V ventral. b Coronal brain section indicating the areas imaged. SF4 refers to area captured in Figure S4. c The percentage of each labelled cell type that internalized perfused A beta . Cells co-expressing VEGFR3 and LYVE1 take up A beta at a higher rate than MRC1, LYVE1 double-positive cells as well as MRC1-positive, LYVE1-negative cells (p ≤ 0.05, bootstrap). VEGFR3, LYVE1 counts, n = 2 brains (3 sections/brain). MRC1, LYVE1 counts, n = 3 brains (3 sections/brain). d–d′′′ Cells of the adult mouse meninges that co-express VEGFR3 (d, green) and LYVE1 (d′, white) internalize A beta 1-40 (d′′, cyan). Scale = 20 µm. e-e′′′) Cells of the adult mouse meninges that co-express VEGFR3 (e, green) and MRC1 (e′, white) internalize A beta 1-40 (e′′, cyan). Scale = 40 µm. f–f′′′) Cells of the adult mouse meninges that co-express MRC1 (f, magenta) and LYVE1 (f′, white) internalize A beta 1-40 (f′′, cyan). The walls of a blood vessel (white arrowhead, f′′) also accumulate A beta 1-40. Scale = 60 µm Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by R&D Systems.

Cells of human meninges co-express LLEC markers. a–c DAB-IHC with single antibodies detects VEGFR3 (a), LYVE1 (b), and MRC1 (c) in the meninges of human post mortem brain showing no signs of neuropathology. These images are taken from a 38 year old male (sample P17/07, Table 1), and confirmed in n = 2 additional samples. P parenchyma. Scale = 150 µm (a); 40 µm (b); and 20 µm (c). d–f DAB-IHC with single antibodies detects VEGFR3 (b), LYVE1 (c), and MRC1 (d) in elderly human meninges (age: 89–92) with evidence of neuropathology and confirmed in n = 3 brains (Table 1). P, parenchyma. Scale = 20 µm. g–p IHC with fluorescent antibodies detects human meningeal cells that co-express MRC1 (h, m, yellow), LYVE1 (i, n, white), and VEGFR3 (j, o, green). Nuclei/RNA are labelled with DAPI (g, l, blue) and images are merged in (k, p). Scale = 10 µm Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunofluorescent staining for macrophage marker F4/80 in Acomys and Mus.(A–C) Bone-marrow-derived cells isolated from Acomys and stained for F4/80 (green). (A) unstimulated cells, (B) cells stimulated with IFN gamma and LPS, (C) cells stimulated with IL-4. (D–F) Bone-marrow-derived cells isolated from Mus and stained for F4/80 (green). (D) unstimulated cells, (E) cells stimulated with IFN gamma and LPS, and (F) cells stimulated with IL-4. Scale bar = 50 μm. (G) Acomys ear tissue at D15 after injury stained for F4/80 (green), CD206 (red) and DAPI (grey). (H) Mus ear tissue at D7 after injury stained for F4/80 (green), CD206 (red) and DAPI (grey). Scale bar = 50 μm.DOI://dx.doi.org/10.7554/eLife.24623.012 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28508748), licensed under a CC-BY license. Not internally tested by R&D Systems.

Mouse LLECs take up A beta 1-40. a Schematic showing the site of dye and A beta 1-40 perfusion into the CSF via the cisterna magna (arrow) of a 2-month old mouse. The dotted line indicates the plane of section. A anterior, P posterior, D dorsal, V ventral. b Coronal brain section indicating the areas imaged. SF4 refers to area captured in Figure S4. c The percentage of each labelled cell type that internalized perfused A beta . Cells co-expressing VEGFR3 and LYVE1 take up A beta at a higher rate than MRC1, LYVE1 double-positive cells as well as MRC1-positive, LYVE1-negative cells (p ≤ 0.05, bootstrap). VEGFR3, LYVE1 counts, n = 2 brains (3 sections/brain). MRC1, LYVE1 counts, n = 3 brains (3 sections/brain). d–d′′′ Cells of the adult mouse meninges that co-express VEGFR3 (d, green) and LYVE1 (d′, white) internalize A beta 1-40 (d′′, cyan). Scale = 20 µm. e-e′′′) Cells of the adult mouse meninges that co-express VEGFR3 (e, green) and MRC1 (e′, white) internalize A beta 1-40 (e′′, cyan). Scale = 40 µm. f–f′′′) Cells of the adult mouse meninges that co-express MRC1 (f, magenta) and LYVE1 (f′, white) internalize A beta 1-40 (f′′, cyan). The walls of a blood vessel (white arrowhead, f′′) also accumulate A beta 1-40. Scale = 60 µm Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by R&D Systems.

In vitro activation assays shows Acomys macrophages can be polarized to express different markers.(A–I) Bone-marrow-derived macrophages isolated from Acomys femurs are cultured with no cytokines (unstimulated, A, D, G) with IFN gamma +LPS (M1, B, E, H) or with IL-4 (M2, C, F, I). Immunocytochemistry for the pan-macrophage marker CD11b (green) (A–C), for the M1 macrophage marker CD86 (green) and the M2 macrophage marker Arginase 1 (red) (D–F), or CD206 (red) (G–I). (J–R). Bone-marrow-derived macrophages were isolated from Mus femurs and cultured with no cytokines (J, M, P) with IFN gamma and LPS (K, N, Q) or with IL4 (L, O, R) as above. Immunocytochemistry was performed for CD11b (green) (J–K), for CD86 (green) and Arginase 1 (red) (M–O), and CD206 (red) (P–R). Nuclei were counterstained with DAPI (grey) in all panels. Scale bars = 50 μm. Images are representative of n = 3 technical replicates.DOI://dx.doi.org/10.7554/eLife.24623.011Immunofluorescent staining for macrophage marker F4/80 in Acomys and Mus.(A–C) Bone-marrow-derived cells isolated from Acomys and stained for F4/80 (green). (A) unstimulated cells, (B) cells stimulated with IFN gamma and LPS, (C) cells stimulated with IL-4. (D–F) Bone-marrow-derived cells isolated from Mus and stained for F4/80 (green). (D) unstimulated cells, (E) cells stimulated with IFN gamma and LPS, and (F) cells stimulated with IL-4. Scale bar = 50 μm. (G) Acomys ear tissue at D15 after injury stained for F4/80 (green), CD206 (red) and DAPI (grey). (H) Mus ear tissue at D7 after injury stained for F4/80 (green), CD206 (red) and DAPI (grey). Scale bar = 50 μm.DOI://dx.doi.org/10.7554/eLife.24623.012 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28508748), licensed under a CC-BY license. Not internally tested by R&D Systems.

Microglial activation was attenuated by Hv1 deletion following LPC-induced demyelination. a Representative images of Iba-1 immunostaining in the CC of WT and Hv1−/− mice (scale bar, 200 μm). b Quantification of the number of microglia per high-power field (HPF) in the CC. Each point of WT and Hv1−/− mice, N = 5-7 mice. c Representative images of Iba-1 morphology and the corresponding 3D reconstructions (scale bars, magnified images, 20 μm; 3D reconstruction images, 5um) d Quantification analysis of the soma of microglia. Each point of WT and Hv1−/− mice, N = 4-6 mice, 6-15 cells per mouse. e Representative images of Iba-1 and CD16/32 co-localization in the CC of WT and Hv1−/− mice (scale bar, 50 μm; magnified images, 20 μm). f Quantification of the ratio of CD16/32+/Iba-1+. Each point of WT mice, N = 5-8 mice; Hv1−/− mice, N = 5-8 mice. g Representative images of Iba-1 and CD206 co-localization in the CC of WT and Hv1−/− mice (scale bar, 50 μm; magnified images, 20 μm). h Quantification of the ratio of CD206+/Iba-1+. Each point of WT mice, N = 6-7 mice; Hv1−/− mice, N = 5-6 mice. Data are shown as mean ± SD, *P < 0.05, ***P<0.001, two-way ANOVA with Dunnett’s post hoc test Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33158440), licensed under a CC-BY license. Not internally tested by R&D Systems.

Cells with BLEC molecular markers are present within the mouse leptomeninges. a Coronal brain section of adult zebrafish brain indicating the imaging area in the dorsal optic tectum (TeO). b A 14 month old Tg(kdr-l:mCherry); Tg(flt4:mCitrine) double transgenic zebrafish has cells in the meninges (white bracket) that express flt4/vegfr3 ( alpha -GFP, green) near kdr-l positive ( alpha -RFP, red) blood vessels. DAPI (blue) labels the nuclei. Scale = 50 µm. c Coronal mouse brain section showing the imaging areas of the meninges. d As revealed by IHC, 17-week-old mouse brains express VEGFR3 (green) in the meninges (white bracket). Tie2-GFP;NG2-DsRed double reporter mice were used to distinguish arteries and veins. NG2 (red) labels pericytes and smooth muscle cells, Tie2 (magenta) labels vascular endothelial cells, and Hoechst (blue) stains nuclei. The image is rotated with the parenchyma at the bottom for ease of comparison with panel b. Scale = 50 µm. e-e′′′ As revealed by IHC, cells of the meninges co-express MRC1 (e, yellow), LYVE1 (e′, white), and VEGFR3 (e′′, green). Red arrows highlight cells expressing these three markers. The images are rotated with the parenchyma at the bottom. scale = 30 µm. f, g Quantification of the relative numbers of single and double-labelled cells in 2-month old mouse meninges. VEGFR3 and LYVE1 cell counts were from n = 2 brains, 3 coronal sections (10 area images)/brain. MRC1 and LYVE1 cell counts were from n = 3 brains, 3 coronal sections (4 area images)/brain. The mean values for each set are depicted Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by R&D Systems.

M1 and M2 phenotype in spinal cord after intraplantar IL-1 beta . Wild-type (WT) and LysM-G protein–coupled receptor kinase (GRK)2+/− mice received an intraplantar injection of 1 ng IL-1 beta . At 15 hours after injection, spinal cord was collected, and frozen sections of (A) lumbar spinal cord (L2 to L5) and as control (B) thoracic spinal cord (T6 to T10) were stained for M1 (CD16/32) and M2 (CD206 and arginase-I) phenotypic markers. A representative example of M1 and M2 staining in the dorsal horn of one of the four mice per group is displayed. Scale bar indicates 20 μm. (C) Quantification of microglia/macrophages expressing M1 and M2 phenotypic markers in spinal cord from WT and LysM-GRK2+/− mice. Expression was quantified in approximately 10 to 15 dorsal horns of spinal cords per group (4 mice per group). The level of expression in the lumbar or thoracic area from control WT mice was set at 100%. Data are expressed as means ± SEM. **P < 0.01, ***P < 0.001. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/22731384), licensed under a CC-BY license. Not internally tested by R&D Systems.

Cells of human meninges co-express LLEC markers. a–c DAB-IHC with single antibodies detects VEGFR3 (a), LYVE1 (b), and MRC1 (c) in the meninges of human post mortem brain showing no signs of neuropathology. These images are taken from a 38 year old male (sample P17/07, Table 1), and confirmed in n = 2 additional samples. P parenchyma. Scale = 150 µm (a); 40 µm (b); and 20 µm (c). d–f DAB-IHC with single antibodies detects VEGFR3 (b), LYVE1 (c), and MRC1 (d) in elderly human meninges (age: 89–92) with evidence of neuropathology and confirmed in n = 3 brains (Table 1). P, parenchyma. Scale = 20 µm. g–p IHC with fluorescent antibodies detects human meningeal cells that co-express MRC1 (h, m, yellow), LYVE1 (i, n, white), and VEGFR3 (j, o, green). Nuclei/RNA are labelled with DAPI (g, l, blue) and images are merged in (k, p). Scale = 10 µm Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31696318), licensed under a CC-BY license. Not internally tested by R&D Systems.

Representative images of HE, M3/84 (macrophages) and MR (CD206) immunohistochemistry. In control mice, no plaques with macrophages were observed, while fibrous/fibroatheromatous plaques were present in the aortas extracted from ApoE-KO mice. The lesions (fatty streaks and fibrous plaques) showed high amounts of MR+ macrophages (100 μm (bars), vascular lumen (L), intima (arrow), media (asterisk), adventitia (arrowhead)) Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28470406), licensed under a CC-BY license. Not internally tested by R&D Systems.

TAMs along the beam paths show a mixed M2-like/M1-like phenotype and expression of phagocytosis markers. (A) Double staining for CD68 (red) and CD206 (green) plus DAPI (blue) shows at 7 days post-MRT that many of the underlined TAMs are positive for both markers, indicating their inclination towards an M2-like phenotype. Dashed white lines demarcate the border between the tumour (lower part) and the normal tissue (upper part). (B) Triple staining for CD68 (red), CD206 (green) and Dectin-1 (grey) shows an abundant triple positive TAM population along the beam paths, indicating their inclination towards a phagocytic phenotype. (C) Double staining for CD68 (red) and Ly6C (cyan) reveals a partial M1-like TAM population and, at the same time, reveals cells that are not double positive, indicating the presence of recruited monocytes. Dashed yellow lines indicate a macrophage cluster along a microbeam path. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35453485), licensed under a CC-BY license. Not internally tested by R&D Systems.

Hypothermia promotes a shift of microglia/macrophages towards an anti‐inflammatory phenotype in aged female mice 7 days after ischemic stroke. Mice were subjected to distal MCAO (dMCAO) or sham surgery. Normothermia (NT) or hypothermia (HT) was induced for 50 min immediately after dMCAO. Coronal brain sections were stained for Iba‐1 (a microglial/macrophage marker) and CD206 (an anti‐inflammatory marker) or CD16 (a pro‐inflammatory marker). (A) Representative images of Iba1/CD16 and Iba1/CD206 immunofluorescence staining in the ipsilateral peri‐infarct cortex (CTX) regions. (B) Representative images showing Iba1/CD16 and Iba1/CD206 immunofluorescence staining in the ipsilateral peri‐infarct striatum (STR) regions. (C) Representative magnified 3‐dimensional images of Iba1/CD16 and Iba1/CD206 staining. (D) Quantification of the total number of Iba1+ cells (upper panel), Iba1+/CD16+ cells (middle panel), and Iba1+/CD206+ cells (lower panel). The number of double‐positive cells was expressed as the number over 100 Iba1+ cells. n = 5 for sham (S), n = 6 for NT and n = 6 for HT. (E) Pearson correlation analysis of NeuN+ cells with Iba1+/CD206+ cells in the CTX region, n = 6 per group. (F) Pearson correlation analysis of beta ‐APP+ cells with Iba1+/CD206+ cells in the CTX region, n = 6 per group. Scale bar: 50 μm. Data are shown as mean ± SD. *p < 0.05, **p < 0.01 NT vs. HT, ns, no significance. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36341958), licensed under a CC-BY license. Not internally tested by R&D Systems.

HCK knockout reduced kidney fibrosis in uIRIx model with regulation of macrophage activation.A Schematic diagram of strategy of uIRIx models. B Urine ACR and serum BUN for WT and HCK KO uIRIx mice. C Representative images of H&E, COL1A1 IF and Masson trichrome staining from WT and KO uIRIx kidneys. Morphometric quantification (n = 5 animals; 5 random fields/animal) of the COL1A1 (top) and Masson (lower) positive area. D Representative IF staining images and quantification of from kidneys of HCK and F4/80 macrophage from WT and HCK KO uIRIx kidneys. E Western blot and quantification of M1 and M2 macrophage markers from WT and HCK KO mice kidneys at 28 days post uIRIx. F Representative IF staining images and quantification of LC3 in macrophages from WT & HCK KO uIRIx kidneys. B, C and E*p < 0.05 with t test. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37463911), licensed under a CC-BY license. Not internally tested by R&D Systems.

Hypothermia promotes a shift of microglia/macrophages towards an anti‐inflammatory phenotype in aged female mice 7 days after ischemic stroke. Mice were subjected to distal MCAO (dMCAO) or sham surgery. Normothermia (NT) or hypothermia (HT) was induced for 50 min immediately after dMCAO. Coronal brain sections were stained for Iba‐1 (a microglial/macrophage marker) and CD206 (an anti‐inflammatory marker) or CD16 (a pro‐inflammatory marker). (A) Representative images of Iba1/CD16 and Iba1/CD206 immunofluorescence staining in the ipsilateral peri‐infarct cortex (CTX) regions. (B) Representative images showing Iba1/CD16 and Iba1/CD206 immunofluorescence staining in the ipsilateral peri‐infarct striatum (STR) regions. (C) Representative magnified 3‐dimensional images of Iba1/CD16 and Iba1/CD206 staining. (D) Quantification of the total number of Iba1+ cells (upper panel), Iba1+/CD16+ cells (middle panel), and Iba1+/CD206+ cells (lower panel). The number of double‐positive cells was expressed as the number over 100 Iba1+ cells. n = 5 for sham (S), n = 6 for NT and n = 6 for HT. (E) Pearson correlation analysis of NeuN+ cells with Iba1+/CD206+ cells in the CTX region, n = 6 per group. (F) Pearson correlation analysis of beta ‐APP+ cells with Iba1+/CD206+ cells in the CTX region, n = 6 per group. Scale bar: 50 μm. Data are shown as mean ± SD. *p < 0.05, **p < 0.01 NT vs. HT, ns, no significance. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36341958), licensed under a CC-BY license. Not internally tested by R&D Systems.

Quantification of the number of perivascular macrophages (PVMs). The number of PVMs in the cerebral cortex was counted and compared between the control and CB-NP exposure groups. (A–F) Low-magnification images to quantify the number of PVMs using MMR expression. (A,D) are merged pictures of (B,C,E,F), respectively. Arrowheads indicate MMR-positive PVMs. (G) Number of PVMs in the cerebral cortex. Values are expressed as the mean ± SD. Abbreviation: Carbon black nanoparticle (CB-NP), Cerebral cortex (Cx), Corpus callosum (CC), and Striatum (Str), Standard deviation (SD). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35391824), licensed under a CC-BY license. Not internally tested by R&D Systems.

RP-832c peptide levels significantly decreased in CD206 + macrophages and the profibrotic markers alpha -SMA and TGF-beta 1 without affecting Siglec-F-positive alveolar macrophages. (A) Representative 20× and 40× images of immunohistochemistry staining using anti-Siglec-F and anti-CD206 antibodies in lung tissues of naïve mice, as well as BLM-challenged vehicle/RP-832c-treated mice. The calibration bar represents 10 µm. (B) Representative 20× and 40× images of immunofluorescence staining of lung tissues using antibodies for alpha -SMA (red) and TGF beta -1 (green). The calibration bar represents 20 µm. (C–F) Bar graphs showing the quantifications of the IHC (Siglec-F & CD206) and IF ( alpha -SMA & TGF-beta 1) staining of lung tissues. Quantifications was done using Metamorph imaging software. S. E. **** p < 0.0001, *** p < 0.001, ** p < 0.01 and * p < 0.05 were considered significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37174654), licensed under a CC-BY license. Not internally tested by R&D Systems.

RP-832c peptide levels significantly decreased in CD206 + macrophages and the profibrotic markers alpha -SMA and TGF-beta 1 without affecting Siglec-F-positive alveolar macrophages. (A) Representative 20× and 40× images of immunohistochemistry staining using anti-Siglec-F and anti-CD206 antibodies in lung tissues of naïve mice, as well as BLM-challenged vehicle/RP-832c-treated mice. The calibration bar represents 10 µm. (B) Representative 20× and 40× images of immunofluorescence staining of lung tissues using antibodies for alpha -SMA (red) and TGF beta -1 (green). The calibration bar represents 20 µm. (C–F) Bar graphs showing the quantifications of the IHC (Siglec-F & CD206) and IF ( alpha -SMA & TGF-beta 1) staining of lung tissues. Quantifications was done using Metamorph imaging software. S. E. **** p < 0.0001, *** p < 0.001, ** p < 0.01 and * p < 0.05 were considered significant. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37174654), licensed under a CC-BY license. Not internally tested by R&D Systems.

RP-832c peptide is comparable to Pirfenidone in decreasing fibrosis in an established late model of BLM-induced lung fibrosis. (A) A schematic of the animal study in which mice were challenged with a 2.5 U/kg body weight dose of BLM at day 0. A total of 14 days post-BLM challenge, mice were treated with either a vehicle, 10 mg/kg RP-832c (QD), 30 mg/kg Pirfenidone (Q3D), or a combination of RP-832c and Pirfenidone for an additional 21 days. (B) Histological analysis of lung tissues. The upper panel shows representative 2× images of Masson’s Trichrome-stained whole lung tissue, the middle panel contains representative 20× images, and the lower panel shows 10× images representing anti-CD206 immunohistochemical staining of lung tissue. (C) Modified Ashcroft scoring of each treatment group, determined after 21 days of treatment. n = 6 per treatment group. S. E. ** p < 0.001, and * p < 0.05 were considered significant. (D) Quantification of the anti-CD206 IHC-stained lung tissues. The bar represents 10 µm. n = 6 per treatment group. S. E. **** p < 0.0001, ** p < 0.001, and * p < 0.05 were considered significant. (E) Body weights of the mice measured over the course of treatment in each group. n = 6 per treatment group. (F) Lung weights of the mice measured at the end of the study. n = 6 per treatment group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37174654), licensed under a CC-BY license. Not internally tested by R&D Systems.

RP-832c peptide is comparable to Pirfenidone in decreasing fibrosis in an established late model of BLM-induced lung fibrosis. (A) A schematic of the animal study in which mice were challenged with a 2.5 U/kg body weight dose of BLM at day 0. A total of 14 days post-BLM challenge, mice were treated with either a vehicle, 10 mg/kg RP-832c (QD), 30 mg/kg Pirfenidone (Q3D), or a combination of RP-832c and Pirfenidone for an additional 21 days. (B) Histological analysis of lung tissues. The upper panel shows representative 2× images of Masson’s Trichrome-stained whole lung tissue, the middle panel contains representative 20× images, and the lower panel shows 10× images representing anti-CD206 immunohistochemical staining of lung tissue. (C) Modified Ashcroft scoring of each treatment group, determined after 21 days of treatment. n = 6 per treatment group. S. E. ** p < 0.001, and * p < 0.05 were considered significant. (D) Quantification of the anti-CD206 IHC-stained lung tissues. The bar represents 10 µm. n = 6 per treatment group. S. E. **** p < 0.0001, ** p < 0.001, and * p < 0.05 were considered significant. (E) Body weights of the mice measured over the course of treatment in each group. n = 6 per treatment group. (F) Lung weights of the mice measured at the end of the study. n = 6 per treatment group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37174654), licensed under a CC-BY license. Not internally tested by R&D Systems.

DSS-treated TLR4-SNP mice exhibit reduced M2a markers in the colon and reduced beta -hydroxybutyrate ( beta -HB) levels in the sera. (A) M2a gene expression measured in the MC of male WT vs TLR4-SNP mice, comparing control, Day 9, and Day 11 samples by qRT-PCR. One-way ANOVA with Sidak multiple comparisons test. *P < 0.05; **P < 0.01; ****P < 0.0001. Data are derived from two independent experiments. In total, control WT n = 11; control TLR4-SNP n = 15; WT Day 9 n = 13, Day 11 n = 13; TLR4-SNP Day 9 n = 15, Day 11 n = 15. (B) M2a (Ym1, Arg1) protein production measured in DC samples in WT vs TLR4-SNP mice, comparing control and Day 11 samples. Data are representative of results from two independent experiments totaling control WT n = 5; control TLR4-SNP n = 5; WT Day 11 n = 9; TLR4-SNP Day 11 n = 9. (C) M2a (Mrc1, PPAR gamma ) protein production measured in DC samples in WT vs TLR4-SNP mice, comparing control and Day 11 samples. Data representative of results from two independent experiments totaling control WT n = 5; control TLR4-SNP n = 5; WT Day 11 n = 9; TLR4-SNP Day 11 n = 9. (D) beta -HB concentration measured in the sera of control and DSS-treated, WT vs TLR4-SNP male mice at Days 9 and 11. Two-way ANOVA with Sidak multiple comparisons. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Data are derived from three independent experiments in which, together, control WT n = 12; control TLR4-SNP n = 13; WT Day 11 n = 10; TLR4-SNP Day 11 n = 10. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37768050), licensed under a CC-BY license. Not internally tested by R&D Systems.

In vivo experiment of TA gel in the mouse a the protocol of in vivo experiment; b in vivo fluorescence images of the TA gel at different implantation time points; c representative WB stripes of iNOS and CD206 expressions in the stroke mice; quantitative WB results of d iNOS and e CD206; ethological results of f balance beam and g rotarod tests Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37908012), licensed under a CC-BY license. Not internally tested by R&D Systems.

In vitro cell culture a schematic diagram of BV2 cell culture; b BV2 cell viabilities treated with different TAG dilutions before and after OGD; c cytotoxicity by LDH measurement before and after OGD; d IF images of CD16 and CD206 staining in BV2 cells after OGD; semiquantitative results of e CD16 and f CD206 staining; g representative WB strips of CD16, IL-1 beta , CD206 and TGF-beta expression in microglia before and after OGD; quantitative WB results of h CD16, i IL-1 beta , j CD206 and k TGF-beta ; l N2a cell viabilities cocultured with BV2 cells before and after OGD; m schematic diagram of N2a and BV2 cell coculture; n representative WB stripes of synaptophysin and PSD95 expression in N2a coculture with BV2 cells before and after OGD; quantitative WB results of o synaptophysin and p PSD95 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37908012), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of human umbilical cord mesenchymal stem cell‐derived exosomes on the microglia cell polarization. (A and B) Western blot analysis and quantification of iNOS and COX‐2. (C and D) Western blot analysis and quantification of YM1 and ARG1. (E) Representative images of microglia bearing a pro‐inflammatory (M1) phenotype in each group were examined using anti‐CD86 (green) and anti‐Iba‐1 (red) antibodies, as well as DAPI (blue); scale bar = 25 μm. (F) Representative images of anti‐inflammatory phenotype microglia in each group were examined using anti‐CD206 (green), and anti‐Iba‐1 (red) antibodies, as well as DAPI (blue); scale bar = 25 μm. (G) Relative fluorescence intensity analysis for CD86 and CD206. (H) Representative images of primary microglia bearing a pro‐inflammatory phenotype in each group were examined using anti‐CD86 (green), anti‐Iba‐1 (red) antibodies, and DAPI (blue); scale bar = 25 μm. (I) Representative images of anti‐inflammatory phenotype primary microglia in each group were examined by using anti‐CD206 (green) and anti‐Iba‐1 (red) antibodies, as well as DAPI (blue); scale bar = 25 μm. (J) Relative fluorescence intensity analysis for CD86 and CD206 in primary microglia; n = 3; mean ± SD; *p < 0.05; **p < 0.01; ***p < 0.001, compared with the control group; #p < 0.05; ##p < 0.01; ###p < 0.001, compared with the LPS group. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37697971), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Mu
• Applications: WB, Flow, IHC, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

MMR/CD206/Mannose Receptor Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse MMR/CD206
  Leu19-Ala1388
  Accession # Q2HZ94
• Specificity: Detects mouse MMR/CD206 in direct ELISAs and Western blots. In direct ELISAs, less than 45% cross-reactivity with recombinant human MMR is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MRC1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MMR/CD206/Mannose Receptor Antibody [Unconjugated]

• CD206
• CLEC13D
• CLEC13Dmacrophage mannose receptor 1
• C-type lectin domain family 13 member D
• mannose receptor, C type 1
• MMR
• MMRCD206 antigen
• MRC1

Background

The mouse Macrophage Mannose Receptor (MMR), also known as CD206 and MRC1 (mannose receptor C, type 1), is a 175 kDa scavenger receptor that is expressed on tissue macrophages, myeloid dendritic cells, and liver and lymphatic endothelial cells (1). It belongs to a family of receptors sharing similar protein structure that also includes DEC205, phospholipase A2 receptor, and Endo180 (2, 3). The mouse MMR protein is synthesized as a 1456 amino acid (aa) precursor that contains a 19 aa signal sequence, a 1369 aa extracellular region, a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (4). Its extracellular region is composed of an N-terminal cysteine-rich domain, followed by a single fibronectin type II repeat, and eight C-type lectin carbohydrate recognition domains (CRD) (3‑5). Mouse to human, the extracellular region is 82% aa identical. The cysteine-rich domain mediates recognition of sulfated N-acetylgalactosamine, which occurs on some extracellular matrix proteins and is the terminal sugar of the unusual oligosaccharides present on pituitary hormones such as lutropin and thyrotropin (6). Several of the CRDs participate in the Ca²⁺-dependent recognition of carbohydrates showing a preference for branched sugars with terminal mannose, fucose or N‑acetylglucosamine (7). The cytoplasmic domain of MMR includes a tyrosine-based motif for internalization in clathrin-coated vesicles. Once internalized, ligands are released following acidification of phagosomes or endosomes, and the receptor recycles to the cell surface (3, 8). MMR mediates phagocytosis upon binding to target structures that occur on a variety of pathogenic microorganisms including Gram-negative and Gram-positive bacteria, yeasts, parasites, and mycobacteria. MMR also functions to maintain homeostasis through the endocytosis of potentially harmful glycoproteins associated with inflammation (2, 3).

1. East, L. and C. Isake (2002) Biochim. Biophys. Acta 1572:364. 
2. Chieppa, M. et al. (2003) J. Immunol. 171:4552. 
3. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77. 
4. Harris, N. et al. (1992) Blood 80:2363.
5. Taylor, M. et al. (1990) J. Biol. Chem. 265:12156.
6. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
7. Martinez-Pomares, L. et al. (2001) Immunobiology 204:527.
8. Feinberg, H. et al. (2000) J. Biol. Chem. 275:21539.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MMR/CD206/Mannose Receptor Antibody (AF2535)(198)

Publications using AF2535

• Ali, H;Khan, F;Xuan, W;Liu, Y;Huang, Y;Whitfield, D;Pang, L;Chen, P; Neuropeptide adrenomedullin remodels stemness and macrophage dynamics in glioblastoma Cell reports 2025-09-24 [PMID: 41004340] (Immunohistochemistry, Western Blot, Mouse)
• Liang, X;Song, C;Lin, J;Li, S;Li, L;Dai, G;Zhang, R;Zou, OM;Yao, H;Zhou, L;Zou, Y; Transthyretin, a novel prognostic marker of POCD revealed by time-series RNA-sequencing analysis Molecular psychiatry 2025-02-15 [PMID: 39955470] (Immunohistochemistry, Western Blot, Human, Mouse)
• Pan, Y;Nie, L;Chen, W;Guan, D;Li, Y;Yang, C;Duan, L;Wan, T;Zhuang, L;Lai, J;Li, W;Zhang, Y;Wang, Q; Buyang Huanwu Decoction prevents hemorrhagic transformation after delayed t-PA infusion via inhibiting NLRP3 inflammasome/pyroptosis associated with microglial PGC-1? Journal of ethnopharmacology 2024-12-20 [PMID: 39710159] (Immunohistochemistry, Rat)
• Zhang, Y;Zhang, S;Yang, L;Zhang, Y;Cheng, Y;Jia, P;Lv, Y;Wang, K;Fan, P;Zhang, P;Wei, H; Lactate modulates microglial inflammatory responses through HIF-1?-mediated CCL7 signaling after cerebral ischemia in mice International immunopharmacology 2024-12-14 [PMID: 39675197] (Immunohistochemistry, Mouse)
• Kong, L;Xu, P;Shen, N;Li, W;Li, R;Tao, C;Wang, G;Zhang, Y;Sun, W;Hu, W;Liu, X; STING orchestrates microglia polarization via interaction with LC3 in autophagy after ischemia Cell death & disease 2024-11-13 [PMID: 39537618] (Immunohistochemistry, Western Blot, Mouse)
• Tanaka, T;Isonishi, A;Banja, M;Yamamoto, R;Sonobe, M;Okuda-Ashitaka, E;Furue, H;Okuda, H;Tatsumi, K;Wanaka, A; Dermal macrophages control tactile perception under physiological conditions via NGF signaling Scientific reports 2024-11-08 [PMID: 39516548] (Western Blot, Transgenic Mouse)
• Liao, R;Dewey, MJ;Rong, J;Johnson, SA;D'Angelo, WA;Hussey, GS;Badylak, SF; Matrix-bound nanovesicles alleviate particulate-induced periprosthetic osteolysis Science advances 2024-10-18 [PMID: 39423278] (Immunohistochemistry, Mouse)
• Korte, N;Barkaway, A;Wells, J;Freitas, F;Sethi, H;Andrews, SP;Skidmore, J;Stevens, B;Attwell, D; Inhibiting Ca2+ channels in Alzheimer's disease model mice relaxes pericytes, improves cerebral blood flow and reduces immune cell stalling and hypoxia Nature neuroscience 2024-09-18 [PMID: 39294491] (Immunohistochemistry, Mouse)
• Chen, D;Zhou, Z;Kong, N;Xu, T;Liang, J;Xu, P;Yao, B;Zhang, Y;Sun, Y;Li, Y;Wu, B;Yang, X;Wang, H; Inhalable SPRAY nanoparticles by modular peptide assemblies reverse alveolar inflammation in lethal Gram-negative bacteria infection Science advances 2024-09-13 [PMID: 39270015] (Immunohistochemistry, Immunocytochemistry, Mouse)
• Sutthiwanjampa, C;Kang, SH;Kim, MK;Hwa Choi, J;Kim, HK;Woo, SH;Bae, TH;Kim, WJ;Kang, SH;Park, H; Tumor necrosis factor-?-treated human adipose-derived stem cells enhance inherent radiation tolerance and alleviate in vivo radiation-induced capsular contracture Journal of advanced research 2024-07-15 [PMID: 39019109] (IHC-Pr, Human)
• Su, Q;Su, C;Zhang, Y;Guo, Y;Liu, Y;Liu, Y;Yong, VW;Xue, M; Adjudin protects blood-brain barrier integrity and attenuates neuroinflammation following intracerebral hemorrhage in mice International immunopharmacology 2024-04-01 [PMID: 38565042] (Immunohistochemistry, Mouse)
• Mader, MM;Napole, A;Wu, D;Atkins, M;Scavetti, A;Shibuya, Y;Foltz, A;Hahn, O;Yoo, Y;Danziger, R;Tan, C;Wyss-Coray, T;Steinman, L;Wernig, M; Myeloid cell replacement is neuroprotective in chronic experimental autoimmune encephalomyelitis Nature neuroscience 2024-03-21 [PMID: 38514857] (Immunohistochemistry, Mouse)
• Del Pilar, C;Garrido-Matilla, L;Del Pozo-Filíu, L;Lebrón-Galán, R;Arias, RF;Clemente, D;Alonso, JR;Weruaga, E;Díaz, D; Intracerebellar injection of monocytic immature myeloid cells prevents the adverse effects caused by stereotactic surgery in a model of cerebellar neurodegeneration Journal of neuroinflammation 2024-02-14 [PMID: 38355633] (IHC, Mouse)
• Surve, CR;Duran, CL;Ye, X;Chen, X;Lin, Y;Harney, AS;Wang, Y;Sharma, VP;Stanley, ER;McAuliffe, JC;Entenberg, D;Oktay, MH;Condeelis, JS; Signaling events at TMEM doorways provide potential targets for inhibiting breast cancer dissemination bioRxiv : the preprint server for biology 2024-01-09 [PMID: 38260319] (IHC, Mouse)
• Vlk, AM;Prantner, D;Shirey, KA;Perkins, DJ;Buzza, MS;Thumbigere-Math, V;Keegan, AD;Vogel, SN; M2a macrophages facilitate resolution of chemically-induced colitis in TLR4-SNP mice mBio 2023-09-28 [PMID: 37768050] (Western Blot, Mouse)
• Zhang, Y;Park, YS;Kim, IB; A Distinct Microglial Cell Population Expressing Both CD86 and CD206 Constitutes a Dominant Type and Executes Phagocytosis in Two Mouse Models of Retinal Degeneration International journal of molecular sciences 9/18/2023 [PMID: 37762541]
• Fiore, NT;Keating, BA;Chen, Y;Williams, SI;Moalem-Taylor, G; Differential Effects of Regulatory T Cells in the Meninges and Spinal Cord of Male and Female Mice with Neuropathic Pain Cells 2023-09-20 [PMID: 37759539] (IHC, Mouse)
• Ye, K;Zhang, X;Shangguan, L;Liu, X;Nie, X;Qiao, Y; Manganese-Implanted Titanium Modulates the Crosstalk between Bone Marrow Mesenchymal Stem Cells and Macrophages to Improve Osteogenesis Journal of functional biomaterials 2023-09-03 [PMID: 37754870] (ICC, Mouse)
• Walter, J;Mende, J;Hutagalung, S;Alhalabi, OT;Grutza, M;Zheng, G;Skutella, T;Unterberg, A;Zweckberger, K;Younsi, A; The Single-Dose Application of Interleukin-4 Ameliorates Secondary Brain Damage in the Early Phase after Moderate Experimental Traumatic Brain Injury in Mice International journal of molecular sciences 2023-08-14 [PMID: 37628939] (IHC, Mouse)
• Halawani, D;Wang, Y;Ramakrishnan, A;Estill, M;He, X;Shen, L;Friedel, RH;Zou, H; Circadian clock regulator Bmal1 gates axon regeneration via Tet3 epigenetics in mouse sensory neurons Nature communications 2023-08-24 [PMID: 37620297] (Immunohistochemistry, Transgenic Mouse)
• Honorine Dushime, Stéphanie G. Moreno, Christine Linard, Annie Adrait, Yohann Couté, Juliette Peltzer et al. Fetal Muse-based therapy prevents lethal radio-induced gastrointestinal syndrome by intestinal regeneration Stem Cell Research & Therapy 2023-08-11 [PMID: 37568164]
• Hong Y, Wang Y, Cui Y et al. MicroRNA-124-3p Attenuated Retinal Neovascularization in Oxygen-Induced Retinopathy Mice by Inhibiting the Dysfunction of Retinal Neuroglial Cells through STAT3 Pathway International journal of molecular sciences 2023-07-21 [PMID: 37511525]
• Chen S, Xing Z, Geng M et al. Macrophage fusion event as one prerequisite for inorganic nanoparticle-induced antitumor response Science advances 2023-07-21 [PMID: 37467339]
• Chen M, Menon MC, Wang W et al. HCK induces macrophage activation to promote renal inflammation and fibrosis via suppression of autophagy Nature communications 2023-07-18 [PMID: 37463911]
• Wu, L;Yan, J;Bai, Y;Chen, F;Zou, X;Xu, J;Huang, A;Hou, L;Zhong, Y;Jing, Z;Yu, Q;Zhou, X;Jiang, Z;Wang, C;Cheng, M;Ji, Y;Hou, Y;Luo, R;Li, Q;Wu, L;Cheng, J;Wang, P;Guo, D;Huang, W;Lei, J;Liu, S;Yan, Y;Chen, Y;Liao, S;Li, Y;Sun, H;Yao, N;Zhang, X;Zhang, S;Chen, X;Yu, Y;Li, Y;Liu, F;Wang, Z;Zhou, S;Yang, H;Yang, S;Xu, X;Liu, L;Gao, Q;Tang, Z;Wang, X;Wang, J;Fan, J;Liu, S;Yang, X;Chen, A;Zhou, J; An invasive zone in human liver cancer identified by Stereo-seq promotes hepatocyte-tumor cell crosstalk, local immunosuppression and tumor progression Cell research 2023-06-19 [PMID: 37337030] (IHC, Human)
• Pérez-Martín, E;Pérez-Revuelta, L;Barahona-López, C;Pérez-Boyero, D;Alonso, JR;Díaz, D;Weruaga, E; Oleoylethanolamide Treatment Modulates Both Neuroinflammation and Microgliosis, and Prevents Massive Leukocyte Infiltration to the Cerebellum in a Mouse Model of Neuronal Degeneration International journal of molecular sciences 2023-06-02 [PMID: 37298639] (IHC, Mouse)
• Lee S, Baek S, Lee Y et al. Ginsenoside Rg3-enriched Korean red ginseng extract attenuates Non-Alcoholic Fatty Liver Disease by way of suppressed VCAM-1 expression in liver sinusoidal endothelium Journal of Ginseng Research 2022-10-01 [PMID: 37252282] (Immunohistochemistry, Mouse)
• Can Wang, Qin Zhao, Chen Chen, Jiaojiao Li, Jing Zhang, Shuyuan Qu et al. CD301b+ macrophage: the new booster for activating bone regeneration in periodontitis treatment International Journal of Oral Science 2023-05-17 [PMID: 37198150] (Immunohistochemistry, Mouse)
• Choconta JL, Labi V, Dumbraveanu C et al. Age-related neuroimmune signatures in dorsal root ganglia of a Fabry disease mouse model Immunity & ageing : I & A 2023-05-12 [PMID: 37173694]
  
  Details:
  IHC 1:100, Antigen rerieval: sodium citrate 80C/30min
• HE Jones, V Coelho-San, SK Bonney, KA Abrams, AY Shih, JA Siegenthal Meningeal origins and dynamics of perivascular fibroblast development on the mouse cerebral vasculature bioRxiv : the preprint server for biology, 2023-03-23;0(0):. 2023-03-23 [PMID: 36993587] (IHC, Mouse)
• MM Mader, A Napole, D Wu, Y Shibuya, A Scavetti, A Foltz, M Atkins, O Hahn, Y Yoo, R Danziger, C Tan, T Wyss-Coray, L Steinman, M Wernig Augmentation of a neuroprotective myeloid state by hematopoietic cell transplantation bioRxiv : the preprint server for biology, 2023-03-12;0(0):. 3/12/2023 [PMID: 36945385]
• Xinyu Niu, Yuguo Xia, Lei Luo, Yu Chen, Ji Yuan, Juntao Zhang et al. iPSC-sEVs alleviate microglia senescence to protect against ischemic stroke in aged mice Materials Today Bio 2023-04-01 [PMID: 36936398]
• JX Wang, B Cao, N Ma, KY Wu, WB Chen, W Wu, X Dong, CF Liu, YF Gao, TY Diao, XY Min, Q Yong, ZF Li, W Zhou, K Li Collectin-11 promotes cancer cell proliferation and tumor growth JCI Insight, 2023-03-08;8(5):. 3/8/2023 [PMID: 36883567]
• F Jiang, X Qi, X Wu, S Lin, J Shi, W Zhang, X Jiang Regulating macrophage-MSC interaction to optimize BMP-2-induced osteogenesis in the local microenvironment Bioactive materials, 2023-02-11;25(0):307-318. 2023-02-11 [PMID: 36844362] (ICC, Mouse)
• L Pang, M Dunterman, W Xuan, A Gonzalez, Y Lin, WH Hsu, F Khan, RS Hagan, WA Muller, AB Heimberger, P Chen Circadian regulator CLOCK promotes tumor angiogenesis in glioblastoma Cell Reports, 2023-02-14;42(2):112127. 2023-02-14 [PMID: 36795563] (IHC, Mouse)
• Li Y, Kubo H, Yu D et al. Combining three independent pathological stressors induces a heart failure with preserved ejection fraction phenotype American journal of physiology. Heart and circulatory physiology 2023-04-01 [PMID: 36763506]
  
  Details:
  1:100 dilution
• Y Hattori, D Kato, F Murayama, S Koike, H Asai, A Yamasaki, Y Naito, A Kawaguchi, H Konishi, M Prinz, T Masuda, H Wake, T Miyata CD206+ macrophages transventricularly infiltrate the early embryonic cerebral wall to differentiate into microglia Cell Reports, 2023-02-07;42(2):112092. 2023-02-07 [PMID: 36753421] (IHC, Mouse)
• Bo-Yoon Park, Dirong Wu, Kyoo-Ri Kwon, Mi-Jin Kim, Tae-Gon Kim, Jun-Ho Lee et al. Implantation and tracing of green fluorescent protein-expressing adipose-derived stem cells in peri-implant capsular fibrosis Stem Cell Research & Therapy 2023-02-08 [PMID: 36750973]
• Siqi Sheng, Yixian Ma, Yue Zou, Fangyuan Hu, Ling Chen Protective effects of blocking PD-1 pathway on retinal ganglion cells in a mouse model of chronic ocular hypertension Frontiers in Immunology 2023-01-18 [PMID: 36741384]
• Q Zhu, X Zhou, Y Zhang, D Ye, K Yu, W Cao, L Zhang, H Zheng, Z Sun, C Guo, X Hong, Y Zhu, Y Zhang, Y Xiao, TG Valencak, T Ren, D Ren White-light crosslinkable milk protein bioadhesive with ultrafast gelation for first-aid wound treatment Biomaterials research, 2023-02-03;27(1):6. 2023-02-03 [PMID: 36737833] (IHC, Mouse)
• T Tanaka, H Okuda, A Isonishi, Y Terada, M Kitabatake, T Shinjo, K Nishimura, S Takemura, H Furue, T Ito, K Tatsumi, A Wanaka Dermal macrophages set pain sensitivity by modulating the amount of tissue NGF through an SNX25-Nrf2 pathway Nature Immunology, 2023-01-26;0(0):. 2023-01-26 [PMID: 36703006] (Western Blot, Mouse)
• Lorena Olivares-González, Sheyla Velasco, Idoia Gallego, Marina Esteban-Medina, Gustavo Puras, Carlos Loucera et al. An SPM-Enriched Marine Oil Supplement Shifted Microglia Polarization toward M2, Ameliorating Retinal Degeneration in rd10 Mice Antioxidants (Basel) 2022-12-30 [PMID: 36670960]
• Tae-Ryong Riew, Ji-Won Hwang, Xuyan Jin, Hong Lim Kim, Mun-Yong Lee Infiltration of meningeal macrophages into the Virchow–Robin space after ischemic stroke in rats: Correlation with activated PDGFR-beta -positive adventitial fibroblasts Frontiers in Molecular Neuroscience 2022-12-30 [PMID: 36644619]
• N Cousin, S Bartel, J Scholl, C Tacconi, A Egger, G Thorhallsd, D Neri, LC Dieterich, M Detmar Antibody-Mediated Delivery of VEGF-C Promotes Long-Lasting Lymphatic Expansion That Reduces Recurrent Inflammation Cells, 2022-12-31;12(1):. 2022-12-31 [PMID: 36611965] (IHC, Mouse)
• Jingman Li, Yuchen Pan, Jingjing Yang, Jiali Wang, Qi Jiang, Huan Dou et al. Tumor necrosis factor-alpha -primed mesenchymal stem cell-derived exosomes promote M2 macrophage polarization via Galectin-1 and modify intrauterine adhesion on a novel murine model Frontiers in Immunology 2022-12-16 [PMID: 36591221]
• Heng-Li Tian, Zhi-Ming Zhang, Shi-Wen Ding, Yao Jing, Shi-Ming Zhang, Shi-Wen Chen et al. Inhibiting phosphatase and actin regulator 1 expression is neuroprotective in the context of traumatic brain injury Neural Regeneration Research 2023-01-01 [PMID: 36571365]
• J Li, S Sudiwala, L Berthoin, S Mohabbat, EA Gaylord, H Sinada, N Cruz Pache, JC Chang, O Jeon, IMA Lombaert, AJ May, E Alsberg, CS Bahney, SM Knox Long-term functional regeneration of radiation-damaged salivary glands through delivery of a neurogenic hydrogel Science Advances, 2022-12-21;8(51):eadc8753. 2022-12-21 [PMID: 36542703] (Immunohistochemistry, Mouse)
• Cappellesso F, Orban MP, Shirgaonkar N et al. Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancer Nature cancer 2022-12-01 [PMID: 36522548]
• S Li, H Liu, Y Qian, L Jiang, S Liu, Y Liu, C Liu, X Gu IL-33/ST2 axis promotes remodeling of the extracellular matrix and drives protective microglial responses in the mouse model of perioperative neurocognitive disorders International immunopharmacology, 2022-11-26;114(0):109479. 2022-11-26 [PMID: 36446234] (Western Blot, Mouse)
• S Gaige, R Barbouche, M Barbot, S Boularand, M Dallaporta, A Abysique, JD Troadec Constitutively active microglial populations limit anorexia induced by the food contaminant deoxynivalenol Journal of Neuroinflammation, 2022-11-19;19(1):280. 2022-11-19 [PMID: 36403004] (IHC, Mouse)
• NM Sodir, L Pellegrine, RM Kortlever, T Campos, YW Kwon, S Kim, D Garcia, A Perfetto, P Anastasiou, LB Swigart, MJ Arends, TD Littlewood, GI Evan Reversible Myc hypomorphism identifies a key Myc-dependency in early cancer evolution Nature Communications, 2022-11-09;13(1):6782. 2022-11-09 [PMID: 36351945] (IHC, Mouse)
• Liqiang Liu, Jia Liu, Ming Li, Junxuan Lyu, Wei Su, Shejun Feng et al. Selective brain hypothermia attenuates focal cerebral ischemic injury and improves long‐term neurological outcome in aged female mice CNS Neuroscience & Therapeutics 2023-01-01 [PMID: 36341958]
• Mai Mohamed Bedeir, Yuzuru Ninoyu, Takashi Nakamura, Takahiro Tsujikawa, Shigeru Hirano Multiplex immunohistochemistry reveals cochlear macrophage heterogeneity and local auditory nerve inflammation in cisplatin-induced hearing loss Frontiers in Neurology 2022-10-20 [PMID: 36341090]
• Aleksandra Boikova, Megan J. Bywater, Gregory A. Quaife-Ryan, Jasmin Straube, Lucy Thompson, Camilla Ascanelli et al. HRas and Myc synergistically induce cell cycle progression and apoptosis of murine cardiomyocytes Frontiers in Cardiovascular Medicine 2022-10-20 [PMID: 36337898]
• A Weigert, X Zheng, A Nenzel, K Turkowski, S Günther, E Strack, E Sirait-Fis, E Elwakeel, IM Kur, VS Nikam, C Valasaraja, H Winter, A Wissgott, R Voswinkel, F Grimminger, B Brüne, W Seeger, SS Pullamsett, R Savai Fibrocytes boost tumor-supportive phenotypic switches in the lung cancer niche via the endothelin system Nature Communications, 2022-10-14;13(1):6078. 2022-10-14 [PMID: 36241617] (IHC, Mouse)
• Q Wang, B Han, X Man, H Gu, J Sun Chuanzhitongluo regulates microglia polarization and inflammatory response in acute ischemic stroke Brain research bulletin, 2022-09-21;190(0):97-104. 2022-09-21 [PMID: 36152772] (IHC, Mouse)
• Agnieszka Chryplewicz, Julie Scotton, Mélanie Tichet, Anoek Zomer, Ksenya Shchors, Johanna A. Joyce et al. Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunity Cancer Cell 2022-10-10 [PMID: 36113478]
• T Ye, Z Chen, J Zhang, L Luo, R Gao, L Gong, Y Du, Z Xie, B Zhao, Q Li, Y Wang Large extracellular vesicles secreted by human iPSC-derived MSCs ameliorate tendinopathy via regulating macrophage heterogeneity Oncogene, 2022-08-26;21(0):194-208. 2022-08-26 [PMID: 36101856] (ICC, Mouse)
• Y Xie, X Chen, Y Li, S Chen, S Liu, Z Yu, W Wang Transforming growth factor-beta1 protects against LPC-induced cognitive deficit by attenuating pyroptosis of microglia via NF-kappaB/ERK1/2 pathways Journal of Neuroinflammation, 2022-07-28;19(1):194. 2022-07-28 [PMID: 35902863] (Western Blot, Mouse)
• MS Lee, J Jeon, S Park, J Lim, HS Yang Rationally designed bioactive milk-derived protein scaffolds enhanced new bone formation Oncogene, 2022-06-16;20(0):368-380. 2022-06-16 [PMID: 35784638] (IHC, Mouse)
• D Chen, C Peng, XM Ding, Y Wu, CJ Zeng, L Xu, WY Guo Interleukin-4 promotes microglial polarization toward a neuroprotective phenotype after retinal ischemia/reperfusion injury Oncogene, 2022-12-01;17(12):2755-2760. 2022-12-01 [PMID: 35662225] (IHC, Mouse)
• Zeng X, Chen B, Wang L et al. Chitosan@Puerarin hydrogel for accelerated wound healing in diabetic subjects by miR-29ab1 mediated inflammatory axis suppression Bioactive materials 1/1/2023 [PMID: 35600974]
• D Chen, J Li, Y Huang, P Wei, W Miao, Y Yang, Y Gao Interleukin 13 promotes long-term recovery after ischemic stroke by inhibiting the activation of STAT3 Journal of Neuroinflammation, 2022-05-16;19(1):112. 2022-05-16 [PMID: 35578342] (IHC, Mouse)
• C Zuo, H Cao, F Feng, G Li, Y Huang, L Zhu, Z Gu, Y Yang, J Chen, Y Jiang, F Wang Repetitive transcranial magnetic stimulation exerts anti-inflammatory effects via modulating glial activation in mice with chronic unpredictable mild stress-induced depression International immunopharmacology, 2022-04-30;109(0):108788. 2022-04-30 [PMID: 35504201] (IF, Western Blot, Mouse)
• V Trappetti, J Fazzari, C Fernandez-, L Smyth, M Potez, N Shintani, B de Breuyn, OA Martin, V Djonov Targeted Accumulation of Macrophages Induced by Microbeam Irradiation in a Tissue-Dependent Manner Biomedicines, 2022-03-22;10(4):. 2022-03-22 [PMID: 35453485] (IHC, Mouse)
• T Masuda, L Amann, G Monaco, R Sankowski, O Staszewski, M Krueger, F Del Gaudio, L He, N Paterson, E Nent, F Fernández-, A Yamasaki, M Frosch, M Fliegauf, LFP Bosch, H Ulupinar, N Hagemeyer, D Schreiner, C Dorrier, M Tsuda, C Grothe, A Joutel, R Daneman, C Betsholtz, U Lendahl, KP Knobeloch, T Lämmermann, J Priller, K Kierdorf, M Prinz Specification of CNS macrophage subsets occurs postnatally in defined niches Nature, 2022-04-20;604(7907):740-748. 2022-04-20 [PMID: 35444273] (IHC, Mouse, Transgenic Mouse)
• JR Cortes, I Filip, R Albero, JA Patiño-Gal, SA Quinn, WW Lin, AP Laurent, BB Shih, JA Brown, AJ Cooke, A Mackey, J Einson, S Zairis, A Rivas-Delg, MA Laginestra, S Pileri, E Campo, G Bhagat, AA Ferrando, R Rabadan, T Palomero Oncogenic Vav1-Myo1f induces therapeutically targetable macrophage-rich tumor microenvironment in peripheral T�cell lymphoma Cell Reports, 2022-04-19;39(3):110695. 2022-04-19 [PMID: 35443168] (Flow Cytometry, IHC, Mouse)
• Y Wang, Q Sun, Y Ye, X Sun, S Xie, Y Zhan, J Song, X Fan, B Zhang, M Yang, L Lv, K Hosaka, Y Yang, G Nie FGF-2 signaling in nasopharyngeal carcinoma modulates pericyte-macrophage crosstalk and metastasis JCI Insight, 2022-05-23;0(0):. 2022-05-23 [PMID: 35439170] (IHC, Mouse)
• Atsuto Onoda, Shin Hagiwara, Natsuko Kubota, Shinya Yanagita, Ken Takeda, Masakazu Umezawa A Novel Staining Method for Detection of Brain Perivascular Injuries Induced by Nanoparticle: Periodic Acid-Schiff and Immunohistochemical Double-Staining Frontiers in Toxicology 2022-03-21 [PMID: 35391824]
• Li-Hua Cao, Yuan-Yuan Zhao, Ming Bai, David Geliebter, Jan Geliebter, Raj Tiwari et al. Mechanistic Studies of Gypenosides in Microglial State Transition and its Implications in Depression-Like Behaviors: Role of TLR4/MyD88/NF-kappa B Signaling Frontiers in Pharmacology 3/15/2022 [PMID: 35370734]
• Jesus Maria Gómez-Salinero, Franco Izzo, Yang Lin, Sean Houghton, Tomer Itkin, Fuqiang Geng et al. Specification of fetal liver endothelial progenitors to functional zonated adult sinusoids requires c-Maf induction Cell Stem Cell 2022-04-01 [PMID: 35364013]
• Y Geng, J Yang, X Cheng, Y Han, F Yan, C Wang, X Jiang, X Meng, M Fan, M Zhao, L Zhu A bioactive gypenoside (GP-14) alleviates neuroinflammation and blood brain barrier (BBB) disruption by inhibiting the NF-kappaB signaling pathway in a mouse high-altitude cerebral edema (HACE) model International immunopharmacology, 2022-03-14;107(0):108675. 2022-03-14 [PMID: 35299003] (IHC, Mouse)
• Bailey D. Peck, Kevin A. Murach, R. Grace Walton, Alexander J. Simmons, Douglas E. Long, Kate Kosmac et al. A muscle cell‐macrophage axis involving matrix metalloproteinase 14 facilitates extracellular matrix remodeling with mechanical loading The FASEB Journal 2022-02-01 [PMID: 35044708] (Immunohistochemistry, Human)
• T Zhou, C He, P Lai, Z Yang, Y Liu, H Xu, X Lin, B Ni, R Ju, W Yi, L Liang, D Pei, CE Egwuagu, X Liu miR-204-containing exosomes ameliorate GVHD-associated dry eye disease Science Advances, 2022-01-12;8(2):eabj9617. 2022-01-12 [PMID: 35020440] (IHC, Mouse)
• J Zhang, J Ma, RT Trinh, CJ Heijnen, A Kavelaars An HDAC6 inhibitor reverses chemotherapy-induced mechanical hypersensitivity via an IL-10 and macrophage dependent pathway Brain, Behavior, and Immunity, 2021-12-13;100(0):287-296. 2021-12-13 [PMID: 34915156] (IHC, Mouse)
• Kristina Alikhanyan, Yuanyuan Chen, Kalman Somogyi, Simone Kraut, Rocio Sotillo Mad2 Induced Aneuploidy Contributes to Eml4-Alk Driven Lung Cancer by Generating an Immunosuppressive Environment Cancers (Basel) 2021-11-30 [PMID: 34885137]
• Marcia G. Honig, Nobel A. Del Mar, Desmond L. Henderson, Dylan O’Neal, John B. Doty, Rachel Cox et al. Raloxifene Modulates Microglia and Rescues Visual Deficits and Pathology After Impact Traumatic Brain Injury Frontiers in Neuroscience 2021-10-29 [PMID: 34776838]
• M Seitz, C Köster, M Dzietko, H Sabir, M Serdar, U Felderhoff, I Bendix, J Herz Hypothermia modulates myeloid cell polarization in neonatal hypoxic-ischemic brain injury Journal of Neuroinflammation, 2021-11-13;18(1):266. 2021-11-13 [PMID: 34772426] (IHC, Mouse)
• HH Yu, XT Ma, X Ma, M Chen, YH Chu, LJ Wu, W Wang, C Qin, DS Tian Remote Limb Ischemic Postconditioning Protects Against Ischemic Stroke by Promoting Regulatory T Cells Thriving Journal of the American Heart Association, 2021-11-02;10(22):e023077. 2021-11-02 [PMID: 34726065] (ICC, IHC, Mouse)
• Jin L, Xiao L, Ding M Et al. Heterogeneous macrophages contribute to the pathology of disc herniation induced radiculopathy The spine journal : official journal of the North American Spine Society 10/27/2021 [PMID: 34718176]
• H Iwai, K Ataka, H Suzuki, A Dhar, E Kuramoto, A Yamanaka, T Goto Tissue-resident M2 macrophages directly contact primary sensory neurons in the sensory ganglia after nerve injury Journal of Neuroinflammation, 2021-10-13;18(1):227. 2021-10-13 [PMID: 34645458] (IF, Mouse)
• María López Chiloeches, Anna Bergonzini, Teresa Frisan, Océane C.B. Martin Characterization of macrophage infiltration and polarization by double fluorescence immunostaining in mouse colonic mucosa STAR Protocols 2021-12-01 [PMID: 34585165]
• Ravikanth Maddipati, Robert J. Norgard, Timour Baslan, Komal S. Rathi, Amy Zhang, Asal Saeid et al. MYC Levels Regulate Metastatic Heterogeneity in Pancreatic Adenocarcinoma Cancer Discovery 2022-02-01 [PMID: 34551968] (Immunohistochemistry, Transgenic Mouse)
• X Ma, C Qin, M Chen, HH Yu, YH Chu, TJ Chen, DB Bosco, LJ Wu, BT Bu, W Wang, DS Tian Regulatory T cells protect against brain damage by alleviating inflammatory response in neuromyelitis optica spectrum disorder Journal of Neuroinflammation, 2021-09-15;18(1):201. 2021-09-15 [PMID: 34526069] (IHC, Western Blot, Mouse)
• Lijuan Sun, Tim Kees, Ana Santos Almeida, Bodu Liu, Xue-Yan He, David Ng et al. Activating a collaborative innate-adaptive immune response to control metastasis Cancer Cell 2021-10-01 [PMID: 34478639]
• María José González-Riquelme, Caridad Galindo-Romero, Fernando Lucas-Ruiz, Marina Martínez-Carmona, Kristy T. Rodríguez-Ramírez, José María Cabrera-Maqueda et al. Axonal Injuries Cast Long Shadows: Long Term Glial Activation in Injured and Contralateral Retinas after Unilateral Axotomy International Journal of Molecular Sciences 2021-08-07 [PMID: 34445225]
• Akshay Gurdita, Philip E.B. Nickerson, Neno T. Pokrajac, Arturo Ortín-Martínez, En Leh Samuel Tsai, Lacrimioara Comanita et al. InVision: An optimized tissue clearing approach for three-dimensional imaging and analysis of intact rodent eyes iScience 2021-08-20 [PMID: 34430805]
• X Zhou, YN Zhang, FF Li, Z Zhang, LY Cui, HY He, X Yan, WB He, HS Sun, ZP Feng, SF Chu, NH Chen Neuronal chemokine-like-factor 1 (CKLF1) up-regulation promotes M1 polarization of microglia in rat brain after stroke Acta pharmacologica Sinica, 2021-08-12;0(0):. 2021-08-12 [PMID: 34385606] (IHC, Rat)
• M Fremder, SW Kim, A Khamaysi, L Shimshilas, H Eini-Rider, IS Park, U Hadad, JH Cheon, E Ohana A transepithelial pathway delivers succinate to macrophages, thus perpetuating their pro-inflammatory metabolic state Cell Reports, 2021-08-10;36(6):109521. 2021-08-10 [PMID: 34380041] (Western Blot, Mouse)
• Sílvia Gras, Alba Blasco, Guillem Mòdol-Caballero, Olga Tarabal, Anna Casanovas, Lídia Piedrafita et al. Beneficial effects of dietary supplementation with green tea catechins and cocoa flavanols on aging-related regressive changes in the mouse neuromuscular system Aging (Albany NY) 2021-07-31 [PMID: 34319911]
• L Zhang, J Chen, L Yan, Q He, H Xie, M Chen Resveratrol Ameliorates Cardiac Remodeling in a Murine Model of Heart Failure With Preserved Ejection Fraction Frontiers in Pharmacology, 2021-06-10;12(0):646240. 2021-06-10 [PMID: 34177571] (IHC, Mouse)
• Juehong Li, Ziyang Sun, Gang Luo, Shuo Wang, Haomin Cui, Zhixiao Yao et al. Quercetin Attenuates Trauma-Induced Heterotopic Ossification by Tuning Immune Cell Infiltration and Related Inflammatory Insult Frontiers in Immunology 2021-05-20 [PMID: 34093537] (Immunocytochemistry, Mouse)
• X Xu, W Gao, L Li, J Hao, B Yang, T Wang, L Li, X Bai, F Li, H Ren, M Zhang, L Zhang, J Wang, D Wang, J Zhang, L Jiao Annexin A1 protects against cerebral ischemia-reperfusion injury by modulating microglia/macrophage polarization via FPR2/ALX-dependent AMPK-mTOR pathway Journal of Neuroinflammation, 2021-05-22;18(1):119. 2021-05-22 [PMID: 34022892] (ICC, IHC, Mouse)
• Zhibing Song, Jingjing Feng, Qian Zhang, Shanshan Deng, Dahai Yu, Yuefan Zhang et al. Tanshinone IIA Protects Against Cerebral Ischemia Reperfusion Injury by Regulating Microglial Activation and Polarization via NF-kappa B Pathway Frontiers in Pharmacology 2021-04-19 [PMID: 33953677] (Immunohistochemistry, Rat)
• Z Germana, G Simona, L Marialucia, M Biagina, V Christine, F Paola, C Matteo, C Pasquale, M Davide, T Mario, M Massimilia, G Carlo, S Gaia, M Fabio Hypoxia-induced miR-210 modulates the inflammatory response and fibrosis upon acute ischemia Cell Death & Disease, 2021-05-01;12(5):435. 2021-05-01 [PMID: 33934122] (IHC, Mouse)
• F Garello, M Boido, M Miglietti, V Bitonto, M Zenzola, M Filippi, F Arena, L Consolino, M Ghibaudi, E Terreno Imaging of Inflammation in Spinal Cord Injury: Novel Insights on the Usage of PFC-Based Contrast Agents Biomedicines, 2021-04-03;9(4):. 2021-04-03 [PMID: 33916774] (ICC, IHC, Mouse)
• M Hu, Y Lin, X Men, S Wang, X Sun, Q Zhu, D Lu, S Liu, B Zhang, W Cai, Z Lu High-salt diet downregulates TREM2 expression and blunts efferocytosis of macrophages after acute ischemic stroke Journal of Neuroinflammation, 2021-04-12;18(1):90. 2021-04-12 [PMID: 33845849] (IHC, Mouse)
• A Chu, SY Kok, J Tsui, MC Lin, B Aguirre, M Wadehra Epithelial membrane protein 2 (Emp2) modulates innate immune cell population recruitment at the maternal-fetal interface Journal of reproductive immunology, 2021-03-09;145(0):103309. 2021-03-09 [PMID: 33774530] (IHC, Mouse)
• Niehaus JK, Taylor-Blake B, Loo L et al. Spinal macrophages resolve nociceptive hypersensitivity after peripheral injury Neuron 2/24/2021 [PMID: 33667343]
• Wahane S, Zhou X, Zhou X et al. Diversified transcriptional responses of myeloid and glial cells in spinal cord injury shaped by HDAC3 activity Science advances 2/1/2021 [PMID: 33637528]
• A Uneda, K Kurozumi, A Fujimura, K Fujii, J Ishida, Y Shimazu, Y Otani, Y Tomita, Y Hattori, Y Matsumoto, N Tsuboi, K Makino, S Hirano, A Kamiya, I Date Differentiated glioblastoma cells accelerate tumor progression by shaping the tumor microenvironment via CCN1-mediated macrophage infiltration Acta neuropathologica communications, 2021-02-22;9(1):29. 2021-02-22 [PMID: 33618763] (IHC, Mouse)
• Q Chang, Y Hao, Y Wang, Y Zhou, H Zhuo, G Zhao Bone marrow mesenchymal stem cell-derived exosomal microRNA-125a promotes M2 macrophage polarization in spinal cord injury by downregulating IRF5 Brain research bulletin, 2021-02-17;170(0):199-210. 2021-02-17 [PMID: 33609602] (Flow Cytometry, Mouse)
• Yunnan Lu, Mingming Zhou, Yun Li, Yan Li, Ye Hua, Yi Fan Minocycline promotes functional recovery in ischemic stroke by modulating microglia polarization through STAT1/STAT6 pathways Biochemical Pharmacology 2021-04-01 [PMID: 33577892]
• Mitri K. Khoury, Ting Zhou, Huan Yang, Samantha R. Prince, Kartik Gupta, Amelia R. Stranz et al. GSK2593074A blocks progression of existing abdominal aortic dilation JVS-Vascular Science 2021-02-11 [PMID: 33569546]
• Monisankar Ghosh, Suchandrima Saha, Julie Bettke, Rachana Nagar, Alejandro Parrales, Tomoo Iwakuma et al. Mutant p53 suppresses innate immune signaling to promote tumorigenesis Cancer Cell 2021-04-01 [PMID: 33545063] (Immunohistochemistry, Mouse)
• L Li, H Gan, H Jin, Y Fang, Y Yang, J Zhang, X Hu, L Chu Astragaloside IV promotes microglia/macrophages M2 polarization and enhances neurogenesis and angiogenesis through PPAR&amp;gamma pathway after cerebral ischemia/reperfusion injury in rats International immunopharmacology, 2021-01-08;92(0):107335. 2021-01-08 [PMID: 33429332] (IHC, Rat)
• Z Li, Y Song, T He, R Wen, Y Li, T Chen, S Huang, Y Wang, Y Tang, F Shen, HL Tian, GY Yang, Z Zhang M2 microglial small extracellular vesicles reduce glial scar formation via the miR-124/STAT3 pathway after ischemic stroke in mice Theranostics, 2021-01-01;11(3):1232-1248. 2021-01-01 [PMID: 33391532] (ICC, IHC, Mouse)
• David A. D. Munro, Barry M. Bradford, Samanta A. Mariani, David W. Hampton, Chris S. Vink, Siddharthan Chandran et al. CNS macrophages differentially rely on an intronic Csf1r enhancer for their development Development 2020-12-01 [PMID: 33323375]
• Xia Lei, Hanxia Li, Min Li, Qiwei Dong, Huayang Zhao, Zongyong Zhang et al. The novel Nrf2 activator CDDO‐EA attenuates cerebral ischemic injury by promoting microglia/macrophage polarization toward M2 phenotype in mice CNS Neuroscience & Therapeutics 2021-01-01 [PMID: 33280237] (Immunohistochemistry, Mouse)
• Y Nam, D Yoon, J Hong, MS Kim, TY Lee, KS Kim, HW Lee, K Suk, SR Kim Therapeutic Effects of Human Mesenchymal Stem Cells in a Mouse Model of Cerebellar Ataxia with Neuroinflammation J Clin Med, 2020-11-13;9(11):. 2020-11-13 [PMID: 33202913] (IHC, Western Blot, Mouse)
• M Chen, LL Yang, ZW Hu, C Qin, LQ Zhou, YL Duan, DB Bosco, LJ Wu, KB Zhan, SB Xu, DS Tian Deficiency of microglial Hv1 channel is associated with activation of autophagic pathway and ROS production in LPC-induced demyelination mouse model J Neuroinflammation, 2020-11-06;17(1):333. 2020-11-06 [PMID: 33158440] (IHC, Mouse)
• Amira Zawia, Nadine D. Arnold, Laura West, Josephine A. Pickworth, Helena Turton, James Iremonger et al. Altered Macrophage Polarization Induces Experimental Pulmonary Hypertension and Is Observed in Patients With Pulmonary Arterial Hypertension Arteriosclerosis, Thrombosis, and Vascular Biology 2021-01-01 [PMID: 33147993] (Immunohistochemistry, Mouse)
• Y Wang, Y Lin, L Wang, H Zhan, X Luo, Y Zeng, W Wu, X Zhang, F Wang TREM2 ameliorates neuroinflammatory response and cognitive impairment via PI3K/AKT/FoxO3a signaling pathway in Alzheimer&#039;s disease mice Aging (Albany NY), 2020-10-16;12(0):. 2020-10-16 [PMID: 33065553] (ICC, IHC, Mouse)
• Pires, A, Greenshields-Watson, A Et al. Immune Remodeling of the Extracellular Matrix Drives Loss of Cancer Stem Cells and Tumor Rejection. Cancer Immunol Res 12/1/2020 [PMID: 33023965]
• Hiroyuki Konishi, Takayuki Okamoto, Yuichiro Hara, Okiru Komine, Hiromi Tamada, Mitsuyo Maeda et al. Astrocytic phagocytosis is a compensatory mechanism for microglial dysfunction The EMBO Journal 2020-11-16 [PMID: 32959911]
• A Menga, M Serra, S Todisco, C Riera-Domi, U Ammarah, M Ehling, EM Palmieri, MA Di Noia, R Gissi, M Favia, CL Pierri, PE Porporato, A Castegna, M Mazzone Glufosinate constrains synchronous and metachronous metastasis by promoting anti-tumor macrophages EMBO Mol Med, 2020-09-04;0(0):e11210. 2020-09-04 [PMID: 32885605] (IHC, Mouse)
• D Wang, F Liu, L Zhu, P Lin, F Han, X Wang, X Tan, L Lin, Y Xiong FGF21 alleviates neuroinflammation following ischemic stroke by modulating the temporal and spatial dynamics of microglia/macrophages J Neuroinflammation, 2020-08-31;17(1):257. 2020-08-31 [PMID: 32867781] (IF, IHC, Mouse)
• Z. Khashim, D. Daying, D.Y. Hong, J.A. Ringler, S. Herting, D. Jakaitis et al. The Distribution and Role of M1 and M2 Macrophages in Aneurysm Healing after Platinum Coil Embolization American Journal of Neuroradiology 2020-08-13 [PMID: 32816763]
• Alessia Longoni, I. Pennings, Marta Cuenca Lopera, M. H. P. van Rijen, Victor Peperzak, A. J. W. P. Rosenberg et al. Endochondral Bone Regeneration by Non-autologous Mesenchymal Stem Cells Frontiers in Bioengineering and Biotechnology 2020-07-09 [PMID: 32733861]
• Harvey W. Smith, Lei Yang, Chen Ling, Arlan Walsh, Victor D. Martinez, Jonathan Boucher et al. An ErbB2 splice variant lacking exon 16 drives lung carcinoma Proceedings of the National Academy of Sciences 2020-08-18 [PMID: 32727899]
• A Pu, MK Mishra, Y Dong, S Ghorbaniga, EL Stephenson, KS Rawji, C Silva, H Kitagawa, S Sawcer, VW Yong The glycosyltransferase EXTL2 promotes proteoglycan deposition and injurious neuroinflammation following demyelination J Neuroinflammation, 2020-07-23;17(1):220. 2020-07-23 [PMID: 32703234] (IHC, Mouse)
• Alba Blasco, Sílvia Gras, Guillem Mòdol‐Caballero, Olga Tarabal, Anna Casanovas, Lídia Piedrafita et al. Motoneuron deafferentation and gliosis occur in association with neuromuscular regressive changes during ageing in mice Journal of Cachexia, Sarcopenia and Muscle 2020-12-01 [PMID: 32691534]
• Jialei Yang, Shipo Wu, Lihua Hou, Danni Zhu, Shimin Yin, Guodong Yang et al. Therapeutic Effects of Simultaneous Delivery of Nerve Growth Factor mRNA and Protein via Exosomes on Cerebral Ischemia Molecular Therapy - Nucleic Acids 2020-09-04 [PMID: 32682291]
• A Onoda, T Kawasaki, K Tsukiyama, K Takeda, M Umezawa Carbon nanoparticles induce endoplasmic reticulum stress around blood vessels with accumulation of misfolded proteins in the developing brain of offspring Sci Rep, 2020-06-22;10(1):10028. 2020-06-22 [PMID: 32572058] (IF, Mouse)
• Monique Marylin Alves de Almeida, Francesca Pieropan, Larissa de Mattos Oliveira, Manoelito Coelho dos Santos Junior, Jorge Mauricio David, Juceni Pereira David et al. The flavonoid agathisflavone modulates the microglial neuroinflammatory response and enhances remyelination Pharmacological Research 2020-09-01 [PMID: 32534098]
• F Jiang, W Zhang, M Zhou, Z Zhou, M Shen, N Chen, X Jiang Human amniotic mesenchymal stromal cells promote bone regeneration via activating endogenous regeneration Theranostics, 2020-05-15;10(14):6216-6230. 2020-05-15 [PMID: 32483449] (ICC, Neutralization, Human)
• Wang J, Lee CJ, Deci MB et al. MiR-101a loaded extracellular nanovesicles as bioactive carriers for cardiac repair Nanomedicine 4/8/2020 [PMID: 32278100]
• Y Hattori, Y Naito, Y Tsugawa, S Nonaka, H Wake, T Nagasawa, A Kawaguchi, T Miyata Transient microglial absence assists postmigratory cortical neurons in proper differentiation Nat Commun, 2020-04-02;11(1):1631. 2020-04-02 [PMID: 32242005] (IHC, Mouse)
• Abhijit Basu, Nina Dvorina, William M. Baldwin, Barsanjit Mazumder High‐fat diet‐induced GAIT element‐mediated translational silencing of mRNAs encoding inflammatory proteins in macrophage protects against atherosclerosis The FASEB Journal 2020-05-01 [PMID: 32232901]
• Ge Gao, Congcong Li, Jie Zhu, Yi Wang, Yunlong Huang, Shu Zhao et al. Glutaminase 1 Regulates Neuroinflammation After Cerebral Ischemia Through Enhancing Microglial Activation and Pro-Inflammatory Exosome Release Frontiers in Immunology 2020-02-07 [PMID: 32117296]
• de Haan W, Oie CI, Benkheil M et al. Unraveling the transcriptional determinants of Liver Sinusoidal Endothelial Cell specialization Am. J. Physiol. Gastrointest. Liver Physiol. 3/2/2020 [PMID: 32116021]
• Won-Sik Yeo, Chiamara Anokwute, Philip Marcadis, Marcus Levitan, Mahmoud Ahmed, Yeun Bae et al. A Membrane-Bound Transcription Factor is Proteolytically Regulated by the AAA+ Protease FtsH in Staphylococcus aureus Journal of Bacteriology 2020-04-09 [PMID: 32094161]
• W Miao, Y Zhao, Y Huang, D Chen, C Luo, W Su, Y Gao IL-13 Ameliorates Neuroinflammation and Promotes Functional Recovery after Traumatic Brain Injury J. Immunol., 2020-02-07;0(0):. 2020-02-07 [PMID: 32034062] (IHC, Mouse)
• M Sorkin, AK Huber, C Hwang, WF Carson, R Menon, J Li, K Vasquez, C Pagani, N Patel, S Li, ND Visser, Y Niknafs, S Loder, M Scola, D Nycz, K Gallagher, LK McCauley, J Xu, AW James, S Agarwal, S Kunkel, Y Mishina, B Levi Regulation of heterotopic ossification by�monocytes in a mouse model of aberrant wound healing Nat Commun, 2020-02-05;11(1):722. 2/5/2020 [PMID: 32024825]
• Francesca Ravanetti, Luisa Ragionieri, Roberta Ciccimarra, Francesca Ruscitti, Daniela Pompilio, Ferdinando Gazza et al. Modeling pulmonary fibrosis through bleomycin delivered by osmotic minipump: a new histomorphometric method of evaluation American Journal of Physiology-Lung Cellular and Molecular Physiology 2020-02-01 [PMID: 31851533]
• D Song, X Zhang, J Chen, X Liu, J Xue, L Zhang, X Lan Wnt canonical pathway activator TWS119 drives microglial anti-inflammatory activation and facilitates neurological recovery following experimental stroke J Neuroinflammation, 2019-12-06;16(1):256. 2019-12-06 [PMID: 31810470] (IHC-Fr, Mouse)
• AM Hamilton, WY Cheung, A Gómez-Aris, A Sharma, S Nakamura, A Chaboureau, S Bhatt, R Rabani, M Kapoor, PJ Foster, S Viswanatha Iron nanoparticle-labeled murine mesenchymal stromal cells in an osteoarthritic model persists and suggests anti-inflammatory mechanism of action PLoS ONE, 2019-12-03;14(12):e0214107. 2019-12-03 [PMID: 31794570] (IHC-P, Mouse)
• SY Ye, JE Apple, X Ren, FL Tang, LL Yao, YG Wang, L Mei, YG Zhou, WC Xiong Microglial VPS35 deficiency regulates microglial polarization and decreases ischemic stroke-induced damage in the cortex J Neuroinflammation, 2019-11-26;16(1):235. 2019-11-26 [PMID: 31771656] (IHC, Mouse)
• Jinjin Peng, Jiacheng Li, Jing Huang, Pan Xu, Heming Huang, Yanjun Liu et al. p300/CBP inhibitor A-485 alleviates acute liver injury by regulating macrophage activation and polarization Theranostics 2019-10-22 [PMID: 31754401]
• Shibata-Germanos S, Goodman JR, Grieg A et al. Structural and functional conservation of non-lumenized lymphatic endothelial cells in the mammalian leptomeninges Acta Neuropathol. 11/6/2019 [PMID: 31696318]
• W Cai, X Dai, J Chen, J Zhao, M Xu, L Zhang, B Yang, W Zhang, M Rocha, T Nakao, J Kofler, Y Shi, RA Stetler, X Hu, J Chen STAT6/Arg1 promotes microglia/macrophage efferocytosis and inflammation resolution in stroke mice JCI Insight, 2019-10-17;4(20):. 2019-10-17 [PMID: 31619589] (IHC-Fr, Mouse)
• Yi Xie, Li Ba, Min Wang, Sai‐Yue Deng, Si‐Miao Chen, Li‐Fang Huang et al. Chronic sleep fragmentation shares similar pathogenesis with neurodegenerative diseases: Endosome‐autophagosome‐lysosome pathway dysfunction and microglia‐mediated neuroinflammation CNS Neuroscience & Therapeutics 2020-02-01 [PMID: 31549780]
• CY Cui, RK Driscoll, Y Piao, CW Chia, M Gorospe, L Ferrucci Skewed macrophage polarization in aging skeletal muscle Aging Cell, 2019-09-02;0(0):e13032. 2019-09-02 [PMID: 31478346] (IHC-Fr, Mouse)
• Pino Bordignon, Giulia Bottoni, Xiaoying Xu, Alma S. Popescu, Zinnia Truan, Emmanuella Guenova et al. Dualism of FGF and TGF-beta Signaling in Heterogeneous Cancer-Associated Fibroblast Activation with ETV1 as a Critical Determinant Cell Reports 2019-08-27 [PMID: 31461652]
• Chen S, Ge L, Wang H et al. Eluted 25-hydroxyvitamin D3 from radially aligned nanofiber scaffolds enhances cathelicidin production while reducing inflammatory response in human immune system-engrafted mice Acta Biomater 8/3/2019 [PMID: 31386930]
• Ge Gao, Shu Zhao, Xiaohuan Xia, Chunhong Li, Congcong Li, Chenhui Ji et al. Glutaminase C Regulates Microglial Activation and Pro-inflammatory Exosome Release: Relevance to the Pathogenesis of Alzheimer’s Disease Frontiers in Cellular Neuroscience 2019-06-28 [PMID: 31316350]
• Schober ME, Requena DF, Casper TC Et al. Docosahexaenoic acid decreased neuroinflammation in rat pups after controlled cortical impact Exp Neurol 6/28/2019 [PMID: 31247195]
• Chen P, Zhao D, Li J et al. Symbiotic Macrophage-Glioma Cell Interactions Reveal Synthetic Lethality in PTEN-Null Glioma Cancer Cell 6/10/2019 [PMID: 31185211]
• T Koizumi, K Taguchi, I Mizuta, H Toba, M Ohigashi, O Onishi, K Ikoma, S Miyata, T Nakata, M Tanaka, S Foulquier, HWM Steinbusch, T Mizuno Transiently proliferating perivascular microglia harbor M1 type and precede cerebrovascular changes in a chronic hypertension model J Neuroinflammation, 2019-04-10;16(1):79. 2019-04-10 [PMID: 30971251] (IHC, Rat)
• Natalie M. Guley, Nobel A. Del Mar, Tyler Ragsdale, Chunyan Li, Aaron M. Perry, Bob M. Moore et al. Amelioration of visual deficits and visual system pathology after mild TBI with the cannabinoid type-2 receptor inverse agonist SMM-189 Experimental Eye Research 2019-05-01 [PMID: 30922891]
• Han YH, Shin KO, Kim JY et al. A maresin 1/ROR alpha/12-lipoxygenase autoregulatory circuit prevents inflammation and progression of nonalcoholic steatohepatitis J. Clin. Invest. 3/11/2019 [PMID: 30855276]
• S Morita-Tak, K Nakahara, S Hasegawa-I, A Isonishi, K Tatsumi, H Okuda, T Tanaka, M Kitabatake, T Ito, A Wanaka Responses of perivascular macrophages to circulating lipopolysaccharides in the subfornical organ with special reference to endotoxin tolerance J Neuroinflammation, 2019-02-14;16(1):39. 2019-02-14 [PMID: 30764851] (IHC-Fr, Mouse)
• Imke Rudnik-Jansen, Nina Woike, Suzanne de Jong, Sabine Versteeg, Marja Kik, Pieter Emans et al. Applicability of a Modified Rat Model of Acute Arthritis for Long-Term Testing of Drug Delivery Systems Pharmaceutics 2019-02-07 [PMID: 30736430] (Immunohistochemistry, Rat)
• V Bellver-La, F Bretheau, B Mailhot, N Vallières, M Lessard, ME Janelle, N Vernoux, MÈ Tremblay, T Fuehrmann, MS Shoichet, S Lacroix Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury Nat Commun, 2019-01-31;10(1):518. 2019-01-31 [PMID: 30705270] (IHC, Mouse)
• C Qin, Q Liu, ZW Hu, LQ Zhou, K Shang, DB Bosco, LJ Wu, DS Tian, W Wang Microglial TLR4-dependent autophagy induces ischemic white matter damage via STAT1/6 pathway Theranostics, 2018-10-29;8(19):5434-5451. 2018-10-29 [PMID: 30555556] (ICC, IHC-Fr, Western Blot, Mouse)
• Z An, CB Knobbe-Tho, X Wan, QW Fan, G Reifenberg, WA Weiss EGFR cooperates with EGFRvIII to recruit macrophages in glioblastoma Cancer Res., 2018-11-06;0(0):. 11/6/2018 [PMID: 30401716]
• Z. Zhuang, S. Yoshizawa-Smith, A. Glowacki, K. Maltos, C. Pacheco, M. Shehabeldin et al. Induction of M2 Macrophages Prevents Bone Loss in Murine Periodontitis Models Journal of Dental Research 2019-02-01 [PMID: 30392438] (Immunohistochemistry, Mouse)
• J Ye, Y Huang, B Que, C Chang, W Liu, H Hu, L Liu, Y Shi, Y Wang, M Wang, T Zeng, W Zhen, Y Xu, L Shi, J Liu, H Jiang, D Ye, Y Lin, J Wan, Q Ji Interleukin-12p35 Knock Out Aggravates Doxorubicin-Induced Cardiac Injury and Dysfunction by Aggravating the Inflammatory Response, Oxidative Stress, Apoptosis and Autophagy in Mice EBioMedicine, 2018-09-15;35(0):29-39. 2018-09-15 [PMID: 30228093] (IHC, Mouse)
• P Ayata, A Badimon, HJ Strasburge, MK Duff, SE Montgomery, YE Loh, A Ebert, AA Pimenova, BR Ramirez, AT Chan, JM Sullivan, I Purushotha, JR Scarpa, AM Goate, M Busslinger, L Shen, B Losic, A Schaefer Epigenetic regulation of brain region-specific microglia clearance activity Nat. Neurosci., 2018-07-23;21(8):1049-1060. 7/23/2018 [PMID: 30038282]
• K Hashimoto, M Nakashima, A Hamano, M Gotoh, H Ikeshima-K, K Murakami-M, Y Miyamoto 2-carba cyclic phosphatidic acid suppresses inflammation via regulation of microglial polarisation in the stab-wounded mouse cerebral cortex Sci Rep, 2018-06-26;8(1):9715. 2018-06-26 [PMID: 29946114] (ICC, Mouse)
• JA Lindborg, JP Niemi, MA Howarth, KW Liu, CZ Moore, D Mahajan, RE Zigmond Molecular and cellular identification of the immune response in peripheral ganglia following nerve injury J Neuroinflammation, 2018-06-26;15(1):192. 2018-06-26 [PMID: 29945607] (IHC-Fr, Mouse)
• Tian Zhou, Zijing Huang, Xiaowei Zhu, Xiaowei Sun, Yan Liu, Bing Cheng et al. Alpha-1 Antitrypsin Attenuates M1 Microglia-Mediated Neuroinflammation in Retinal Degeneration Frontiers in Immunology 2018-05-30 [PMID: 29899745]
• G Liu, P Lu, L Chen, W Zhang, M Wang, D Li, X Zhang B-cell leukemia/lymphoma 10 promotes angiogenesis in an experimental corneal neovascularization model Eye (Lond), 2018-03-08;0(0):. 2018-03-08 [PMID: 29515217] (Flow Cytometry, Mouse)
• S Kageyama, K Nakamura, B Ke, RW Busuttil, JW Kupiec-Weg Serelaxin Induces Notch1 Signaling and Alleviates Hepatocellular Damage in Orthotopic Liver Transplantation Am. J. Transplant., 2018-03-23;0(0):. 2018-03-23 [PMID: 29464890] (Western Blot, Mouse)
• X Liu, S Wen, F Yan, K Liu, L Liu, L Wang, S Zhao, X Ji Salidroside provides neuroprotection by modulating microglial polarization after cerebral ischemia J Neuroinflammation, 2018-02-09;15(1):39. 2018-02-09 [PMID: 29426336] (ICC, Mouse)
• CH Nijboer, E Kooijman, CT van Veltho, E Van Tilbor, IA Tiebosch, N Eijkelkamp, RM Dijkhuizen, J Kesecioglu, C Heijnen Intranasal stem cell treatment as a novel therapy for subarachnoid hemorrhage Stem Cells Dev., 2018-01-08;0(0):. 2018-01-08 [PMID: 29310519] (IHC-P, Rat)
• Kortlever Roderik M, Sodir Nicole M, Wilson Catherine H et al. Myc CoopeRates with Ras by Programming Inflammation and Immune Suppression. Cell 1/1/2017 [PMID: 29195074]
• Salli Antila, Sinem Karaman, Harri Nurmi, Mikko Airavaara, Merja H. Voutilainen, Thomas Mathivet et al. Development and plasticity of meningeal lymphatic vessels Journal of Experimental Medicine 2017-12-04 [PMID: 29141865]
• A Ladwig, HL Walter, J Hucklenbro, A Willuweit, KJ Langen, GR Fink, MA Rueger, M Schroeter Osteopontin Augments M2 Microglia Response and Separates M1- and M2-Polarized Microglial Activation in Permanent Focal Cerebral Ischemia Mediators Inflamm., 2017-09-20;2017(0):7189421. 9/20/2017 [PMID: 29104378]
• Feng Zhang, Rujia Zhong, Song Li, Zhenfa Fu, Cheng Cheng, Huaibin Cai et al. Acute Hypoxia Induced an Imbalanced M1/M2 Activation of Microglia through NF-kappa B Signaling in Alzheimer’s Disease Mice and Wild-Type Littermates Frontiers in Aging Neuroscience 2017-08-25 [PMID: 28890695]
• Hiroyuki Konishi, Masaaki Kobayashi, Taikan Kunisawa, Kenta Imai, Akira Sayo, Bernard Malissen et al. Siglec-H is a microglia-specific marker that discriminates microglia from CNS-associated macrophages and CNS-infiltrating monocytes Glia 2017-12-01 [PMID: 28836308]
• T Kuboyama, S Wahane, Y Huang, X Zhou, JK Wong, A Koemeter-C, M Martini, RH Friedel, H Zou HDAC3 inhibition ameliorates spinal cord injury by immunomodulation Sci Rep, 2017-08-17;7(1):8641. 2017-08-17 [PMID: 28819194] (IHC, Mouse)
• Weiwei Gao, Fei Li, Ziwei Zhou, Xin Xu, Yingang Wu, Shuai Zhou et al. IL-2/Anti-IL-2 Complex Attenuates Inflammation and BBB Disruption in Mice Subjected to Traumatic Brain Injury Frontiers in Neurology 2017-06-30 [PMID: 28713327] (Immunohistochemistry, Mouse)
• Jennifer Simkin, Thomas R Gawriluk, John C Gensel, Ashley W Seifert Macrophages are necessary for epimorphic regeneration in African spiny mice eLife 2017-05-16 [PMID: 28508748]
• Z Varasteh, F Hyafil, N Anizan, D Diallo, R Aid-Launai, S Mohanta, Y Li, M Braeuer, K Steiger, J Vigne, Z Qin, SG Nekolla, JE Fabre, Y Döring, D Le Guludec, A Habenicht, DR Vera, M Schwaiger Targeting mannose receptor expression on macrophages in atherosclerotic plaques of apolipoprotein E-knockout mice using (111)In-tilmanocept EJNMMI Res, 2017-05-03;7(1):40. 2017-05-03 [PMID: 28470406] (IHC, Mouse)
• Atsuto Onoda, Takayasu Kawasaki, Koichi Tsukiyama, Ken Takeda, Masakazu Umezawa Perivascular Accumulation of beta -Sheet-Rich Proteins in Offspring Brain following Maternal Exposure to Carbon Black Nanoparticles Frontiers in Cellular Neuroscience 2017-03-31 [PMID: 28408868]
• Y Yang, H Liu, H Zhang, Q Ye, J Wang, B Yang, L Mao, W Zhu, RK Leak, B Xiao, B Lu, J Chen, X Hu ST2/IL-33-Dependent Microglial Response Limits Acute Ischemic Brain Injury J. Neurosci., 2017-04-07;0(0):. 2017-04-07 [PMID: 28389473] (IHC, Mouse)
• MB Orr, J Simkin, WM Bailey, NH Kadambi, AL McVicar, AK Veldhorst, J Gensel Compression Decreases Anatomical and Functional Recovery and Alters Inflammation after Contusive Spinal Cord Injury J. Neurotrauma, 2017-06-14;0(0):. 6/14/2017 [PMID: 28381129]
• SJ Sung, Y Ge, C Dai, H Wang, SM Fu, R Sharma, YS Hahn, J Yu, TH Le, MD Okusa, WK Bolton, JR Lawler Dependence of Glomerulonephritis Induction on Novel Intraglomerular Alternatively Activated Bone Marrow-Derived Macrophages and Mac-1 and PD-L1 in Lupus-Prone NZM2328 Mice J. Immunol, 2017-02-20;0(0):. 2017-02-20 [PMID: 28219886] (Flow Cytometry, IHC, Mouse)
• JC Gensel, TJ Kopper, B Zhang, MB Orr, WM Bailey Predictive screening of M1 and M2 macrophages reveals the immunomodulatory effectiveness of post spinal cord injury azithromycin treatment Sci Rep, 2017-01-06;7(0):40144. 2017-01-06 [PMID: 28057928] (IHC-Fr, Mouse)
• Temporal Characterization of Microglia/Macrophage Phenotypes in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury Front Cell Neurosci, 2016-12-15;10(0):286. 2016-12-15 [PMID: 28018179] (IHC-Fr, Mouse)
• Rescue therapy with Tanshinone IIA hinders transition of acute kidney injury to chronic kidney disease via targeting GSK3? Sci Rep, 2016-11-18;6(0):36698. 2016-11-18 [PMID: 27857162] (IHC-Fr, Mouse)
• Juan Carlos Pena-Philippides, Ernesto Caballero-Garrido, Tamar Lordkipanidze, Tamara Roitbak In vivo inhibition of miR-155 significantly alters post-stroke inflammatory response Journal of Neuroinflammation 2016-12-01 [PMID: 27829437]
• Epameinondas Gousopoulos, Steven T. Proulx, Samia B. Bachmann, Jeannette Scholl, Dimitris Dionyssiou, Efterpi Demiri et al. Regulatory T cell transfer ameliorates lymphedema and promotes lymphatic vessel function JCI Insight 2016-10-06 [PMID: 27734032]
• Somayyeh Hamzei Taj, Widuri Kho, Markus Aswendt, Franziska M. Collmann, Claudia Green, Joanna Adamczak et al. Dynamic Modulation of Microglia/Macrophage Polarization by miR-124 after Focal Cerebral Ischemia Journal of Neuroimmune Pharmacology 2016-12-01 [PMID: 27539642]
• miR-155 Deletion in Mice Overcomes Neuron-Intrinsic and Neuron-Extrinsic Barriers to Spinal Cord Repair J Neurosci, 2016-08-10;36(32):8516-32. 2016-08-10 [PMID: 27511021] (IHC-Fr, Mouse)
• Alexander Schulz, Robert Büttner, Christian Hagel, Stephan L. Baader, Lan Kluwe, Johannes Salamon et al. The importance of nerve microenvironment for schwannoma development Acta Neuropathologica 2016-08-01 [PMID: 27236462]
• Raffaella Moretti, Pierre-Louis Leger, Valérie C. Besson, Zsolt Csaba, Julien Pansiot, Lorena Di Criscio et al. Sildenafil, a cyclic GMP phosphodiesterase inhibitor, induces microglial modulation after focal ischemia in the neonatal mouse brain Journal of Neuroinflammation 2016-12-01 [PMID: 27126393] (Immunohistochemistry, Mouse)
• Mario Catarinella, Andrea Monestiroli, Giulia Escobar, Amleto Fiocchi, Ngoc Lan Tran, Roberto Aiolfi et al. IFN alpha gene/cell therapy curbs colorectal cancer colonization of the liver by acting on the hepatic microenvironment EMBO Molecular Medicine 2016-02-01 [PMID: 26769348] (Immunohistochemistry, Mouse)
• Lloyd D. Harvey, Yan Yin, Insiya Y. Attarwala, Gulnaz Begum, Julia Deng, Hong Q. Yan et al. Administration of DHA Reduces Endoplasmic Reticulum Stress-Associated Inflammation and Alters Microglial or Macrophage Activation in Traumatic Brain Injury ASN Neuro 2015-12-20 [PMID: 26685193]
• Munkyung Kim, Alessandro Piaia, Neeta Shenoy, David Kagan, Berangere Gapp, Benjamin Kueng et al. Progression of Alport Kidney Disease in Col4a3 Knock Out Mice Is Independent of Sex or Macrophage Depletion by Clodronate Treatment PLOS ONE 2015-11-10 [PMID: 26555339]
• Jeffrey S. Hakim, Melika Esmaeili Rad, Peter J. Grahn, Bingkun K. Chen, Andrew M. Knight, Ann M. Schmeichel et al. Positively Charged Oligo[Poly(Ethylene Glycol) Fumarate] Scaffold Implantation Results in a Permissive Lesion Environment after Spinal Cord Injury in Rat Tissue Engineering Part A 2015-07-01 [PMID: 25891264]
• Kan M, Lee J, Wilson J, Everhart A, Brown C, Hoofnagle A, Jansen M, Vitek M, Gunn M, Colton C Arginine deprivation and immune suppression in a mouse model of Alzheimer&#039;s disease. J Neurosci, 2015-04-15;35(15):5969-82. 2015-04-15 [PMID: 25878270] (IHC-Fr, Mouse)
• Wang J, Xia J, Zhang F, Shi Y, Wu Y, Pu H, Liou A, Leak R, Yu X, Chen L, Chen J Galectin-1-secreting neural stem cells elicit long-term neuroprotection against ischemic brain injury. Sci Rep, 2015-04-10;5(0):9621. 2015-04-10 [PMID: 25858671] (IHC, Mouse)
• Pepe , Giovanna, Calderazzi , Giorgia, De Maglie , Marcella, Villa , Alessand, Vegeto , Elisabet Heterogeneous induction of microglia M2a phenotype by central administration of interleukin-4. J Neuroinflammation, 2014-12-31;11(1):211. 2014-12-31 [PMID: 25551794] (Western Blot, Mouse)
• Ko S, Ladanyi A, Lengyel E, Naora H Expression of the homeobox gene HOXA9 in ovarian cancer induces peritoneal macrophages to acquire an M2 tumor-promoting phenotype. Am J Pathol, 2014-01-01;184(1):271-81. 2014-01-01 [PMID: 24332016] (Flow Cytometry, Mouse)
• MicroRNA-124 as a novel treatment for persistent hyperalgesia. J Neuroinflammation, 2012-06-25;9(0):143. 2012-06-25 [PMID: 22731384] (IHC, Mouse)
• Liu Q, Cheng LI, Yi L, Zhu N, Wood A, Changpriroa CM, Ward JM, Jackson SH p47phox deficiency induces macrophage dysfunction resulting in progressive crystalline macrophage pneumonia. Am. J. Pathol., 2008-12-18;174(1):153-63. 2008-12-18 [PMID: 19095958] (IHC-P, Mouse)

Reviews for MMR/CD206/Mannose Receptor Antibody (AF2535) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 2 species: Mouse, Mouse and Rat.

reviewed by: Verified Customer IHC-Fr Mouse 01/18/2023

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Mouse Spleen
• Species: Mouse
• Lot: WFT0322071

reviewed by: Imke Jansen IHC-P Mouse and Rat 03/16/2018

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Testis
• Species: Mouse and Rat
• Lot: WFT0118011

Comments

• Comments: Mouse and Rat Testis: 4% Neutral buffered Formalin fixated for 3 days and subsequently dehydrated in alcohol steps (2x 70%, 2x 96%, 2x 100%) xyleen and paraffin embedded.
  
  Primary antibodies:
  1/100 dilution&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;MMR/CD206 Goat polyclonal IgG &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;AF2535
  Lot#WFT0118011
  1/500 dilution&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Goat IgG isotype 10 ug/ml&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;02-6202
  Lot#TA262200
  both working concentration: 10 ug/mL
  
  Since rat species have not been tested for MMR/CD206 antibody (AF2535), we decided to take part in the Innovator’s Reward™ program.
  MMR/CD206 was already detected in perfusion fixed frozen sections of mouse testis with specific staining localized to spermatocytes in testis. Therefore, we included mouse testis (positive control) and rat testis tissue, that is formalin fixated and paraffin embedded.

reviewed by: Verified Customer IHC-Fr Mouse 01/07/2015

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: See PMID 23942366
• Species: Mouse

Bioinformatics

• Gene Symbol: MRC1
• Uniprot:
  • Q2HZ94()