TopBP1 Antibody

Images

 
Western Blot: TopBP1 Antibody [NB100-217] - Detection of mouse TopBP1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified ...read more
Western Blot: TopBP1 Antibody [NB100-217] - Sample: Whole cell lysate (50 ug for WB) from HeLa cells.
Western Blot: TopBP1 Antibody [NB100-217] - WB analysis of TOPBP1 in HeLa cell extract mitotic and interphase. Image courtesy of product review submitted by Jennifer Flynn.
Western Blot: TopBP1 Antibody [NB100-217] - Detection of Mouse. Whole cell lysate from TCMK-1 (15 and 50 ug) cells.
Biological Strategies: Western Blot: TopBP1 Antibody [NB100-217] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOPBP1 ...read more
Western Blot: TopBP1 Antibody [NB100-217] - Detection of human TopBP1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity ...read more
Immunoprecipitation: TopBP1 Antibody [NB100-217] - Detection of human TopBP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

TopBP1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1400 and the C-terminus (residue 1435) of human Topoisomerase (DNA) II Binding Protein 1 using the numbering given in entry NP_008958.1 (GeneID 11073).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TOPBP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunoprecipitation 0.5 to 2.5 ug/mg of lysate
Reviewed Applications
Read 1 Review rated 3
using
NB100-217 in the following applications:

Publications
Read Publications using
NB100-217 in the following applications:

  • IP
    1 publication
  • WB
    5 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TopBP1 Antibody

  • DNA topoisomerase II-beta-binding protein 1
  • DNA topoisomerase II-binding protein 1
  • KIAA0259DNA topoisomerase 2-binding protein 1
  • TOP2BP1
  • TopBP1
  • topoisomerase (DNA) II binding protein 1

Background

Topoisomerases regulate topological states of DNA. DNA topoisomerase II-binding protein 1 (TopBP1) is a protein that interacts with the C-terminal region of topoisomerase II beta (Topo II beta). TopBP1 function has been found to be important to various aspects of cellular growth control such as DNA replication, the DNA damage checkpoint, and cell survival. TopBP1 interacts with several proteins through its BRCA1 C-terminal (BRCT) motifs. TopBP1 interacts and inhibits E2F1 transcriptional activity through an Rb-independent mechanism that involves recruitment of the SWI/SNF chromatin-remodeling complex proteins Brg1 and Brm. TopBP1 is also known as DNA topoisomerase 2-binding protein, DNA topoisomerase II beta-binding protein 1, TOPBP1, and KIAA0259.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TopBP1 Antibody (NB100-217)(17)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, WB.


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IP
(1)
WB
(5)
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Human
(5)
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Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB100-217 Applications Species
Choe KN, Nicolae CM, Constantin D et al. HUWE1 interacts with PCNA to alleviate replication stress. EMBO Rep. May 4 2016 [PMID: 27146073] (WB, Human) WB Human
Tomida J, Itaya A, Shigechi T et al. A novel interplay between the Fanconi anemia core complex and ATR-ATRIP kinase during DNA cross-link repair. Nucleic Acids Res 2013 May 30 [PMID: 23723247] (WB, Human) WB Human
Ohlenschlager O, Kuhnert A, Schneider A et al. The N-terminus of the human RecQL4 helicase is a homeodomain-like DNA interaction motif Nucleic Acids Res 2012 Jun 22 [PMID: 22730300] (IP, WB, Human) IP, WB Human
Lee Y, Katyal S, Downing SM et al. Neurogenesis requires TopBP1 to prevent catastrophic replicative DNA damage in early progenitors. Nat Neurosci 2012 Jun [PMID: 22522401]
Kuhnert A, Schmidt U, Monajembashi S et al. Proteomic identification of PSF and p54(nrb) as topBP1-interacting proteins J. Cell. Biochem 2012 [PMID: 22213094] (WB, Human) WB Human
Kumagai A, Shevchenko A, Shevchenko A et al. Direct regulation of Treslin by cyclin-dependent kinase is essential for the onset of DNA replication. J Cell Biol 2011 Jun [PMID: 21646402]
Forrester NA, Sedgwick GG, Thomas A et al. Serotype-specific inactivation of the cellular DNA damage response during adenovirus infection. J Virol 2011 Mar [PMID: 21159879]
Blackford AN, Patel RN, Forrester NA et al. Adenovirus 12 E4orf6 inhibits ATR activation by promoting TOPBP1 degradation. Proc Natl Acad Sci U S A 2010 Jul [PMID: 20566845]
Guo R, Chen J, Zhu F et al. E2F1 localizes to sites of UV-induced DNA damage to enhance nucleotide excision repair. J Biol Chem 2010 Jun [PMID: 20413589]
Sansam CL, Cruz NM, Danielian PS et al. A vertebrate gene, ticrr, is an essential checkpoint and replication regulator. Genes Dev 2010 Jan 15 [PMID: 20080954] (WB, Human) WB Human
Show All 17 Publications.

Review for TopBP1 Antibody (NB100-217) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-217:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot TopBP1 NB100-217
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reviewed by:
Anonymous
WB Human 07/21/2010
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Summary

ApplicationWestern Blot
Sample TestedHeLa Cell Extract - Mitotic and Interphase, Sample Amount: Approximately 5x10^6 cell equivalents/lane
SpeciesHuman
CommentsThe antibody worked to detect a protein the size of TopBP1 (~180kDa) in Hela cell extract (interphase and mitotic) but also recognized a band of a smaller size (~95kDa) and with much more intensity.

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat dry milk in PBST, Blocking Time: 45 minutes, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioDilution Ratio: 1:2,000, Incubation Dilution Buffer: 5% non-fat dry milk in PBST, Incubation Time: 15 hours, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat-anti-Rb-HRP in 5% non-fat dry milk/PBST, Secondary Ab Dilution Ratio: 1:20,000
Secondary Manufacturer Cat#Jackson ImmunoResearch Cat #111-035-144

Details

Detection NotesMethod: SuperSignal West Pico Chemiluminescent Substrate, Exposure Time: 5 min, Bands: 180 kDa(specific), 95 kDa(non-specific)

Comments

CommentsThe antibody worked to detect a protein the size of TopBP1 (~180kDa) in Hela cell extract (interphase and mitotic) but also recognized a band of a smaller size (~95kDa) and with much more intensity.

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Recent Reviews

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Anonymous
07/21/2010
Application: WB
Species: Human

Bioinformatics

Gene Symbol TOPBP1
Entrez
Uniprot