PLK1 Antibody (OTI1D4)

Images

 
Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291] - (Negative control: IP without adding anti-PLK1 antibody.). For each experiment, 500ul of DDK tagged PLK1 overexpression lysates (at 1:5 dilution with HEK293T lysate), ...read more
Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded human endometrium tissue using PLK1 antibody (OTI1D4). Primary antibody dilution 1:200. Image from verified customer ...read more
Flow Cytometry: PLK1 Antibody (OTI1D4) [NBP1-48291] - HEK293T cells transfected with either PLK1 Human Tagged ORF Clone overexpress plasmid (Red), compared to an IgG isotype control, (Green) or empty vector control ...read more
Western Blot: PLK1 Antibody (OTI1D4) [NBP1-48291] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PLK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PLK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PLK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-PLK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human endometrium tissue using anti-PLK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human pancreas tissue using anti-PLK1 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: PLK1 Antibody (OTI1D4) [NBP1-48291] - Staining of paraffin-embedded Human prostate tissue using anti-PLK1 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, IHC, IHC-P, IP
Clone
OTI1D4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
2.5 mg/ml

PLK1 Antibody (OTI1D4) Summary

Immunogen
Full length human recombinant protein of human PLK1 (NP_005021) produced in HEK293T cell.
Marker
Mitosis Marker
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PLK1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Immunoprecipitation 2ug/500ul
  • Western Blot 1:2000
Theoretical MW
68.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-48291 in the following applications:

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
2.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PLK1 Antibody (OTI1D4)

  • cell cycle regulated protein kinase
  • EC 2.7.11
  • EC 2.7.11.21
  • PLK
  • PLK1
  • PLK-1
  • polo (Drosophia)-like kinase
  • polo like kinase
  • polo-like kinase 1polo-like kinase (Drosophila)
  • Serine/threonine-protein kinase 13
  • serine/threonine-protein kinase PLK1
  • STPK13

Background

Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PLK1 Antibody (NBP1-48291) (0)

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Review for PLK1 Antibody (NBP1-48291) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-48291:
Filter by Applications
Immunohistochemistry-Paraffin
(1)
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Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin PLK1 NBP1-48291
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5
reviewed by:
Tatyana Martynova
Immunohistochemistry-Paraffin Mouse 12/18/2021
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedEndometrium
SpeciesMouse
LotOTI1D4

Comments

CommentsPrimary antibody dilution 1:200, secondary antibody 1:300

Product General Protocols

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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1
4
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2
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1
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Tatyana Martynova
12/18/2021
Application: Immunohistochemistry-Paraffin
Species: Mouse

Bioinformatics

Gene Symbol PLK1
Entrez
Uniprot