Claspin Antibody - BSA Free

Western Blot: Claspin Antibody [NB100-246] - Detection of Human Claspin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Claspin antibody NB100-246 used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Western Blot: Claspin Antibody [NB100-246] - Whole cell lysate (20 ug) from HeLa cells. Antibody used at 1 or 0.5 ug/ml with (+) or without (-) blocking peptide.

• Reactivity: Hu
• Applications: WB, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Claspin Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 450 and 500 of human Claspin using the numbering given in entry AAG24515.1 (GeneID 63967).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CLSPN
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:5000
• Application Notes: By Western blot, this antibody recognizes bands around 175 kDa representing Claspin. Not suitable for Immunoprecipitation. *The investigator should determine the optimal working dilution for a specific application. **Use NB 100-247 and/or NB 100-248 for Immunoprecipitation.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Claspin Antibody - BSA Free

• claspin homolog (Xenopus laevis)
• claspin homolog
• Claspin
• CLSPN
• hClaspin
• MGC131612
• MGC131613
• MGC131615

Background

Claspin is a cell cycle regulated protein. It plays a critical role in replication checkpoint control and interacts with the checkpoint proteins ATR and Rad9. It also associates with Chk1, but only following replication stress or other types of DNA damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Claspin Antibody (NB100-246)(4)

Publications using NB100-246

• Lavik AR, McColl KS, Lemos FO et al. A non-canonical role for pyruvate kinase M2 as a functional modulator of Ca2+ signalling through IP3 receptors Biochimica et biophysica acta. Molecular cell research 2022-01-11 [PMID: 35026348] (IP, Human)
• Yoshizawa-Sugata N, Masai H. Human Tim/Timeless-interacting protein, Tipin, is required for efficient progression of S phase and DNA replication checkpoint. J Biol Chem 2007-01-01 [PMID: 17102137]
• Rodriguez-Bravo V, Guaita-Esteruelas S, Florensa R et al. Chk1- and claspin-dependent but ATR/ATM- and Rad17-independent DNA replication checkpoint response in HeLa cells. Cancer Res 2006-09-01 [PMID: 16951182]
• Jiang K, Pereira E, Maxfield M et al. Regulation of Chk1 includes chromatin association and 14-3-3 binding following phosphorylation on Ser-345. J Biol Chem 2003-07-01 [PMID: 12676962]

Bioinformatics

• Gene Symbol: CLSPN
• Entrez:
  • 63967(Human)
• Uniprot:
  • AAG24515.1(Human)