CHD7 Antibody [Biotin]

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

CHD7 Antibody [Biotin] Summary

Immunogen
A genomic peptide made to an N-terminal region of the human Chd7 protein (within residues 25-200). [Swiss-Prot Q9P2D1]
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHD7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This CHD7 antibody is useful for IHC-P, ICC/IF and Western blot where a band is seen ~330 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Readout System

Reactivity Notes

Human and mouse. Immunogen has 85% identity to chicken Chd7.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CHD7 Antibody [Biotin]

  • ATP-dependent helicase CHD7
  • CHD7
  • CHD-7
  • chromodomain helicase DNA binding protein 7 isoform CRA_e
  • chromodomain helicase DNA binding protein 7
  • chromodomain-helicase-DNA-binding protein 7
  • EC 3.6.1
  • EC 3.6.4.12
  • FLJ20357
  • FLJ20361
  • IS3
  • KIAA1416KAL5

Background

CHARGE syndrome (coloboma, heart defects, atresia of the choanae, retarded growth and development, genital hypoplasia, ear anomalies and deafness) is a congenital malformation syndrome caused by mutations in the CHD7 (chromodomain helicase DNA-binding protein) gene in approximately 2/3 of cases. In Kallmann syndrome, a similar proportion of affected individuals also have mutated CHD7. These mutations probably affect neurogenerative anomalies and maturation events through SOX2 interaction. Expression patterns of CHD7 in combination with SOX2 evaluation can provide some insight into molecular causes of CHARGE and Kallmann syndromes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional CHD7 Products

CHD7 NBP1-77393B

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Bioinformatics

Gene Symbol CHD7
Entrez