CHD7 Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Chd7 Antibody [NBP1-77393] - Antibody was tested in NIH/3T3 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: Chd7 Antibody [NBP1-77393] - IHC analysis of CHD7 in mouse intestine using DAB with hematoxylin counterstain.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.27 mg/ml

CHD7 Antibody Summary

Immunogen
A genomic peptide made to an N-terminal region of the human Chd7 protein (within residues 25-200). [Swiss-Prot Q9P2D1]
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CHD7
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2500
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Paraffin 1:250
Application Notes
This CHD7 antibody is useful for IHC-P, ICC/IF and Western blot where a band is seen ~330 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Positive Control
NIH 3T3 Lysate (NB800-PC8)
Reviewed Applications
Read 1 Review rated 5
using
NBP1-77393 in the following applications:

Publications
Read Publication using
NBP1-77393 in the following applications:

  • 1 publication
  • WB
    1 publication

Reactivity Notes

Human and mouse. Immunogen has 85% identity to chicken Chd7.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1.27 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for CHD7 Antibody

  • ATP-dependent helicase CHD7
  • CHD7
  • CHD-7
  • chromodomain helicase DNA binding protein 7 isoform CRA_e
  • chromodomain helicase DNA binding protein 7
  • chromodomain-helicase-DNA-binding protein 7
  • EC 3.6.1
  • EC 3.6.4.12
  • FLJ20357
  • FLJ20361
  • IS3
  • KIAA1416KAL5

Background

CHARGE syndrome (coloboma, heart defects, atresia of the choanae, retarded growth and development, genital hypoplasia, ear anomalies and deafness) is a congenital malformation syndrome caused by mutations in the CHD7 (chromodomain helicase DNA-binding protein) gene in approximately 2/3 of cases. In Kallmann syndrome, a similar proportion of affected individuals also have mutated CHD7. These mutations probably affect neurogenerative anomalies and maturation events through SOX2 interaction. Expression patterns of CHD7 in combination with SOX2 evaluation can provide some insight into molecular causes of CHARGE and Kallmann syndromes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-77393
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for CHD7 Antibody (NBP1-77393)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-P, WB.


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IHC-P
(1)
WB
(1)
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Human
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Review for CHD7 Antibody (NBP1-77393) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: ICC.

Reviews using NBP1-77393:
Filter by Applications
ICC
(1)
All Applications
Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry CHD7 NBP1-77393
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reviewed by:
Valentina André
ICC Mouse 09/28/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedGN11 cell line
SpeciesMouse
LotA-1

Comments

CommentsFixation: 4% paraformaldehyde in PBS pH 7.4 for 15 minutes at room temperature
Permeabilization: PBS containing 0.2% Triton X-100 for 10 minutes
Blocking: PBST (PBS+ 0.1% Tween 20) 10% goat serum for 60 minutes
Immunostaining: primary Ab 1:100 in PBST in a humidified chamber overnight at 4°C

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CHD7 Antibody (NBP1-77393). (Showing 1 - 1 of 1 FAQ).

  1. I am working with Chd7 and have just visited your website. I would like to have some advices or protocols to use the NBP1-77393 Ab, especially western blot protocol/extraction buffers. My concern is that Chd7 is a high molecular weight protein and it seems very difficult to extract it and to transfer it on a membrane I was just wondering if you had any tips regarding this matter
    • It was a 5% gel ran 100V for 60 min stressing that it was done in cold conditions with ice packs. It should be ok to do overnight at a lower voltage also in cold conditions. We had the best success on NIH3T3 cells, but also tested on Jurkat. In regards to your inquiry about the Western Blot protocol specific for NBP1-77393, we do have one available on our website. You can find this under the protocol tab. In case you have trouble locating this I will provide it to you in this email. These are just suggestions and some additional optimization may need to be done to achieve the best results based on sample preparation and protein expression. Western Blot Protocol: 1. Perform SDS-PAGE on samples to be analyzed, loading 40 ug of total protein per lane. 2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus. 3. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate. 4. Rinse the blot. 5. Block the membrane using standard blocking buffer for at least 1 hour. 6. Wash the membrane in wash buffer three times for 10 minutes each. 7. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature. 8. Wash the membrane in wash buffer three times for 10 minutes each. 9. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature. 10. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background). 11. Apply the detection reagent of choice in accordance with the manufacturers instructions. Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2%.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-77393AF405
Alexa Fluor 488 Labeled NBP1-77393AF488
Alexa Fluor 647 Labeled NBP1-77393AF647
Alexa Fluor 700 Labeled NBP1-77393AF700
Biotin Labeled NBP1-77393B
DyLight 405 Labeled NBP1-77393V
DyLight 488 Labeled NBP1-77393G
DyLight 550 Labeled NBP1-77393R
DyLight 650 Labeled NBP1-77393C
FITC Labeled NBP1-77393F
HRP Labeled NBP1-77393H

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CHD7 NBP1-77393

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Recent Reviews

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Valentina André
09/28/2017
Application: ICC
Species: Mouse

Bioinformatics

Gene Symbol CHD7
Entrez
Uniprot