Neurokinin B Antibody

Images

 
Immunohistochemistry: Neurokinin B Antibody [NB300-201] - Detection of Neurokinin B in rat brain tissue (Alexa 488 stain), using NB 300-201.
Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:200 dilution. The antibody depicted an ...read more
Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:100 dilution. The antibody showed a ...read more
Immunohistochemistry: Neurokinin B Antibody [NB300-201] - IF analysis of Neurokinin B in mouse hypothalamus. Image courtesy of an anonymous customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Neurokinin B Antibody Summary

Immunogen
A synthetic peptide made to an internal sequence of mouse proNeurokinin B (~93% homology to the rat sequence). [UniProt# P55099]
Localization
Secreted
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TAC3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Application Notes
This Neurokinin B antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded and frozen sections. This antibody has been used on human hypothalamus and rat brain sections. In IHC, positive staining of the arcuate nucleus is seen. There has been customer feedback that this antibody works in Western blot; please note that this is a secreted protein.
Positive Control
Neurokinin B Lysate (NBL1-16669)
Control Peptide
Neurokinin B Peptide (NB300-201PEP)
Reviewed Applications
Read 2 Reviews rated 4
using
NB300-201 in the following applications:

Publications
Read Publications using
NB300-201 in the following applications:

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Neurokinin B Antibody

  • gamma tachykinin 3
  • neurokinin beta
  • neurokinin B-like
  • neuromedin K
  • preprotachykinin B
  • PRO1155
  • tachykinin 3
  • tachykinin-3
  • ZNEUROK1NKNBNKB

Background

Neurokinin B (NKB) belongs to tachykinin family of peptides that also includes substance P, NKA, neuropeptide K, neuropeptide-gamma, and hemokinin-1, and NKB is the only tachykinin synthesized from preprotachykinin-B gene (designated as TAC3 in mammals, except for rodents where it is named Tac2). Tachykinins are characterized by a common carboxyl-terminal amino acid sequence of FXGLM-NH2, X being a hydrophobic residue and are active peptides which stimulates neurons, evoke behavioral responses, are potent vasodilators and secretagogues (secretion stimulators), and contract directly or indirectly smooth muscles. NKB is the cleaved product of neurokinin B-like protein Zneurok1 (also called preprotachykinin-B or tachykinin-3) and act as an endogenous agonist ligand for its receptor, the NK3 receptor (NKB receptor). NKB signaling has been reported to play critical role in pre-eclampsia which is a potentially life threatening disease and is essential to neuroprotection in neurodegenerative diseases as well as to normal reproductive development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neurokinin B Antibody (NB300-201)(15)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-Fr.


Filter By Application
ICC/IF
(1)
IHC
(4)
IHC-Fr
(5)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
Rat
(9)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB300-201 Applications Species
Kanaya M, Iwata K, Ozawa H. Distinct dynorphin expression patterns with low- and high-dose estrogen treatment in the arcuate nucleus of female rats. Biol. Reprod. 2017 Oct 24 [PMID: 29069289] (Rat) Rat
Osuka S, Iwase A, Nakahara T et al. Kisspeptin in the hypothalamus of two rat models of polycystic ovary syndrome. Endocrinology. Dec 16 2016 [PMID: 27983870] (Rat) Rat
Obata K, Shimo T, Okui T et al. Tachykinin Receptor 3 Distribution in Human Oral Squamous Cell Carcinoma. Anticancer Res. Dec 1 2016 [PMID: 27919954] (IHC, Human) IHC Human
Kunimura Y, Iwata K, Ishigami A, Ozawa H. Age-related alterations in hypothalamic kisspeptin, neurokinin B, and dynorphin neurons and in pulsatile LH release in female and male rats. Neurobiol. Aging. Oct 19 2016 [PMID: 27842268] (IHC, Rat) IHC Rat
Mittelman-Smith MA, Krajewski-Hall SJ, McMullen NT, Rance NE Ablation of KNDy Neurons Results in Hypogonadotropic Hypogonadism and Amplifies the Steroid-Induced LH Surge in Female Rats. Endocrinology 2016 May [PMID: 26937713]
Pineda Reyes R, Sabatier N, Ludwig M et al. A direct neurokinin B projection from the arcuate nucleus regulates magnocellular vasopressin cells of the supraoptic nucleus. J. Neuroendocrinol. 2015 Nov 27 [PMID: 26610724] (IHC-Fr, Rat) IHC-Fr Rat
Helena CV, Toporikova N, Kalil B et al. KNDy neurons modulate the magnitude of the steroid-induced luteinizing hormone surges in ovariectomized rats. Endocrinology 2015 Aug 24 [PMID: 26302111]
Mittelman-Smith MA, Krajewski-Hall SJ, McMullen NT, Rance NE. Neurokinin 3 Receptor-Expressing Neurons in the Median Preoptic Nucleus Modulate Heat-Dissipation Effectors in the Female Rat Endocrinology 2015 Mar 31 [PMID: 25825817] (IHC-Fr, Rat) IHC-Fr Rat
True C, Nasrin Alam S, Cox K et al. Neurokinin B is critical for normal timing of sexual maturation but dispensable for adult reproductive function in female mice. Endocrinology 2015 Apr [PMID: 25574869] (Mouse) Mouse
Cholanian M, Krajewski-Hall SJ, Levine RB et al. Electrophysiology of Arcuate Neurokinin B Neurons in Female Tac2-EGFP Transgenic Mice. Endocrinology 2014 Apr 15 [PMID: 24735328] (IHC-Fr, Mouse) IHC-Fr Mouse
Show All 15 Publications.

Reviews for Neurokinin B Antibody (NB300-201) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.
We have 2 reviews tested in 2 application: IHC, IF.

Reviews using NB300-201:
Filter by Applications
IHC
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry Neurokinin B NB300-201
Enlarge
reviewed by:
Andree Lessard
IHC Rat 06/22/2012
View

Summary

ApplicationImmunohistochemistry
Sample Testedrat tissue fixed with PFA and acrolein
SpeciesRat
Commentsgood labeling, minimal background, excellent specificity where a single band could be seen by western blot in cells transfected with the NK3 DNA; no bands in cells not transfected or transfected with vector alone...

Blocking

Blocking Detailsblcking agent 0.5% BSA-TS for 30 mins

Primary Anitbody

Dilution Ratio1:1000, 12hr room temperature in 0.1% BSA-TS

Secondary Antibody

Secondary Descriptiongoat anti-rabbit biotin conjugated
Secondary Manufacturer Cat#Jacksons
Secondary Concentration1:400

Details

Detection NotesDAb incubation 6 mins
Fixation DetailsPFA + acrolein

Comments

Commentsgood labeling, minimal background, excellent specificity where a single band could be seen by western blot in cells transfected with the NK3 DNA; no bands in cells not transfected or transfected with vector alone...
Immunofluorescence Neurokinin B NB300-201
Enlarge
reviewed by:
Christina Merkley
IF Mouse 05/15/2012
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse hypothalamus
SpeciesMouse
LotA3
CommentsUsed in a dual- fluorescent protocol, we chose to amplify the NKB. The staining is strong and crisp in mouse tissue. For the amplification we used: Biotinylated goat anti- rabbit followed by ABC, Biotinylated Tyramide, and incubation in streptavidin (with rinses in PBS in between each step).

Blocking

Blocking Details1% H202 solution (in PBS) for 10 min at RT, and also block in PBS + 0.04% Triton X-100 + 0.1% BSA for 1h at RT before primary.

Primary Anitbody

Dilution Ratio1:5000 for 17 h at RT, diluted in PBS with 0.04% Triton X-100 and 1% BSA.

Secondary Antibody

Secondary DescriptionBiotinylated goat anti- Rabbit (Vector Labs) in PBS with Triton X and BSA.
Secondary Manufacturer Cat#BA-1000
Secondary Concentration1:500

Details

Detection NotesDetected using TSA Amplification, and stained using Cy5- streptavidin (1:100). Pos control: peptide preabsorption
Fixation Details4% PFA Fixation
Wash DescriptionPBS washes between each step. 4 x 5 minutes

Comments

CommentsUsed in a dual- fluorescent protocol, we chose to amplify the NKB. The staining is strong and crisp in mouse tissue. For the amplification we used: Biotinylated goat anti- rabbit followed by ABC, Biotinylated Tyramide, and incubation in streptavidin (with rinses in PBS in between each step).

Product General Protocols

View specific protocols for Neurokinin B Antibody (NB300-201): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Neurokinin B Antibody (NB300-201). (Showing 1 - 1 of 1 FAQs).

  1. Has NB300-201 been tested by IHC on frozen of paraffin embedded tissue section?
    • This antibody is suitable for use on both frozen and paraffin-embedded tissues.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB300-201AF405
Alexa Fluor 488 Labeled NB300-201AF488
Alexa Fluor 647 Labeled NB300-201AF647
Alexa Fluor 700 Labeled NB300-201AF700
Biotin Labeled NB300-201B
DyLight 405 Labeled NB300-201V
DyLight 488 Labeled NB300-201G
DyLight 550 Labeled NB300-201R
DyLight 650 Labeled NB300-201C
FITC Labeled NB300-201F
HRP Labeled NB300-201H

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Andree Lessard
06/22/2012
Application: IHC
Species: Rat

Christina Merkley
05/15/2012
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol TAC3
Entrez
Uniprot