Neurokinin B Antibody - BSA Free

Immunohistochemistry: Neurokinin B Antibody - BSA Free [NB300-201] - Immunofluorescence photomicrograph showing that the expression of Neurokinin B in the arcuate nucleus (ARC). Scale bars: 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32921318/) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:200 dilution. The antibody depicted an expected strong cytoplasmic staining in the neuronal cells.

Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:100 dilution. The antibody showed a specific cytoplasmic staining in the Purkinje cells and some granular neurons from the granular layer with weak signal in the fibers within the molecular layer of the section.

Immunohistochemistry: Neurokinin B Antibody [NB300-201] - IF analysis of Neurokinin B in mouse hypothalamus. Image courtesy of an anonymous customer review.

Immunohistochemistry: Neurokinin B Antibody [NB300-201] - Detection of Neurokinin B in rat brain tissue (Alexa 488 stain), using NB 300-201.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Neurokinin B Antibody - BSA Free Summary

• Immunogen: A synthetic peptide made to an internal sequence of mouse proNeurokinin B (~93% homology to the rat sequence). [UniProt# P55099]
• Localization: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TAC3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:100-1:2000
• Application Notes: This Neurokinin B antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded and frozen sections. This antibody has been used on human hypothalamus and rat brain sections. In IHC, positive staining of the arcuate nucleus is seen. There has been customer feedback that this antibody works in Western blot; please note that this is a secreted protein.
• Control:

  Neurokinin B Overexpression Lysate

• Control Peptide:

  Neurokinin B Peptide (NB300-201PEP)

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Neurokinin B Antibody - BSA Free

• gamma tachykinin 3
• neurokinin beta
• neurokinin B-like
• neuromedin K
• preprotachykinin B
• PRO1155
• tachykinin 3
• tachykinin-3
• ZNEUROK1NKNBNKB

Background

Neurokinin B (NKB) belongs to tachykinin family of peptides that also includes substance P, NKA, neuropeptide K, neuropeptide-gamma, and hemokinin-1, and NKB is the only tachykinin synthesized from preprotachykinin-B gene (designated as TAC3 in mammals, except for rodents where it is named Tac2). Tachykinins are characterized by a common carboxyl-terminal amino acid sequence of FXGLM-NH2 , X being a hydrophobic residue and are active peptides which stimulates neurons, evoke behavioral responses, are potent vasodilators and secretagogues (secretion stimulators), and contract directly or indirectly smooth muscles. NKB is the cleaved product of neurokinin B-like protein Zneurok1 (also called preprotachykinin-B or tachykinin-3) and act as an endogenous agonist ligand for its receptor, the NK3 receptor (NKB receptor). NKB signaling has been reported to play critical role in pre-eclampsia which is a potentially life threatening disease and is essential to neuroprotection in neurodegenerative diseases as well as to normal reproductive development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neurokinin B Antibody (NB300-201)(24)

Publications using NB300-201

• Roberts SA, NaulE L, Chouman S et al. Hypothalamic Overexpression of Makorin Ring Finger Protein 3 Results in Delayed Puberty in Female Mice Endocrinology 2022-08-17 [PMID: 35974456]
• Senft RA, Freret ME, Sturrock N, Dymecki SM Neurochemically and hodologically distinct ascending VGLUT3 versus serotonin subsystems comprise the r2-Pet1 median raphe The Journal of neuroscience : the official journal of the Society for Neuroscience 2021-02-05 [PMID: 33547164]
• Liu, X, Yeo, S H Et al. Highly redundant neuropeptide volume co-transmission underlying episodic activation of the GnRH neuron dendron. Elife 2021-01-19 [PMID: 33464205] (WB, Human)
• Chen S, Gao XF, Zhou Y et al. A spinal neural circuitry for converting touch to itch sensation Nat Commun 2020-10-08 [PMID: 33033265] (IHC-Fr, Mouse)
• Gao Z, Ma X, et al. Troxerutin protects against DHT-induced polycystic ovary syndrome in rats. J Ovarian Res 2020-09-13 [PMID: 32921318] (IF/IHC, Rat)
• Dees WL, Hiney JK, Srivastava VK Regulation of prepubertal dynorphin secretion in the medial basal hypothalamus of the female rat J. Neuroendocrinol. 2019-12-01 [PMID: 31715027] (WB, Rat)
• Sakata D, Uruno T et al. Selective role of neurokinin B in IL-31-induced itch response in mice. J Allergy Clin Immunol 2019-01-10 [PMID: 31405606] (IF/IHC, Mouse)
• Fergani C, Leon S, Padilla SL et al. NKB signaling in the posterodorsal medial amygdala stimulates gonadotropin release in a kisspeptin-independent manner in female mice Elife 2018-12-19 [PMID: 30565563] (IHC-Fr, Mouse)
• Srivastava Vinod K, Hiney Jill K, Dees William L et al. Alcohol Delays the Onset of Puberty in the Female Rat by Altering Key Hypothalamic Events. Alcoholism, Clinical and Experimental Research 2018-07-01 [PMID: 29689132] (WB, Rat)
• Kanaya M, Iwata K, Ozawa H. Distinct dynorphin expression patterns with low- and high-dose estrogen treatment in the arcuate nucleus of female rats. Biol. Reprod. 2017-10-24 [PMID: 29069289] (Rat)
• Osuka S, Iwase A, Nakahara T et al. Kisspeptin in the hypothalamus of two rat models of polycystic ovary syndrome. Endocrinology. 2016-12-16 [PMID: 27983870] (Rat)
• Obata K, Shimo T, Okui T et al. Tachykinin Receptor 3 Distribution in Human Oral Squamous Cell Carcinoma. Anticancer Res. 2016-12-01 [PMID: 27919954] (IF/IHC, Human)
• Kunimura Y, Iwata K, Ishigami A, Ozawa H. Age-related alterations in hypothalamic kisspeptin, neurokinin B, and dynorphin neurons and in pulsatile LH release in female and male rats. Neurobiol. Aging. 2016-10-19 [PMID: 27842268] (IF/IHC, Rat)
• Mittelman-Smith MA, Krajewski-Hall SJ, McMullen NT, Rance NE Ablation of KNDy Neurons Results in Hypogonadotropic Hypogonadism and Amplifies the Steroid-Induced LH Surge in Female Rats. Endocrinology 2016-05-01 [PMID: 26937713]
• Pineda Reyes R, Sabatier N, Ludwig M et al. A direct neurokinin B projection from the arcuate nucleus regulates magnocellular vasopressin cells of the supraoptic nucleus. J. Neuroendocrinol. 2015-11-27 [PMID: 26610724] (IHC-Fr, Rat)
• Helena CV, Toporikova N, Kalil B et al. KNDy neurons modulate the magnitude of the steroid-induced luteinizing hormone surges in ovariectomized rats. Endocrinology 2015-08-24 [PMID: 26302111]
• Mittelman-Smith MA, Krajewski-Hall SJ, McMullen NT, Rance NE. Neurokinin 3 Receptor-Expressing Neurons in the Median Preoptic Nucleus Modulate Heat-Dissipation Effectors in the Female Rat Endocrinology 2015-03-31 [PMID: 25825817] (IHC-Fr, Rat)
• True C, Nasrin Alam S, Cox K et al. Neurokinin B is critical for normal timing of sexual maturation but dispensable for adult reproductive function in female mice. Endocrinology 2015-04-01 [PMID: 25574869] (Mouse)
• Cholanian M, Krajewski-Hall SJ, Levine RB et al. Electrophysiology of Arcuate Neurokinin B Neurons in Female Tac2-EGFP Transgenic Mice. Endocrinology 2014-04-15 [PMID: 24735328] (IHC-Fr, Mouse)
• Mittelman-Smith MA, Williams H, Krajewski-Hall SJ, Lai J, Ciofi P, McMullen NT, Rance NE. Arcuate Kisspeptin/Neurokinin B/Dynorphin (KNDy) Neurons Mediate the Estrogen Suppression of Gonadotropin Secretion and Body Weight. Endocrinology;153(6):2800-12. 2012-06-01 [PMID: 22508514] (IF/IHC, Rat)
• Krajewski SJ, Burke MC, Anderson MJ et al. Forebrain projections of arcuate neurokinin B neurons demonstrated by anterograde tract-tracing and monosodium glutamate lesions in the rat. Neuroscience. 2010-03-17 [PMID: 20038444] (ICC/IF, IF/IHC, Rat)
• Halasz J, Zelena D, Toth M et al. Substance P neurotransmission and violent aggression: the role of tachykinin NK(1) receptors in the hypothalamic attack area. Eur J Pharmacol. 2009-06-02 [PMID: 19344710] (IHC-Fr, Rat)
• Burke MC, Letts PA, Krajewski SJ, Rance NE. Coexpression of dynorphin and neurokinin B immunoreactivity in the rat hypothalamus: Morphologic evidence of interrelated function within the arcuate nucleus. J Comp Neurol;498(5):712-26. 2006-10-10 [PMID: 16917850]
• Krajewski SJ, Anderson MJ, Iles-Shih L Morphologic evidence that neurokinin B modulates gonadotropin-releasing hormone secretion via neurokinin 3 receptors in the rat median eminence. J Comp Neurol. 2005-08-29 [PMID: 16025449] (IHC-Fr, Rat)

Reviews for Neurokinin B Antibody (NB300-201) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Rat.

reviewed by: Verified Customer IHC Rat 06/22/2012

Summary

• Application: Immunohistochemistry
• Sample Tested: rat tissue fixed with PFA and acrolein
• Species: Rat

reviewed by: Verified Customer IF Mouse 05/15/2012

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse hypothalamus
• Species: Mouse
• Lot: A3

FAQs for Neurokinin B Antibody (NB300-201). (Showing 1 - 1 of 1 FAQs).

1. Has NB300-201 been tested by IHC on frozen of paraffin embedded tissue section?
   • This antibody is suitable for use on both frozen and paraffin-embedded tissues.

Bioinformatics

• Gene Symbol: TAC3
• Entrez:
  • 6866(Human)
  • 21334(Mouse)
  • 29191(Rat)
• Uniprot:
  • P55099(Mouse)