Neurokinin B Antibody

Immunohistochemistry: Neurokinin B Antibody [NB300-201] - Detection of Neurokinin B in rat brain tissue (Alexa 488 stain), using NB 300-201.

Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:200 dilution. The antibody depicted an expected strong cytoplasmic staining in the neuronal cells.

Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:100 dilution. The antibody showed a specific cytoplasmic staining in the Purkinje cells and some granular neurons from the granular layer with weak signal in the fibers within the molecular layer of the section.

Immunohistochemistry: Neurokinin B Antibody [NB300-201] - IF analysis of Neurokinin B in mouse hypothalamus. Image courtesy of an anonymous customer review.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Neurokinin B Antibody Summary

• Immunogen: A synthetic peptide made to an internal sequence of mouse proNeurokinin B (~93% homology to the rat sequence). [UniProt# P55099]
• Localization: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TAC3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
• Application Notes: This Neurokinin B antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded and frozen sections. This antibody has been used on human hypothalamus and rat brain sections. In IHC, positive staining of the arcuate nucleus is seen. There has been customer feedback that this antibody works in Western blot; please note that this is a secreted protein.
• Positive Control:

  Neurokinin B Lysate (NBL1-16669)

• Control Peptide:

  Neurokinin B Peptide (NB300-201PEP)

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Neurokinin B Antibody

• gamma tachykinin 3
• neurokinin beta
• neurokinin B-like
• neuromedin K
• preprotachykinin B
• PRO1155
• tachykinin 3
• tachykinin-3
• ZNEUROK1NKNBNKB

Background

Neurokinin B (NKB) belongs to tachykinin family of peptides that also includes substance P, NKA, neuropeptide K, neuropeptide-gamma, and hemokinin-1, and NKB is the only tachykinin synthesized from preprotachykinin-B gene (designated as TAC3 in mammals, except for rodents where it is named Tac2). Tachykinins are characterized by a common carboxyl-terminal amino acid sequence of FXGLM-NH2, X being a hydrophobic residue and are active peptides which stimulates neurons, evoke behavioral responses, are potent vasodilators and secretagogues (secretion stimulators), and contract directly or indirectly smooth muscles. NKB is the cleaved product of neurokinin B-like protein Zneurok1 (also called preprotachykinin-B or tachykinin-3) and act as an endogenous agonist ligand for its receptor, the NK3 receptor (NKB receptor). NKB signaling has been reported to play critical role in pre-eclampsia which is a potentially life threatening disease and is essential to neuroprotection in neurodegenerative diseases as well as to normal reproductive development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Neurokinin B Antibody (NB300-201)(15)

Publications using NB300-201

• Kanaya M, Iwata K, Ozawa H. Distinct dynorphin expression patterns with low- and high-dose estrogen treatment in the arcuate nucleus of female rats. Biol. Reprod. 2017 Oct 24 [PMID: 29069289] (Rat)
• Osuka S, Iwase A, Nakahara T et al. Kisspeptin in the hypothalamus of two rat models of polycystic ovary syndrome. Endocrinology. Dec 16 2016 [PMID: 27983870] (Rat)
• Obata K, Shimo T, Okui T et al. Tachykinin Receptor 3 Distribution in Human Oral Squamous Cell Carcinoma. Anticancer Res. Dec 1 2016 [PMID: 27919954] (IHC, Human)
• Kunimura Y, Iwata K, Ishigami A, Ozawa H. Age-related alterations in hypothalamic kisspeptin, neurokinin B, and dynorphin neurons and in pulsatile LH release in female and male rats. Neurobiol. Aging. Oct 19 2016 [PMID: 27842268] (IHC, Rat)
• Mittelman-Smith MA, Krajewski-Hall SJ, McMullen NT, Rance NE Ablation of KNDy Neurons Results in Hypogonadotropic Hypogonadism and Amplifies the Steroid-Induced LH Surge in Female Rats. Endocrinology 2016 May [PMID: 26937713]
• Pineda Reyes R, Sabatier N, Ludwig M et al. A direct neurokinin B projection from the arcuate nucleus regulates magnocellular vasopressin cells of the supraoptic nucleus. J. Neuroendocrinol. 2015 Nov 27 [PMID: 26610724] (IHC-Fr, Rat)
• Helena CV, Toporikova N, Kalil B et al. KNDy neurons modulate the magnitude of the steroid-induced luteinizing hormone surges in ovariectomized rats. Endocrinology 2015 Aug 24 [PMID: 26302111]
• Mittelman-Smith MA, Krajewski-Hall SJ, McMullen NT, Rance NE. Neurokinin 3 Receptor-Expressing Neurons in the Median Preoptic Nucleus Modulate Heat-Dissipation Effectors in the Female Rat Endocrinology 2015 Mar 31 [PMID: 25825817] (IHC-Fr, Rat)
• True C, Nasrin Alam S, Cox K et al. Neurokinin B is critical for normal timing of sexual maturation but dispensable for adult reproductive function in female mice. Endocrinology 2015 Apr [PMID: 25574869] (Mouse)
• Cholanian M, Krajewski-Hall SJ, Levine RB et al. Electrophysiology of Arcuate Neurokinin B Neurons in Female Tac2-EGFP Transgenic Mice. Endocrinology 2014 Apr 15 [PMID: 24735328] (IHC-Fr, Mouse)
• Mittelman-Smith MA, Williams H, Krajewski-Hall SJ, Lai J, Ciofi P, McMullen NT, Rance NE. Arcuate Kisspeptin/Neurokinin B/Dynorphin (KNDy) Neurons Mediate the Estrogen Suppression of Gonadotropin Secretion and Body Weight. Endocrinology;153(6):2800-12. 2012 Jun. [PMID: 22508514] (IHC, Rat)
• Krajewski SJ, Burke MC, Anderson MJ et al. Forebrain projections of arcuate neurokinin B neurons demonstrated by anterograde tract-tracing and monosodium glutamate lesions in the rat Neuroscience 2010 Mar 17 [PMID: 20038444] (IHC, ICC/IF, Rat)
• Halasz J, Zelena D, Toth M et al. Substance P neurotransmission and violent aggression: the role of tachykinin NK(1) receptors in the hypothalamic attack area. Eur J Pharmacol. 2009 Jun 2 [PMID: 19344710] (IHC-Fr, Rat)
• Burke MC, Letts PA, Krajewski SJ, Rance NE. Coexpression of dynorphin and neurokinin B immunoreactivity in the rat hypothalamus: Morphologic evidence of interrelated function within the arcuate nucleus. J Comp Neurol;498(5):712-26. 2006 Oct 10. [PMID: 16917850]
• Krajewski SJ, Anderson MJ, Iles-Shih L Morphologic evidence that neurokinin B modulates gonadotropin-releasing hormone secretion via neurokinin 3 receptors in the rat median eminence. J Comp Neurol. 2005 Aug 29 [PMID: 16025449] (IHC-Fr, Rat)

Reviews for Neurokinin B Antibody (NB300-201) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Rat.

reviewed by: Andree Lessard IHC Rat 06/22/2012

Summary

• Application: Immunohistochemistry
• Sample Tested: rat tissue fixed with PFA and acrolein
• Species: Rat
• Comments: good labeling, minimal background, excellent specificity where a single band could be seen by western blot in cells transfected with the NK3 DNA; no bands in cells not transfected or transfected with vector alone...

Blocking

• Blocking Details: blcking agent 0.5% BSA-TS for 30 mins

Primary Anitbody

• Dilution Ratio: 1:1000, 12hr room temperature in 0.1% BSA-TS

Secondary Antibody

• Secondary Description: goat anti-rabbit biotin conjugated
• Secondary Manufacturer Cat#: Jacksons
• Secondary Concentration: 1:400

Details

• Detection Notes: DAb incubation 6 mins
• Fixation Details: PFA + acrolein

Comments

• Comments: good labeling, minimal background, excellent specificity where a single band could be seen by western blot in cells transfected with the NK3 DNA; no bands in cells not transfected or transfected with vector alone...

reviewed by: Christina Merkley IF Mouse 05/15/2012

Summary

• Application: Immunofluorescence
• Sample Tested: Mouse hypothalamus
• Species: Mouse
• Lot: A3
• Comments: Used in a dual- fluorescent protocol, we chose to amplify the NKB. The staining is strong and crisp in mouse tissue. For the amplification we used: Biotinylated goat anti- rabbit followed by ABC, Biotinylated Tyramide, and incubation in streptavidin (with rinses in PBS in between each step).

Blocking

• Blocking Details: 1% H202 solution (in PBS) for 10 min at RT, and also block in PBS + 0.04% Triton X-100 + 0.1% BSA for 1h at RT before primary.

Primary Anitbody

• Dilution Ratio: 1:5000 for 17 h at RT, diluted in PBS with 0.04% Triton X-100 and 1% BSA.

Secondary Antibody

• Secondary Description: Biotinylated goat anti- Rabbit (Vector Labs) in PBS with Triton X and BSA.
• Secondary Manufacturer Cat#: BA-1000
• Secondary Concentration: 1:500

Details

• Detection Notes: Detected using TSA Amplification, and stained using Cy5- streptavidin (1:100). Pos control: peptide preabsorption
• Fixation Details: 4% PFA Fixation
• Wash Description: PBS washes between each step. 4 x 5 minutes

Comments

• Comments: Used in a dual- fluorescent protocol, we chose to amplify the NKB. The staining is strong and crisp in mouse tissue. For the amplification we used: Biotinylated goat anti- rabbit followed by ABC, Biotinylated Tyramide, and incubation in streptavidin (with rinses in PBS in between each step).

FAQs for Neurokinin B Antibody (NB300-201). (Showing 1 - 1 of 1 FAQs).

1. Has NB300-201 been tested by IHC on frozen of paraffin embedded tissue section?
   • This antibody is suitable for use on both frozen and paraffin-embedded tissues.

Bioinformatics

• Gene Symbol: TAC3
• Entrez:
  • 6866(Human)
  • 21334(Mouse)
  • 29191(Rat)
• Uniprot:
  • P55099(Mouse)