Neurokinin B Antibody - BSA Free

Images

 
Immunohistochemistry: Neurokinin B Antibody [NB300-201] - Detection of Neurokinin B in rat brain tissue (Alexa 488 stain), using NB 300-201.
Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:200 dilution. The antibody depicted an ...read more
Immunohistochemistry-Paraffin: Neurokinin B Antibody [NB300-201] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse brain using Neurokinin B antibody at 1:100 dilution. The antibody showed a ...read more
Immunohistochemistry: Neurokinin B Antibody [NB300-201] - IF analysis of Neurokinin B in mouse hypothalamus. Image courtesy of an anonymous customer review.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Neurokinin B Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal sequence of mouse proNeurokinin B (~93% homology to the rat sequence). [UniProt# P55099]
Localization
Secreted
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TAC3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:500-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:100-1:2000
Application Notes
This Neurokinin B antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on paraffin-embedded and frozen sections. This antibody has been used on human hypothalamus and rat brain sections. In IHC, positive staining of the arcuate nucleus is seen. There has been customer feedback that this antibody works in Western blot; please note that this is a secreted protein.
Control
Neurokinin B Overexpression Lysate
Control Peptide
Neurokinin B Peptide (NB300-201PEP)
Reviewed Applications
Read 2 Reviews rated 4
using
NB300-201 in the following applications:

Publications
Read Publications using
NB300-201 in the following applications:

Reactivity Notes

Human, mouse and rat.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Neurokinin B Antibody - BSA Free

  • gamma tachykinin 3
  • neurokinin beta
  • neurokinin B-like
  • neuromedin K
  • preprotachykinin B
  • PRO1155
  • tachykinin 3
  • tachykinin-3
  • ZNEUROK1NKNBNKB

Background

Neurokinin B (NKB) belongs to tachykinin family of peptides that also includes substance P, NKA, neuropeptide K, neuropeptide-gamma, and hemokinin-1, and NKB is the only tachykinin synthesized from preprotachykinin-B gene (designated as TAC3 in mammals, except for rodents where it is named Tac2). Tachykinins are characterized by a common carboxyl-terminal amino acid sequence of FXGLM-NH2 , X being a hydrophobic residue and are active peptides which stimulates neurons, evoke behavioral responses, are potent vasodilators and secretagogues (secretion stimulators), and contract directly or indirectly smooth muscles. NKB is the cleaved product of neurokinin B-like protein Zneurok1 (also called preprotachykinin-B or tachykinin-3) and act as an endogenous agonist ligand for its receptor, the NK3 receptor (NKB receptor). NKB signaling has been reported to play critical role in pre-eclampsia which is a potentially life threatening disease and is essential to neuroprotection in neurodegenerative diseases as well as to normal reproductive development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Neurokinin B Antibody (NB300-201)(23)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-Fr, WB.


Filter By Application
ICC/IF
(1)
IHC
(6)
IHC-Fr
(7)
WB
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(5)
Rat
(12)
All Species
Showing Publications 1 - 10 of 23. Show All 23 Publications.
Publications using NB300-201 Applications Species
Senft RA, Freret ME, Sturrock N, Dymecki SM Neurochemically and hodologically distinct ascending VGLUT3 versus serotonin subsystems comprise the r2-Pet1 median raphe The Journal of neuroscience : the official journal of the Society for Neuroscience Feb 5 2021 [PMID: 33547164]
Liu, X, Yeo, S H Et al. Highly redundant neuropeptide volume co-transmission underlying episodic activation of the GnRH neuron dendron. Elife Jan 19 2021 [PMID: 33464205] (WB, Human) WB Human
Chen S, Gao XF, Zhou Y et al. A spinal neural circuitry for converting touch to itch sensation Nat Commun Oct 8 2020 [PMID: 33033265] (IHC-Fr, Mouse) IHC-Fr Mouse
Gao Z, Ma X, et al. Troxerutin protects against DHT-induced polycystic ovary syndrome in rats. J Ovarian Res 2020-09-13 [PMID: 32921318] (IHC, Rat) IHC Rat
Dees WL, Hiney JK, Srivastava VK Regulation of prepubertal dynorphin secretion in the medial basal hypothalamus of the female rat J. Neuroendocrinol. Dec 1 2019 [PMID: 31715027] (WB, Rat) WB Rat
Sakata D, Uruno T et al. Selective role of neurokinin B in IL-31-induced itch response in mice. J Allergy Clin Immunol Jan 10 2019 [PMID: 31405606] (IHC, Mouse) IHC Mouse
Fergani C, Leon S, Padilla SL et al. NKB signaling in the posterodorsal medial amygdala stimulates gonadotropin release in a kisspeptin-independent manner in female mice Elife Dec 19 2018 [PMID: 30565563] (IHC-Fr, Mouse) IHC-Fr Mouse
Srivastava Vinod K, Hiney Jill K, Dees William L et al. Alcohol Delays the Onset of Puberty in the Female Rat by Altering Key Hypothalamic Events. Alcoholism, Clinical and Experimental Research 2018 Jul [PMID: 29689132] (WB, Rat) WB Rat
Kanaya M, Iwata K, Ozawa H. Distinct dynorphin expression patterns with low- and high-dose estrogen treatment in the arcuate nucleus of female rats. Biol. Reprod. 2017 Oct 24 [PMID: 29069289] (Rat) Rat
Osuka S, Iwase A, Nakahara T et al. Kisspeptin in the hypothalamus of two rat models of polycystic ovary syndrome. Endocrinology. Dec 16 2016 [PMID: 27983870] (Rat) Rat
Show All 23 Publications.

Reviews for Neurokinin B Antibody (NB300-201) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Mouse, Rat.

Reviews using NB300-201:
Filter by Applications
IHC
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(1)
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry Neurokinin B NB300-201
Enlarge
4
reviewed by:
Andree Lessard
IHC Rat 06/22/2012
View

Summary

ApplicationImmunohistochemistry
Sample Testedrat tissue fixed with PFA and acrolein
SpeciesRat
Commentsgood labeling, minimal background, excellent specificity where a single band could be seen by western blot in cells transfected with the NK3 DNA; no bands in cells not transfected or transfected with vector alone...

Blocking

Blocking Detailsblcking agent 0.5% BSA-TS for 30 mins

Primary Anitbody

Dilution Ratio1:1000, 12hr room temperature in 0.1% BSA-TS

Secondary Antibody

Secondary Descriptiongoat anti-rabbit biotin conjugated
Secondary Manufacturer Cat#Jacksons
Secondary Concentration1:400

Details

Detection NotesDAb incubation 6 mins
Fixation DetailsPFA + acrolein

Comments

Commentsgood labeling, minimal background, excellent specificity where a single band could be seen by western blot in cells transfected with the NK3 DNA; no bands in cells not transfected or transfected with vector alone...
Immunofluorescence Neurokinin B NB300-201
Enlarge
4
reviewed by:
Christina Merkley
IF Mouse 05/15/2012
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse hypothalamus
SpeciesMouse
LotA3
CommentsUsed in a dual- fluorescent protocol, we chose to amplify the NKB. The staining is strong and crisp in mouse tissue. For the amplification we used: Biotinylated goat anti- rabbit followed by ABC, Biotinylated Tyramide, and incubation in streptavidin (with rinses in PBS in between each step).

Blocking

Blocking Details1% H202 solution (in PBS) for 10 min at RT, and also block in PBS + 0.04% Triton X-100 + 0.1% BSA for 1h at RT before primary.

Primary Anitbody

Dilution Ratio1:5000 for 17 h at RT, diluted in PBS with 0.04% Triton X-100 and 1% BSA.

Secondary Antibody

Secondary DescriptionBiotinylated goat anti- Rabbit (Vector Labs) in PBS with Triton X and BSA.
Secondary Manufacturer Cat#BA-1000
Secondary Concentration1:500

Details

Detection NotesDetected using TSA Amplification, and stained using Cy5- streptavidin (1:100). Pos control: peptide preabsorption
Fixation Details4% PFA Fixation
Wash DescriptionPBS washes between each step. 4 x 5 minutes

Comments

CommentsUsed in a dual- fluorescent protocol, we chose to amplify the NKB. The staining is strong and crisp in mouse tissue. For the amplification we used: Biotinylated goat anti- rabbit followed by ABC, Biotinylated Tyramide, and incubation in streptavidin (with rinses in PBS in between each step).

Product General Protocols

View specific protocols for Neurokinin B Antibody (NB300-201): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Neurokinin B Antibody (NB300-201). (Showing 1 - 1 of 1 FAQs).

  1. Has NB300-201 been tested by IHC on frozen of paraffin embedded tissue section?
    • This antibody is suitable for use on both frozen and paraffin-embedded tissues.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Andree Lessard
06/22/2012
Application: IHC
Species: Rat

Christina Merkley
05/15/2012
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol TAC3
Entrez
Uniprot