NK3R/TACR3/Neurokinin B Receptor Antibody

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Product Details

Summary
Reactivity Mu, Rt, GP, Sh, Hu(-)Species Glossary
Applications ICC/IF, IHC, IHC-Fr, IHC-FrFl, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NK3R/TACR3/Neurokinin B Receptor Antibody Summary

Immunogen
A synthetic peptide at the C-terminus of rat Neurokinin B Receptor, conjugated to bovine thyroglobulin. [UniProt# P16177]
Localization
In immunohistochemical experiments staining of the neuronal cell bodies and fibers is seen and staining is believed to be localized to the plasma membrane.
Specificity
NB300-102 is specific to the NK-3 receptor. Specificity was verified by the localization of immunohistochemical staining as well as pre-absorption studies with NK-3 peptide. (1)
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TACR3
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry Free-Floating
Application Notes
This Neurokinin B Receptor antibody is useful for Immunocytochemistry/Immunofluorescence and Immunohistochemistry on frozen sections.Use in Immunohistochemistry- free floating reported in scientific literature (PMID 25496429). It is not useful for Western blot.
Publications
Read Publications using
NB300-102 in the following applications:

Reactivity Notes

Rat, mouse and guinea pig in brain, spinal cord and ileum. Customer feedback suggests that human reactivity is negative. Sheep reactivity reported in scientific literature (PMID: 19912479).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for NK3R/TACR3/Neurokinin B Receptor Antibody

  • Neurokinin B Receptor
  • neurokinin beta receptor
  • Neuromedin-K Receptor
  • NK-3 receptor
  • NK3R
  • NK-3R
  • NK3RMGC148061
  • NKR
  • TAC3R
  • TAC3RL
  • tachykinin receptor 3MGC148060
  • TACR3

Background

Neurokinins belong to tachykinin family of signalling peptides and the best known human tachykinins are neurokinin B, (encoded by TAC3) and substance P (SP) as well as neurokinin A (NKA), (both encoded by the same TAC1 gene). Neurokinins are widely expressed in CNS, and exert effects on target cells through action at three closely related Gq-coupled neurokinin receptors: NK1R, NK2R, NK3R: encoded in humans by TACR1, TACR2 and TACR3 respectively. None of these neurokinin receptors are entirely specific for SP, NKA or NKB, however, they do show some degree of selectivity, and TACR3/NK3R (neurokinin B receptor or neuromedin-K receptor) behaves as cognate receptor for NKB in vivo. NK3R are predominantly expressed in neurons of PNS/CNS and in particular, in many of forebrain areas, such as frontal, parietal and cingulate cortices, and basal ganglia structures implicated in psychiatric disease states, and can modulate monoaminergic and amino acid neurotransmission within these structures. Neurokinin B and NK3R are also expressed in placenta, kidney, skeletal muscle and lung tissues, and had been implicated in diverse processes, including water homeostasis, pulmonary inflammation, cognition, and preeclampsia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for NK3R/TACR3/Neurokinin B Receptor Antibody (NB300-102)(27)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Guinea Pig, Sheep.

We have publications tested in 5 applications: ICC, ICC/IF, IHC, IHC-Fr, IHC-FrFl.


Filter By Application
ICC
(1)
ICC/IF
(4)
IHC
(5)
IHC-Fr
(3)
IHC-FrFl
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(3)
Rat
(9)
Guinea Pig
(1)
Sheep
(2)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using NB300-102 Applications Species
Hsu YW, Morton G, Guy EG et al. Dorsal Medial Habenula Regulation of Mood-Related Behaviors and Primary Reinforcement by Tachykinin-Expressing Habenula Neurons eNeuro 2016 Aug 02 [PMID: 27482535]
Ahn T, Fergani C, Coolen LM et al. Prenatal Testosterone Excess Decreases Neurokinin 3 Receptor Immunoreactivity within the Arcuate Nucleus KNDy cell population. J. Neuroendocrinol. 2014 Dec 10 [PMID: 25496429] (IHC-FrFl, Sheep)

Details:
NK3R antibody used for IHC-FrFl (IHC on free floating sections) application at 1 : 10,000 dilution in experiments involving control and prenatal testosterone-treated Suffolk ewes (see full text for detailed protocols and staining data).
IHC-FrFl Sheep
Nuhn JS, Fuerst PG. Developmental localization of adhesion and scaffolding proteins at the cone synapse. Gene Expr. Patterns. 2014 Aug 28 [PMID: 25176525]
Sawai N, Iijima N, Ozawa H, Matsuzaki T. Neurokinin B- and kisspeptin-positive fibers as well as tuberoinfundibular dopaminergic neurons directly innervate periventricular hypophyseal dopaminergic neurons in rats and mice. Neurosci. Res. 2014 May 09 [PMID: 24815059] (IHC-Fr, Rat) IHC-Fr Rat
de Andrade GB, Long SS, Fleming H et al. DSCAM localization and function at the mouse cone synapse. J Comp. Neurol. 2014 Jan 30 [PMID: 24477985] (IHC-Fr, Mouse)

Details:
Neurokinin B Receptor antibody used at 1:2000 dilution for IHC-Fr staining of type 1 and 2 cone bipolar cells in mice. Figure 2 A-D - adult wild-type and Dscam2J/2J retina section. Figure 14 O - sections of DscamFD/FD retina.
IHC-Fr Mouse
Hether S, Misono K, Lessard A. The neurokinin-3 receptor (NK3R) antagonist SB222200 prevents the apomorphine-evoked surface but not nuclear NK3R redistribution in dopaminergic neurons of the rat ventral tegmental area. Neuroscience 2013 May 11 [PMID: 23673279] (Rat) Rat
Nivison-Smith L, Sun D, Fletcher EL et al. Mapping kainate activation of inner neurons in the rat retina. J Comp Neurol 2013 Aug 1 [PMID: 23348566] (Rat) Rat
Sawai N, Iijima N, Takumi K et al. Immunofluorescent histochemical and ultrastructural studies on the innervation of kisspeptin/neurokinin B neurons to tuberoinfundibular dopaminergic neurons in the arcuate nucleus of rats Neurosci Res 2012 Jun 9 [PMID: 22691459] (IHC, ICC/IF, Rat) IHC, ICC/IF Rat
Misono K, Lessard A. Apomorphine-evoked redistribution of neurokinin-3 receptors in dopaminergic dendrites and neuronal nuclei of the rat ventral tegmental area. Neuroscience;203:27-38. 2012 Feb 17. [PMID: 22200547]
Krajewski SJ, Burke MC, Anderson MJ et al. Forebrain projections of arcuate neurokinin B neurons demonstrated by anterograde tract-tracing and monosodium glutamate lesions in the rat Neuroscience 2010 Mar 17 [PMID: 20038444]
Show All 27 Publications.

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Product General Protocols

View specific protocols for NK3R/TACR3/Neurokinin B Receptor Antibody (NB300-102): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for NK3R/TACR3/Neurokinin B Receptor Antibody (NB300-102). (Showing 1 - 1 of 1 FAQs).

  1. I was wondering about the Rabbit polyclonal antibody to Neurokinin-3-receptor, catalog #NB300-102. I am wondering about its information on the immunogen. You state that, NB300-102 was raised against a synthetic peptide at the c-terminus of rat Nk-3 conjugated to bovine thyroglobulin. I was wondering if you could tell me exactly which amino acids that this does correspond to? I am interested in comparing it to the Rabbit anti-Nk3R used for western blot, catalog#R-103-100, which states that it corresponds to the aa:434-452 of the absolute c-terminal of rat Nk3R protein.
    • The immunogen for this antibody is between amino acids 375 and 452 of rat NK3R. Any further information regarding the immunogen is considered proprietary.

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Bioinformatics

Gene Symbol TACR3
Entrez
Uniprot