Human Endostatin Quantikine ELISA Kit

• Reactivity: Hu
• Applications: ELISA
• Conjugate: HRP

Human Endostatin Quantikine ELISA Kit Summary

• Background: The Quantikine Human Endostatin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Endostatin in cell culture supernates, serum, plasma, and saliva. It contains E. coli-expressed recombinant human Endostatin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Endostatin showed linear curves that were parallel to... the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Endostatin.
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• Specificity: Natural and recombinant human Endostatin
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: COL18A1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human Endostatin Quantikine ELISA Kit

• antiangiogenic agent
• COL18A1
• collagen alpha-1(XVIII) chain
• collagen, type XVIII, alpha 1
• Endostatin
• FLJ27325
• FLJ34914
• human type XVIII collagen10endostatin
• KNO
• KNO1MGC74745
• Knobloch syndrome, type 1
• KS
• multi-functional protein MFP

Background

Endostatin is a 20 kDa proteolytic fragment of the C-terminal, non-collagenous (NC1) domain of type XVIII Collagen. It was originally identified as a factor produced by murine hemangioendothelioma cells that could specifically inhibit endothelial cell proliferation and angiogenesis. Although the molecular signals that trigger the release of Endostatin from type XVIII Collagen are not well understood, multiple proteases have been suggested to be involved in its generation including Cathepsins S, B, L, and V, Elastase, and matrix metalloproteinases (MMPs)-2, -7, and -9. Endostatin is of particular interest as it has been shown to inhibit the growth of many primary and metastatic tumors. It may also be involved in down-regulating angiogenesis during physiological processes such as wound healing and the establishment of placental circulation. The anti-angiogenic activity of Endostatin is attributable to its ability to inhibit endothelial cell proliferation and suppress VEGF-and FGF basic-induced endothelial cell migration and adhesion. Many of these effects are thought to be mediated by interactions between Endostatin and endothelial cell-expressed Transglutaminase 2, Heparin, and Integrins alpha 5 beta 1 and alpha V beta 3.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Endostatin (DNST0)(22)

Publications using DNST0

• FG Scurt, J Menne, S Brandt, A Bernhardt, PR Mertens, H Haller, C Chatzikyrk Systemic Inflammation Precedes Microalbuminuria in Diabetes Kidney Int Rep, 2019-06-21;4(10):1373-1386. 2019-06-21 [PMID: 31701047] (Human)
• YY Joo, JW Jang, SW Lee, SH Yoo, JH Kwon, SW Nam, SH Bae, JY Choi, SK Yoon Circulating pro- and anti-angiogenic factors in multi-stage liver disease and hepatocellular carcinoma progression Sci Rep, 2019-06-24;9(1):9137. 2019-06-24 [PMID: 31235729] (Human)
• J Zhang, D Nie, K Williamson, JL Rocha, MV Hogan, JH Wang Selectively activated PRP exerts differential effects on tendon stem/progenitor cells and tendon healing J Tissue Eng, 2019-01-16;10(0):2.04173E+15. 2019-01-16 [PMID: 30728936] (Human)
• M Holsti, A Wanhainen, C Lundin, M Björck, G Tegler, J Svensson, M Sund Circulating Vascular Basement Membrane Fragments are Associated with the Diameter of the Abdominal Aorta and Their Expression Pattern is Altered in AAA Tissue Eur J Vasc Endovasc Surg, 2018-04-12;0(0):. 2018-04-12 [PMID: 29656960] (Human)
• Borghini A, Manetti M, Nacci F, Bellando-Randone S, Guiducci S, Matucci-Cerinic M, Ibba-Manneschi L, Weber E Systemic Sclerosis Sera Impair Angiogenic Performance of Dermal Microvascular Endothelial Cells: Therapeutic Implications of Cyclophosphamide. PLoS ONE, 2015-06-15;10(6):e0130166. 2015-06-15 [PMID: 26076019] (Human)
• Marek-Trzonkowska N, Kwieczynska A, Reiwer-Gostomska M, Kolinski T, Molisz A, Siebert J Arterial Hypertension Is Characterized by Imbalance of Pro-Angiogenic versus Anti-Angiogenic Factors. PLoS ONE, 2015-05-07;10(5):e0126190. 2015-05-07 [PMID: 25951297] (Human)
• Hutzen , Brian, Bid , Hemant K, Houghton , Peter J, Pierson , Christop, Powell , Kimerly, Bratasz , Anna, Raffel , Corey, Studebaker , Adam W Treatment of medulloblastoma with oncolytic measles viruses expressing the angiogenesis inhibitors endostatin and angiostatin. BMC Cancer, 2014-03-19;14(0):206. 2014-03-19 [PMID: 24646176] (Human)
• Sponder M, Dangl D, Kampf S, Fritzer-Szekeres M, Strametz-Juranek J Exercise increases serum endostatin levels in female and male patients with diabetes and controls. Cardiovasc Diabetol, 2014-01-06;13(0):6. 2014-01-06 [PMID: 24393402] (Human)
• Battinelli , Elisabet, Markens , Beth A, Kulenthirarajan , Rajesh A, Machlus , Kellie R, Flaumenhaft , Robert, Italiano , Joseph E Anticoagulation inhibits tumor cell-mediated release of platelet angiogenic proteins and diminishes platelet angiogenic response. Blood, 2013-09-24;123(1):101-12. 2013-09-24 [PMID: 24065244] (Human)
• Leifler K, Svensson S, Abrahamsson A, Bendrik C, Robertson J, Gauldie J, Olsson A, Dabrosin C Inflammation induced by MMP-9 enhances tumor regression of experimental breast cancer. J Immunol, 2013-03-15;190(8):4420-30. 2013-03-15 [PMID: 23509357] (Mouse)
• Zheng , Lan, Zhang , Dongmei, Chen , Xianchen, Yang , Li, Wei , Yuquan, Zhao , Xia Antitumor activities of human placenta-derived mesenchymal stem cells expressing endostatin on ovarian cancer. PLoS ONE, 2012-07-24;7(7):e39119. 2012-07-24 [PMID: 22911684] (Human)
• Landt S, Mordelt K, Schwidde I, Barinoff J, Korlach S, Stoblen F, Lichtenegger W, Sehouli J, Kummel S Prognostic significance of the angiogenic factors angiogenin, endoglin and endostatin in cervical cancer. Anticancer Res., 2011-08-01;31(8):2651-5. 2011-08-01 [PMID: 21778318] (Human)
• Landt S, Heidecke H, Korlach S, Reuter C, Schwidde I, Barinoff J, Thill M, Sehouli J, Kummel S In vitro vascular tube formation testing as a tool for treatment individualisation in patients with cervical cancer. Anticancer Res., 2011-08-01;31(8):2609-15. 2011-08-01 [PMID: 21778312] (Human)
• Sun XD, Jeng L, Bolliet C, Olsen BR, Spector M Non-viral endostatin plasmid transfection of mesenchymal stem cells via collagen scaffolds. Biomaterials, 2008-12-06;30(6):1222-31. 2008-12-06 [PMID: 19059640] (Goat)
• Mellon MJ, Bae KH, Steding CE, Jimenez JA, Kao C, Gardner TA Suppression of renal cell carcinoma growth and metastasis with sustained antiangiogenic gene therapy. Hum. Gene Ther., 2008-05-01;19(5):487-95. 2008-05-01 [PMID: 18507514] (Human)
• Holmes CE, Huang JC, Pace TR, Howard AB, Muss HB Tamoxifen and aromatase inhibitors differentially affect vascular endothelial growth factor and endostatin levels in women with breast cancer. Clin. Cancer Res., 2008-05-15;14(10):3070-6. 2008-05-15 [PMID: 18483373] (Human)
• Bendrik C, Robertson J, Gauldie J, Dabrosin C Gene transfer of matrix metalloproteinase-9 induces tumor regression of breast cancer in vivo. Cancer Res., 2008-05-01;68(9):3405-12. 2008-05-01 [PMID: 18451168] (Human)
• Ohlund D, Ardnor B, Oman M, Naredi P, Sund M Expression pattern and circulating levels of endostatin in patients with pancreas cancer. Int. J. Cancer, 2008-06-15;122(12):2805-10. 2008-06-15 [PMID: 18360823] (Human)
• Li HL, Li S, Shao JY, Lin XB, Cao Y, Jiang WQ, Liu RY, Zhao P, Zhu XF, Zeng MS, Guan ZZ, Huang W Pharmacokinetic and pharmacodynamic study of intratumoral injection of an adenovirus encoding endostatin in patients with advanced tumors. Gene Ther., 2007-12-20;15(4):247-56. 2007-12-20 [PMID: 18097470] (Human)
• Perryman LA, Blair JM, Kingsley EA, Szymanska B, Ow KT, Wen VW, MacKenzie KL, Vermeulen PB, Jackson P, Russell PJ Over-expression of p53 mutants in LNCaP cells alters tumor growth and angiogenesis in vivo. Biochem. Biophys. Res. Commun., 2006-05-11;345(3):1207-14. 2006-05-11 [PMID: 16723121] (Human)
• Ferrandina G, Ranelletti FO, Legge F, Salutari V, Martinelli E, Fattorossi A, Lorusso D, Zannoni G, Vellone V, Paglia A, Scambia G Celecoxib up-regulates the expression of the zeta chain of T cell receptor complex in tumor-infiltrating lymphocytes in human cervical cancer. Clin. Cancer Res., 2006-04-01;12(7):2055-60. 2006-04-01 [PMID: 16609015] (Human)
• Furstenberger G, von Moos R, Lucas R, Thurlimann B, Senn HJ, Hamacher J, Boneberg EM Circulating endothelial cells and angiogenic serum factors during neoadjuvant chemotherapy of primary breast cancer. Br. J. Cancer, 2006-02-27;94(4):524-31. 2006-02-27 [PMID: 16450002] (Human)

FAQs for Endostatin (DNST0). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: COL18A1