Integrin alpha 2b/CD41 Antibody (M148) - BSA Free

• Reactivity: Hu
• Applications: Flow, IHC, IP, CyTOF-ready, ICC/IF
• Clone: M148
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Integrin alpha 2b/CD41 Antibody (M148) - BSA Free Summary

• Description: Novus Biologicals Mouse Integrin alpha 2b/CD41 Antibody (M148) - BSA Free (NB100-2614) is a monoclonal antibody validated for use in IHC, Flow, ICC/IF and IP. Anti-Integrin alpha 2b/CD41 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Homogenized human medulloblastoma tissue.
• Localization: Cell Membrane
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA2B
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10 - 1:1000
  • Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
• Application Notes: No binding is observed to marrow constituents other than platelets and megakaryocytes. This antibody is CyTOF ready. Positive control(s): human platelets and megakaryocytes.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Integrin alpha 2b/CD41 Antibody (M148) - BSA Free

• CD41 antigen
• CD41
• CD41BHPA3
• GP2B
• GP2Bintegrin alpha-IIb
• GPalpha IIb
• GPIIb
• GTA
• HPA3
• Integrin alpha 2b
• integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigenCD41)
• integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigenCD41B)
• ITGA2b
• ITGAB
• platelet fibrinogen receptor, alpha subunit
• Platelet membrane glycoprotein IIb
• platelet-specific antigen BAK

Background

Integrin alpha 2b (CD41) is a calcium-dependent, noncovalently associated heterodimer and contains a heavy chain (GPIIb alpha) and a light chain (GPIIb beta) linked by a single disulfide bond. The Integrin alpha 2B chain interacts with the Integrin beta 3 subunit/CD61 to form the platelet glycoprotein complex, gpIIb/IIIa. It is expressed on platelets and megakaryocytes. Ligands for the gpIIb/IIIa heterodimer include fibrinogen, von Willebrand factor, fibronectin, vitronectin, and thrombospondin. The gpIIb/IIIa complex is the major Integrin on platelets and is important for platelet adhesion and aggregation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 2b/CD41 Antibody (NB100-2614)(4)

Publications using NB100-2614

• Thomas KA, Rassam RMG, Kar R et al. Trauma patients have reduced ex vivo flow-dependent platelet hemostatic capacity in a microfluidic model of vessel injury. PloS one 2024-07-10 [PMID: 38985805]
• Shea SM, Thomas KA, Rassam RMG Et al. Dose-Dependent Von Willebrand Factor Inhibition by Aptamer BB-031 Correlates with Thrombolysis in a Microfluidic Model of Arterial Occlusion Pharmaceuticals (Basel) 2022-12-23 [PMID: 36558901] (ICC/IF, Human)
  
  Details:
  Citation using the Janelia Fluor 646 version of this antibody.
• Golomingi M, Kohler J, Jenny L et al. Complement lectin pathway components MBL and MASP-1 promote haemostasis upon vessel injury in a microvascular bleeding model Frontiers in immunology 2022-08-12 [PMID: 36032172] (Human)
  
  Details:
  Microfluidics
• Younes Zaid, Florian Puhm, Isabelle Allaeys, Abdallah Naya, Mounia Oudghiri, Loubna Khalki, Youness Limami, Nabil Zaid, Khalid Sadki, Rafiqua Ben El Haj, Wissal Mahir, Lamiae Belayachi, Bouchra Belefquih, Amina Benouda, Amine Cheikh, Marc-André Langlois, Yahia Cherrah, Louis Flamand, Fadila Guessous, Eric Boilard Platelets Can Associate With SARS-CoV-2 RNA and Are Hyperactivated in COVID-19 Circulation Research 2020-09-17 [PMID: 32938299]

Bioinformatics

• Gene Symbol: ITGA2B
• Entrez:
  • 3674(Human)
  • 16399(Mouse)
  • 29350(Rat)