CDC25B Antibody [Unconjugated]

Images

 
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, DA3 mouse myeloma cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 0.3 µg/mL of Goat Anti-Human/Mouse/Rat ...read more
CDC25B was detected in immersion fixed HL‑60 human acute promyelocytic leukemia cell line using Goat Anti-Human/Mouse/Rat CDC25B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1649) at 15 µg/mL for 3 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

CDC25B Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CDC25B
Glu391-Gln580
Accession # P30305
Specificity
Detects human, mouse, and rat CDC25B. Four splice variants of CDC25B, with molecular weights of 61, 63, 65, and 67 kDa, are known. The immunogen selected for this antibody is common to all variants. Immunoreactivity consistent with at least 3 variants has been detected in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) CDC25A or rhCDC25C.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CDC25B
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 10-25 ug/mL
  • Western Blot 0.3 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CDC25B Antibody [Unconjugated]

  • CDC25B
  • CDC25HU2
  • cell division cycle 25 homolog B (S. cerevisiae)
  • cell division cycle 25 homolog B (S. pombe)
  • cell division cycle 25B
  • Dual specificity phosphatase Cdc25B
  • EC 3.1.3.48
  • M-phase inducer phosphatase 2

Background

Cell Division Cycle 25B (Cdc25B) phosphatase removes inorganic phosphate groups covalently attached to tyrosine, serine and threonine residues in proteins (1). Breast cancer patients bearing tumors containing high levels of Cdc25B have been found to have a greater incidence of aggressive, high-grade tumors than those with low Cdc25B levels (2). In cells, the levels of Cdc25B activity are highest during the G2/M transition of the cell cycle, where it is suspected to be involved in “checkpoint” control of cell cycle progression (3). Overexpression of Cdc25B reduces the G2/M cell cycle block caused by ionizing radiation (4). Although activated by phosphorylation, Ser323 phosphorylation causes the enzyme to bind the protein 14-3-3, preventing substrate access to the catalytic site (5). One of the major substrates of Cdc25B is Cdc2, a kinase that is activated by dephosphorylation (6). The recombinant protein is truncated to remove the N‑terminal regulatory domains and is fully active.

  1. Draetta, G. and J. Eckstein (1997) Biochim. Biophys. Acta 1332:M53.
  2. Galaktionov, K. et al. (1995) Science 269:1575.
  3. Lammer, C. et al. (1998) J. Cell Sci. 111:2445.
  4. Miyata, H. et al. (2001) Cancer Res. 61:3188.
  5. Forrest, A. and B. Gabrielli (2001) Oncogene 20:4393.
  6. Gautier, J. et al. (1991) Cell 67:197.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CDC25B
Uniprot