CDC25C Antibody (461908) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, CHP-100 human neuroblastoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse/Rat CDC25C Monoclonal Antibody (Catalog # MAB4459) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CDC25C at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 461908
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

CDC25C Antibody (461908) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human CDC25C
  Ser2-Pro473
  Accession # P30307
• Specificity: Detects human, mouse and rat CDC25C in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) CDC25A or rhCDC25B is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: CDC25C
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CDC25C Antibody (461908) [Unconjugated]

• CDC 25
• CDC25
• CDC25C
• cell division cycle 25 homolog C (S. cerevisiae)
• cell division cycle 25 homolog C (S. pombe)
• cell division cycle 25C
• Dual specificity phosphatase Cdc25C
• EC 3.1.3.48
• mitosis inducer CDC25
• M-phase inducer phosphatase 3
• phosphotyrosine phosphatase

Background

Cell Division Cycle 25C (CDC25C) is a dual serine/threonine and tyrosine phosphatase. There are four splice variants with molecular weights of 46, 47, 49, and 53 kDa. The smaller variants lack key regulatory sites and their upregulation has been observed in prostate cancer. CDC25C regulates the G2/M cell cycle transition by dephosphorylating proteins such as Chk1. Its cellular distribution is controlled by phosphorylations that enhance 14-3-3 binding, block nuclear localization, and inhibit enzymatic activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDC25C Antibody (MAB4459)(2)

Publications using MAB4459

• Liqing Wei, Li Li, Li Liu, Ru Yu, Xing Li, Zhenzhao Luo Knockdown of Annexin-A1 Inhibits Growth, Migration and Invasion of Glioma Cells by Suppressing the PI3K/Akt Signaling Pathway ASN Neuro 3/12/2021 [PMID: 33706561]
• Mani M, Carrasco DE, Zhang Y, Takada K, Gatt ME, Dutta-Simmons J, Ikeda H, Diaz-Griffero F, Pena-Cruz V, Bertagnolli M, Myeroff LL, Markowitz SD, Anderson KC, Carrasco DR BCL9 promotes tumor progression by conferring enhanced proliferative, metastatic, and angiogenic properties to cancer cells. Cancer Res., 2009-09-08;69(19):7577-86. 2009-09-08 [PMID: 19738061] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: CDC25C
• Uniprot:
  • P30307()