CDC25A Antibody (336445)

Western blot shows lysates of MCF-7 human breast cancer cell line, CTLL-2 mouse cytotoxic T cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CDC25A Monoclonal Antibody (Catalog # MAB1648) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for CDC25A at approximately 55-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 336445
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

CDC25A Antibody (336445) Summary

• Immunogen: E. coli-derived recombinant human CDC25A
  Glu2-Leu523
  Accession # AAA58415
• Specificity: Detects human, mouse, and rat CDC25A in direct ELISAs and Western blots. In direct ELISA and Western blots, this antibody does not cross-react with recombinant human (rh) CDC25B or rhCDC25C.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDC25A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CDC25A Antibody (336445)

• CDC25A
• CDC25A2
• CDC25A2-CAG isoform
• cell division cycle 25 homolog A (S. cerevisiae)
• cell division cycle 25 homolog A (S. pombe)
• cell division cycle 25A
• Dual specificity phosphatase Cdc25A
• EC 3.1.3.48
• M-phase inducer phosphatase 1

Background

Cell Division Cycle 25A (CDC25A) phosphatase removes inorganic phosphate groups covalently attached to tyrosine, serine and threonine residues in proteins (1). Overexpression of CDC25A in small mammary carcinomas has been associated with a poor patient survival prognosis (2). Levels of CDC25A activity are highest in S phase of the cell cycle, where it is suspected to be involved in “checkpoint” control of cell cycle progression (3). Induction of DNA damage with ultraviolet or ionizing radiation causes an increase in CDC25A ubiquinylation and proteosomal degradation, leading to cell cycle block between G1 and S phases (4). One of its major substrates, the kinase CDC2, is activated by dephosphorylation (5). Recombinant CDC25A is truncated to remove the N-terminal regulatory domains and is fully active.

1. Draetta, G. and J. Eckstein (1997) Biochim. Biophys. Acta. 1332:M53.
2. Cangi, M. et al. (2000) J. Clin. Invest. 106:753.
3. Hoffmann, I. et al. (1995) EMBO J. 13:4302.
4. Sagata, N. (2002) Science 298:1905.
5. Gautier, J. et al. (1991) Cell 67:197.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CDC25A Antibody (MAB1648)(2)

Publications using MAB1648

• X Wang, Z Chen, AK Mishra, A Silva, W Ren, Z Pan, JH Wang Chemotherapy-induced differential cell cycle arrest in B cell lymphomas affects their sensitivity to Wee1 inhibition Haematologica, 2017-12-07;0(0):. 2017-12-07 [PMID: 29217775] (Western Blot, Mouse)
• Dutta-Simmons J, Zhang Y, Gorgun G, Gatt M, Mani M, Hideshima T, Takada K, Carlson NE, Carrasco DE, Tai YT, Raje N, Letai AG, Anderson KC, Carrasco DR Aurora kinase A is a target of Wnt/beta-catenin involved in multiple myeloma disease progression. Blood, 2009-08-03;114(13):2699-708. 2009-08-03 [PMID: 19652203] (Western Blot, Human)

Bioinformatics

• Gene Symbol: CDC25A
• Uniprot:
  • AAA58415()