CDC25A Antibody (336445)

Images

 
Western blot shows lysates of MCF‑7 human breast cancer cell line, CTLL‑2 mouse cytotoxic T cell line, and Nb2‑11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat CDC25A Monoclonal ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
336445
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

CDC25A Antibody (336445) Summary

Immunogen
E. coli-derived recombinant human CDC25A
Glu2-Leu523
Accession # AAA58415
Specificity
Detects human, mouse, and rat CDC25A in direct ELISAs and Western blots. In direct ELISA and Western blots, this antibody does not cross-react with recombinant human (rh) CDC25B or rhCDC25C.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CDC25A
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL
Publications
Read Publications using
MAB1648 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CDC25A Antibody (336445)

  • CDC25A
  • CDC25A2
  • CDC25A2-CAG isoform
  • cell division cycle 25 homolog A (S. cerevisiae)
  • cell division cycle 25 homolog A (S. pombe)
  • cell division cycle 25A
  • Dual specificity phosphatase Cdc25A
  • EC 3.1.3.48
  • M-phase inducer phosphatase 1

Background

Cell Division Cycle 25A (CDC25A) phosphatase removes inorganic phosphate groups covalently attached to tyrosine, serine and threonine residues in proteins (1). Overexpression of CDC25A in small mammary carcinomas has been associated with a poor patient survival prognosis (2). Levels of CDC25A activity are highest in S phase of the cell cycle, where it is suspected to be involved in “checkpoint” control of cell cycle progression (3). Induction of DNA damage with ultraviolet or ionizing radiation causes an increase in CDC25A ubiquinylation and proteosomal degradation, leading to cell cycle block between G1 and S phases (4). One of its major substrates, the kinase CDC2, is activated by dephosphorylation (5). Recombinant CDC25A is truncated to remove the N-terminal regulatory domains and is fully active.

  1. Draetta, G. and J. Eckstein (1997) Biochim. Biophys. Acta. 1332:M53.
  2. Cangi, M. et al. (2000) J. Clin. Invest. 106:753.
  3. Hoffmann, I. et al. (1995) EMBO J. 13:4302.
  4. Sagata, N. (2002) Science 298:1905.
  5. Gautier, J. et al. (1991) Cell 67:197.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CDC25A Antibody (MAB1648)(2)

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Bioinformatics

Gene Symbol CDC25A
Entrez
Uniprot