CD98 Antibody

Immunohistochemistry-Paraffin: CD98 Antibody [NBP1-32148] - Paraffin-embedded SCC15 xenograft, using antibody at 1:100 dilution.

Western Blot: CD98 Antibody [NBP1-32148] - SLC3A2 antibody detects SLC3A2 protein by Western blot analysis. A431 whole cell extracts, A431 cytosol and A431 membrane extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SLC3A2 antibody (NBP1-32148) diluted at 1:500.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CD98 Antibody Summary

• Description: Novus Biologicals Rabbit CD98 Antibody (NBP1-32148) is a polyclonal antibody validated for use in IHC and WB. Anti-CD98 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human CD98. The exact sequence is proprietary.
• Localization: Apical cell membrane, Melanosome
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SLC3A2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rabbit (83%), Bovine (81%), Canine (81%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for CD98 Antibody

• 4F2
• 4F2hc
• 4T2HC
• antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43,4F2HC
• CD98 antigen
• CD98 heavy chain
• CD98
• CD98HC
• CH98hc
• FRP-1
• heavy chain
• Lymphocyte activation antigen 4F2 large subunit
• MDU1antigen defined by monoclonal 4F2, heavy chain
• monoclonal 44D7
• NACAE4F2 cell-surface antigen heavy chain
• SLC3A2
• solute carrier family 3 (activators of dibasic and neutral amino acidtransport), member 2,4F2 heavy chain antigen

Background

This gene is a member of the solute carrier family and encodes a cell surface, transmembrane protein with an alpha amylase domain. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. It associates with integrins and mediates integrin-dependent signaling related to normal cell growth and tumorigenesis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for CD98 Antibody (NBP1-32148)(1)

Publication using NBP1-32148

• Lee D, Kim HS, Kim HU et al. Expression profile of CD98 heavy chain and L-type amino acid transporter 1 and its prognostic significance in colorectal cancer Pathology, research and practice 2021-12-02 [PMID: 34942513] (IF/IHC, Human)

Bioinformatics

• Gene Symbol: SLC3A2
• Entrez:
  • 6520(Human)
• Uniprot:
  • P08195()