Chimaerin 2 Antibody

Western Blot: Chimaerin 2 Antibody [NBP3-12294] - 1:1000 antibody dilution in DiluObuffer. Apparent MW is 50 KDa.

Immunohistochemistry-Paraffin: Chimaerin 2 Antibody [NBP3-12294] - Rat cerebellum. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC, IHC-P, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Chimaerin 2 Antibody Summary

• Immunogen: Synthetic peptide taken within amino acid region 150-180 on beta 2-chimaerin protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHN2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:200
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Chimaerin 2 Antibody

• ARHGAP3beta chimerin
• BCH
• beta-chimaerin
• beta-chimerin
• chimerin (chimaerin) 2
• MGC138360
• Rho GTPase-activating protein 3
• RHOGAP3
• rho-GTPase-activating protein 3

Background

Chimaerin 2 is a protein expressed in the brain and pancreas with two isoforms, measuring 468 and 332 amino acids in length with weights of approximately 54 and 38 kDa respectively. Chimaerin 2 functions as a GTP-ase activating protein for p21-rac, and a deficiency in this protein could lead to tumor progression. Current studies are being done on several diseases and disorders related to this protein including b-cell lymphomas, schizophrenia, sleeping sickness, insulin resistance, diabetic retinopathy, malignant glioma, nephropathy, spasticity, astrocytoma, and breast cancer. Chimaerin 2 has also been shown to have interactions with BAG6, NCK1, RAC1, ERBB3, and EIF3C in pathways such as the Rho GTPase cycle, signal transduction, and regulations of RAC1 activity pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CHN2