Home » TTF-1 / NKX2-1 » TTF-1 / NKX2-1 Antibodies » TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690)

TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690)

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Immunohistochemistry-Paraffin: TTF1 / NKX2.1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - Human Lung Carcinoma stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/690)
Immunohistochemistry-Paraffin: TTF1 / NKX2.1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - Human Lung Carcinoma stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/690)
Immunohistochemistry-Paraffin: TTF1 / NKX2.1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - Human Thyroid stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/690)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Flow, IHC-P, ICC/IF
Clone
8G7G3/1 + NX2.1/690
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP2-47773AF405
Alexa Fluor 488
NBP2-47773AF488
Alexa Fluor 647
NBP2-47773AF647
Alexa Fluor 700
NBP2-47773AF700
Allophycocyanin
NBP2-47773APC
Biotin
NBP2-47773B
DyLight 350
NBP2-47773UV
DyLight 405
NBP2-47773V
DyLight 488
NBP2-47773G
DyLight 550
NBP2-47773R
DyLight 650
NBP2-47773C
DyLight 680
NBP2-47773FR
DyLight 755
NBP2-47773IR
FITC
NBP2-47773F
HRP
NBP2-47773H
Janelia Fluor 549
NBP2-47773JF549
Janelia Fluor 646
NBP2-47773JF646
PE
NBP2-47773PE
PerCP
NBP2-47773PCP
View Available Formulations
Catalog# & Formulation Size Price
IHC-Prediluted
NBP2-48118
Azide and BSA Free
NBP2-47773-0.1mg
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TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) Summary

Immunogen
Rat full length TTF-1 recombinant protein (8G7G3/1); Recombinant TTF-1 protein (NX2.1/690)
Localization
Nuclear
Marker
Thyroid & Lung Epithelial Marker
Specificity
Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates.Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
NKX2-1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Hu-chromosome location: 14q13.3
Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-44501 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690)

  • BCH
  • benign chorea
  • BHC
  • Homeobox protein NK-2 homolog A
  • homeobox protein Nkx-2.1
  • NK2 homeobox 1
  • NK-2 homolog A
  • NKX2.1
  • NKX2-1
  • NKX2A
  • NKX2ANKX2.1
  • T/EBP
  • Thyroid nuclear factor 1
  • Thyroid transcription factor 1BHC
  • TITF1
  • TITF1TEBP
  • TTF1
  • TTF-1
  • TTF1BCH
  • TTF-1NK-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody [NBP1-88080] - Staining in human placenta and skeletal muscle tissues. Corresponding KRT7 RNA-seq data are presented for the same tissues.
Western Blot: Cytokeratin 7 Antibody [NBP1-88080] - Analysis in human cell line SiHa.
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
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Immunohistochemistry-Paraffin: SP-B/Surfactant Protein B Antibody [NBP2-33982] - Staining in human lung and kidney tissues using anti-SFTPB antibody. Corresponding SFTPB RNA-seq data are presented for the same tissues.
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Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody [NBP1-80670] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody [NBP1-80670] - Staining in human thyroid gland and cerebral cortex tissues using anti-TPO antibody. Corresponding TPO RNA-seq data are presented for the same tissues.
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Prosurfactant
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NBP1-87201
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Publications for TTF-1 / NKX2-1 Antibody (NBP2-44501) (0)

There are no publications for TTF-1 / NKX2-1 Antibody (NBP2-44501).
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Review for TTF-1 / NKX2-1 Antibody (NBP2-44501) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-44501:
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Immunocytochemistry TTF-1 / NKX2-1 NBP2-44501
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reviewed by:
Feng Li
 ICC Mouse 05/30/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedThyroid tissue
SpeciesMouse
Lot7080-3p170314

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PTMs for TTF-1 / NKX2-1 Antibody (NBP2-44501)

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Immunocytochemistry TTF-1 / NKX2-1 NBP2-44501
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 Feng Li
05/30/2017
Application: ICC
Species: Mouse
read this review

Bioinformatics

Gene Symbol NKX2-1
Uniprot