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TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690)

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Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - Human Lung Carcinoma stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/690)
Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - Human Lung Carcinoma stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/690)
Immunohistochemistry: TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - TTF1 staining in thyroid tissue of wild-type C57 mouse (red). Dilution is 1:100. Image from a verified customer review.
Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - Human Thyroid stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/690)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P, ICC/IF
Clone
8G7G3/1 + NX2.1/690
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47773AF350
Alexa Fluor 405
NBP2-47773AF405
Alexa Fluor 488
NBP2-47773AF488
Alexa Fluor 532
NBP2-47773AF532
Alexa Fluor 594
NBP2-47773AF594
Alexa Fluor 647
NBP2-47773AF647
Alexa Fluor 700
NBP2-47773AF700
Alexa Fluor 750
NBP2-47773AF750
Allophycocyanin
NBP2-47773APC
Biotin
NBP2-47773B
DyLight 350
NBP2-47773UV
DyLight 405
NBP2-47773V
DyLight 488
NBP2-47773G
DyLight 550
NBP2-47773R
DyLight 594
NBP2-47773DL594
DyLight 650
NBP2-47773C
DyLight 680
NBP2-47773FR
DyLight 755
NBP2-47773IR
FITC
NBP2-47773F
HRP
NBP2-47773H
Janelia Fluor 549
NBP2-47773JF549
Janelia Fluor 646
NBP2-47773JF646
PE
NBP2-47773PE
PerCP
NBP2-47773PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-47773-0.1mg
IHC-Prediluted
NBP2-48118
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TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) Summary

Immunogen
Rat full length TTF-1 / NKX2-1 recombinant protein (8G7G3/1); Recombinant TTF-1 / NKX2-1 protein (NX2.1/690) (Uniprot: P43699)
Localization
Nuclear
Marker
Thyroid & Lung Epithelial Marker
Isotype
IgG1 Kappa/IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NKX2-1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-44501 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47773)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690)

  • BCH
  • benign chorea
  • BHC
  • Homeobox protein NK-2 homolog A
  • homeobox protein Nkx-2.1
  • NK2 homeobox 1
  • NK-2 homolog A
  • NKX2.1
  • NKX2-1
  • NKX2A
  • NKX2ANKX2.1
  • T/EBP
  • Thyroid nuclear factor 1
  • Thyroid transcription factor 1BHC
  • TITF1
  • TITF1TEBP
  • TTF1
  • TTF-1
  • TTF1BCH
  • TTF-1NK-2

Background

The thyroid transcription factor 1 (TTF-1) is a homeodomain-containing transcription factor that activates the transcriptional activity of thyroid-specific gene promoters by binding to them. Hence, TTF-1 is crucial in the maintenance of the thyroid differentiation phenotype (1). Thyroid dysgenesis (TD) is responsible for most cases of congenital hypothyroidism, a condition that affects about one in 4000 newborns. Mutations in PAX8, TTF1, or FOXE1 may account for congenital hypothyroidism in patients with either isolated TD or TD with associated malformations involving kidney, lung, forebrain, and palate (2). In addition to its presence in thyroid gland epithelium, the human TTF-1 protein was detected by immunohistochemistry in human fetal lung as early as 11 weeks of gestation, being localized in the nuclei of epithelial cells of the developing airways. After birth, TTF-1 was selectively expressed in Type II epithelial cells in the alveoli and in subsets of bronchiolar epithelial cells in the conducting regions of the lung (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).
Immunohistochemistry: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Imaging of Mouse stomach tissue. Positive staining can only be observed inside the thyroid cells and the follicle in thyroid tissue. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). Dilution is 1:100. IHC-P image submitted by a verified customer review.
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(1 Publication)
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Flow Cytometry: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Analysis of CRC cells with Cytokeratin 20 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Staining Cytokeratin 20 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Analysis of paraffin-embedded human colon cancer tissue using anti-Cytokeratin 20 antibody. Counter stained with hematoxylin.
Species: Hu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
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Antibody (266815)
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Substance P was detected in immersion fixed paraffin-embedded sections of human spinal cord using 8 µg/mL Human/Mouse/Rat Substance P Monoclonal Antibody (Catalog # MAB4375) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the spinal cord dorsal horn. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Cytokeratin 7
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Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Lane view shows a specific band for Cytokeratin 7 at 5 ug/mL in HeLa cell lysate.Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 61 kDa. Image from an internal validation.
Flow Cytometry: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Flow Cytometric Analysis of HeLa cells using Cytokeratin 7 Antibody (OV-TL12/30) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Simple Western: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 7 antibody was used at 1 ug/mL dilution of HeLa lysates(s) respectively.
(1 Review)
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Applications: CyTOF-ready, Flow, ICC/IF, IF, IHC, IHC-P, Simple Western, WB
SP-B/Surfactant
Protein B Antibody (R ...
NBP2-77431
Western Blot: SP-B/Surfactant Protein B Antibody (RM370) [NBP2-77431] - Western Blot of human lung tissue lysate using NBP2-77431.
Immunohistochemistry: SP-B/Surfactant Protein B Antibody (RM370) [NBP2-77431] - Immunohistochemical staining of formalin fixed and paraffin embedded human lung cancer tissue section using NBP2-77431.
Species: Hu
Applications: IHC, WB
Thyroid Peroxidase
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NBP2-27544
Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody [NBP2-27544] - Staining of Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: Thyroid Peroxidase Antibody [NBP2-27544] - (0.1 ug/ml) Analysis of Thyroid Gland lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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PAX8 Antibody
NBP1-32440
Western Blot: PAX8 Antibody [NBP1-32440] - 293T whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - Rat thyroid gland. PAX8 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunocytochemistry/Immunofluorescence: PAX8 Antibody [NBP1-32440] - Stepwise differentiation of human iPSCs towards renal progenitor cells (RPCs). Schematic description of the two-stage protocol applied to iPSC-renal commitment. Immunofluorescence of iPSCs (derived from retroviral transfected dermal fibroblasts) exposed to differentiating media. Pictured are the IM and MM marker expressions as Wt1, Pax8, Pax2, Six2 and Sall1. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep08826), licensed under a CC-BY licence.
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Prosurfactant
Protein C Antibody
NBP1-87201
Western Blot: Prosurfactant Protein C Antibody [NBP1-87201] - Analysis in human lung tissue.
Immunohistochemistry-Paraffin: Prosurfactant Protein C Antibody [NBP1-87201] - Staining of human kidney shows no positivity in cells in glomeruli as expected.
Immunohistochemistry-Paraffin: Prosurfactant Protein C Antibody [NBP1-87201] - Staining of human lung shows moderate to strong cytoplasmic positivity in respiratory epithelial cells.
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Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571] - Staining in human cervix, uterine and skeletal muscle tissues using anti-CKAP4 antibody. Corresponding CKAP4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571] - Staining of human bone marrow, cerebral cortex, cervix, uterine and skeletal muscle using Anti-CKAP4 antibody NBP1-85571 (A) shows similar protein distribution across tissues to independent antibody NBP1-85572 (B).
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FOXE1 Antibody
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Immunohistochemistry-Paraffin: FOXE1 Antibody [NB100-1271] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, AP-staining.
Immunohistochemistry-Paraffin: FOXE1 Antibody [NB100-1271] - Staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
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Immunohistochemistry: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP1-19788] - Analysis in mouse brain using DAB with hematoxylin counterstain.
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Immunohistochemistry-Paraffin: RPE65 Antibody (401.8B11.3D9) [NB100-355] - Analysis of FFPE human glioblastoma tissue section using 1:500 dilution of RPE65 [401.8B11.3D9] antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems).
Western Blot: RPE65 Antibody (401.8B11.3D9) [NB100-355] - Subcellular localization of BEST1 and surface Ca2+-dependent Cl- current in patient-derived iPSC-RPEs. Western blots show similar BEST1 expression levels in WT and patient-derived iPSC-RPEs. Each sample was from one cell lysis (BEST1 and beta-actin, RPE65 and CRALBP were on two gels, respectively). Image collected and cropped by CiteAb from the following publication (https://elifesciences.org/articles/29914), licensed under a CC-BY licence.
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Immunohistochemistry-Paraffin: UVRAG Antibody [NBP1-18885] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti- UVRAG used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: UVRAG Antibody [NBP1-18885] - Beclin 1 is acetylated at lysines 430 and 437. Mutations of K430 and K437 decrease the binding of Beclin 1 and Rubicon. Immunoprecipitation of indicated proteins with Beclin 1 in HEK293T cells transfected with indicated Beclin 1 constructs. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/ncomms8215), licensed under a CC-BY licence.
Western Blot: UVRAG Antibody [NBP1-18885] - Beclin 1 is acetylated at lysines 430 and 437. TSA and NAM increase the binding of Beclin 1 to Rubicon. Immunoprecipitation of indicated Beclin 1-binding partners with ectopically expressed Flag-Beclin 1 in HEK293T cells treated with TSA and NAM. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/ncomms8215), licensed under a CC-BY licence.
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TSHR Antibody
(484405) [Unconjugate
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Western blot shows lysates of human thyroid tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human TSH R Monoclonal Antibody (Catalog # MAB6534) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for TSH R at approximately 55 and 83 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.
Recombinant human TSH R protein was serially diluted 2-fold and captured by Mouse Anti-Human TSH R Monoclonal Antibody (Catalog # MAB65341) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human TSH R Monoclonal Antibody (Catalog # MAB6534) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Species: Hu
Applications: ELISA, WB
Synaptophysin
Antibody
NB300-653
Immunohistochemistry-Frozen: Synaptophysin Antibody [NB300-653] - Feline retina. Synaptophysin (green), DAPI (blue). The image was capture by epifluorescent microscopy. Image from verified customer review.
Western Blot: Synaptophysin Antibody [NB300-653] - Approximate MW of Synaptophysin is 39kDa.
Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - Rat brain, 1:100 dilution.
(2 Reviews)
(9 Publications)
Species: Ch, Fe, Hu, Pm, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
DDT Antibody
(OTI1H3)
NBP2-45677
Immunohistochemistry: DDT Antibody (1H3) [NBP2-45677] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: DDT Antibody (1H3) [NBP2-45677] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: DDT Antibody (1H3) [NBP2-45677] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Species: Hu, Pm
Applications: IHC, IHC-P, WB

Publications for TTF-1 / NKX2-1 Antibody (NBP2-44501) (0)

There are no publications for TTF-1 / NKX2-1 Antibody (NBP2-44501).
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Review for TTF-1 / NKX2-1 Antibody (NBP2-44501) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-44501:
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Immunocytochemistry TTF-1 / NKX2-1 NBP2-44501
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 ICC Mouse 05/30/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedThyroid tissue
SpeciesMouse
Lot7080-3p170314

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Immunocytochemistry TTF-1 / NKX2-1 NBP2-44501
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 Verified Customer
05/30/2017
Application: ICC
Species: Mouse
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Bioinformatics

Gene Symbol NKX2-1
Uniprot