TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690)

Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - Human Lung Carcinoma stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/690)

Immunohistochemistry: TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - TTF1 staining in thyroid tissue of wild-type C57 mouse (red). Dilution is 1:100. Image from a verified customer review.

Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - Human Thyroid stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/690)

• Reactivity: Hu, Mu, Rt
• Applications: Flow, IHC, IHC-P, ICC/IF
• Clone: 8G7G3/1 + NX2.1/690
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) Summary

• Immunogen: Rat full length TTF-1 recombinant protein (8G7G3/1); Recombinant TTF-1 protein (NX2.1/690)
• Localization: Nuclear
• Marker: Thyroid & Lung Epithelial Marker
• Specificity: Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates.Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: NKX2-1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
• Application Notes: Hu-chromosome location: 14q13.3
  Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. IHC reported in a verified customer review. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reported in a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690)

• BCH
• benign chorea
• BHC
• Homeobox protein NK-2 homolog A
• homeobox protein Nkx-2.1
• NK2 homeobox 1
• NK-2 homolog A
• NKX2.1
• NKX2-1
• NKX2A
• NKX2ANKX2.1
• T/EBP
• Thyroid nuclear factor 1
• Thyroid transcription factor 1BHC
• TITF1
• TITF1TEBP
• TTF1
• TTF-1
• TTF1BCH
• TTF-1NK-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for TTF-1 / NKX2-1 Antibody (NBP2-44501) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Feng Li ICC Mouse 05/30/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: Thyroid tissue
• Species: Mouse
• Lot: 7080-3p170314

Bioinformatics

• Gene Symbol: NKX2-1
• Uniprot:
  • P43699()