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TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690)

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Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - Human Lung Carcinoma stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/690)
Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - Human Lung Carcinoma stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/690)
Immunohistochemistry: TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - TTF1 staining in thyroid tissue of wild-type C57 mouse (red). Dilution is 1:100. Image from a verified customer review.
Immunohistochemistry-Paraffin: TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) [NBP2-44501] - Human Thyroid stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/690)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P, ICC/IF
Clone
8G7G3/1 + NX2.1/690
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-47773AF350
Alexa Fluor 405
NBP2-47773AF405
Alexa Fluor 488
NBP2-47773AF488
Alexa Fluor 532
NBP2-47773AF532
Alexa Fluor 594
NBP2-47773AF594
Alexa Fluor 647
NBP2-47773AF647
Alexa Fluor 700
NBP2-47773AF700
Alexa Fluor 750
NBP2-47773AF750
Allophycocyanin
NBP2-47773APC
Biotin
NBP2-47773B
DyLight 350
NBP2-47773UV
DyLight 405
NBP2-47773V
DyLight 488
NBP2-47773G
DyLight 550
NBP2-47773R
DyLight 594
NBP2-47773DL594
DyLight 650
NBP2-47773C
DyLight 680
NBP2-47773FR
DyLight 755
NBP2-47773IR
FITC
NBP2-47773F
HRP
NBP2-47773H
Janelia Fluor 549
NBP2-47773JF549
Janelia Fluor 646
NBP2-47773JF646
PE
NBP2-47773PE
PerCP
NBP2-47773PCP
View Available Formulations
Catalog# & Formulation Size Price
IHC-Prediluted
NBP2-48118
Azide and BSA Free
NBP2-47773-0.1mg
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TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690) Summary

Immunogen
Rat full length TTF-1 / NKX2-1 recombinant protein (8G7G3/1); Recombinant TTF-1 / NKX2-1 protein (NX2.1/690)
Localization
Nuclear
Marker
Thyroid & Lung Epithelial Marker
Specificity
Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates.Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.
Isotype
IgG1/IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NKX2-1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Immunofluorescence 0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
40 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-44501 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml (NBP2-47773).

Alternate Names for TTF-1 / NKX2-1 Antibody (8G7G3/1 + NX2.1/690)

  • BCH
  • benign chorea
  • BHC
  • Homeobox protein NK-2 homolog A
  • homeobox protein Nkx-2.1
  • NK2 homeobox 1
  • NK-2 homolog A
  • NKX2.1
  • NKX2-1
  • NKX2A
  • NKX2ANKX2.1
  • T/EBP
  • Thyroid nuclear factor 1
  • Thyroid transcription factor 1BHC
  • TITF1
  • TITF1TEBP
  • TTF1
  • TTF-1
  • TTF1BCH
  • TTF-1NK-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). Dilution is 1:100. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).
Immunohistochemistry: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Imaging of Mouse stomach tissue. Positive staining can only be observed inside the thyroid cells and the follicle in thyroid tissue. This image was submitted via customer Review.
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(1 Publication)
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NBP1-85599
Western Blot: Cytokeratin 20 Antibody [NBP1-85599] - Analysis in human small intestine tissue.
Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP1-85599] - Analysis in human small intestine and liver tissues. Corresponding KRT20 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP1-85599] - Staining of human liver shows no positivity in hepatocytes as expected
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Immunocytochemistry/Immunofluorescence: Substance P Antibody [NB300-187] - Staining of trigeminal ganglia from P3-P5 rat pups. BMC Neuroscience 2010, 11:32doi:10.1186/1471-2202-11-32
Immunohistochemistry: Substance P Antibody [NB300-187] - Spinal Cord Dorsal Horn (Human).
Immunocytochemistry/Immunofluorescence: Substance P Antibody [NB300-187] - Detection of Substance P in rat spinal cord dorsal horn (red fluorescence). DAPI (blue) was used as counter stain.
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Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).
Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Immunofluorescent staining of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (OV-TL12/30) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) [NBP2-44814] - Formalin-fixed, paraffin-embeddedhuman prsotatecarcinoma stained with Biotin-conjugated Cytokeratin 7 Mouse Monoclonal Antibody (OV-TL12/30).
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Human
SP-B/Surfactant Prote ...
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ELISA: Human SP-B/Surfactant Protein B ELISA Kit (Colorimetric) [NBP2-76690] - Standard Curve Reference
Species: Hu
Thyroid Peroxidase
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NBP1-80670
Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody [NBP1-80670] - Staining in human thyroid gland and cerebral cortex tissues using anti-TPO antibody. Corresponding TPO RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody [NBP1-80670] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody [NBP1-80670] - Staining of human thyroid gland shows high expression.
(2 Publications)
Species: Hu
Applications: WB, IHC, IHC-P
PAX8 Antibody
NBP1-32440
Western Blot: PAX8 Antibody [NBP1-32440] - 293T whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: PAX8 Antibody [NBP1-32440] - Stepwise differentiation of human iPSCs towards renal progenitor cells (RPCs). Schematic description of the two-stage protocol applied to iPSC-renal commitment. Immunofluorescence of iPSCs (derived from retroviral transfected dermal fibroblasts) exposed to differentiating media. Pictured are the IM and MM marker expressions as Wt1, Pax8, Pax2, Six2 and Sall1. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep08826), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: PAX8 Antibody [NBP1-32440] - Paraffin-embedded mouse liver. PAX8 antibody diluted at 1:500.
(9 Publications)
Species: Hu, Mu, Rt
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Prosurfactant
Protein C Antibody
NBP1-87201
Immunohistochemistry-Paraffin: Prosurfactant Protein C Antibody [NBP1-87201] - Analysis in human lung and kidney tissues using HPA010928 antibody. Corresponding SFTPC RNA-seq data are presented for the same tissues.
Western Blot: Prosurfactant Protein C Antibody [NBP1-87201] - Analysis in human lung tissue.
Immunohistochemistry-Paraffin: Prosurfactant Protein C Antibody [NBP1-87201] - Staining of human kidney shows no positivity in cells in glomeruli as expected.
Species: Hu
Applications: WB, IHC, IHC-P
CKAP4/p63 Antibody
NBP1-85572
Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85572] - Staining in human cervix, uterine and skeletal muscle tissues using anti-CKAP4 antibody. Corresponding CKAP4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85572] - Staining of human bone marrow, cerebral cortex, cervix, uterine and skeletal muscle using Anti-CKAP4 antibody NBP1-85572 (A) shows similar protein distribution across tissues to independent antibody NBP1-85571 (B).
Western Blot: CKAP4/p63 Antibody [NBP1-85572] - Analysis using Anti-CKAP4 antibody NBP1-85572 (A) shows similar pattern to independent antibody NBP1-85571 (B).
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(4 Reviews)
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EGFR Antibody
[Unconjugated]
AF231
EGFR was detected in immersion fixed frozen sections of human skin using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
EGFR was detected in immersion fixed A431 human epithelial carcinoma cell line using Goat Anti-Human EGFR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF231) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Immunohistochemistry-Paraffin: FOXE1 Antibody [NB100-1271] - Staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
Immunohistochemistry-Paraffin: FOXE1 Antibody [NB100-1271] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with Tris/EDTA buffer pH 9, AP-staining.
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beta-1,3-Glucuronyltra ...
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(1 Publication)
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(401.8B11.3D9)
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(7 Reviews)
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Western Blot: UVRAG Antibody [NBP1-18885] - Beclin 1 is acetylated at lysines 430 and 437. TSA and NAM increase the binding of Beclin 1 to Rubicon. Immunoprecipitation of indicated Beclin 1-binding partners with ectopically expressed Flag-Beclin 1 in HEK293T cells treated with TSA and NAM. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/ncomms8215), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: UVRAG Antibody [NBP1-18885] - Section of human colon carcinoma. Antibody: Affinity purified rabbit anti- UVRAG used at a dilution of 1:200 (1ug/ml). Detection: DAB
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TSHR Antibody
(484405) [Unconjugate
MAB6534
Western blot shows lysates of human thyroid tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human TSH R Monoclonal Antibody (Catalog # MAB6534) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for TSH R at approximately 55 and 83 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.
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Species: Hu
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Synaptophysin
Antibody
NB300-653
Western Blot: Synaptophysin Antibody [NB300-653] - Approximate MW of Synaptophysin is 39kDa.
Immunohistochemistry-Frozen: Synaptophysin Antibody [NB300-653] - Feline retina. Synaptophysin (green), DAPI (blue). The image was capture by epifluorescent microscopy. Image from verified customer review.
Immunohistochemistry-Paraffin: Synaptophysin Antibody [NB300-653] - Rat brain, 1:100 dilution.
(2 Reviews)
(6 Publications)
Species: Hu, Mu, Rt, Ch, Fe, Pm
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
DDT Antibody
(OTI1H3)
NBP2-45677
Western Blot: DDT Antibody (1H3) [NBP2-45677] - Analysis of extracts (35ug) from 9 different cell lines by using DDT monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: DDT Antibody (1H3) [NBP2-45677] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: DDT Antibody (1H3) [NBP2-45677] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DDT.
Species: Hu, Pm
Applications: WB, IHC

Publications for TTF-1 / NKX2-1 Antibody (NBP2-44501) (0)

There are no publications for TTF-1 / NKX2-1 Antibody (NBP2-44501).
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Review for TTF-1 / NKX2-1 Antibody (NBP2-44501) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP2-44501:
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Immunocytochemistry TTF-1 / NKX2-1 NBP2-44501
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reviewed by:
Feng Li
 ICC Mouse 05/30/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedThyroid tissue
SpeciesMouse
Lot7080-3p170314

Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for TTF-1 / NKX2-1 Antibody (NBP2-44501) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47773AF350
Alexa Fluor 405 NBP2-47773AF405
Alexa Fluor 488 NBP2-47773AF488
Alexa Fluor 532 NBP2-47773AF532
Alexa Fluor 594 NBP2-47773AF594
Alexa Fluor 647 NBP2-47773AF647
Alexa Fluor 700 NBP2-47773AF700
Alexa Fluor 750 NBP2-47773AF750
Allophycocyanin NBP2-47773APC
Biotin NBP2-47773B
DyLight 350 NBP2-47773UV
DyLight 405 NBP2-47773V
DyLight 488 NBP2-47773G
DyLight 550 NBP2-47773R
DyLight 594 NBP2-47773DL594
DyLight 650 NBP2-47773C
DyLight 680 NBP2-47773FR
DyLight 755 NBP2-47773IR
FITC NBP2-47773F
HRP NBP2-47773H
Janelia Fluor 549 NBP2-47773JF549
Janelia Fluor 646 NBP2-47773JF646
PE NBP2-47773PE
PerCP NBP2-47773PCP
Learn about Custom Antibody Labeling

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Bioinformatics Tool for TTF-1 / NKX2-1 Antibody (NBP2-44501)

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Diseases for TTF-1 / NKX2-1 Antibody (NBP2-44501)

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PTMs for TTF-1 / NKX2-1 Antibody (NBP2-44501)

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Immunocytochemistry TTF-1 / NKX2-1 NBP2-44501
Enlarge
 Feng Li
05/30/2017
Application: ICC
Species: Mouse
read this review

Bioinformatics

Gene Symbol NKX2-1
Uniprot