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SLC7A8 Antibody (OTI7D6)

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Western Blot: SLC7A8 Antibody (7D6) [NBP1-47989] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SLC7A8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates ...read more
Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Carcinoma of bladder tissue.
Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SLC7A8 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SLC7A8 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-SLC7A8 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-SLC7A8 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-SLC7A8 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded colon tissue using anti-SLC7A8 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded endometrium tissue using anti-SLC7A8 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded Ovary tissue using anti-SLC7A8mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] - Staining of paraffin-embedded pancreas tissue using anti-SLC7A8 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: SLC7A8 Antibody (7D6) [NBP1-47989] Staining of paraffin-embedded prostate tissue using anti-SLC7A8 mouse monoclonal antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI7D6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.8 mg/ml

SLC7A8 Antibody (OTI7D6) Summary

Immunogen
Full-length protein expressed in 293T cell transfected with human SLC7A8 expression vector
Specificity
This antibody is specific for Homo sapiens solute carrier family 7 (amino acid transporter, L-type), member 8 (SLC7A8), transcript variant 1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SLC7A8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:200-1:1000
Theoretical MW
58.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 2
using
NBP1-47989 in the following applications:

Publications
Read Publication using NBP1-47989.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.8 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SLC7A8 Antibody (OTI7D6)

  • hLAT2
  • integral membrane protein E16H
  • large neutral amino acids transporter small subunit 2
  • LAT2L-type amino acid transporter 2
  • LPI-PC1
  • solute carrier family 7 (amino acid transporter, L-type), member 8
  • Solute carrier family 7 member 8
  • y+ system), member 8

Background

Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine orD,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SLC7A8 Antibody (NBP1-47989)(1)

Review for SLC7A8 Antibody (NBP1-47989) (1) 21

Average Rating: 2
(Based on 1 review)
We have 1 review tested in 1 species: Bovine.

Reviews using NBP1-47989:
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(1)
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Images Ratings Applications Species Date Details
Western Blot SLC7A8 NBP1-47989
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2
reviewed by:
Alexis Hruby
WB Bovine 06/23/2021
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Summary

ApplicationWestern Blot
Sample TestedMammary Epithial Cell Line,whole cell lysate of mammary epithelial cell lines, Sample Amount: 30 ug
SpeciesBovine
LotA01

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.
Antibody was tested multiple times but two images are shown. I have attached two images, one where the antibody was run alone with a very high dilution 1:125. The other image is this antibody (LAT2) run with another antibody from another company that has a dark band (LAT1). The dilution for LAT2 in this case is 1:250. You can see the light bands located at the same locations as when LAT2 was run alone. This antibody was supposed to bind at an estimated 58 kDa. However, instead it binds under the LAT1 pictured (which is supposed to be 48 kDa) and above it (close to 70 kDa). It is unclear if these are reliable bands because they are so faint and in the wrong location; further, in order to see bands you need a very high volume of the antibody and so it becomes very expensive. In summary, I have rated the antibody as two stars.

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WB Video Protocol

FAQs for SLC7A8 Antibody (NBP1-47989). (Showing 1 - 1 of 1 FAQ).

  1. Our customer wants to know whether the item NBP1-47989 can apply to human follicular dendritic cell for IHC-paraffin.
    • We have tested this antibody in IHC-P in human tissue therefore I see no problem in using this in follicular dendritic cells. However there must be SLC7A8 Protein expressed in these cells.

Secondary Antibodies

 

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Recent Reviews

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Alexis Hruby
06/23/2021
Application: WB
Species: Bovine

Bioinformatics

Gene Symbol SLC7A8
Entrez