Recombinant Human sFRP-1 Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

Order Details

Recombinant Human sFRP-1 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. Ko, L. et al. (2002) Exp. Cell Res. 280:280. The ED50 for this effect is 0.15‑0.75 µg/mL.
Source
Mouse myeloma cell line, NS0-derived human sFRP-1 protein
Ser32-Lys314
Accession #
N-terminal Sequence
Ser32, Phe39 (minor) & Asp42 (minor)
Protein/Peptide Type
Recombinant Proteins
Gene
SFRP1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
32.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
33-40 kDa, reducing conditions
Publications
Read Publications using
5396-SF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Phosphate and NaCl.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 250 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human sFRP-1 Protein, CF

  • FRP1
  • FRP-1FrzA
  • FRPSARP-2
  • FrzA
  • SARP-2
  • SARP2sFRP-1
  • Secreted apoptosis-related protein 2
  • secreted frizzled-related protein 1
  • sFRP1
  • sFRP-1

Background

Secreted Frizzled Related Protein-1 (sFRP-1), also known as SARP-2, FRP, and FrzA, belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled transmembrane Wnt receptors. The sFRP proteins are approximately 30-35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-3). Mature human sFRP-1 shares 97% and 96% amino acid sequence identity with mouse and rat sFRP-1, respectively. It binds to Wnt-1, -2, -3a, and -4 and prevents their activation of canonical beta-catenin signaling (4-7). sFRP-1 is up-regulated in the ischemic heart where it limits inflammation and the severity of tissue damage following myocardial infarction (8-10). During angiogenesis it induces endothelial cell migration, tube formation, and vessel formation (11). sFRP-1 is expressed by bone marrow stromal cells and osteoclasts (12). It binds to TRANCE/RANK Ligand, inhibits osteoclast and osteoblast formation and survival, and inhibits the formation of trabecular bone (13, 14). sFRP-1 additionally restricts kidney tubule formation (7), the inflammatory activation of leukocytes (10), early B lymphoid progression (12), and vitreous fluid outflow in the eye (15).
  1. Cruciat, C.M. and C. Niehrs (2013) Cold Spring Harb. Perspect. Biol. 5:e015081.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Finch, P.W. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6770.
  4. Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.
  5. Dennis, S. et al. (1999) J. Cell Sci. 112:3815.
  6. Wawrzak, D. et al. (2007) Biochem. Biophys. Res. Commun. 357:1119.
  7. Yoshino, K. et al. (2001) Mech. Dev. 102:45.
  8. Barandon, L. et al. (2002) Circulation 108:2282.
  9. Sklepkiewicz, P. et al. (2015) Circ. Heart Fail. 8:362.
  10. Barandon, L. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:e80.
  11. Dufourcq, P. et al. (2002) Circ. Res. 106:3097.
  12. Yokota, T. et al. (2008) J. Immunol. 181:6061.
  13. Hausler, K.D. et al. (2004) J. Bone Miner. Res. 19:1873.
  14. Bodine, P.V.N. et al. (2004) Mol. Endocrinol. 18:1222.
  15. Wang, W.-H. et al. (2008) J. Clin. Invest. 118:1056.

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Publications for sFRP-1 (5396-SF)(11)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 2 applications: Bioassay, In Vivo.


Filter By Application
Bioassay
(10)
In Vivo
(1)
All Applications
Filter By Species
Human
(4)
Mouse
(3)
Rat
(3)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 11. Show All 11 Publications.
Publications using 5396-SF Applications Species
F McLeod, A Bossio, A Marzo, L Ciani, S Sibilla, S Hannan, GA Wilson, E Palomer, TG Smart, A Gibb, PC Salinas Wnt Signaling Mediates LTP-Dependent Spine Plasticity and AMPAR Localization through Frizzled-7 Receptors Cell Rep, 2018;23(4):1060-1071. 2018 [PMID: 29694885] (Bioassay, Rat) Bioassay Rat
J Steinfeld, I Steinfeld, A Bausch, N Coronato, ML Hampel, H Depner, PG Layer, A Vogel-Höpk BMP-induced reprograming of the retina into RPE requires WNT signalling in the developing chick optic cup Biol Open, 2017;0(0):. 2017 [PMID: 28546339] (In Vivo, Chicken) In Vivo Chicken
T Nakamura, T Hosoyama, J Murakami, M Samura, K Ueno, H Kurazumi, R Suzuki, A Mikamo, K Hamano Age-related increase in Wnt inhibitor causes a senescence-like phenotype in human cardiac stem cells Biochem. Biophys. Res. Commun., 2017;0(0):. 2017 [PMID: 28435069] (Bioassay, Human) Bioassay Human
K Minegishi, M Hashimoto, R Ajima, K Takaoka, K Shinohara, Y Ikawa, H Nishimura, AP McMahon, K Willert, Y Okada, H Sasaki, D Shi, T Fujimori, T Ohtsuka, Y Igarashi, TP Yamaguchi, A Shimono, H Shiratori, H Hamada A Wnt5 Activity Asymmetry and Intercellular Signaling via PCP Proteins Polarize Node Cells for Left-Right Symmetry Breaking Dev. Cell, 2017;40(5):439-452.e4. 2017 [PMID: 28292423] (Bioassay, Mouse) Bioassay Mouse
Holger S Willenberg Endothelial cells regulate ?-catenin activity in adrenocortical cells via secretion of basic fibroblast growth factor Mol. Cell. Endocrinol., 2016;0(0):. 2016 [PMID: 27889473] (Bioassay, Human) Bioassay Human
Adipose- and muscle-derived Wnts trigger pancreatic ?-cell adaptation to systemic insulin resistance Sci Rep, 2016;6(0):31553. 2016 [PMID: 27527335] (Bioassay, Rat) Bioassay Rat
Arensman M, Kovochich A, Kulikauskas R, Lay A, Yang P, Li X, Donahue T, Major M, Moon R, Chien A, Dawson D WNT7B mediates autocrine Wnt/beta-catenin signaling and anchorage-independent growth in pancreatic adenocarcinoma. Oncogene, 2014;33(7):899-908. 2014 [PMID: 23416978] (Bioassay, Human) Bioassay Human
Dawes L, Sugiyama Y, Tanedo A, Lovicu F, McAvoy J Wnt-frizzled signaling is part of an FGF-induced cascade that promotes lens fiber differentiation. Invest Ophthalmol Vis Sci, 2013;54(3):1582-90. 2013 [PMID: 23385791] (Bioassay, Rat) Bioassay Rat
Patel N, Sharpe PT, Miletich I Coordination of epithelial branching and salivary gland lumen formation by Wnt and FGF signals. Dev. Biol., 2011;358(1):156-67. 2011 [PMID: 21806977] (Bioassay, Mouse) Bioassay Mouse
Scheel C, Eaton E, Li S, Chaffer C, Reinhardt F, Kah K, Bell G, Guo W, Rubin J, Richardson A, Weinberg R Paracrine and autocrine signals induce and maintain mesenchymal and stem cell states in the breast. Cell, 2011;145(6):926-40. 2011 [PMID: 21663795] (Bioassay, Human) Bioassay Human
Show All 11 Publications.

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Bioinformatics

Gene Symbol SFRP1
Entrez
Uniprot