Recombinant Human sFRP-1 Protein, CF


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Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human sFRP-1 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. Ko, L. et al. (2002) Exp. Cell Res. 280:280. The ED50 for this effect is 0.15‑0.75 µg/mL.
Mouse myeloma cell line, NS0-derived human sFRP-1 protein
Accession #
N-terminal Sequence
Ser32, Phe39 (minor) & Asp42 (minor)
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
32.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
33-40 kDa, reducing conditions
Read Publications using
5396-SF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Phosphate and NaCl.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 250 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human sFRP-1 Protein, CF

  • FRP1
  • FRP-1FrzA
  • FrzA
  • SARP-2
  • SARP2sFRP-1
  • Secreted apoptosis-related protein 2
  • secreted frizzled-related protein 1
  • sFRP1
  • sFRP-1


Secreted Frizzled Related Protein-1 (sFRP-1), also known as SARP-2, FRP, and FrzA, belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled transmembrane Wnt receptors. The sFRP proteins are approximately 30-35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-3). Mature human sFRP-1 shares 97% and 96% amino acid sequence identity with mouse and rat sFRP-1, respectively. It binds to Wnt-1, -2, -3a, and -4 and prevents their activation of canonical beta-catenin signaling (4-7). sFRP-1 is up-regulated in the ischemic heart where it limits inflammation and the severity of tissue damage following myocardial infarction (8-10). During angiogenesis it induces endothelial cell migration, tube formation, and vessel formation (11). sFRP-1 is expressed by bone marrow stromal cells and osteoclasts (12). It binds to TRANCE/RANK Ligand, inhibits osteoclast and osteoblast formation and survival, and inhibits the formation of trabecular bone (13, 14). sFRP-1 additionally restricts kidney tubule formation (7), the inflammatory activation of leukocytes (10), early B lymphoid progression (12), and vitreous fluid outflow in the eye (15).
  1. Cruciat, C.M. and C. Niehrs (2013) Cold Spring Harb. Perspect. Biol. 5:e015081.
  2. van Amerongen, R. and R. Nusse (2009) Development 136:3205.
  3. Finch, P.W. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6770.
  4. Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.
  5. Dennis, S. et al. (1999) J. Cell Sci. 112:3815.
  6. Wawrzak, D. et al. (2007) Biochem. Biophys. Res. Commun. 357:1119.
  7. Yoshino, K. et al. (2001) Mech. Dev. 102:45.
  8. Barandon, L. et al. (2002) Circulation 108:2282.
  9. Sklepkiewicz, P. et al. (2015) Circ. Heart Fail. 8:362.
  10. Barandon, L. et al. (2011) Arterioscler. Thromb. Vasc. Biol. 31:e80.
  11. Dufourcq, P. et al. (2002) Circ. Res. 106:3097.
  12. Yokota, T. et al. (2008) J. Immunol. 181:6061.
  13. Hausler, K.D. et al. (2004) J. Bone Miner. Res. 19:1873.
  14. Bodine, P.V.N. et al. (2004) Mol. Endocrinol. 18:1222.
  15. Wang, W.-H. et al. (2008) J. Clin. Invest. 118:1056.

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Publications for sFRP-1 (5396-SF)(15)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 2 applications: Bioassay, In Vivo.

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In Vivo
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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using 5396-SF Applications Species
DA Jeffrey, J Pires Da S, AM Garcia, X Jiang, A Karimpour-, LS Toni, T Lanzicher, B Peña, CA Miyano, K Nunley, A Korst, O Sbaizero, MR Taylor, SD Miyamoto, BL Stauffer, CC Sucharov Serum circulating proteins from pediatric dilated cardiomyopathy patients cause pathologic remodeling and cardiomyocyte stiffness JCI Insight, 2021-10-08;0(0):. 2021-10-08 [PMID: 34383712] (Bioassay, Rat) Bioassay Rat
Y Park, M Lofton, D Li, MR Rasin Extrinsic Regulators of mRNA Translation in Developing Brain: Story of WNTs Cells, 2021-01-28;10(2):. 2021-01-28 [PMID: 33525513] (Bioassay, Mouse) Bioassay Mouse
SH Cho, IY Kuo, PF Lu, HT Tzeng, WW Lai, WC Su, YC Wang Rab37 mediates exocytosis of secreted frizzled-related protein 1 to inhibit Wnt signaling and thus suppress lung cancer stemness Cell Death Dis, 2018-08-29;9(9):868. 2018-08-29 [PMID: 30158579] (Bioassay, Human) Bioassay Human
NJ Hawkshaw, JA Hardman, IS Haslam, A Shahmalak, A Gilhar, X Lim, R Paus Identifying novel strategies for treating human hair loss disorders: Cyclosporine A suppresses the Wnt inhibitor, SFRP1, in the dermal papilla of human scalp hair follicles PLoS Biol., 2018-05-08;16(5):e2003705. 2018-05-08 [PMID: 29738529] (Bioassay, Human) Bioassay Human
F McLeod, A Bossio, A Marzo, L Ciani, S Sibilla, S Hannan, GA Wilson, E Palomer, TG Smart, A Gibb, PC Salinas Wnt Signaling Mediates LTP-Dependent Spine Plasticity and AMPAR Localization through Frizzled-7 Receptors Cell Rep, 2018-04-24;23(4):1060-1071. 2018-04-24 [PMID: 29694885] (Bioassay, Rat) Bioassay Rat
J Steinfeld, I Steinfeld, A Bausch, N Coronato, ML Hampel, H Depner, PG Layer, A Vogel-Höpk BMP-induced reprograming of the retina into RPE requires WNT signalling in the developing chick optic cup Biol Open, 2017-07-15;0(0):. 2017-07-15 [PMID: 28546339] (In Vivo, Chicken) In Vivo Chicken
T Nakamura, T Hosoyama, J Murakami, M Samura, K Ueno, H Kurazumi, R Suzuki, A Mikamo, K Hamano Age-related increase in Wnt inhibitor causes a senescence-like phenotype in human cardiac stem cells Biochem. Biophys. Res. Commun., 2017-04-21;0(0):. 2017-04-21 [PMID: 28435069] (Bioassay, Human) Bioassay Human
K Minegishi, M Hashimoto, R Ajima, K Takaoka, K Shinohara, Y Ikawa, H Nishimura, AP McMahon, K Willert, Y Okada, H Sasaki, D Shi, T Fujimori, T Ohtsuka, Y Igarashi, TP Yamaguchi, A Shimono, H Shiratori, H Hamada A Wnt5 Activity Asymmetry and Intercellular Signaling via PCP Proteins Polarize Node Cells for Left-Right Symmetry Breaking Dev. Cell, 2017-03-13;40(5):439-452.e4. 2017-03-13 [PMID: 28292423] (Bioassay, Mouse) Bioassay Mouse
Holger S Willenberg Endothelial cells regulate ?-catenin activity in adrenocortical cells via secretion of basic fibroblast growth factor Mol. Cell. Endocrinol., 2016-11-23;0(0):. 2016-11-23 [PMID: 27889473] (Bioassay, Human) Bioassay Human
Adipose- and muscle-derived Wnts trigger pancreatic ?-cell adaptation to systemic insulin resistance Sci Rep, 2016-08-16;6(0):31553. 2016-08-16 [PMID: 27527335] (Bioassay, Rat) Bioassay Rat
Show All 15 Publications.

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Gene Symbol SFRP1