CD9 Antibody (SA35-08)

Western Blot: CD9 Antibody (SA35-08) [NBP2-67310] - Exosome or whole cell lysates (WCL) sample from MDA-MB-231 cells was loaded with 10 ug/lane. 10% SDS-PAGE. CD9 Antibody (NBP2-67310) was used for primary antibody: 1:1000, 4C, overnight. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: CD9 Antibody (SA35-08) [NBP2-67310] - Staining CD9 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310] - Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m

Immunocytochemistry/Immunofluorescence: CD9 Antibody (SA35-08) [NBP2-67310] - Staining CD9 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310] - Analysis of paraffin-embedded human kidney tissue using anti-CD9 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310] - Analysis of paraffin-embedded human spleen tissue using anti-CD9 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310] - Analysis of paraffin-embedded human tonsil tissue using anti-CD9 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310] - Analysis of paraffin-embedded mouse brain tissue using anti-CD9 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310] - Analysis of paraffin-embedded mouse spleen tissue using anti-CD9 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m

Immunohistochemistry-Paraffin: CD9 Antibody (SA35-08) [NBP2-67310] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD9 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: SA35-08
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

CD9 Antibody (SA35-08) Summary

• Description: Novus Biologicals Rabbit CD9 Antibody (SA35-08) (NBP2-67310) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. Anti-CD9 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human CD9 aa 179-228 / 228. (SwissProt: P21926 Human; SwissProt: P40240 Mouse; SwissProt: P40241 Rat)
• Epitope: cytoplasmic
• Localization: Cell membrane, Membrane
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD9
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD9 Antibody (SA35-08)

• BA2
• BA-2/p24 antigen
• BTCC-1
• CD9 antigen
• CD9 molecule
• CD9
• Cell growth-inhibiting gene 2 protein
• DRAP-27
• Leukocyte antigen MIC3
• MIC3
• motility related protein-1,5H9 antigen
• Motility-related protein
• MRP-1FLJ99568
• p24
• tetraspanin-29
• TSPAN29
• TSPAN-29
• TSPAN29MIC3CD9 antigen (p24)

Background

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behavior in peripheral nerve.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD9 Antibody (NBP2-67310)(2)

Publications using NBP2-67310

• Cabiati M, Randazzo E, Guiducci L et al. Evaluation of Exosomal Coding and Non-Coding RNA Signature in Obese Adolescents International Journal of Molecular Sciences 2022-12-21 [PMID: 36613584]
• Sadri M, Hirosawa N, Le J et al. Tumor necrosis factor receptor-1 is selectively sequestered into Schwann cell extracellular vesicles where it functions as a TNF alpha decoy Glia 2021-09-24 [PMID: 34559433]

Review for CD9 Antibody (NBP2-67310) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Yongkang Yang WB Human 10/23/2022

Summary

• Application: Western Blot
• Sample Tested: MDA MB 231 whole cell lysates and conditioned medium
• Species: Human
• Lot: H01224

Bioinformatics

• Gene Symbol: CD9