CD151 Antibody (11G5a)

Images

 
Flow Cytometry: CD151 Antibody (11G5a) [NB100-64810] - Staining of human peripheral blood platelets with MOUSE ANTI HUMAN CD151.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-Fr, IHC-P, IP
Clone
11G5a
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

CD151 Antibody (11G5a) Summary

Immunogen
Made to Human CD151
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD151
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
For IHC: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. For Flow Cytometry: Use 10ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood.
Control
CD151 Overexpression Lysate
CD151 Knockout 293T Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD151 Antibody (11G5a)

  • CD151 antigen (Raph blood group)
  • CD151 antigenplatelet surface glycoprotein gp27
  • CD151 molecule (Raph blood group)
  • CD151
  • GP27
  • Membrane glycoprotein SFA-1
  • MER2
  • PETA3
  • PETA-3
  • PETA-3SFA-1
  • platelet-endothelial cell tetraspan antigen 3
  • Platelet-endothelial tetraspan antigen 3
  • RAPH
  • SFA1
  • SFA-1
  • tetraspanin-24
  • TSPAN24
  • tspan-24
  • TSPAN24hemidesmosomal tetraspanin CD151

Background

CD151 is essential for the proper assembly of the glomerular and tubular basement membranes in kidney. It interacts with integrins alpha3beta1, alpha5beta1, alpha3beta1 and alpha6beta4, and with CD9 and CD181. It is expressed in a variety of tissues including vascular endothelium and epidermis, platelets, monocytes and also erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes. CD151 is however not expressed by lymphocytes or granulocytes. Defects in CD151 are the cause of nephropathy with pretibial epidermolysis bullosa and deafness.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD151 Antibody (NB100-64810) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-64810AF350
Alexa Fluor 405 NB100-64810AF405
Alexa Fluor 488 NB100-64810AF488
Alexa Fluor 532 NB100-64810AF532
Alexa Fluor 594 NB100-64810AF594
Alexa Fluor 647 NB100-64810AF647
Alexa Fluor 700 NB100-64810AF700
Alexa Fluor 750 NB100-64810AF750
Allophycocyanin NB100-64810APC
Allophycocyanin/Cy7 NB100-64810APCCY7
Biotin NB100-64810B
DyLight 350 NB100-64810UV
DyLight 405 NB100-64810V
DyLight 488 NB100-64810G
DyLight 550 NB100-64810R
DyLight 594 NB100-64810DL594
DyLight 650 NB100-64810C
DyLight 680 NB100-64810FR
DyLight 755 NB100-64810IR
FITC NB100-64810F
HRP NB100-64810H
Janelia Fluor 549 NB100-64810JF549
Janelia Fluor 646 NB100-64810JF646
PE NB100-64810PE
PE/Atto594 NB100-64810PEATT594
PE/Cy5.5 NB100-64810PECY55
PE/Cy7 NB100-64810PECY7
PerCP NB100-64810PCP

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Bioinformatics

Gene Symbol CD151
Entrez
Uniprot
COVID-19 update