CD151 Antibody (11G5a) - BSA Free

Flow Cytometry: CD151 Antibody (11G5a) [NB100-64810] - Staining of human peripheral blood platelets with MOUSE ANTI HUMAN CD151.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, IHC-Fr, IHC-P, IP
• Clone: 11G5a
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CD151 Antibody (11G5a) - BSA Free Summary

• Additional Information: Clone 11G5a was used by HLDA to establish CD designation.
• Immunogen: Made to Human CD151
• Kit Type: Activity Assay Kit
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD151
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000
• Application Notes: For IHC: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. For Flow Cytometry: Use 10ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood. Western Blot: Clone 11G5a recognises human CD151 under non-reducing conditions.
• Control:

  CD151 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CD151 Antibody (11G5a) - BSA Free

• CD151 antigen (Raph blood group)
• CD151 antigenplatelet surface glycoprotein gp27
• CD151 molecule (Raph blood group)
• CD151
• GP27
• Membrane glycoprotein SFA-1
• MER2
• PETA3
• PETA-3
• PETA-3SFA-1
• platelet-endothelial cell tetraspan antigen 3
• Platelet-endothelial tetraspan antigen 3
• RAPH
• SFA1
• SFA-1
• tetraspanin-24
• TSPAN24
• tspan-24
• TSPAN24hemidesmosomal tetraspanin CD151

Background

CD151 is essential for the proper assembly of the glomerular and tubular basement membranes in kidney. It interacts with integrins alpha3beta1, alpha5beta1, alpha3beta1 and alpha6beta4, and with CD9 and CD181. It is expressed in a variety of tissues including vascular endothelium and epidermis, platelets, monocytes and also erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes. CD151 is however not expressed by lymphocytes or granulocytes. Defects in CD151 are the cause of nephropathy with pretibial epidermolysis bullosa and deafness.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD151
• Entrez:
  • 977(Human)
• Uniprot:
  • P48509()