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CD151 Antibody (11G5a)

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Flow Cytometry: CD151 Antibody (11G5a) [NB100-64810] - Staining of human peripheral blood platelets with MOUSE ANTI HUMAN CD151.
Flow Cytometry: CD151 Antibody (11G5a) [NB100-64810] - Staining of human peripheral blood platelets with MOUSE ANTI HUMAN CD151.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-Fr, IHC-P, IP
Clone
11G5a
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NB100-64810AF350
Alexa Fluor 405
NB100-64810AF405
Alexa Fluor 488
NB100-64810AF488
Alexa Fluor 532
NB100-64810AF532
Alexa Fluor 594
NB100-64810AF594
Alexa Fluor 647
NB100-64810AF647
Alexa Fluor 700
NB100-64810AF700
Alexa Fluor 750
NB100-64810AF750
Allophycocyanin
NB100-64810APC
Allophycocyanin/Cy7
NB100-64810APCCY7
Biotin
NB100-64810B
DyLight 350
NB100-64810UV
DyLight 405
NB100-64810V
DyLight 488
NB100-64810G
DyLight 550
NB100-64810R
DyLight 594
NB100-64810DL594
DyLight 650
NB100-64810C
DyLight 680
NB100-64810FR
DyLight 755
NB100-64810IR
FITC
NB100-64810F
HRP
NB100-64810H
Janelia Fluor 549
NB100-64810JF549
Janelia Fluor 646
NB100-64810JF646
PE
NB100-64810PE
PE/Atto594
NB100-64810PEATT594
PE/Cy5.5
NB100-64810PECY55
PE/Cy7
NB100-64810PECY7
PerCP
NB100-64810PCP
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CD151 Antibody (11G5a) Summary

Immunogen
Made to Human CD151
Kit Type
Activity Assay Kit
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CD151
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
For IHC: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. For Flow Cytometry: Use 10ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood. Western Blot: Clone 11G5a recognises human CD151 under non-reducing conditions.
Control
CD151 Overexpression Lysate
CD151 Knockout 293T Cell Lysate

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for CD151 Antibody (11G5a)

  • CD151 antigen (Raph blood group)
  • CD151 antigenplatelet surface glycoprotein gp27
  • CD151 molecule (Raph blood group)
  • CD151
  • GP27
  • Membrane glycoprotein SFA-1
  • MER2
  • PETA3
  • PETA-3
  • PETA-3SFA-1
  • platelet-endothelial cell tetraspan antigen 3
  • Platelet-endothelial tetraspan antigen 3
  • RAPH
  • SFA1
  • SFA-1
  • tetraspanin-24
  • TSPAN24
  • tspan-24
  • TSPAN24hemidesmosomal tetraspanin CD151

Background

CD151 is essential for the proper assembly of the glomerular and tubular basement membranes in kidney. It interacts with integrins alpha3beta1, alpha5beta1, alpha3beta1 and alpha6beta4, and with CD9 and CD181. It is expressed in a variety of tissues including vascular endothelium and epidermis, platelets, monocytes and also erythroid cells, with a higher level of expression in erythroid precursors than on mature erythrocytes. CD151 is however not expressed by lymphocytes or granulocytes. Defects in CD151 are the cause of nephropathy with pretibial epidermolysis bullosa and deafness.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.
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Western Blot: CD63 Antibody (H5C6) [NBP2-42225] - THP1 whole cell protein was separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was probed with anti-CD63 antibody at 2 ug/mL and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Immunocytochemistry/Immunofluorescence: CD63 Antibody (H5C6) [NBP2-42225] - The CD63 (H5C6) antibody was tested in HeLa cells at a 1:50 dilution against DyLight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.
Flow Cytometry: CD63 Antibody (H5C6) [NBP2-42225] - An intracellular stain was performed on SK-MEL-28 cells with CD63 (H5C6) Antibody NBP2-42225R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
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CD81 Antibody (1D6)
NB100-65805
Western Blot: CD81 Antibody (1D6) [NB100-65805] - Isolation and characterization of exosomes from cell culture supernatant and human blood. Immunoblot of exosomal markers CD81 (~30 kDa), CD63 (~55 kDa), and LAMP2B (~50 kDa) in exosomes (5 ug lysates) isolated from the serum of breast cancer patients and healthy control. Grp94 (~100 kDa) and beta-actin (~42 kDa) serve as a negative control and a loading control, respectively. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep36502), licensed under a CC-BY licence.
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CD82/Kai-1
Antibody (C33)
NBP2-21792
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NBP2-67327
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Antibody - Pan Specif
AF887
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NBP2-36561
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Immunohistochemistry-Frozen: Fibronectin Antibody [NBP1-91258] - Analysis in a 6 month old Alport mouse kidney. Image from a verified customer review.
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(191801) [Unconjugate
MAB1230
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Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and MCF‑7 human breast cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for ERK2 at approximately 43 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human/Mouse/Rat ERK2 Monoclonal Antibody (Catalog # MAB1230). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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HGF [Unconjugated]
294-HG
Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. The ED50 for this effect is 0.1‑0.5 ng/mL.
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Tetraspanin-4
Antibody
NBP1-59438
Western Blot: Tetraspanin-4 Antibody [NBP1-59438] - Jurkat cell lysate, concentration 0.2-1 ug/ml.
Immunohistochemistry: Tetraspanin-4 Antibody [NBP1-59438] - Human Adult Liver Observed Staining: Cytoplasm in hepatocytes Primary Antibody Concentration: 1 : 600 Secondary Antibody: Donkey anti-Rabbit-Cy3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Exposure Time: 0.5 2.0 sec.
Western Blot: Tetraspanin-4 Antibody [NBP1-59438] - GST-Teraspanin EC2 Expressed in E. coli.
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EMMPRIN/CD147
Antibody [Unconjugate
AF972
Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for EMMPRIN/CD147 at approximately38-50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
EMMPRIN/CD147 was detected in immersion fixed paraffin-embedded sections of human cervix using Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Western blot shows lysates of HEK293T human embryonic kidney parental cell line and EMMPRIN/CD147 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EMMPRIN/CD147 at approximately 51 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
(1 Review)
(8 Publications)
Species: Hu
Applications: WB, Simple Western, ELISA, IHC, KO

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Product General Protocols

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FAQs for CD151 Antibody (NB100-64810) (0)

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Control Lysate(s)

CD151 Overexpression Lysate
CD151 Knockout 293T Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-64810AF350
Alexa Fluor 405 NB100-64810AF405
Alexa Fluor 488 NB100-64810AF488
Alexa Fluor 532 NB100-64810AF532
Alexa Fluor 594 NB100-64810AF594
Alexa Fluor 647 NB100-64810AF647
Alexa Fluor 700 NB100-64810AF700
Alexa Fluor 750 NB100-64810AF750
Allophycocyanin NB100-64810APC
Allophycocyanin/Cy7 NB100-64810APCCY7
Biotin NB100-64810B
DyLight 350 NB100-64810UV
DyLight 405 NB100-64810V
DyLight 488 NB100-64810G
DyLight 550 NB100-64810R
DyLight 594 NB100-64810DL594
DyLight 650 NB100-64810C
DyLight 680 NB100-64810FR
DyLight 755 NB100-64810IR
FITC NB100-64810F
HRP NB100-64810H
Janelia Fluor 549 NB100-64810JF549
Janelia Fluor 646 NB100-64810JF646
PE NB100-64810PE
PE/Atto594 NB100-64810PEATT594
PE/Cy5.5 NB100-64810PECY55
PE/Cy7 NB100-64810PECY7
PerCP NB100-64810PCP
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol CD151
Entrez
Uniprot