CD9 Antibody (PSH0-95)

Immunohistochemistry: CD9 Antibody (PSH0-95) [NBP3-32171] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-CD9 antibody (NBP3-32171) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32171) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: CD9 Antibody (PSH0-95) [NBP3-32171] - Western blot analysis of CD9 on different lysates with Rabbit anti-CD9 antibody (NBP3-32171) at 1/5,000 dilution.Lane 1: HeLa cell lysateLane 2: K-562 cell lysate (negative)Lane 3: MCF7 cell lysateLane 4: HepG2 cell lysateLysates/proteins at 20 µg/Lane.Predicted band size: 25 kDaObserved band size: 20 kDaExposure time: Lane 1-4 (left): 53 seconds; Lane 1-4 (right): 3 minutes;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: PSH0-95
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

CD9 Antibody (PSH0-95) Summary

• Description: Novus Biologicals Rabbit CD9 Antibody (PSH0-95) (NBP3-32171) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within human CD9 aa 196-228 / 228. (Uniprot: P21926)
• Localization: Cell membrane, Membrane, Secreted, extracellular exosome.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CD9
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:2000-1:5000
• Theoretical MW: 25 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for CD9 Antibody (PSH0-95)

• BA2
• BA-2/p24 antigen
• BTCC-1
• CD9 antigen
• CD9 molecule
• CD9
• Cell growth-inhibiting gene 2 protein
• DRAP-27
• Leukocyte antigen MIC3
• MIC3
• motility related protein-1,5H9 antigen
• Motility-related protein
• MRP-1FLJ99568
• p24
• tetraspanin-29
• TSPAN29
• TSPAN-29
• TSPAN29MIC3CD9 antigen (p24)

Background

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behavior in peripheral nerve.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CD9