CD9 Antibody (MEM-61) [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB, ELISA, Flow, Func, IHC, CyTOF-ready
• Clone: MEM-61
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

CD9 Antibody (MEM-61) [Alexa Fluor® 488] Summary

• Additional Information: Clone MEM-61 was used by HLDA to establish CD designation.
• Immunogen: Pre-B cell line NALM-6
• Specificity: The antibody MEM-61 recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes. HLDA VI; WS Code P P-15
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD9
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • In vitro assay
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for CD9 Antibody (MEM-61) [Alexa Fluor® 488]

• BA2
• BA-2/p24 antigen
• BTCC-1
• CD9 antigen
• CD9 molecule
• CD9
• Cell growth-inhibiting gene 2 protein
• DRAP-27
• Leukocyte antigen MIC3
• MIC3
• motility related protein-1,5H9 antigen
• Motility-related protein
• MRP-1FLJ99568
• p24
• tetraspanin-29
• TSPAN29
• TSPAN-29
• TSPAN29MIC3CD9 antigen (p24)

Background

CD9 antigen is a glycoprotein expressed on the surface of developing B lymphocytes, platelets, monocytes, eosinophils, basophil, stimulated T lymphocytes and by neurons and glial cells in the peripheral nervous system. It belongs to a family of membrane proteins termed tetraspanins which transverse the membrane four times. In pre B cells and platelets, CD9 antigen regulates cell activation and aggregation possibly through an association with the integrin CD41 / CD61 (GPIIb / GPIIIa). It also regulates cell motility in a variety of cell lines, and appears to be an important regulator of Schwann cell behavior in peripheral nerve.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD9 Antibody (NB500-327AF488)(2)

Publications using NB500-327AF488

• Zhou J, Wu Z, Hu J et al High-throughput single-EV liquid biopsy: Rapid, simultaneous, and multiplexed detection of nucleic acids, proteins, and their combinations Sci Adv 2020-11-21 [PMID: 33219024]
  
  Details:
  Citation using the Alexa Fluor 488 version of this antibody.
• Han C, Kang M, Kang H et al. Characterization of extracellular vesicle and virus-like particles by single vesicle tetraspanin analysis Sensors and Actuators B: Chemical 2023-05-01 [PMID: 30236130] (ICC/IF, Human)

Bioinformatics

• Gene Symbol: CD9