CD45RC Antibody (OX22) - BSA Free

Flow Cytometry: CD45RC Antibody (OX22) [NB120-6406] - Analysis using the FITC conjugate of NB120-6406. Staining of rat spleen cells.

Flow Cytometry: CD45RC Antibody (OX22) [NB120-6406] - Analysis using the Biotin conjugate of NB120-6406. Staining of rat splenocytes.

• Reactivity: Rt
• Applications: Flow, IHC
• Clone: OX22
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CD45RC Antibody (OX22) - BSA Free Summary

• Immunogen: Rat lymphocytes stimulated in culture with phytohaemagglutinin.
• Localization: Cell Membrane
• Specificity: NB120-6406 recognizes rat CD45RC, the high molecular weight form of the leucocyte common antigen. This antigen is found on B cells, approximately 50% of bone marrow cells, all CD8+ve T cells, but splits CD4+ve T cells into two populations,CD4+ CD45RC (Th 1-like) and CD4+ CD45RC low (Th-2 like). This product is routinely tested in flow cytometry on rat splenocytes.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRC
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CD45RC Antibody (OX22) - BSA Free

• B220
• CD45 antigen
• CD45R
• EC 3.1.3.48
• L-CA
• LY5
• protein tyrosine phosphatase, receptor type, C
• receptor-type tyrosine-protein phosphatase C
• T200 glycoprotein
• T200 leukocyte common antigen
• T200receptor type, c polypeptide

Background

The CD45 family of cell surface glycoprotein antigens exist as a set of isoforms. The patterns of monoclonal antibody recognition of the CD45 phenotypes can be restricted (CD45RA, RB and RC), or broader, such as the CD45 T 200 species. The "restricted" subsets of this group arise as a result of translation of alternatively spliced mRNAs from exons A, B and C (exons 4, 5 and 6), where exons 3-15 encode the extracellular domain of the CD45 molecule. The restricted epitopes are differentially expressed on T and B lymphocytes, including functionally distinct T cells. CD45 (LCA) is a transmembrane phosphotyrosine phosphatase expressed on leucocytes. Myeloid cells do not express the RC isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD45RC Antibody (NB120-6406)(2)

Publications using NB120-6406

• Hancock WW, Khoury SJ, Carpenter CB, Sayegh MH. Differential effects of oral versus intrathymic administration of polymorphic major histocompatibility complex class II peptides on mononuclear and endothelial cell activation and cytokine expression during a delayed-type hypersensitivity response. Am J Pathol. [PMID: 8203456] (IHC-Fr, Rat)
• Kampinga J, Schuurman HJ, Pol GH et al. Vascular thymus transplantation in rats. Technique, morphology, and function. Transplantation. 1990-10-01 [PMID: 2219290]

Bioinformatics

• Gene Symbol: PTPRC