CD45RC Antibody (OX-22) [FITC]

Flow Cytometry: CD45RC Antibody (OX22) [FITC] [NB100-63850] - Staining of rat spleen cells with MOUSE.ANTI RAT CD45RC:FITC

• Reactivity: Rt
• Applications: Flow
• Clone: OX-22
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: FITC
• Concentration: 0.1 mg/ml

CD45RC Antibody (OX-22) [FITC] Summary

• Immunogen: Phytohaemagglutinin (PHA) -activated rat lymphocytes
• Localization: Cell Membrane
• Specificity: NB120-6406 recognizes rat CD45RC, the high molecular weight form of the leucocyte common antigen. This antigen is found on B cells, approximately 50% of bone marrow cells, all CD8+ve T cells, but splits CD4+ve T cells into two populations,CD4+ CD45RC (Th 1-like) and CD4+ CD45RC low (Th-2 like). This product is routinely tested in flow cytometry on rat splenocytes.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% Bovine Serum Albumin
• Preservative: 0.09% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A purified

Alternate Names for CD45RC Antibody (OX-22) [FITC]

• B220
• CD45 antigen
• CD45R
• EC 3.1.3.48
• L-CA
• LY5
• protein tyrosine phosphatase, receptor type, C
• receptor-type tyrosine-protein phosphatase C
• T200 glycoprotein
• T200 leukocyte common antigen
• T200receptor type, c polypeptide

Background

CD45 is a family of single chain transmembraneous glycoproteins consisting of at least four isoforms (220, 205, 190, 180 kDa) which share a common large intracellular domain. Their extracellular domains are heavily glycosylated. The different isoforms are produced by alternative messenger RNA splicing of three exons of a single gene on chromosome 1. CD45 is expressed on cells of the human hematopoietic lineage (including hematopoietic stem cells) with the exception of mature red cells. It is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Antibodies recognising a common epitope on all of the isoforms are termed CD45 whilst those recognising only individual isoforms are termed CD45RA or CD45RO etc.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRC
• Uniprot:
  • P04157(Rat)