CD44 Antibody (A3D8)

Product Discontinued

CD44 Antibody (A3D8) Summary

• Immunogen: Purified from circulating malignant human Sezary T cells
• Marker: Cell Membrane Marker
• Specificity: Recognizes the CD44 (80-95 kDa) human cell surface glycoprotein on B and T lymphocytes, monocytes, granulocytes and red blood cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD44
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 ul/10^6 cells
  • Western Blot
• Application Notes: The epitope recognized by this antibody is sensitive to bromelain and other proteolytic enzymes. It is detectable in routine formalin-fixed, paraffin-embedded tissue. The antibody is also reactive with bone marrow nucleated cells, medullary thymocytes, liver Kupffer cells, fibroblasts, corneal cells, epidermal keratinocytes, synovial cells, a subset of pancreatic acinar cells and brain cells.Use in Western blot reported in scientific literature (PMID: 29111978).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS (pH 7.4) and 1.0% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for CD44 Antibody (A3D8)

• CD44 antigen
• CD44 molecule (Indian blood group)
• CD44
• CD44R
• CDw44
• cell surface glycoprotein CD44
• chondroitin sulfate proteoglycan 8
• CSPG8
• ECMR-III
• epican
• Extracellular matrix receptor III
• GP90 lymphocyte homing/adhesion receptor
• HCAM
• HCELL
• hematopoietic cell E- and L-selectin ligand
• Heparan sulfate proteoglycan
• Hermes antigen
• homing function and Indian blood group system
• HUTCH-I
• Hyaluronate receptor
• IN
• LHR
• MC56
• MDU2
• MDU2CD44 antigen (homing function and Indian blood group system)
• MDU3
• MDU3CDW44
• MIC4
• MIC4MGC10468
• MUTCH-I
• Pgp1
• PGP-1
• PGP-I
• Phagocytic glycoprotein 1
• Phagocytic glycoprotein I

Background

CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein 1 (pgp 1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD44 Antibody (NB600-1457)(3)

Publications using NB600-1457

• Zahreddine H. Survival of the fittest: understanding the role of eIF4E in cancer invasion and treatment evasion. Thesis. 2017-01-01 (FLOW, Human)
• Sieve I, Ricke-Hoch M, Kasten M, Battmer K. A positive feedback loop between IL-1b, LPS and NEU1 may promote atherosclerosis by enhancing a pro-inflammatory state in monocytes and macrophages. Vascul. Pharmacol. 2018-01-31 [PMID: 29371126] (Human)
• Zahreddine HA, Culjkovic-Kraljacic B, Emond A et al. The eukaryotic translation initiation factor eIF4E harnesses hyaluronan production to drive its malignant activity Elife. 2017-11-07 [PMID: 29111978] (WB, Human)

Bioinformatics

• Gene Symbol: CD44
• Entrez:
  • 960(Human)
• Uniprot:
  • P16070()