CD44 Antibody (740017) [Alexa Fluor® 488]

Rat splenocytes were stained with Mouse Anti-Rat CD4 APC-conjugated Monoclonal Antibody and either (A) Mouse Anti-Rat CD44 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB6577G) or (B) Mouse IgG2BAlexa Fluor 488 Isotype Control (Catalog # IC0041G). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Rt
• Applications: Flow
• Clone: 740017
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

CD44 Antibody (740017) [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant rat CD44
  Gln22-Thr223 (predicted)
  Accession # P26051
• Specificity: Detects rat CD44 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human, mouse, or porcine CD44 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD44
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD44 Antibody (740017) [Alexa Fluor® 488]

• CD44 antigen
• CD44 molecule (Indian blood group)
• CD44
• CD44R
• CDw44
• cell surface glycoprotein CD44
• chondroitin sulfate proteoglycan 8
• CSPG8
• ECMR-III
• Epican
• Extracellular matrix receptor III
• GP90 lymphocyte homing/adhesion receptor
• HCAM
• HCELL
• hematopoietic cell E- and L-selectin ligand
• Heparan sulfate proteoglycan
• Hermes antigen
• homing function and Indian blood group system
• HUTCH-I
• Hyaluronate receptor
• IN
• LHR
• MC56
• MDU2
• MDU2CD44 antigen (homing function and Indian blood group system)
• MDU3
• MDU3CDW44
• MIC4
• MIC4MGC10468
• MUTCH-I
• Pgp1
• PGP-1
• PGP-I
• Phagocytic glycoprotein 1
• Phagocytic glycoprotein I

Background

CD44 is a ubiquitously expressed protein that is a major receptor for hyaluronan and exerts control over cell growth and migration (1-5). Mouse CD44 has an extracellular domain (ECD) that contains a 100 amino acid (aa) hyaluronan-binding disulfide-stabilized link region, plus a 48-463 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. Within the stem, ten variably spliced exons (v1-10, exons 6-15) produce multiple protein isoforms (1‑5). The standard or hematopoietic form, CD44H, does not include the variable segments (1‑5). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (2, 4). With variable N- and O-glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1, 2). Within the N‑terminal invariant portion of the ECD (aa 23-222), rat CD44 shares 91% and 75% aa sequence identity with corresponding mouse and human CD44, respectively. The many reported functions of CD44 fall within three categories (1, 2). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 is a co-receptor that modifies activity of receptors including MET and the ErbB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, ezrin, radixin and moesin. CD44 can be synthesized in a soluble form or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases or ADAM proteases to produce soluble ectodomains (4, 6, 7). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta ‑like transmembrane portion and a cytoplasmic signaling molecule that affects gene expression (8, 9). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 2, 6).

1. Pure, E. and R.K. Assoian (2009) Cell. Signal. 21:651.
2. Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
3. Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160.
4. Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931.
5. Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603.
6. Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
7. Nakamura, H. et al. (2004) Cancer Res. 64:876.
8. Murakami, D. et al. (2003) Oncogene 22:1511.
9. Lammich, S. et al. (2002) J. Biol. Chem. 277:44754.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD44 Antibody (FAB6577G)(1)

Publication using FAB6577G

• YU WANG, GUOYING ZHU, JIANPING WANG, JUNXIANG CHEN Irradiation alters the differentiation potential of bone marrow mesenchymal stem cells Molecular Medicine Reports 2016-01-01 [PMID: 26572960]

Bioinformatics

• Gene Symbol: CD44
• Uniprot:
  • P26051()