CD4 Antibody


Western Blot: CD4 Antibody [NBP1-86143] - Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)
Immunohistochemistry-Paraffin: CD4 Antibody [NBP1-86143] - Staining of human tonsil shows moderate cytoplasmic positivity in lymphoid cells outside reaction centra.

Product Details

Reactivity Hu, DrSpecies Glossary
Applications WB, IHC, IHC-P, IHC-WhMt
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Order Details

CD4 Antibody Summary

Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS, pH 7.2, containing 40% glycerol
0.02% Sodium Azide
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Immunogen affinity purified


Application Notes
For IHC-Paraffin HIER pH6 retrieval is recommended. Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID 26635273)
Control Peptide
CD4 Protein (NBP1-86143PEP)
Read Publications using
NBP1-86143 in the following applications:

Reactivity Notes

Drosophila reactivity reported in scientific literature (PMID: 26635273).

Alternate Names for CD4 Antibody

  • CD4 antigen (p55)
  • CD4 antigen
  • CD4 molecule
  • CD4 receptor
  • CD4
  • CD4mut
  • T-cell surface antigen T4/Leu-3
  • T-cell surface glycoprotein CD4


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
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Applications: WB, ELISA, ICC/IF, S-ELISA
Species: Hu, Mu
Applications: Flow, IHC, ICC
Species: Mu
Applications: WB, Flow, Func, ICC/IF, IHC, IHC-Fr, IHC-P, IP, In vivo
Species: Hu
Applications: WB, Flow, AgAct
Species: Hu
Applications: Flow, ICC, Neut
Species: Rt
Applications: B/N, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IP, Flow-IC
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF
Species: Hu, Dr
Applications: WB, IHC, IHC-P, IHC-WhMt

Publications for CD4 Antibody (NBP1-86143)(2)

We have publications tested in 1 confirmed species: Drosophila.

We have publications tested in 1 application: IHC-WhMt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD4 Antibody (NBP1-86143). (Showing 1 - 1 of 1 FAQs).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at with any questions regarding this program.

Secondary Antibodies


Isotype Controls

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Blogs on CD4. Showing 1-10 of 12 blog posts - Show all blog posts.

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

CD20 (Cluster of differentiation 20, Membrane-spanning 4-domains subfamily A member 1 (MS4A1), CVID5, B-lymphocyte surface antigen B1)
CD20 is a human B-lymphocyte surface molecule that spans the membrane four times and is expressed on both normal and malignant cells. The CD20 antigen displays a unique expression pattern among hematopoietic cells - it is present on human pre B-ly...  Read full blog post.

CD8 alpha - Marker for cytotoxic T lymphocytes
The T-cell co-receptor CD8 is a cell-surface glycoprotein that bridges the lipid bilayer by interacting externally with ligands, such as major histocompatibility complex (MHC) class I, as well as internally with signaling molecules such as tyrosine ki...  Read full blog post.

Lymphocyte activation gene 3 (LAG3): No lag time in immune response
The LAG3 protein belongs to the Ig superfamily and contains 4 extracellular Ig-like domains (D1-D4). This molecule plays an key role in the immune response through negative regulation of T-cell proliferation, function, and homeostasis. It is required...  Read full blog post.

CD4 - An Important Co-receptor Assisting TCRs
The cluster of differentiation 4 (CD4) protein is a surface, type I membrane-embedded glycoprotein that is found on a wide range of cells: T-lymphocytes, B-cells, macrophages, granulocytes, and developmentally-dependent regions specific to the brain....  Read full blog post.

CD4, HIV and T Cell Signaling
CD4, also known as Cluster of Differentiation 4, interacts with major histocompatibility complex class II antigens, acts as a receptor for the human immunodeficiency virus and induces the aggregation of lipid rafts. It is expressed in T lymphocytes, B...  Read full blog post.

FOXP3: Master Regulatory Transcriptional Factor
FOXP3, a forkhead family transcription factor specially expressed in regulatory T (Treg) cells, controls the expression of many key immune-regulatory genes. Treg cells are a population of T lymphocytes that have critical roles in the immune system hom...  Read full blog post.

A Multicolored Approach to CD4 Antibody Assays
Novus Biologicals offers the widest selection of conjugated CD4 antibody products, with a database that's continually expanding to take into account the latest developments. This includes CD4 antibodies conjugated to Dynomics and PE fluorescent dyes,...  Read full blog post.

Showing 1-10 of 12 blog posts - Show all blog posts.

Contact Information

Product PDFs


Gene Symbol CD4

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