CD4 Antibody (YNB46.1.8 (Campath-9H)) [PE/Cy5.5] - Chimeric


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Product Details

Reactivity HuSpecies Glossary
Applications Flow
YNB46.1.8 (Campath-9H)

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CD4 Antibody (YNB46.1.8 (Campath-9H)) [PE/Cy5.5] - Chimeric Summary

Additional Information
Recombinant Monoclonal Antibody.
Rat T-cell line (NB2) transfected with the human CD4.
Recognizes the 55kDa human CD4 cell surface antigen, expressed by helper T lymphocytes and by monocytes.
IgG Kappa
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Flow Cytometry
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at if you have any questions.

Reactivity Notes


Packaging, Storage & Formulations

Store at 4C in the dark. Do not freeze.
0.05% Sodium Azide
Protein A purified

Alternate Names for CD4 Antibody (YNB46.1.8 (Campath-9H)) [PE/Cy5.5] - Chimeric

  • CD_antigen: CD4
  • CD4 antigen (p55)
  • CD4 antigen
  • CD4 molecule
  • CD4 receptor
  • CD4
  • CD4mut
  • T-cell surface antigen T4/Leu-3
  • T-cell surface glycoprotein CD4


CD4 (cluster of differentiation 4), also known as L3T4 or T4, is a 55 kDa single chain type I transmembrane glycoprotein belonging to the immunoglobin (Ig) superfamily. CD4 is predominantly expressed on most thymocytes, a subset of mature T lymphocytes, and weakly on monocytes, tissue macrophages, dendritic cells, and granulocytes. It is also expressed on neurons and glial cells in the brain (1). CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost giving rise to single positive (SP) CD4+ or CD8+ mature T cells. CD4+ SP cells (T helper cells) further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity (2). The extracellular region of CD4 consists of 372 amino acids (aa) with four immunoglobin-like domains (D1-D4). The structures of D1 and D3 resemble variable (IgV) domains while D2 and D4 resemble constant (IgC) domains (3).

Given its critical role in T cell development, CD4 also has diverse immunology-related functions. CD4 acts as a coreceptor with the T-cell receptor (TCR) during T cell activation and thymic differentiation by binding directly to major histocompatibility complex (MHC) class II antigens and associating with the protein tyrosine kinase, Lck (4). This interaction contributes to the formation of the immunological synapse (5). Defects in antigen presentation cause dysfunction of CD4+ T cells and the almost complete loss of MHC II expression on B cells in peripheral blood, as observed in severe combined immunodeficiency (SCID) (6). CD4 also functions as a receptor for the human immunodeficiency virus (HIV) by binding to gp120, the envelope glycoprotein of HIV-1. It has been shown that the V-like domains are critical for binding to gp120 (7). In immune mediated and infectious diseases of the central nervous system, CD4 functions as an indirect mediator of neuronal damage (8).


1. Omri, B., Crisanti, P., Alliot, F., Marty, M., Rutin, J., Levallois, C.,... Pessac, B. (1994). CD4 expression in neurons of the central nervous system. International Immunology, 6(3), 377-385. doi:10.1093/intimm/6.3.377

2. Wan, Y. Y., & Flavell, R. A. (2009). How diverse-CD4 effector T cells and their functions. Journal of Molecular Cell Biology, 1(1), 20-36. doi:10.1093/jmcb/mjp001

3. Wu, H., Myszka, D. G., Tendian, S. W., Brouillette, C. G., Sweet, R. W., Chaiken, I. M., & Hendrickson, W. A. (1996). Kinetic and structural analysis of mutant CD4 receptors that are defective in HIV gp120 binding. Proceedings of the National Academy of Sciences, 93(26), 15030-15035. doi:10.1073/pnas.93.26.15030

4. Doyle, C., & Strominger, J. L. (1987). Interaction between CD4 and class II MHC molecules mediates cell adhesion. Nature, 330, 256-259. doi:10.1038/330256a0

5. Vignali, D. A. (2010). CD4 on the road to coreceptor status. The Journal of Immunology, 184(11), 5933-5934. doi:10.4049/jimmunol.1090037

6. Tasher, D., & Dalal, I. (2012). The genetic basis of severe combined immunodeficiency and its variants. The Application of Clinical Genetics, 5, 67-80. doi:10.2147/tacg.s18693

7. Arthos, J., Deen, K. C., Chaikin, M. A., Fornwald, J. A., Sathe, G., Sattentau, Q. J.,... Sweet, R. W. (1989). Identification of the residues in human CD4 critical for the binding of HIV. Cell, 57(3), 469-481. doi:10.1016/0092-8674(89)90922-7

8. Buttini, M., Westland, C. E., Masliah, E., Yafeh, A. M., Wyss-Coray, T., Mucke, L. (1998). Novel role of human cd4 molecule identified in neurodegeneration. Nature Medicine, 4(4), 441-446. doi:10.1038/nm0498-441


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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FAQs for CD4 Antibody (NBP2-52658PECY55). (Showing 1 - 1 of 1 FAQ).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at with any questions regarding this program.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-52658AF350
Alexa Fluor 405 NBP2-52658AF405
Alexa Fluor 488 NBP2-52658AF488
Alexa Fluor 532 NBP2-52658AF532
Alexa Fluor 594 NBP2-52658AF594
Alexa Fluor 647 NBP2-52658AF647
Alexa Fluor 700 NBP2-52658AF700
Alexa Fluor 750 NBP2-52658AF750
Allophycocyanin NBP2-52658APC
Allophycocyanin/Cy7 NBP2-52658APCCY7
Biotin NBP2-52658B
DyLight 350 NBP2-52658UV
DyLight 405 NBP2-52658V
DyLight 488 NBP2-52658G
DyLight 550 NBP2-52658R
DyLight 650 NBP2-52658C
DyLight 680 NBP2-52658FR
DyLight 755 NBP2-52658IR
FITC NBP2-52658F
HRP NBP2-52658H
Janelia Fluor 549 NBP2-52658JF549
Janelia Fluor 646 NBP2-52658JF646
PE NBP2-52658PE
PE/Atto594 NBP2-52658PEATT594
PE/Cy5.5 NBP2-52658PECY55
PE/Cy7 NBP2-52658PECY7
PerCP NBP2-52658PCP

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Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

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Gene Symbol CD4