CD4 Antibody (CE9.1 (Clenoliximab)) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, PmSpecies Glossary
Applications WB, ELISA, Flow, IHC
Clone
CE9.1 (Clenoliximab)
Clonality
Monoclonal
Host
Human
Conjugate
Janelia Fluor 549

Order Details

CD4 Antibody (CE9.1 (Clenoliximab)) [Janelia Fluor® 549] Summary

Immunogen
Domain 1 of human CD4.
Specificity
The antibody binds to human CD4 with a Kd of 1.0 nM and an ED50 of 0.2 ug/ml.
Isotype
IgG4 Lambda
Clonality
Monoclonal
Host
Human
Gene
CD4
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for CD4 Antibody (CE9.1 (Clenoliximab)) [Janelia Fluor® 549]

  • CD_antigen: CD4
  • CD4 antigen (p55)
  • CD4 antigen
  • CD4 molecule
  • CD4 receptor
  • CD4
  • CD4mut
  • T-cell surface antigen T4/Leu-3
  • T-cell surface glycoprotein CD4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-75910JF549
Species: Hu, Pm
Applications: WB, ELISA, Flow, IHC

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CD4 Antibody (NBP2-75910JF549). (Showing 1 - 1 of 1 FAQ).

  1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
    • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-75910AF350
Alexa Fluor 405 NBP2-75910AF405
Alexa Fluor 488 NBP2-75910AF488
Alexa Fluor 532 NBP2-75910AF532
Alexa Fluor 594 NBP2-75910AF594
Alexa Fluor 647 NBP2-75910AF647
Alexa Fluor 700 NBP2-75910AF700
Alexa Fluor 750 NBP2-75910AF750
Allophycocyanin NBP2-75910APC
Allophycocyanin/Cy7 NBP2-75910APCCY7
Biotin NBP2-75910B
DyLight 350 NBP2-75910UV
DyLight 405 NBP2-75910V
DyLight 488 NBP2-75910G
DyLight 550 NBP2-75910R
DyLight 594 NBP2-75910DL594
DyLight 650 NBP2-75910C
DyLight 680 NBP2-75910FR
DyLight 755 NBP2-75910IR
FITC NBP2-75910F
HRP NBP2-75910H
Janelia Fluor 549 NBP2-75910JF549
Janelia Fluor 646 NBP2-75910JF646
PE NBP2-75910PE
PE/Atto594 NBP2-75910PEATT594
PE/Cy5.5 NBP2-75910PECY55
PE/Cy7 NBP2-75910PECY7
PerCP NBP2-75910PCP

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Bioinformatics Tool for CD4 Antibody (NBP2-75910JF549)

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Blogs on CD4. Showing 1-10 of 17 blog posts - Show all blog posts.

Success of combined IL-10 and IL-12 therapy in colon cancer depends on IFN-gamma and gut barrier integrity
By Jamshed Arslan, Pharm. D., PhD. Colon cancer is responsible for over 600,000 deaths per year worldwide. Colon cancer can be classified into two categories: mismatch repair (MMR)-deficient and MMR-proficient cancers...  Read full blog post.

mTOR Signaling and the Tumor Microenvironment
By Yoskaly Lazo-Fernandez, PhD The mammalian target of rapamycin (mTOR) is a conserved serine/threonine kinase that, as a member of two distinct intracellular protein complexes, mTORC1 and mTORC2, regulates protein...  Read full blog post.

Flow Cytometry Basics for the Non-Expert
By Jody Bonnevier, PhD.Flow cytometry: A Challenging Interdisciplinary ApplicationFlow cytometry has long been the cell analysis method of choice for immunologists, however more and more disciplines have recognize...  Read full blog post.

The role of MHC Class II RT1B and immune response post brain injury
The major histocompatibility complex (MHC) is responsible for binding peptide fragments arising from pathogens in order to display them on the cell surface for recognition from immune cells.  Once recognized, the foreign pathogen is typically evade...  Read full blog post.

Topics in CD11b: The innate immune response
Integrins are transmembrane receptors composed of alpha and beta chains, where beta-integrins are mainly expressed in leukocytes. Leukocytes are white blood cells that act in the immune system to defend our body against foreign pathogens.  Integrin...  Read full blog post.

Transferrin and the blood brain barrier
Transferrin, an iron binding protein that facilitates iron uptake in cells, is an integral part of a mechanism that may introduce antibody therapies to the central nervous system. Currently, the brain’s ability to take in antibody therapies i...  Read full blog post.

MHC Class I and the Herpes Simplex Virus
MHC molecules (also known as major histocompatibility complex molecules) assist in the presentation of antigens to T cells in order to eradicate foreign pathogens.  These molecules are highly polymorphic, meaning that they exist in multiple varian...  Read full blog post.

CD3 (OKT3) as a marker of immune response efficiency
Our immune system is a powerful defense mechanism against infection, however different variables can cause our immune response to work for or against us.  CD3 (cluster of differentiation 3) is one component of our immune signal response that is co...  Read full blog post.

CD20 (Cluster of differentiation 20, Membrane-spanning 4-domains subfamily A member 1 (MS4A1), CVID5, B-lymphocyte surface antigen B1)
CD20 is a human B-lymphocyte surface molecule that spans the membrane four times and is expressed on both normal and malignant cells. The CD20 antigen displays a unique expression pattern among hematopoietic cells - it is present on human pre B-ly...  Read full blog post.

CD8 alpha - Marker for cytotoxic T lymphocytes
The T-cell co-receptor CD8 is a cell-surface glycoprotein that bridges the lipid bilayer by interacting externally with ligands, such as major histocompatibility complex (MHC) class I, as well as internally with signaling molecules such as tyrosine ki...  Read full blog post.

Showing 1-10 of 17 blog posts - Show all blog posts.
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Bioinformatics

Gene Symbol CD4