CD31/PECAM-1 Antibody (C31.3)

Images

 
Immunohistochemistry-Frozen: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Analysis using Azide/BSA FREE version of NBP2-15202. Formalin-paraffin human Angiosarcoma stained with CD31 MAb (C31.3).
Immunohistochemistry-Paraffin: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Analysis using the Azide and BSA Free version of NBP2-15202. Staining of human tonsil stained with CD31 MAb (C31.3).
Flow Cytometry: CD31/PECAM-1 Antibody (C31.3) [NBP2-15202] - Human CD31 on Jurkat Cells. Black: Cells alone; Green: Isotype Control; Red: AF488-labeled CD31 monoclonal antibody (C31.3).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P
Clone
C31.3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

CD31/PECAM-1 Antibody (C31.3) Summary

Immunogen
Human recombinant CD31 protein
Localization
Cell surface and cytoplasm of endothelial cells
Marker
Endothelial Cell Marker
Specificity
CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PECAM1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
Application Notes
Human Chromosome Location: 17q23.3
Mol. Weight of Antigen: ~100kDa (endothelium) and ~130kDa (platelets)
Western blot note: The CD31 (PECAM-1) antibody recognizes CD31 as an ~100 kDa band in endothelium and an ~130 kDa band in platelets (Parums, 1990).
Immunohistology (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Immunohistochemistry-Frozen and Western Blot applications reported in literature.
Publications
Read Publications using NBP2-15202.

Reactivity Notes

Human, Weakly reacts with Rat.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for CD31/PECAM-1 Antibody (C31.3)

  • adhesion molecule
  • CD31 antigen
  • CD31
  • CD31/EndoCAM
  • endoCAM
  • FLJ34100
  • FLJ58394
  • GPIIA'
  • PECA1
  • PECAM1
  • PECAM-1
  • PECAM-1, CD31/EndoCAM
  • platelet endothelial cell adhesion molecule
  • platelet/endothelial cell adhesion molecule

Background

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules, and plays key roles in leukocyte migration, angiogenesis, and integrin activation. CD31 is expressed on endothelial and hematopoeitic (platelets, monocytes, macrophages, granulocytes, T and B lymphocytes, dendritic, bone marrow stem and adult) cells. The CD31 antibody stains these various cell types to various degrees (Parvens, 1990; Govender, 1997). The CD31 antibody (clone C31.3 or PECAM-1) is widely used as a pan-endothelial cell marker to demonstrate the presence of endothelial cells in tissue sections by immunohistochemistry. The CD31 (PECAM-1) antibody reacts with normal, benign, and malignant endothelium and has a number of practical applications including marking vessels and assessing tumor microvessel density (Giatromanolaki, 2012). Since malignant endothelium retains CD31 expression, the CD31 antibody is commonly used in antibody panels to determine or confirm tissue origin of a given tumor (Gratzinger, 2009). This can be particularly useful as it can otherwise be difficult to distinguish endothelial from other cell types in routine tissue sections solely by morphological features. Endothelial cells make up blood vessel lining, and angiogenesis refers to the growth of new blood vessels from pre-existing vessels. Pathological angiogenesis is associated with tumor growth and metastasis, and the CD31 antibody is useful for helping to confirm (CD31 / PECAM-1 antibody positive) or exclude (CD31 / PECAM-1 antibody negative) neoplastic angioinvasion (Jernman, 2012). The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 (PECAM-1) antibody staining may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CD31/PECAM-1 Antibody (NBP2-15202)(5)

We have publications tested in 4 applications: ICC/IF, IHC-Fr, IHC-P, WB.


Filter By Application
ICC/IF
(1)
IHC-Fr
(1)
IHC-P
(3)
WB
(1)
All Applications
Filter By Species
All Species
Showing Publications 1 - 5 of 5.
Publications using NBP2-15202 Applications Species
Giatromanolaki A, Koukourakis MI, Sivridis E et al. Vascular density analysis in colorectal cancer patients treated with vatalanib (PTK787/ZK222584) in the randomised CONFIRM trials. Br J Cancer. 2012 Sep 25 [PMID: 22910317]
Jernman J, Valimaki MJ, Louhimo J et al. The novel WHO 2010 classification for gastrointestinal neuroendocrine tumours correlates well with the metastatic potential of rectal neuroendocrine tumours. Neuroendocrinology. 2012 [PMID: 22327359] (IHC-P)

Details:
IHC (paraffin): Tables 1-3 (gastrointestinal neuroendocrine neoplasms).
IHC-P
Gratzinger D, Zhao S, West R et al. The transcription factor LMO2 is a robust marker of vascular endothelium and vascular neoplasms and selected other entities. Am J Clin Pathol. 2009 Feb [PMID: 19141387] (IHC-P)

Details:
IHC (P): Figs 2B (myocardial vasculature), 2D (fetal myocardial vasculature). Various other tissues were used in this study and results described.
IHC-P
Govender D, Harilal P, Dada M, Chetty R. CD31 (JC70) expression in plasma cells: an immunohistochemical analysis of reactive and neoplastic plasma cells. J Clin Pathol. 1997 Jun [PMID: 9378815] (IHC-P)

Details:
IHC (paraffin): Fig 1 (chronic inflammatory infiltrate), Fig 2 (multiple myeloma).
IHC-P
Parums DV, Cordell JL, Micklem K et al. JC70: a new monoclonal antibody that detects vascular endothelium associated antigen on routinely processed tissue sections. J Clin Pathol. 1990 Sep [PMID: 2212067] (WB, ICC/IF, IHC-Fr)

Details:
WB: Fig 1 (spleen and platelets); Immunocytochemistry: Fig 2 (blood smear); IHC (frozen): Fig 3 (tonsil); IHC (paraffin): Figs 4-7 (various tissues).
WB, ICC/IF, IHC-Fr

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for CD31/PECAM-1 Antibody (NBP2-15202) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on CD31/PECAM-1.

The application of CD31/Pecam-1 (MEC 7.46) in breast cancer research
CD31/PECAM-1, or platelet endothelial cell adhesion molecule 1, is a 130-kDa glycoprotein expressed on vascular and hematopoietic cells.  Depending on the cell type, CD31/PECAM-1 expression can be largely localized to cell junctions, playing a rol...  Read full blog post.

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Bioinformatics

Gene Symbol PECAM1
Entrez
Uniprot