CD19 Antibody

Images

 
Western Blot: CD19 Antibody [NBP2-15782] - A. 30 ug Raji whole cell lysate/extract 7.5 % SDS-PAGECD19 antibody dilution: 1:1000
Immunocytochemistry/ Immunofluorescence: CD19 Antibody [NBP2-15782] - Raji cells. Green: CD19 protein stained by CD19 antibody [C1C3]. Blue: Hoechst 33342 staining.
Flow Cytometry: CD19 Antibody [NBP2-15782] - Mouse splenocytes cell fixed in 4% paraformaldehyde at 4-C for 15 min. Black: Unlabelled sample was used as a control. Red: CD19 antibody [C1C3] dilution: 1:50. Acquisition ...read more
Western Blot: CD19 Antibody [NBP2-15782] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CD19 antibody [C1C3] diluted at 1:1000.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.67 mg/ml

Order Details

CD19 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human CD19. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CD19
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
Theoretical MW
61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Pig (86%), Bovine (82%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.67 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CD19 Antibody

  • B4
  • B-lymphocyte antigen CD19
  • B-lymphocyte surface antigen B4
  • CD19 antigen
  • CD19 molecule
  • CD19
  • CVID3
  • Differentiation antigen CD19
  • Leu-12
  • MGC12802
  • T-cell surface antigen Leu-12

Background

Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. This gene encodes a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on CD19.

CD19: An Undoubted Biomarker for B Cells
CD19 is a cell surface protein member of the large immunoglobulin superfamily that complexes with CD21, CD81, and CD225 in the membrane of mature B-cells. A major function of CD19 is to assemble with the antigen receptor of B-lymphocytes to decrease t...  Read full blog post.

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Bioinformatics

Gene Symbol CD19
Entrez
Uniprot