CD5 Antibody (OTI7A7) - Azide and BSA Free

Western Blot: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.

Immunocytochemistry/Immunofluorescence: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CD5.

Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - Staining of paraffin-embedded Human Kidney tissue using anti-CD5 mouse monoclonal antibody.

Flow Cytometry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-CD5 antibody, and then analyzed by flow cytometry.

Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CD5 mouse monoclonal antibody.

Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CD5 mouse monoclonal antibody.

Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CD5 mouse monoclonal antibody.

Flow Cytometry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - Analysis of Jurkat cells, using anti-CD5 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Imaging Mass Cytometry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - Human tonsil tissue, CD5 conjugated to Er167. IMC image submitted by a verified customer review.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready, MS
• Clone: OTI7A7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

CD5 Antibody (OTI7A7) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Imaging Mass Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Application Notes: This CD5 Antibody (OTI7A7) antibody is validated for Imaging Mass Cytometry from a verified customer review.
• Theoretical MW: 54.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for CD5 Antibody (OTI7A7) - Azide and BSA Free

• CD5 antigen (p56-62)
• CD5 antigen
• CD5 molecule
• CD5
• LEU1T-cell surface glycoprotein CD5
• Lymphocyte antigen T1/Leu-1
• T1

Background

In humans, CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for CD5 Antibody (NBP2-70360) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Alicia Gamboa IMC Human 04/09/2021

Summary

• Application: Imaging Mass Cytometry
• Sample Tested: Human Tonsil tissue
• Species: Human
• Lot: A002

Bioinformatics

• Gene Symbol: CD5